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Protein

1-phosphofructokinase

Gene

fruK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + D-fructose 1-phosphate = ADP + D-fructose 1,6-bisphosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei255 – 2551Sequence analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi187 – 1904ATPSequence analysis

GO - Molecular functioni

  • 1-phosphofructokinase activity Source: EcoCyc
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • carbohydrate phosphorylation Source: GOC
  • fructose catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:1-PFK-MONOMER.
ECOL316407:JW2155-MONOMER.
MetaCyc:1-PFK-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphofructokinase (EC:2.7.1.56)
Alternative name(s):
Fructose 1-phosphate kinase
Gene namesi
Name:fruK
Synonyms:fpk
Ordered Locus Names:b2168, JW2155
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10337. fruK.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3123121-phosphofructokinasePRO_0000080076Add
BLAST

Proteomic databases

PaxDbiP0AEW9.
PRIDEiP0AEW9.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262911. 7 interactions.
IntActiP0AEW9. 2 interactions.
STRINGi511145.b2168.

Structurei

3D structure databases

ProteinModelPortaliP0AEW9.
SMRiP0AEW9. Positions 4-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family.Curated

Phylogenomic databases

eggNOGiENOG4105DE4. Bacteria.
COG1105. LUCA.
HOGENOMiHOG000265280.
InParanoidiP0AEW9.
KOiK00882.
OMAiNIHEFKC.
OrthoDBiEOG61KBKF.
PhylomeDBiP0AEW9.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR022463. 1-PFruKinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011611. PfkB_dom.
IPR029056. Ribokinase-like.
IPR017583. Tagatose/fructose_Pkinase.
[Graphical view]
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
PIRSFiPIRSF000535. 1PFK/6PFK/LacC. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR03168. 1-PFK. 1 hit.
TIGR03828. pfkB. 1 hit.
PROSITEiPS00583. PFKB_KINASES_1. 1 hit.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRVATITL NPAYDLVGFC PEIERGEVNL VKTTGLHAAG KGINVAKVLK
60 70 80 90 100
DLGIDVTVGG FLGKDNQDGF QQLFSELGIA NRFQVVQGRT RINVKLTEKD
110 120 130 140 150
GEVTDFNFSG FEVTPADWER FVTDSLSWLG QFDMVCVSGS LPSGVSPEAF
160 170 180 190 200
TDWMTRLRSQ CPCIIFDSSR EALVAGLKAA PWLVKPNRRE LEIWAGRKLP
210 220 230 240 250
EMKDVIEAAH ALREQGIAHV VISLGAEGAL WVNASGEWIA KPPSVDVVST
260 270 280 290 300
VGAGDSMVGG LIYGLLMRES SEHTLRLATA VAALAVSQSN VGITDRPQLA
310
AMMARVDLQP FN
Length:312
Mass (Da):33,756
Last modified:December 20, 2005 - v1
Checksum:i067E5CF7C584FEA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53948 Genomic DNA. Translation: CAA37896.1.
U00007 Genomic DNA. Translation: AAA60525.1.
U00096 Genomic DNA. Translation: AAC75229.1.
AP009048 Genomic DNA. Translation: BAA15977.1.
PIRiB37245.
RefSeqiNP_416673.1. NC_000913.3.
WP_000091263.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75229; AAC75229; b2168.
BAA15977; BAA15977; BAA15977.
GeneIDi946676.
KEGGiecj:JW2155.
eco:b2168.
PATRICi32119685. VBIEscCol129921_2253.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53948 Genomic DNA. Translation: CAA37896.1.
U00007 Genomic DNA. Translation: AAA60525.1.
U00096 Genomic DNA. Translation: AAC75229.1.
AP009048 Genomic DNA. Translation: BAA15977.1.
PIRiB37245.
RefSeqiNP_416673.1. NC_000913.3.
WP_000091263.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AEW9.
SMRiP0AEW9. Positions 4-287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262911. 7 interactions.
IntActiP0AEW9. 2 interactions.
STRINGi511145.b2168.

Proteomic databases

PaxDbiP0AEW9.
PRIDEiP0AEW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75229; AAC75229; b2168.
BAA15977; BAA15977; BAA15977.
GeneIDi946676.
KEGGiecj:JW2155.
eco:b2168.
PATRICi32119685. VBIEscCol129921_2253.

Organism-specific databases

EchoBASEiEB0333.
EcoGeneiEG10337. fruK.

Phylogenomic databases

eggNOGiENOG4105DE4. Bacteria.
COG1105. LUCA.
HOGENOMiHOG000265280.
InParanoidiP0AEW9.
KOiK00882.
OMAiNIHEFKC.
OrthoDBiEOG61KBKF.
PhylomeDBiP0AEW9.

Enzyme and pathway databases

BioCyciEcoCyc:1-PFK-MONOMER.
ECOL316407:JW2155-MONOMER.
MetaCyc:1-PFK-MONOMER.

Miscellaneous databases

PROiP0AEW9.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR022463. 1-PFruKinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011611. PfkB_dom.
IPR029056. Ribokinase-like.
IPR017583. Tagatose/fructose_Pkinase.
[Graphical view]
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
PIRSFiPIRSF000535. 1PFK/6PFK/LacC. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR03168. 1-PFK. 1 hit.
TIGR03828. pfkB. 1 hit.
PROSITEiPS00583. PFKB_KINASES_1. 1 hit.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureus."
    Orchard L.M.D., Kornberg H.L.
    Proc. R. Soc. B 242:87-90(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / BHB2600.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiK1PF_ECOLI
AccessioniPrimary (citable) accession number: P0AEW9
Secondary accession number(s): P23539
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: January 20, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.