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Reviewed, UniProtKB/Swiss-Prot P0AEW6 (INGK_ECOLI)

Last modified January 19, 2010. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Inosine-guanosine kinase
    EC=2.7.1.73
Gene names
Name: gsk
Ordered Locus Names: b0477, JW0466
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

ATP + inosine = ADP + IMP.

Pathway

Purine metabolism; IMP biosynthesis via salvage pathway; IMP from inosine: step 1/1.

Sequence similarities

Belongs to the carbohydrate kinase pfkB family.

Sequence caution

The sequence BAA14306.1 differs from that shown. Reason: Frameshift at position 281.

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Ontologies

Keywords
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

inosine kinase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 434434Inosine-guanosine kinase
PRO_0000080070

Experimental info

Sequence conflict3141A → V in BAA14306. Ref.6
Sequence conflict3261R → P in AAC36932. Ref.1
Sequence conflict365 – 3662LH → PD in AAC36932. Ref.1
Sequence conflict3891L → S in AAC36932. Ref.1
Sequence conflict421 – 4233PER → AV in AAC36932. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P0AEW6-1 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 04693F70D1540236

FASTA43448,449
        10         20         30         40         50         60 
MKFPGKRKSK HYFPVNARDP LLQQFQPENE TSAAWVVGID QTLVDIEAKV DDEFIERYGL 

        70         80         90        100        110        120 
SAGHSLVIED DVAEALYQEL KQKNLITHQF AGGTIGNTMH NYSVLADDRS VLLGVMCSNI 

       130        140        150        160        170        180 
EIGSYAYRYL CNTSSRTDLN YLQGVDGPIG RCFTLIGESG ERTFAISPGH MNQLRAESIP 

       190        200        210        220        230        240 
EDVIAGASAL VLTSYLVRCK PGEPMPEATM KAIEYAKKYN VPVVLTLGTK FVIAENPQWW 

       250        260        270        280        290        300 
QQFLKDHVSI LAMNEDEAEA LTGESDPLLA SDKALDWVDL VLCTAGPIGL YMAGFTEDEA 

       310        320        330        340        350        360 
KRKTQHPLLP GAIAEFNQYE FSRAMRHKDC QNPLRVYSHI APYMGGPEKI MNTNGAGDGA 

       370        380        390        400        410        420 
LAALLHDITA NSYHRSNVPN SSKHKFTWLT YSSLAQVCKY ANRVSYQVLN QHSPRLTRGL 

       430 
PEREDSLEES YWDR

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References

« Hide 'large scale' references
[1]"Cloning and characterization of the gsk gene encoding guanosine kinase of Escherichia coli."
Harlow K.W., Nygaard P., Hove-Jensen B.
J. Bacteriol. 177:2236-2240(1995) [PubMed: 7721718] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, CHARACTERIZATION.
Strain: K12.
[2]"Cloning of a guanosine-inosine kinase gene of Escherichia coli and characterization of the purified gene product."
Mori H., Iida A., Teshiba S., Fujio T.
J. Bacteriol. 177:4921-4926(1995) [PubMed: 7665468] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Isolation and characterization of visible light-sensitive mutants of Escherichia coli K12."
Miyamoto K., Nakahigashi K., Nishimura K., Inokuchi H.
J. Mol. Biol. 219:393-398(1991) [PubMed: 2051480] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-316.
Strain: K12.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L35149 Genomic DNA. Translation: AAC36932.1.
D00798 Genomic DNA. Translation: BAA00690.1.
U82664 Genomic DNA. Translation: AAB40231.1.
U00096 Genomic DNA. Translation: AAC73579.1.
AP009048 Genomic DNA. Translation: BAE76256.1.
D90259 Genomic DNA. Translation: BAA14306.1. Frameshift.
PIRJQ0812.
RefSeqAP_001126.1.
NP_415010.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGP0AEW6.

Genome annotation databases

GeneID946584.
GenomeReviewsGene locus JW0466 in contig AP009048_GR.
Gene locus b0477 in contig U00096_GR.
KEGGecj:JW0466.
eco:b0477.

Organism-specific databases

EchoBASEEB1094.
EcoGeneEG11102. gsk.
CMRSearch...

Phylogenomic databases

eggNOGCOG0524.
HOGENOMHBG298340.
OMAIRNTNGA.

Enzyme and pathway databases

BioCycEcoCyc:GSK-MONOMER.
ECOL168927:B0477-MONOMER.
MetaCyc:GSK-MONOMER.

Gene expression databases

GenevestigatorP0AEW6.

Family and domain databases

InterProIPR011611. Carb/pur_kinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
[Graphical view]
PfamPF00294. PfkB. 1 hit.
[Graphical view]
PROSITEPS00583. PFKB_KINASES_1. False negative.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameINGK_ECOLI
AccessionPrimary (citable) accession number: P0AEW6
Secondary accession number(s): P22937, Q2MBV0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: January 19, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents