Reviewed, UniProtKB/Swiss-Prot P0AEV9 (HYCI_ECOLI)

Names and origin

Protein names

Recommended name:
    Hydrogenase 3 maturation protease
    EC=3.4.23.-

Gene names

Name:	hycI
Ordered Locus Names:	b2717, JW2687

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Protease involved in the C-terminal processing of hycE, the large subunit of hydrogenase 3.
Subunit structure	Monomer.
Sequence similarities	Belongs to the peptidase A31 family.

Ontologies

Keywords
Ligand	Metal-binding Nickel
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW enzyme activator activity Inferred from electronic annotation. Source: InterPro nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
aceE	P0AFG8	1	EBI-552628,EBI-542683

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 156

155

Hydrogenase 3 maturation protease

PRO_0000201944

Sites

Metal binding

16

1

Nickel By similarity

Metal binding

62

1

Nickel By similarity

Metal binding

90

1

Nickel By similarity

Secondary structure

1

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156

Helix Strand Turn

Details...

Beta strand

3 – 8

6

Helix

11 – 17

7

Helix

18 – 28

11

Beta strand

35 – 38

4

Helix

44 – 46

3

Helix

47 – 53

7

Beta strand

56 – 63

8

Beta strand

73 – 75

3

Helix

78 – 83

6

Helix

94 – 105

12

Beta strand

106 – 113

8

Helix

126 – 136

11

Turn

144 – 147

4

Sequences

	Sequence		Length	Mass (Da)
	P0AEV9-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: CCE90535AFC0F30D	FASTA	156	17,057
	10 20 30 40 50 60 MTDVLLCVGN SMMGDDGAGP LLAEKCAAAP KGNWVVIDGG SAPENDIVAI RELRPTRLLI 70 80 90 100 110 120 VDATDMGLNP GEIRIIDPDD IAEMFMMTTH NMPLNYLIDQ LKEDIGEVIF LGIQPDIVGF 130 140 150 YYPMTQPIKD AVETVYQRLE GWEGNGGFAQ LAVEEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterisation of a protease from Escherichia coli involved in hydrogenase maturation." Rossmann R., Maier T., Lottspeich F., Boeck A. Eur. J. Biochem. 227:545-550(1995) [PubMed: 7851435] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-37 AND 66-84. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X17506 Genomic DNA. Translation: CAA35554.1.
U29579 Genomic DNA. Translation: AAA69227.1.
U00096 Genomic DNA. Translation: AAC75759.1.
AP009048 Genomic DNA. Translation: BAE76794.1.

PIR

S67469.

RefSeq

AP_003284.1.
NP_417197.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2E85	X-ray	1.70	A/B	2-156	[»]
2I8L	NMR	-	A	1-156	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0AEV9. 9 interactions.

Genome annotation databases

GeneID

947428.

GenomeReviews

Gene locus JW2687 in contig AP009048_GR.
Gene locus b2717 in contig U00096_GR.

KEGG

ecj:JW2687.
eco:b2717.

Organism-specific databases

EchoBASE

EB3177.

EcoGene

EG13396. hycI.

CMR

Search...

Phylogenomic databases

HOGENOM

P0AEV9.

OMA

P0AEV9. MPLNYLV.

Enzyme and pathway databases

BioCyc

EcoCyc:G7414-MON.

Family and domain databases

InterPro

IPR004420. Pept_A31_hyd_mat_HycI.
IPR000671. Peptidase_A31.
[Graphical view]

Gene3D

G3DSA:3.40.50.1450. Peptidase_A31. 1 hit.

Pfam

PF01750. HycI. 1 hit.
[Graphical view]

PRINTS

PR00446. HYDRGNUPTAKE.

TIGRFAMs

TIGR00142. hycI. 1 hit.
TIGR00072. hydrog_prot. 1 hit.

ProtoNet

Search...

Entry information

Entry name

HYCI_ECOLI

Accession

Primary (citable) accession number: P0AEV9
Secondary accession number(s): Q2MAB2, Q57451

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)