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Protein

3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA

Gene

cpdA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Hydrolyzes cAMP to 5'-AMP. Plays an important regulatory role in modulating the intracellular concentration of cAMP, thereby influencing cAMP-dependent processes.UniRule annotation

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.UniRule annotation

Cofactori

a metal cationUniRule annotationNote: Binds 2 metal cations per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi22 – 221Metal cation 1UniRule annotation
Metal bindingi24 – 241Metal cation 1UniRule annotation
Binding sitei24 – 241cAMPUniRule annotation
Metal bindingi64 – 641Metal cation 1UniRule annotation
Metal bindingi64 – 641Metal cation 2UniRule annotation
Binding sitei64 – 641cAMPUniRule annotation
Metal bindingi94 – 941Metal cation 2UniRule annotation
Metal bindingi164 – 1641Metal cation 2UniRule annotation
Metal bindingi203 – 2031Metal cation 2UniRule annotation
Metal bindingi205 – 2051Metal cation 1UniRule annotation
Binding sitei205 – 2051cAMPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi94 – 952cAMPUniRule annotation

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-HAMAP
  3. nucleotide binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-3879-MONOMER.
ECOO157:ICC-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
3',5'-cyclic adenosine monophosphate phosphodiesterase CpdAUniRule annotation (EC:3.1.4.17UniRule annotation)
Short name:
3',5'-cyclic AMP phosphodiesteraseUniRule annotation
Short name:
cAMP phosphodiesteraseUniRule annotation
Gene namesi
Name:cpdAUniRule annotation
Synonyms:icc
Ordered Locus Names:Z4389, ECs3920
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558 Componenti: Chromosome UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2752753',5'-cyclic adenosine monophosphate phosphodiesterase CpdAPRO_0000084147Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi155864.Z4389.

Structurei

3D structure databases

ProteinModelPortaliP0AEW5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cAMP phosphodiesterase class-III family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000238351.
KOiK03651.
OMAiSEYQLEW.
OrthoDBiEOG6QG8GQ.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
HAMAPiMF_00905. cAMP_phophodiest_CpdA.
InterProiIPR013622. Calcineurin-like_phos_C.
IPR004843. Calcineurin-like_PHP_apaH.
IPR026575. cAMP_Pdiest_CpdA.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
ProDomiPD587589. Calcineurin-like_phos_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AEW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLLTLPLA GEARVRILQI TDTHLFAQKH EALLGVNTWE SYQAVLEAIR
60 70 80 90 100
PHQHEFDLIV ATGDLAQDQS SAAYQHFAEG IASFRAPCVW LPGNHDFQPA
110 120 130 140 150
MYSALQDAGI SPAKRVFIGE QWQILLLDSQ VFGVPHGELS EFQLEWLERK
160 170 180 190 200
LADAPERHTL LLLHHHPLPA GCSWLDQHSL RNAGELDTVL AKFPHVKYLL
210 220 230 240 250
CGHIHQELDL DWNGRRLLAT PSTCVQFKPH CSNFTLDTIA PGWRTLELHA
260 270
DGTLTTEVHR LADTRFQPDT ASEGY
Length:275
Mass (Da):30,938
Last modified:December 20, 2005 - v1
Checksum:i97696478536CFBF1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58171.1.
BA000007 Genomic DNA. Translation: BAB37343.1.
PIRiG85963.
H91118.
RefSeqiNP_289612.1. NC_002655.2.
NP_311947.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG58171; AAG58171; Z4389.
BAB37343; BAB37343; BAB37343.
GeneIDi916255.
958507.
KEGGiece:Z4389.
ecs:ECs3920.
PATRICi18357331. VBIEscCol44059_3843.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58171.1.
BA000007 Genomic DNA. Translation: BAB37343.1.
PIRiG85963.
H91118.
RefSeqiNP_289612.1. NC_002655.2.
NP_311947.1. NC_002695.1.

3D structure databases

ProteinModelPortaliP0AEW5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z4389.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG58171; AAG58171; Z4389.
BAB37343; BAB37343; BAB37343.
GeneIDi916255.
958507.
KEGGiece:Z4389.
ecs:ECs3920.
PATRICi18357331. VBIEscCol44059_3843.

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000238351.
KOiK03651.
OMAiSEYQLEW.
OrthoDBiEOG6QG8GQ.

Enzyme and pathway databases

BioCyciECOL386585:GJFA-3879-MONOMER.
ECOO157:ICC-MONOMER.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
HAMAPiMF_00905. cAMP_phophodiest_CpdA.
InterProiIPR013622. Calcineurin-like_phos_C.
IPR004843. Calcineurin-like_PHP_apaH.
IPR026575. cAMP_Pdiest_CpdA.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
ProDomiPD587589. Calcineurin-like_phos_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiCPDA_ECO57
AccessioniPrimary (citable) accession number: P0AEW5
Secondary accession number(s): P36650
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: January 7, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.