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Protein

1-phosphofructokinase

Gene

fruK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + D-fructose 1-phosphate = ADP + D-fructose 1,6-bisphosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei255Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi187 – 190ATPSequence analysis4

GO - Molecular functioni

  • 1-phosphofructokinase activity Source: EcoCyc
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • fructose catabolic process Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:1-PFK-MONOMER.
MetaCyc:1-PFK-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphofructokinase (EC:2.7.1.56)
Alternative name(s):
Fructose 1-phosphate kinase
Gene namesi
Name:fruK
Synonyms:fpk
Ordered Locus Names:b2168, JW2155
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10337. fruK.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000800761 – 3121-phosphofructokinaseAdd BLAST312

Proteomic databases

PaxDbiP0AEW9.
PRIDEiP0AEW9.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262911. 7 interactors.
IntActiP0AEW9. 2 interactors.
STRINGi316385.ECDH10B_2325.

Structurei

3D structure databases

ProteinModelPortaliP0AEW9.
SMRiP0AEW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family.Curated

Phylogenomic databases

eggNOGiENOG4105DE4. Bacteria.
COG1105. LUCA.
HOGENOMiHOG000265280.
InParanoidiP0AEW9.
KOiK00882.
PhylomeDBiP0AEW9.

Family and domain databases

CDDicd01164. FruK_PfkB_like. 1 hit.
Gene3Di2.20.150.10. 1 hit.
3.40.1190.20. 1 hit.
InterProiView protein in InterPro
IPR022463. 1-PFruKinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011611. PfkB_dom.
IPR029056. Ribokinase-like.
IPR023314. T6P/1PhK_N_dom.
IPR017583. Tagatose/fructose_Pkinase.
PfamiView protein in Pfam
PF00294. PfkB. 1 hit.
PIRSFiPIRSF000535. 1PFK/6PFK/LacC. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR03168. 1-PFK. 1 hit.
TIGR03828. pfkB. 1 hit.
PROSITEiView protein in PROSITE
PS00583. PFKB_KINASES_1. 1 hit.
PS00584. PFKB_KINASES_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AEW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRVATITL NPAYDLVGFC PEIERGEVNL VKTTGLHAAG KGINVAKVLK
60 70 80 90 100
DLGIDVTVGG FLGKDNQDGF QQLFSELGIA NRFQVVQGRT RINVKLTEKD
110 120 130 140 150
GEVTDFNFSG FEVTPADWER FVTDSLSWLG QFDMVCVSGS LPSGVSPEAF
160 170 180 190 200
TDWMTRLRSQ CPCIIFDSSR EALVAGLKAA PWLVKPNRRE LEIWAGRKLP
210 220 230 240 250
EMKDVIEAAH ALREQGIAHV VISLGAEGAL WVNASGEWIA KPPSVDVVST
260 270 280 290 300
VGAGDSMVGG LIYGLLMRES SEHTLRLATA VAALAVSQSN VGITDRPQLA
310
AMMARVDLQP FN
Length:312
Mass (Da):33,756
Last modified:December 20, 2005 - v1
Checksum:i067E5CF7C584FEA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53948 Genomic DNA. Translation: CAA37896.1.
U00007 Genomic DNA. Translation: AAA60525.1.
U00096 Genomic DNA. Translation: AAC75229.1.
AP009048 Genomic DNA. Translation: BAA15977.1.
PIRiB37245.
RefSeqiNP_416673.1. NC_000913.3.
WP_000091263.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75229; AAC75229; b2168.
BAA15977; BAA15977; BAA15977.
GeneIDi946676.
KEGGiecj:JW2155.
eco:b2168.
PATRICifig|1411691.4.peg.71.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiK1PF_ECOLI
AccessioniPrimary (citable) accession number: P0AEW9
Secondary accession number(s): P23539
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: July 5, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families