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Protein

Inosine-guanosine kinase

Gene

gsk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + inosine = ADP + IMP.

Pathwayi: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from inosine.
Proteins known to be involved in this subpathway in this organism are:
  1. Inosine-guanosine kinase (gsk)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from inosine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • inosine kinase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GSK-MONOMER
MetaCyc:GSK-MONOMER
UniPathwayiUPA00591; UER00647

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-guanosine kinase (EC:2.7.1.73)
Gene namesi
Name:gsk
Ordered Locus Names:b0477, JW0466
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11102 gsk

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000800701 – 434Inosine-guanosine kinaseAdd BLAST434

Proteomic databases

PaxDbiP0AEW6
PRIDEiP0AEW6

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ybhKP757675EBI-548746,EBI-550390

Protein-protein interaction databases

BioGridi4259850, 4 interactors
DIPiDIP-48148N
IntActiP0AEW6, 9 interactors
STRINGi316385.ECDH10B_0433

Structurei

3D structure databases

ProteinModelPortaliP0AEW6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family.Curated

Phylogenomic databases

eggNOGiENOG4107RE1 Bacteria
COG0524 LUCA
HOGENOMiHOG000278653
InParanoidiP0AEW6
KOiK00892
OMAiLLGVMCN
PhylomeDBiP0AEW6

Family and domain databases

InterProiView protein in InterPro
IPR002173 Carboh/pur_kinase_PfkB_CS
IPR011611 PfkB_dom
IPR029056 Ribokinase-like
PfamiView protein in Pfam
PF00294 PfkB, 1 hit
SUPFAMiSSF53613 SSF53613, 2 hits
PROSITEiView protein in PROSITE
PS00584 PFKB_KINASES_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0AEW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFPGKRKSK HYFPVNARDP LLQQFQPENE TSAAWVVGID QTLVDIEAKV
60 70 80 90 100
DDEFIERYGL SAGHSLVIED DVAEALYQEL KQKNLITHQF AGGTIGNTMH
110 120 130 140 150
NYSVLADDRS VLLGVMCSNI EIGSYAYRYL CNTSSRTDLN YLQGVDGPIG
160 170 180 190 200
RCFTLIGESG ERTFAISPGH MNQLRAESIP EDVIAGASAL VLTSYLVRCK
210 220 230 240 250
PGEPMPEATM KAIEYAKKYN VPVVLTLGTK FVIAENPQWW QQFLKDHVSI
260 270 280 290 300
LAMNEDEAEA LTGESDPLLA SDKALDWVDL VLCTAGPIGL YMAGFTEDEA
310 320 330 340 350
KRKTQHPLLP GAIAEFNQYE FSRAMRHKDC QNPLRVYSHI APYMGGPEKI
360 370 380 390 400
MNTNGAGDGA LAALLHDITA NSYHRSNVPN SSKHKFTWLT YSSLAQVCKY
410 420 430
ANRVSYQVLN QHSPRLTRGL PEREDSLEES YWDR
Length:434
Mass (Da):48,449
Last modified:December 20, 2005 - v1
Checksum:i04693F70D1540236
GO

Sequence cautioni

The sequence BAA14306 differs from that shown. Reason: Frameshift at position 281.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti314A → V in BAA14306 (PubMed:2051480).Curated1
Sequence conflicti326R → P in AAC36932 (PubMed:7721718).Curated1
Sequence conflicti365 – 366LH → PD in AAC36932 (PubMed:7721718).Curated2
Sequence conflicti389L → S in AAC36932 (PubMed:7721718).Curated1
Sequence conflicti421 – 423PER → AV in AAC36932 (PubMed:7721718).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35149 Genomic DNA Translation: AAC36932.1
D00798 Genomic DNA Translation: BAA00690.1
U82664 Genomic DNA Translation: AAB40231.1
U00096 Genomic DNA Translation: AAC73579.1
AP009048 Genomic DNA Translation: BAE76256.1
D90259 Genomic DNA Translation: BAA14306.1 Frameshift.
PIRiJQ0812
RefSeqiNP_415010.1, NC_000913.3
WP_000671574.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73579; AAC73579; b0477
BAE76256; BAE76256; BAE76256
GeneIDi946584
KEGGiecj:JW0466
eco:b0477
PATRICifig|1411691.4.peg.1799

Similar proteinsi

Entry informationi

Entry nameiINGK_ECOLI
AccessioniPrimary (citable) accession number: P0AEW6
Secondary accession number(s): P22937, Q2MBV0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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