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Protein

Hydrogenase 3 maturation protease

Gene

hycI

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Protease involved in the C-terminal processing of HycE, the large subunit of hydrogenase 3.By similarity

Catalytic activityi

This enzyme specifically removes a 32-amino acid peptide from the C-terminus of the precursor of the large subunit of E.coli hydrogenase 3 by cleavage at the C-terminal side of Arg(537).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16NickelBy similarity1
Metal bindingi62NickelBy similarity1
Metal bindingi90NickelBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciECOO157:HYCI-MONOMER.

Protein family/group databases

MEROPSiA31.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase 3 maturation protease (EC:3.4.23.51)
Alternative name(s):
HycI protease
Gene namesi
Name:hycI
Ordered Locus Names:Z4025, ECs3573
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002019452 – 156Hydrogenase 3 maturation proteaseAdd BLAST155

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

MINTiMINT-1244218.
STRINGi155864.Z4025.

Structurei

3D structure databases

ProteinModelPortaliP0AEW0.
SMRiP0AEW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A31 family.Curated

Phylogenomic databases

eggNOGiENOG4108MG9. Bacteria.
COG0680. LUCA.
HOGENOMiHOG000278720.
KOiK08315.
OMAiVAFYFPI.

Family and domain databases

CDDicd06067. H2MP_MemB-H2evol. 1 hit.
Gene3Di3.40.50.1450. 1 hit.
InterProiIPR004420. Pept_A31_hyd_mat_HycI.
IPR023430. Pept_HybD-like_dom.
IPR000671. Peptidase_A31.
[Graphical view]
PfamiPF01750. HycI. 1 hit.
[Graphical view]
PRINTSiPR00446. HYDRGNUPTAKE.
SUPFAMiSSF53163. SSF53163. 1 hit.
TIGRFAMsiTIGR00142. hycI. 1 hit.
TIGR00072. hydrog_prot. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDVLLCVGN SMMGDDGAGP LLAEKCAAAP KGNWVVIDGG SAPENDIVAI
60 70 80 90 100
RELRPTRLLI VDATDMGLNP GEIRIIDPDD IAEMFMMTTH NMPLNYLIDQ
110 120 130 140 150
LKEDIGEVIF LGIQPDIVGF YYPMTQPIKD AVETVYQRLE GWEGNGGFAQ

LAVEEE
Length:156
Mass (Da):17,057
Last modified:January 23, 2007 - v2
Checksum:iCCE90535AFC0F30D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57824.1.
BA000007 Genomic DNA. Translation: BAB36996.1.
PIRiD85920.
E91075.
RefSeqiNP_311600.1. NC_002695.1.
WP_000132961.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG57824; AAG57824; Z4025.
BAB36996; BAB36996; BAB36996.
GeneIDi914705.
KEGGiece:Z4025.
ecs:ECs3573.
PATRICi18356613. VBIEscCol44059_3489.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57824.1.
BA000007 Genomic DNA. Translation: BAB36996.1.
PIRiD85920.
E91075.
RefSeqiNP_311600.1. NC_002695.1.
WP_000132961.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliP0AEW0.
SMRiP0AEW0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1244218.
STRINGi155864.Z4025.

Protein family/group databases

MEROPSiA31.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG57824; AAG57824; Z4025.
BAB36996; BAB36996; BAB36996.
GeneIDi914705.
KEGGiece:Z4025.
ecs:ECs3573.
PATRICi18356613. VBIEscCol44059_3489.

Phylogenomic databases

eggNOGiENOG4108MG9. Bacteria.
COG0680. LUCA.
HOGENOMiHOG000278720.
KOiK08315.
OMAiVAFYFPI.

Enzyme and pathway databases

BioCyciECOO157:HYCI-MONOMER.

Family and domain databases

CDDicd06067. H2MP_MemB-H2evol. 1 hit.
Gene3Di3.40.50.1450. 1 hit.
InterProiIPR004420. Pept_A31_hyd_mat_HycI.
IPR023430. Pept_HybD-like_dom.
IPR000671. Peptidase_A31.
[Graphical view]
PfamiPF01750. HycI. 1 hit.
[Graphical view]
PRINTSiPR00446. HYDRGNUPTAKE.
SUPFAMiSSF53163. SSF53163. 1 hit.
TIGRFAMsiTIGR00142. hycI. 1 hit.
TIGR00072. hydrog_prot. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHYCI_ECO57
AccessioniPrimary (citable) accession number: P0AEW0
Secondary accession number(s): Q57451
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.