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P0AEW4

- CPDA_ECOLI

UniProt

P0AEW4 - CPDA_ECOLI

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Protein

3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA

Gene
cpdA, icc, b3032, JW3000
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes cAMP to 5'-AMP. Plays an important regulatory role in modulating the intracellular concentration of cAMP, thereby influencing cAMP-dependent processes. Specific for cAMP.1 Publication

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.1 Publication

Cofactori

Binds 2 metal cations per subunit By similarity.UniRule annotation

Enzyme regulationi

Activated by iron.1 Publication

Kineticsi

  1. KM=0.5 mM for cAMP1 Publication

Vmax=2.0 µmol/min/mg enzyme

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi22 – 221Metal cation 1 By similarity
Metal bindingi24 – 241Metal cation 1 By similarity
Binding sitei24 – 241cAMP By similarity
Metal bindingi64 – 641Metal cation 1 By similarity
Metal bindingi64 – 641Metal cation 2 By similarity
Binding sitei64 – 641cAMP By similarity
Metal bindingi94 – 941Metal cation 2 By similarity
Metal bindingi164 – 1641Metal cation 2 By similarity
Metal bindingi203 – 2031Metal cation 2 By similarity
Metal bindingi205 – 2051Metal cation 1 By similarity
Binding sitei205 – 2051cAMP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi94 – 952cAMP By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: EcoCyc
  2. ferrous iron binding Source: EcoCyc
  3. nucleotide binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7579-MONOMER.
ECOL316407:JW3000-MONOMER.
MetaCyc:G7579-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA (EC:3.1.4.17)
Short name:
3',5'-cyclic AMP phosphodiesterase
Short name:
cAMP phosphodiesterase
Gene namesi
Name:cpdA
Synonyms:icc
Ordered Locus Names:b3032, JW3000
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12187. cpdA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2752753',5'-cyclic adenosine monophosphate phosphodiesterase CpdAUniRule annotationPRO_0000084146Add
BLAST

Proteomic databases

PaxDbiP0AEW4.
PRIDEiP0AEW4.

Expressioni

Gene expression databases

GenevestigatoriP0AEW4.

Interactioni

Protein-protein interaction databases

IntActiP0AEW4. 10 interactions.
STRINGi511145.b3032.

Structurei

3D structure databases

ProteinModelPortaliP0AEW4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000238351.
KOiK03651.
OMAiWREIELH.
OrthoDBiEOG6QG8GQ.
PhylomeDBiP0AEW4.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
HAMAPiMF_00905. cAMP_phophodiest_CpdA.
InterProiIPR013622. Calcineurin-like_phos_C.
IPR004843. Calcineurin-like_PHP_apaH.
IPR026575. cAMP_Pdiest_CpdA.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
ProDomiPD587589. Calcineurin-like_phos_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AEW4-1 [UniParc]FASTAAdd to Basket

« Hide

MESLLTLPLA GEARVRILQI TDTHLFAQKH EALLGVNTWE SYQAVLEAIR    50
PHQHEFDLIV ATGDLAQDQS SAAYQHFAEG IASFRAPCVW LPGNHDFQPA 100
MYSALQDAGI SPAKRVFIGE QWQILLLDSQ VFGVPHGELS EFQLEWLERK 150
LADAPERHTL LLLHHHPLPA GCSWLDQHSL RNAGELDTVL AKFPHVKYLL 200
CGHIHQELDL DWNGRRLLAT PSTCVQFKPH CSNFTLDTIA PGWRTLELHA 250
DGTLTTEVHR LADTRFQPDT ASEGY 275
Length:275
Mass (Da):30,938
Last modified:December 20, 2005 - v1
Checksum:i97696478536CFBF1
GO

Sequence cautioni

The sequence AAA69200.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16557 Genomic DNA. Translation: BAA03989.1.
U28377 Genomic DNA. Translation: AAA69200.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76068.1.
AP009048 Genomic DNA. Translation: BAE77088.1.
PIRiF65090.
RefSeqiNP_417504.1. NC_000913.3.
YP_491224.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76068; AAC76068; b3032.
BAE77088; BAE77088; BAE77088.
GeneIDi12933401.
947515.
KEGGiecj:Y75_p2958.
eco:b3032.
PATRICi32121474. VBIEscCol129921_3124.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16557 Genomic DNA. Translation: BAA03989.1 .
U28377 Genomic DNA. Translation: AAA69200.1 . Different initiation.
U00096 Genomic DNA. Translation: AAC76068.1 .
AP009048 Genomic DNA. Translation: BAE77088.1 .
PIRi F65090.
RefSeqi NP_417504.1. NC_000913.3.
YP_491224.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AEW4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P0AEW4. 10 interactions.
STRINGi 511145.b3032.

Proteomic databases

PaxDbi P0AEW4.
PRIDEi P0AEW4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76068 ; AAC76068 ; b3032 .
BAE77088 ; BAE77088 ; BAE77088 .
GeneIDi 12933401.
947515.
KEGGi ecj:Y75_p2958.
eco:b3032.
PATRICi 32121474. VBIEscCol129921_3124.

Organism-specific databases

EchoBASEi EB2104.
EcoGenei EG12187. cpdA.

Phylogenomic databases

eggNOGi COG1409.
HOGENOMi HOG000238351.
KOi K03651.
OMAi WREIELH.
OrthoDBi EOG6QG8GQ.
PhylomeDBi P0AEW4.

Enzyme and pathway databases

BioCyci EcoCyc:G7579-MONOMER.
ECOL316407:JW3000-MONOMER.
MetaCyc:G7579-MONOMER.

Miscellaneous databases

PROi P0AEW4.

Gene expression databases

Genevestigatori P0AEW4.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
HAMAPi MF_00905. cAMP_phophodiest_CpdA.
InterProi IPR013622. Calcineurin-like_phos_C.
IPR004843. Calcineurin-like_PHP_apaH.
IPR026575. cAMP_Pdiest_CpdA.
IPR029052. Metallo-depent_PP-like.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
ProDomi PD587589. Calcineurin-like_phos_C. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the cpdA gene encoding cyclic 3',5'-adenosine monophosphate phosphodiesterase in Escherichia coli."
    Imamura R., Yamanaka K., Ogura T., Hiraga S., Fujita N., Ishihama A., Niki H.
    J. Biol. Chem. 271:25423-25429(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18, GENE NAME, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "3',5' Cyclic nucleotide phosphodiesterases class III: members, structure, and catalytic mechanism."
    Richter W.
    Proteins 46:278-286(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, MODELING OF METAL-BINDING SITES.

Entry informationi

Entry nameiCPDA_ECOLI
AccessioniPrimary (citable) accession number: P0AEW4
Secondary accession number(s): P36650, Q2M9G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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