P0AEW6 (INGK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inosine-guanosine kinase
EC=2.7.1.73

Gene names

Name:	gsk
Ordered Locus Names:	b0477, JW0466

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + inosine = ADP + IMP.
Pathway	Purine metabolism; IMP biosynthesis via salvage pathway; IMP from inosine: step 1/1.
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.
Sequence caution	The sequence BAA14306.1 differs from that shown. Reason: Frameshift at position 281.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydrolase activity, acting on glycosyl bonds Inferred from electronic annotation. Source: InterPro inosine kinase activity Inferred from direct assay Ref.2. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Inosine-guanosine kinase		PRO_0000080070
Experimental info
	Sequence conflict	314	1	A → V in BAA14306. Ref.6
	Sequence conflict	326	1	R → P in AAC36932. Ref.1
	Sequence conflict	365 – 366	2	LH → PD in AAC36932. Ref.1
	Sequence conflict	389	1	L → S in AAC36932. Ref.1
	Sequence conflict	421 – 423	3	PER → AV in AAC36932. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0AEW6 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 04693F70D1540236	FASTA	434	48,449
	10 20 30 40 50 60 MKFPGKRKSK HYFPVNARDP LLQQFQPENE TSAAWVVGID QTLVDIEAKV DDEFIERYGL 70 80 90 100 110 120 SAGHSLVIED DVAEALYQEL KQKNLITHQF AGGTIGNTMH NYSVLADDRS VLLGVMCSNI 130 140 150 160 170 180 EIGSYAYRYL CNTSSRTDLN YLQGVDGPIG RCFTLIGESG ERTFAISPGH MNQLRAESIP 190 200 210 220 230 240 EDVIAGASAL VLTSYLVRCK PGEPMPEATM KAIEYAKKYN VPVVLTLGTK FVIAENPQWW 250 260 270 280 290 300 QQFLKDHVSI LAMNEDEAEA LTGESDPLLA SDKALDWVDL VLCTAGPIGL YMAGFTEDEA 310 320 330 340 350 360 KRKTQHPLLP GAIAEFNQYE FSRAMRHKDC QNPLRVYSHI APYMGGPEKI MNTNGAGDGA 370 380 390 400 410 420 LAALLHDITA NSYHRSNVPN SSKHKFTWLT YSSLAQVCKY ANRVSYQVLN QHSPRLTRGL 430 PEREDSLEES YWDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the gsk gene encoding guanosine kinase of Escherichia coli." Harlow K.W., Nygaard P., Hove-Jensen B. J. Bacteriol. 177:2236-2240(1995) [PubMed: 7721718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, CHARACTERIZATION. Strain: K12.
[2]	"Cloning of a guanosine-inosine kinase gene of Escherichia coli and characterization of the purified gene product." Mori H., Iida A., Teshiba S., Fujio T. J. Bacteriol. 177:4921-4926(1995) [PubMed: 7665468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Isolation and characterization of visible light-sensitive mutants of Escherichia coli K12." Miyamoto K., Nakahigashi K., Nishimura K., Inokuchi H. J. Mol. Biol. 219:393-398(1991) [PubMed: 2051480] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-316. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L35149 Genomic DNA. Translation: AAC36932.1. D00798 Genomic DNA. Translation: BAA00690.1. U82664 Genomic DNA. Translation: AAB40231.1. U00096 Genomic DNA. Translation: AAC73579.1. AP009048 Genomic DNA. Translation: BAE76256.1. D90259 Genomic DNA. Translation: BAA14306.1. Frameshift.
PIR	JQ0812.
RefSeq	NP_415010.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AEW6.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48148N.
IntAct	P0AEW6. 8 interactions.
MINT	MINT-1264409.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000647; EBESCP00000000647; EBESCG00000000540. EBESCT00000014520; EBESCP00000013811; EBESCG00000013581.
GeneID	946584.
GenomeReviews	Gene locus JW0466 in contig AP009048_GR. Gene locus b0477 in contig U00096_GR.
KEGG	ecj:JW0466. eco:b0477.
PATRIC	32116111. VBIEscCol129921_0497.
Organism-specific databases
EchoBASE	EB1094.
EcoGene	EG11102. gsk.
Phylogenomic databases
eggNOG	COG0524.
GeneTree	EBGT00050000011151.
HOGENOM	HBG298340.
OMA	IRNTNGA.
PhylomeDB	P0AEW6.
ProtClustDB	PRK15074.
Enzyme and pathway databases
BioCyc	EcoCyc:GSK-MONOMER. MetaCyc:GSK-MONOMER.
Gene expression databases
Genevestigator	P0AEW6.
Family and domain databases
InterPro	IPR011611. Carb/pur_kinase. IPR002173. Carboh/pur_kinase_PfkB_CS. IPR007342. PseudoU_P_glycosidase. [Graphical view]
KO	K00892.
PANTHER	PTHR10584:SF1. Indigdn_synthA. 1 hit.
Pfam	PF00294. PfkB. 1 hit. [Graphical view]
PROSITE	PS00583. PFKB_KINASES_1. False negative. PS00584. PFKB_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INGK_ECOLI

Accession

Primary (citable) accession number: P0AEW6
Secondary accession number(s): P22937, Q2MBV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program