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P0AEW4

- CPDA_ECOLI

UniProt

P0AEW4 - CPDA_ECOLI

Protein

3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA

Gene

cpdA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Hydrolyzes cAMP to 5'-AMP. Plays an important regulatory role in modulating the intracellular concentration of cAMP, thereby influencing cAMP-dependent processes. Specific for cAMP.1 PublicationUniRule annotation

    Catalytic activityi

    Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.1 PublicationUniRule annotation

    Cofactori

    Binds 2 metal cations per subunit.UniRule annotation

    Enzyme regulationi

    Activated by iron.1 Publication

    Kineticsi

    1. KM=0.5 mM for cAMP1 Publication

    Vmax=2.0 µmol/min/mg enzyme1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi22 – 221Metal cation 1UniRule annotation
    Metal bindingi24 – 241Metal cation 1UniRule annotation
    Binding sitei24 – 241cAMPUniRule annotation
    Metal bindingi64 – 641Metal cation 1UniRule annotation
    Metal bindingi64 – 641Metal cation 2UniRule annotation
    Binding sitei64 – 641cAMPUniRule annotation
    Metal bindingi94 – 941Metal cation 2UniRule annotation
    Metal bindingi164 – 1641Metal cation 2UniRule annotation
    Metal bindingi203 – 2031Metal cation 2UniRule annotation
    Metal bindingi205 – 2051Metal cation 1UniRule annotation
    Binding sitei205 – 2051cAMPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi94 – 952cAMPUniRule annotation

    GO - Molecular functioni

    1. 3',5'-cyclic-AMP phosphodiesterase activity Source: EcoCyc
    2. ferrous iron binding Source: EcoCyc
    3. nucleotide binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. metabolic process Source: GOC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:G7579-MONOMER.
    ECOL316407:JW3000-MONOMER.
    MetaCyc:G7579-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3',5'-cyclic adenosine monophosphate phosphodiesterase CpdAUniRule annotation (EC:3.1.4.17UniRule annotation)
    Short name:
    3',5'-cyclic AMP phosphodiesteraseUniRule annotation
    Short name:
    cAMP phosphodiesteraseUniRule annotation
    Gene namesi
    Name:cpdAUniRule annotation
    Synonyms:icc
    Ordered Locus Names:b3032, JW3000
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG12187. cpdA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2752753',5'-cyclic adenosine monophosphate phosphodiesterase CpdAPRO_0000084146Add
    BLAST

    Proteomic databases

    PaxDbiP0AEW4.
    PRIDEiP0AEW4.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AEW4.

    Interactioni

    Protein-protein interaction databases

    IntActiP0AEW4. 10 interactions.
    STRINGi511145.b3032.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AEW4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cAMP phosphodiesterase class-III family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1409.
    HOGENOMiHOG000238351.
    KOiK03651.
    OMAiWREIELH.
    OrthoDBiEOG6QG8GQ.
    PhylomeDBiP0AEW4.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    HAMAPiMF_00905. cAMP_phophodiest_CpdA.
    InterProiIPR013622. Calcineurin-like_phos_C.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR026575. cAMP_Pdiest_CpdA.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    ProDomiPD587589. Calcineurin-like_phos_C. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF56300. SSF56300. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0AEW4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESLLTLPLA GEARVRILQI TDTHLFAQKH EALLGVNTWE SYQAVLEAIR    50
    PHQHEFDLIV ATGDLAQDQS SAAYQHFAEG IASFRAPCVW LPGNHDFQPA 100
    MYSALQDAGI SPAKRVFIGE QWQILLLDSQ VFGVPHGELS EFQLEWLERK 150
    LADAPERHTL LLLHHHPLPA GCSWLDQHSL RNAGELDTVL AKFPHVKYLL 200
    CGHIHQELDL DWNGRRLLAT PSTCVQFKPH CSNFTLDTIA PGWRTLELHA 250
    DGTLTTEVHR LADTRFQPDT ASEGY 275
    Length:275
    Mass (Da):30,938
    Last modified:December 20, 2005 - v1
    Checksum:i97696478536CFBF1
    GO

    Sequence cautioni

    The sequence AAA69200.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16557 Genomic DNA. Translation: BAA03989.1.
    U28377 Genomic DNA. Translation: AAA69200.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC76068.1.
    AP009048 Genomic DNA. Translation: BAE77088.1.
    PIRiF65090.
    RefSeqiNP_417504.1. NC_000913.3.
    YP_491224.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76068; AAC76068; b3032.
    BAE77088; BAE77088; BAE77088.
    GeneIDi12933401.
    947515.
    KEGGiecj:Y75_p2958.
    eco:b3032.
    PATRICi32121474. VBIEscCol129921_3124.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16557 Genomic DNA. Translation: BAA03989.1 .
    U28377 Genomic DNA. Translation: AAA69200.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC76068.1 .
    AP009048 Genomic DNA. Translation: BAE77088.1 .
    PIRi F65090.
    RefSeqi NP_417504.1. NC_000913.3.
    YP_491224.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AEW4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0AEW4. 10 interactions.
    STRINGi 511145.b3032.

    Proteomic databases

    PaxDbi P0AEW4.
    PRIDEi P0AEW4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76068 ; AAC76068 ; b3032 .
    BAE77088 ; BAE77088 ; BAE77088 .
    GeneIDi 12933401.
    947515.
    KEGGi ecj:Y75_p2958.
    eco:b3032.
    PATRICi 32121474. VBIEscCol129921_3124.

    Organism-specific databases

    EchoBASEi EB2104.
    EcoGenei EG12187. cpdA.

    Phylogenomic databases

    eggNOGi COG1409.
    HOGENOMi HOG000238351.
    KOi K03651.
    OMAi WREIELH.
    OrthoDBi EOG6QG8GQ.
    PhylomeDBi P0AEW4.

    Enzyme and pathway databases

    BioCyci EcoCyc:G7579-MONOMER.
    ECOL316407:JW3000-MONOMER.
    MetaCyc:G7579-MONOMER.

    Miscellaneous databases

    PROi P0AEW4.

    Gene expression databases

    Genevestigatori P0AEW4.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    HAMAPi MF_00905. cAMP_phophodiest_CpdA.
    InterProi IPR013622. Calcineurin-like_phos_C.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR026575. cAMP_Pdiest_CpdA.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    ProDomi PD587589. Calcineurin-like_phos_C. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification of the cpdA gene encoding cyclic 3',5'-adenosine monophosphate phosphodiesterase in Escherichia coli."
      Imamura R., Yamanaka K., Ogura T., Hiraga S., Fujita N., Ishihama A., Niki H.
      J. Biol. Chem. 271:25423-25429(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18, GENE NAME, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "3',5' Cyclic nucleotide phosphodiesterases class III: members, structure, and catalytic mechanism."
      Richter W.
      Proteins 46:278-286(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE, MODELING OF METAL-BINDING SITES.

    Entry informationi

    Entry nameiCPDA_ECOLI
    AccessioniPrimary (citable) accession number: P0AEW4
    Secondary accession number(s): P36650, Q2M9G8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3