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Protein

Hydrogenase 3 maturation protease

Gene

hycI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protease involved in the C-terminal processing of HycE, the large subunit of hydrogenase 3.1 Publication

Catalytic activityi

This enzyme specifically removes a 32-amino acid peptide from the C-terminus of the precursor of the large subunit of E.coli hydrogenase 3 by cleavage at the C-terminal side of Arg-537.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16NickelBy similarity1
Metal bindingi62NickelBy similarity1
Metal bindingi90NickelBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
LigandMetal-binding, Nickel

Enzyme and pathway databases

BioCyciEcoCyc:G7414-MONOMER
MetaCyc:G7414-MONOMER
BRENDAi3.4.23.51 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase 3 maturation protease (EC:3.4.23.511 Publication)
Alternative name(s):
HycI protease
Gene namesi
Name:hycI
Ordered Locus Names:b2717, JW2687
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13396 hycI

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi16D → N: No protease activity. 1 Publication1
Mutagenesisi62D → N or M: No protease activity. 1 Publication1
Mutagenesisi90H → Q: A little protease activity remaining. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002019442 – 156Hydrogenase 3 maturation proteaseAdd BLAST155

Proteomic databases

PaxDbiP0AEV9
PRIDEiP0AEV9

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
aceEP0AFG82EBI-552628,EBI-542683

Protein-protein interaction databases

BioGridi4261309, 13 interactors
DIPiDIP-48019N
IntActiP0AEV9, 9 interactors
STRINGi316385.ECDH10B_2885

Structurei

Secondary structure

1156
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi11 – 17Combined sources7
Helixi18 – 28Combined sources11
Beta strandi35 – 38Combined sources4
Helixi44 – 46Combined sources3
Helixi47 – 53Combined sources7
Beta strandi56 – 63Combined sources8
Beta strandi73 – 75Combined sources3
Helixi78 – 83Combined sources6
Helixi85 – 88Combined sources4
Beta strandi89 – 91Combined sources3
Helixi94 – 105Combined sources12
Beta strandi106 – 113Combined sources8
Helixi126 – 136Combined sources11
Turni139 – 141Combined sources3
Turni144 – 147Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E85X-ray1.70A/B1-156[»]
2I8LNMR-A1-156[»]
ProteinModelPortaliP0AEV9
SMRiP0AEV9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEV9

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A31 family.Curated

Phylogenomic databases

eggNOGiENOG4108MG9 Bacteria
COG0680 LUCA
HOGENOMiHOG000278720
InParanoidiP0AEV9
KOiK08315
OMAiEMCAANP
PhylomeDBiP0AEV9

Family and domain databases

CDDicd06067 H2MP_MemB-H2evol, 1 hit
Gene3Di3.40.50.1450, 1 hit
InterProiView protein in InterPro
IPR004420 Pept_A31_hyd_mat_HycI
IPR023430 Pept_HybD-like_dom_sf
IPR000671 Peptidase_A31
PANTHERiPTHR30302 PTHR30302, 1 hit
PfamiView protein in Pfam
PF01750 HycI, 1 hit
PRINTSiPR00446 HYDRGNUPTAKE
SUPFAMiSSF53163 SSF53163, 1 hit
TIGRFAMsiTIGR00142 hycI, 1 hit
TIGR00072 hydrog_prot, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDVLLCVGN SMMGDDGAGP LLAEKCAAAP KGNWVVIDGG SAPENDIVAI
60 70 80 90 100
RELRPTRLLI VDATDMGLNP GEIRIIDPDD IAEMFMMTTH NMPLNYLIDQ
110 120 130 140 150
LKEDIGEVIF LGIQPDIVGF YYPMTQPIKD AVETVYQRLE GWEGNGGFAQ

LAVEEE
Length:156
Mass (Da):17,057
Last modified:January 23, 2007 - v2
Checksum:iCCE90535AFC0F30D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA Translation: CAA35554.1
U29579 Genomic DNA Translation: AAA69227.1
U00096 Genomic DNA Translation: AAC75759.1
AP009048 Genomic DNA Translation: BAE76794.1
PIRiS67469
RefSeqiNP_417197.1, NC_000913.3
WP_000132961.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75759; AAC75759; b2717
BAE76794; BAE76794; BAE76794
GeneIDi947428
KEGGiecj:JW2687
eco:b2717
PATRICifig|1411691.4.peg.4024

Similar proteinsi

Entry informationi

Entry nameiHYCI_ECOLI
AccessioniPrimary (citable) accession number: P0AEV9
Secondary accession number(s): Q2MAB2, Q57451
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 100 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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