P0AEU3 (HISM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histidine transport system permease protein hisM

Gene names

Name:	hisM
Ordered Locus Names:	b2307, JW2304

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the binding-protein-dependent transport system for histidine; probably responsible for the translocation of the substrate across the membrane.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. HisMQ subfamily. Contains 1 ABC transmembrane type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Histidine transport system permease protein hisM		PRO_0000060044
Regions
	Topological domain	1 – 26	26	Periplasmic Potential
	Transmembrane	27 – 47	21	Helical; Potential
	Topological domain	48 – 58	11	Cytoplasmic Potential
	Transmembrane	59 – 79	21	Helical; Potential
	Topological domain	80 – 104	25	Periplasmic Potential
	Transmembrane	105 – 125	21	Helical; Potential
	Topological domain	126 – 157	32	Cytoplasmic Potential
	Transmembrane	158 – 178	21	Helical; Potential
	Topological domain	179 – 200	22	Periplasmic Potential
	Transmembrane	201 – 221	21	Helical; Potential
	Topological domain	222 – 238	17	Cytoplasmic Potential
	Domain	23 – 221	199	ABC transmembrane type-1

Sequences

	Sequence		Length	Mass (Da)
	P0AEU3 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 0307840A94836348	FASTA	238	26,870
	10 20 30 40 50 60 MIEILHEYWK PLLWTDGYRF TGVAITLWLL ILSVVIGGVL ALFLAIGRVS SNKYIQFPIW 70 80 90 100 110 120 LFTYIFRGTP LYVQLLVFYS GMYTLEIVKG TEFLNAFFRS GLNCTVLALT LNTCAYTTEI 130 140 150 160 170 180 FAGAIRSVPH GEIEAARAYG FSTFKMYRCI ILPSALRIAL PAYSNEVILM LHSTALAFTA 190 200 210 220 230 TVPDLLKIAR DINAATYQPF TAFGIAAVLY LIISYVLISL FRRAEKRWLQ HVKPSSTH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	Joshi A., Ames G.F.-L. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-93. Strain: K12.
[5]	"Sequence of the complete P protein gene and part of the M protein gene from the histidine transport operon of Escherichia coli compared to that of Salmonella typhimurium." Kraft R., Leinwand L.A. Nucleic Acids Res. 15:8568-8568(1987) [PubMed: 3313284] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 92-238. Strain: K12.
[6]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC75367.1. AP009048 Genomic DNA. Translation: BAA16153.1. U47027 Genomic DNA. Translation: AAA85771.1. Y00455 Genomic DNA. Translation: CAA68510.1.
PIR	B27835. A65003.
RefSeq	NP_416810.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AEU3.
SMR	P0AEU3. Positions 18-219.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002497; EBESCP00000002497; EBESCG00000002037. EBESCT00000002498; EBESCP00000002498; EBESCG00000002037. EBESCT00000017082; EBESCP00000016373; EBESCG00000016141.
GeneID	946790.
GenomeReviews	Gene locus JW2304 in contig AP009048_GR. Gene locus b2307 in contig U00096_GR.
KEGG	ecj:JW2304. eco:b2307.
PATRIC	32119985. VBIEscCol129921_2402.
Organism-specific databases
EchoBASE	EB0007.
EcoGene	EG10007. hisM.
Phylogenomic databases
eggNOG	COG4160.
GeneTree	EBGT00050000008884.
HOGENOM	HBG704821.
OMA	QPFYAFG.
PhylomeDB	P0AEU3.
ProtClustDB	PRK15069.
Enzyme and pathway databases
BioCyc	EcoCyc:HISM-MONOMER.
Gene expression databases
Genevestigator	P0AEU3.
Family and domain databases
InterPro	IPR010065. AA_ABC_transptr_permease_3TM. IPR000515. BPD_transp. [Graphical view]
KO	K10015.
Pfam	PF00528. BPD_transp_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01726. HEQRo_perm_3TM. 1 hit.
PROSITE	PS50928. ABC_TM1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HISM_ECOLI

Accession

Primary (citable) accession number: P0AEU3
Secondary accession number(s): P20091, P76936

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program