Reviewed, UniProtKB/Swiss-Prot P0AEX7 (LIVH_ECOLI)

Names and origin

Protein names

Recommended name:
    High-affinity branched-chain amino acid transport system permease protein livH
Alternative name(s):
    LIV-I protein H

Gene names

Name:	livH
Ordered Locus Names:	b3457, JW3422

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	High-affinity branched-chain amino acid transport system permease protein livH		PRO_0000060058
Regions
	Topological domain	1 – 21	21	Cytoplasmic Potential
	Transmembrane	22 – 42	21	Potential
	Topological domain	43 – 45	3	Periplasmic Potential
	Transmembrane	46 – 66	21	Potential
	Topological domain	67 – 68	2	Cytoplasmic Potential
	Transmembrane	69 – 89	21	Potential
	Topological domain	90 – 104	15	Periplasmic Potential
	Transmembrane	105 – 125	21	Potential
	Topological domain	126 – 154	29	Cytoplasmic Potential
	Transmembrane	155 – 175	21	Potential
	Topological domain	176 – 203	28	Periplasmic Potential
	Transmembrane	204 – 224	21	Potential
	Topological domain	225 – 245	21	Cytoplasmic Potential
	Transmembrane	246 – 266	21	Potential
	Topological domain	267 – 280	14	Periplasmic Potential
	Transmembrane	281 – 301	21	Potential
	Topological domain	302 – 308	7	Cytoplasmic Potential
Experimental info
	Sequence conflict	253	1	S → G Ref.1
	Sequence conflict	253	1	S → G Ref.2
	Sequence conflict	288	1	L → P Ref.1
	Sequence conflict	288	1	L → P Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0AEX7-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 8C8A4BD56718A4BE	FASTA	308	32,982
	10 20 30 40 50 60 MSEQFLYFLQ QMFNGVTLGS TYALIAIGYT MVYGIIGMIN FAHGEVYMIG SYVSFMIIAA 70 80 90 100 110 120 LMMMGIDTGW LLVAAGFVGA IVIASAYGWS IERVAYRPVR NSKRLIALIS AIGMSIFLQN 130 140 150 160 170 180 YVSLTEGSRD VALPSLFNGQ WVVGHSENFS ASITTMQAVI WIVTFLAMLA LTIFIRYSRM 190 200 210 220 230 240 GRACRACAED LKMASLLGIN TDRVIALTFV IGAAMAAVAG VLLGQFYGVI NPYIGFMAGM 250 260 270 280 290 300 KAFTAAVLGG IGSIPGAMIG GLILGIAEAL SSAYLSTEYK DVVSFALLIL VLLVMPTGIL GRPEVEKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of livH, the structural gene encoding a component of the leucine transport system in Escherichia coli." Nazos P.M., Antonucci T.K., Landick R., Oxender D.L. J. Bacteriol. 166:565-573(1986) [PubMed: 3009409] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence and genetic characterization reveal six essential genes for the LIV-I and LS transport systems of Escherichia coli." Adams M.D., Wagner L.M., Graddis T.J., Landick R., Antonucci T.K., Gibson A.L., Oxender D.L. J. Biol. Chem. 265:11436-11443(1990) [PubMed: 2195019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05516 Genomic DNA. Translation: AAA83884.1. U00039 Genomic DNA. Translation: AAB18432.1. U00096 Genomic DNA. Translation: AAC76482.1. AP009048 Genomic DNA. Translation: BAE77836.1.
PIR	QRECLH. S47676.
RefSeq	AP_004335.1. NP_417914.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P0AEX7.
Protein family/group databases
TCDB	3.A.1.4.1. ATP-binding cassette (ABC) superfamily.
Genome annotation databases
GeneID	947965.
GenomeReviews	Gene locus JW3422 in contig AP009048_GR. Gene locus b3457 in contig U00096_GR.
KEGG	ecj:JW3422. eco:b3457.
Organism-specific databases
EchoBASE	EB0533.
EcoGene	EG10538. livH.
CMR	Search...
Phylogenomic databases
eggNOG	COG0559.
HOGENOM	HBG728273.
OMA	TTMQLVI.
Enzyme and pathway databases
BioCyc	EcoCyc:LIVH-MONOMER. ECOL168927:B3457-MONOMER.
Gene expression databases
Genevestigator	P0AEX7.
Family and domain databases
InterPro	IPR001851. Bac_inmem_transp. [Graphical view]
Pfam	PF02653. BPD_transp_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIVH_ECOLI

Accession

Primary (citable) accession number: P0AEX7
Secondary accession number(s): P08340, Q2M7C0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	December 20, 2005
Last modified:	January 19, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)