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P0AEV1

- RSSB_ECOLI

UniProt

P0AEV1 - RSSB_ECOLI

Protein

Regulator of RpoS

Gene

rssB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Regulates the turnover of the sigma S factor (RpoS) by promoting its proteolysis in exponentially growing cells. Acts by binding and delivering RpoS to the ClpXP protease. RssB is not co-degraded with RpoS, but is released from the complex and can initiate a new cycle of RpoS recognition and degradation. In stationary phase, could also act as an anti-sigma factor and reduce the ability of RpoS to activate gene expression. Is also involved in the regulation of the mRNA polyadenylation pathway during stationary phase, probably by maintaining the association of PcnB with the degradosome.6 PublicationsUniRule annotation

    Enzyme regulationi

    Under certain stress conditions, activity is inhibited by the anti-adapter proteins IraP, IraD and IraM. IraP is involved in response to phosphate stavation, IraD in response to DNA damage and IraM in response to magnesium starvation. IraD and IraM interact with inactive RssB, blocking its ability to interact with RpoS. IraP may mimic RpoS in its interaction with RssB and directly competing with RpoS for binding to RssB.3 Publications

    GO - Molecular functioni

    1. phosphorelay response regulator activity Source: InterPro
    2. protein binding Source: EcoCyc
    3. sigma factor antagonist activity Source: EcoCyc

    GO - Biological processi

    1. positive regulation of proteolysis Source: EcoCyc
    2. protein destabilization Source: EcoCyc
    3. regulation of RNA biosynthetic process Source: GOC
    4. response to stress Source: UniProtKB-KW

    Keywords - Biological processi

    Stress response

    Enzyme and pathway databases

    BioCyciEcoCyc:EG12121-MONOMER.
    ECOL316407:JW1223-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulator of RpoSUniRule annotation
    Gene namesi
    Name:rssBUniRule annotation
    Synonyms:hnr, sprE, ychL
    Ordered Locus Names:b1235, JW1223
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG12121. rssB.

    Pathology & Biotechi

    Disruption phenotypei

    Mutants exhibit nearly constitutively high levels of RpoS and are impaired in the post-transcriptional growth phase-related and osmotic regulation of RpoS. In exponentially growing cells, mutants exhibit significantly reduced levels of polyadenylation and increased stability of specific mRNAs.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi58 – 581D → P, Q or R: Lack of phosphorylation. Cannot bind RpoS. 3 Publications
    Mutagenesisi67 – 671L → A: Lack of phosphorylation. 1 Publication
    Mutagenesisi109 – 1091P → S: Resistant to IraP but not to IraD. Increases stabilization by IraM. Decreases phosphorylation. 1 Publication
    Mutagenesisi143 – 1431W → R: Resistant to both IraP and IraD. Increases stabilization by IraM. 1 Publication
    Mutagenesisi214 – 2141L → H: Resistant to IraP and IraD. 1 Publication
    Mutagenesisi216 – 2161A → T: Resistant to IraP, IraD and IraM. 1 Publication
    Mutagenesisi218 – 2181L → V: Resistant to IraP, IraD and IraM. 1 Publication
    Mutagenesisi255 – 2551A → V: Resistant to IraM. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 337337Regulator of RpoSPRO_0000081388Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei58 – 5814-aspartylphosphate1 PublicationUniRule annotation

    Post-translational modificationi

    Phosphorylated. Phosphorylation stimulates the interaction with RpoS and, therefore, the proteolysis of RpoS.4 PublicationsUniRule annotation

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP0AEV1.

    Expressioni

    Inductioni

    Expression is induced during stationary phase by RpoS.1 Publication

    Gene expression databases

    GenevestigatoriP0AEV1.

    Interactioni

    Subunit structurei

    Binds to RpoS with a stoichiometry of 1:1. Interacts with the anti-adapter proteins IraP, IraD and IraM.6 PublicationsUniRule annotation

    Protein-protein interaction databases

    IntActiP0AEV1. 4 interactions.
    STRINGi511145.b1235.

    Structurei

    Secondary structure

    1
    337
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni4 – 74
    Beta strandi9 – 135
    Helixi17 – 2913
    Beta strandi33 – 386
    Helixi40 – 478
    Beta strandi53 – 575
    Helixi67 – 759
    Beta strandi82 – 865
    Helixi91 – 10010
    Beta strandi103 – 1086
    Helixi115 – 12410

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3EODX-ray1.75A1-130[»]
    ProteinModelPortaliP0AEV1.
    SMRiP0AEV1. Positions 3-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AEV1.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini9 – 123115Response regulatoryUniRule annotationAdd
    BLAST

    Domaini

    Contains an N-terminal receiver domain and a C-terminal output domain that are both required for binding to RpoS. IraP and IraD interact with the N-terminal domain. IraM interacts with the C-terminal domain and, more weakly, the N-terminal domain.2 Publications

    Sequence similaritiesi

    Belongs to the RssB family.UniRule annotation
    Contains 1 response regulatory domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0784.
    HOGENOMiHOG000119811.
    KOiK02485.
    OMAiATSEPIH.
    OrthoDBiEOG69GZGV.
    PhylomeDBiP0AEV1.

    Family and domain databases

    HAMAPiMF_00958. RssB.
    InterProiIPR011006. CheY-like_superfamily.
    IPR028616. RssB.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view]
    PfamiPF00072. Response_reg. 1 hit.
    [Graphical view]
    SMARTiSM00448. REC. 1 hit.
    [Graphical view]
    SUPFAMiSSF52172. SSF52172. 1 hit.
    PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AEV1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQPLVGKQI LIVEDEQVFR SLLDSWFSSL GATTVLAADG VDALELLGGF    50
    TPDLMICDIA MPRMNGLKLL EHIRNRGDQT PVLVISATEN MADIAKALRL 100
    GVEDVLLKPV KDLNRLREMV FACLYPSMFN SRVEEEERLF RDWDAMVDNP 150
    AAAAKLLQEL QPPVQQVISH CRVNYRQLVA ADKPGLVLDI AALSENDLAF 200
    YCLDVTRAGH NGVLAALLLR ALFNGLLQEQ LAHQNQRLPE LGALLKQVNH 250
    LLRQANLPGQ FPLLVGYYHR ELKNLILVSA GLNATLNTGE HQVQISNGVP 300
    LGTLGNAYLN QLSQRCDAWQ CQIWGTGGRL RLMLSAE 337
    Length:337
    Mass (Da):37,302
    Last modified:December 20, 2005 - v1
    Checksum:iAB962EF94BC7B470
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66003 Genomic DNA. Translation: CAA46802.1.
    M64675 Unassigned DNA. No translation available.
    U00096 Genomic DNA. Translation: AAC74317.1.
    AP009048 Genomic DNA. Translation: BAA36103.1.
    PIRiA36871.
    RefSeqiNP_415751.1. NC_000913.3.
    YP_489503.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74317; AAC74317; b1235.
    BAA36103; BAA36103; BAA36103.
    GeneIDi12932932.
    945855.
    KEGGiecj:Y75_p1208.
    eco:b1235.
    PATRICi32117726. VBIEscCol129921_1283.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66003 Genomic DNA. Translation: CAA46802.1 .
    M64675 Unassigned DNA. No translation available.
    U00096 Genomic DNA. Translation: AAC74317.1 .
    AP009048 Genomic DNA. Translation: BAA36103.1 .
    PIRi A36871.
    RefSeqi NP_415751.1. NC_000913.3.
    YP_489503.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3EOD X-ray 1.75 A 1-130 [» ]
    ProteinModelPortali P0AEV1.
    SMRi P0AEV1. Positions 3-282.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0AEV1. 4 interactions.
    STRINGi 511145.b1235.

    Proteomic databases

    PRIDEi P0AEV1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74317 ; AAC74317 ; b1235 .
    BAA36103 ; BAA36103 ; BAA36103 .
    GeneIDi 12932932.
    945855.
    KEGGi ecj:Y75_p1208.
    eco:b1235.
    PATRICi 32117726. VBIEscCol129921_1283.

    Organism-specific databases

    EchoBASEi EB2042.
    EcoGenei EG12121. rssB.

    Phylogenomic databases

    eggNOGi COG0784.
    HOGENOMi HOG000119811.
    KOi K02485.
    OMAi ATSEPIH.
    OrthoDBi EOG69GZGV.
    PhylomeDBi P0AEV1.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG12121-MONOMER.
    ECOL316407:JW1223-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0AEV1.
    PROi P0AEV1.

    Gene expression databases

    Genevestigatori P0AEV1.

    Family and domain databases

    HAMAPi MF_00958. RssB.
    InterProi IPR011006. CheY-like_superfamily.
    IPR028616. RssB.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view ]
    Pfami PF00072. Response_reg. 1 hit.
    [Graphical view ]
    SMARTi SM00448. REC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52172. SSF52172. 1 hit.
    PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Contreras A.
      Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Organization and functions of genes in the upstream region of tyrT of Escherichia coli: phenotypes of mutants with partial deletion of a new gene (tgs)."
      Boesl M., Kersten H.
      J. Bacteriol. 176:221-231(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "The response regulator RssB controls stability of the sigma(S) subunit of RNA polymerase in Escherichia coli."
      Muffler A., Fischer D., Altuvia S., Storz G., Hengge-Aronis R.
      EMBO J. 15:1333-1339(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, GENE NAME.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    7. "The response regulator SprE controls the stability of RpoS."
      Pratt L.A., Silhavy T.J.
      Proc. Natl. Acad. Sci. U.S.A. 93:2488-2492(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    8. "Regulation of RssB-dependent proteolysis in Escherichia coli: a role for acetyl phosphate in a response regulator-controlled process."
      Bouche S., Klauck E., Fischer D., Lucassen M., Jung K., Hengge-Aronis R.
      Mol. Microbiol. 27:787-795(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT ASP-58, MUTAGENESIS OF ASP-58.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    9. "Regulation of RpoS proteolysis in Escherichia coli: the response regulator RssB is a recognition factor that interacts with the turnover element in RpoS."
      Becker G., Klauck E., Hengge-Aronis R.
      Proc. Natl. Acad. Sci. U.S.A. 96:6439-6444(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RPOS, PHOSPHORYLATION.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    10. "SprE levels are growth phase regulated in a sigma(S)-dependent manner at the level of translation."
      Gibson K.E., Silhavy T.J.
      J. Bacteriol. 182:4117-4120(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    11. "The response regulator RssB, a recognition factor for sigmaS proteolysis in Escherichia coli, can act like an anti-sigmaS factor."
      Becker G., Klauck E., Hengge-Aronis R.
      Mol. Microbiol. 35:657-666(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS AN ANTI-SIGMA FACTOR, INTERACTION WITH RPOS.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    12. "Role of the response regulator RssB in sigma recognition and initiation of sigma proteolysis in Escherichia coli."
      Klauck E., Lingnau M., Hengge-Aronis R.
      Mol. Microbiol. 40:1381-1390(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RPOS, DOMAIN, PHOSPHORYLATION, MUTAGENESIS OF ASP-58.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    13. "Modulating RssB activity: IraP, a novel regulator of sigma(S) stability in Escherichia coli."
      Bougdour A., Wickner S., Gottesman S.
      Genes Dev. 20:884-897(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION, INTERACTION WITH IRAP.
      Strain: K12 / MG1655 / ATCC 47076.
    14. "Multiple pathways for regulation of sigmaS (RpoS) stability in Escherichia coli via the action of multiple anti-adaptors."
      Bougdour A., Cunning C., Baptiste P.J., Elliott T., Gottesman S.
      Mol. Microbiol. 68:298-313(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION, INTERACTION WITH IRAD AND IRAM.
      Strain: K12 / MG1655 / ATCC 47076.
    15. "The response regulator SprE (RssB) modulates polyadenylation and mRNA stability in Escherichia coli."
      Carabetta V.J., Mohanty B.K., Kushner S.R., Silhavy T.J.
      J. Bacteriol. 191:6812-6821(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN POLYADENYLATION, DISRUPTION PHENOTYPE.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    16. "The response regulator SprE (RssB) is required for maintaining poly(A) polymerase I-degradosome association during stationary phase."
      Carabetta V.J., Silhavy T.J., Cristea I.M.
      J. Bacteriol. 192:3713-3721(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN POLYADENYLATION.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    17. "Anti-adaptors provide multiple modes for regulation of the RssB adaptor protein."
      Battesti A., Hoskins J.R., Tong S., Milanesio P., Mann J.M., Kravats A., Tsegaye Y.M., Bougdour A., Wickner S., Gottesman S.
      Genes Dev. 27:2722-2735(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION, INTERACTION WITH IRAP; IRAD AND IRAM, DOMAIN, PHOSPHORYLATION, MUTAGENESIS OF ASP-58; LEU-67; PRO-109; TRP-143; LEU-214; ALA-216; LEU-218 AND ALA-255.
    18. "The structure of RssB, a ClpX adaptor protein that regulates sigma S."
      Levchenko I., Grant R.A., Sauer R.T., Baker T.A.
      Submitted (SEP-2008) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 1-130.

    Entry informationi

    Entry nameiRSSB_ECOLI
    AccessioniPrimary (citable) accession number: P0AEV1
    Secondary accession number(s): P37055
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3