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Protein

Hydrogenase 3 maturation protease

Gene

hycI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Protease involved in the C-terminal processing of HycE, the large subunit of hydrogenase 3.

Catalytic activityi

This enzyme specifically removes a 32-amino acid peptide from the C-terminus of the precursor of the large subunit of E.coli hydrogenase 3 by cleavage at the C-terminal side of Arg(537).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16NickelBy similarity1
Metal bindingi62NickelBy similarity1
Metal bindingi90NickelBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciEcoCyc:G7414-MONOMER.
ECOL316407:JW2687-MONOMER.
MetaCyc:G7414-MONOMER.
BRENDAi3.4.23.51. 2026.

Protein family/group databases

MEROPSiA31.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase 3 maturation protease (EC:3.4.23.51)
Alternative name(s):
HycI protease
Gene namesi
Name:hycI
Ordered Locus Names:b2717, JW2687
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13396. hycI.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi16D → N: No protease activity. 1 Publication1
Mutagenesisi62D → N or M: No protease activity. 1 Publication1
Mutagenesisi90H → Q: A little protease activity remaining. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002019442 – 156Hydrogenase 3 maturation proteaseAdd BLAST155

Proteomic databases

PaxDbiP0AEV9.
PRIDEiP0AEV9.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4261309. 13 interactors.
DIPiDIP-48019N.
IntActiP0AEV9. 9 interactors.
MINTiMINT-1244231.
STRINGi511145.b2717.

Structurei

Secondary structure

1156
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi11 – 17Combined sources7
Helixi18 – 28Combined sources11
Beta strandi35 – 38Combined sources4
Helixi44 – 46Combined sources3
Helixi47 – 53Combined sources7
Beta strandi56 – 63Combined sources8
Beta strandi73 – 75Combined sources3
Helixi78 – 83Combined sources6
Helixi85 – 88Combined sources4
Beta strandi89 – 91Combined sources3
Helixi94 – 105Combined sources12
Beta strandi106 – 113Combined sources8
Helixi126 – 136Combined sources11
Turni139 – 141Combined sources3
Turni144 – 147Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E85X-ray1.70A/B1-156[»]
2I8LNMR-A1-156[»]
ProteinModelPortaliP0AEV9.
SMRiP0AEV9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEV9.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A31 family.Curated

Phylogenomic databases

eggNOGiENOG4108MG9. Bacteria.
COG0680. LUCA.
HOGENOMiHOG000278720.
InParanoidiP0AEV9.
KOiK08315.
OMAiVAFYFPI.
PhylomeDBiP0AEV9.

Family and domain databases

CDDicd06067. H2MP_MemB-H2evol. 1 hit.
Gene3Di3.40.50.1450. 1 hit.
InterProiIPR004420. Pept_A31_hyd_mat_HycI.
IPR023430. Pept_HybD-like_dom.
IPR000671. Peptidase_A31.
[Graphical view]
PfamiPF01750. HycI. 1 hit.
[Graphical view]
PRINTSiPR00446. HYDRGNUPTAKE.
SUPFAMiSSF53163. SSF53163. 1 hit.
TIGRFAMsiTIGR00142. hycI. 1 hit.
TIGR00072. hydrog_prot. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDVLLCVGN SMMGDDGAGP LLAEKCAAAP KGNWVVIDGG SAPENDIVAI
60 70 80 90 100
RELRPTRLLI VDATDMGLNP GEIRIIDPDD IAEMFMMTTH NMPLNYLIDQ
110 120 130 140 150
LKEDIGEVIF LGIQPDIVGF YYPMTQPIKD AVETVYQRLE GWEGNGGFAQ

LAVEEE
Length:156
Mass (Da):17,057
Last modified:January 23, 2007 - v2
Checksum:iCCE90535AFC0F30D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA. Translation: CAA35554.1.
U29579 Genomic DNA. Translation: AAA69227.1.
U00096 Genomic DNA. Translation: AAC75759.1.
AP009048 Genomic DNA. Translation: BAE76794.1.
PIRiS67469.
RefSeqiNP_417197.1. NC_000913.3.
WP_000132961.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75759; AAC75759; b2717.
BAE76794; BAE76794; BAE76794.
GeneIDi947428.
KEGGiecj:JW2687.
eco:b2717.
PATRICi32120834. VBIEscCol129921_2809.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA. Translation: CAA35554.1.
U29579 Genomic DNA. Translation: AAA69227.1.
U00096 Genomic DNA. Translation: AAC75759.1.
AP009048 Genomic DNA. Translation: BAE76794.1.
PIRiS67469.
RefSeqiNP_417197.1. NC_000913.3.
WP_000132961.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E85X-ray1.70A/B1-156[»]
2I8LNMR-A1-156[»]
ProteinModelPortaliP0AEV9.
SMRiP0AEV9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261309. 13 interactors.
DIPiDIP-48019N.
IntActiP0AEV9. 9 interactors.
MINTiMINT-1244231.
STRINGi511145.b2717.

Protein family/group databases

MEROPSiA31.003.

Proteomic databases

PaxDbiP0AEV9.
PRIDEiP0AEV9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75759; AAC75759; b2717.
BAE76794; BAE76794; BAE76794.
GeneIDi947428.
KEGGiecj:JW2687.
eco:b2717.
PATRICi32120834. VBIEscCol129921_2809.

Organism-specific databases

EchoBASEiEB3177.
EcoGeneiEG13396. hycI.

Phylogenomic databases

eggNOGiENOG4108MG9. Bacteria.
COG0680. LUCA.
HOGENOMiHOG000278720.
InParanoidiP0AEV9.
KOiK08315.
OMAiVAFYFPI.
PhylomeDBiP0AEV9.

Enzyme and pathway databases

BioCyciEcoCyc:G7414-MONOMER.
ECOL316407:JW2687-MONOMER.
MetaCyc:G7414-MONOMER.
BRENDAi3.4.23.51. 2026.

Miscellaneous databases

EvolutionaryTraceiP0AEV9.
PROiP0AEV9.

Family and domain databases

CDDicd06067. H2MP_MemB-H2evol. 1 hit.
Gene3Di3.40.50.1450. 1 hit.
InterProiIPR004420. Pept_A31_hyd_mat_HycI.
IPR023430. Pept_HybD-like_dom.
IPR000671. Peptidase_A31.
[Graphical view]
PfamiPF01750. HycI. 1 hit.
[Graphical view]
PRINTSiPR00446. HYDRGNUPTAKE.
SUPFAMiSSF53163. SSF53163. 1 hit.
TIGRFAMsiTIGR00142. hycI. 1 hit.
TIGR00072. hydrog_prot. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHYCI_ECOLI
AccessioniPrimary (citable) accession number: P0AEV9
Secondary accession number(s): Q2MAB2, Q57451
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.