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Protein

Histidine-binding periplasmic protein

Gene

hisJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the histidine permease ABC transporter. Binds histidine. Interacts with HisQMP and stimulates ATPase activity of HisP, which results in histidine translocation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:HISJ-MONOMER.
ECOL316407:JW2306-MONOMER.

Protein family/group databases

TCDBi3.A.1.3.29. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine-binding periplasmic protein
Short name:
HBP
Gene namesi
Name:hisJ
Ordered Locus Names:b2309, JW2306
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12124. hisJ.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 222 PublicationsAdd BLAST22
ChainiPRO_000003176223 – 260Histidine-binding periplasmic proteinAdd BLAST238

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi60 ↔ 67By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP0AEU0.
PaxDbiP0AEU0.
PRIDEiP0AEU0.

2D gel databases

SWISS-2DPAGEP0AEU0.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (HisP), two transmembrane proteins (HisM and HisQ) and a solute-binding protein (HisJ).By similarity

Protein-protein interaction databases

BioGridi4260524. 10 interactors.
DIPiDIP-9908N.
IntActiP0AEU0. 7 interactors.
STRINGi511145.b2309.

Structurei

Secondary structure

1260
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi29 – 32Combined sources4
Turni37 – 39Combined sources3
Beta strandi40 – 42Combined sources3
Beta strandi48 – 50Combined sources3
Helixi51 – 62Combined sources12
Beta strandi68 – 71Combined sources4
Helixi74 – 82Combined sources9
Beta strandi87 – 89Combined sources3
Helixi97 – 100Combined sources4
Beta strandi103 – 105Combined sources3
Beta strandi114 – 121Combined sources8
Helixi128 – 131Combined sources4
Beta strandi135 – 139Combined sources5
Helixi143 – 151Combined sources9
Helixi153 – 155Combined sources3
Beta strandi158 – 164Combined sources7
Helixi165 – 173Combined sources9
Beta strandi176 – 183Combined sources8
Helixi184 – 190Combined sources7
Turni191 – 193Combined sources3
Helixi195 – 199Combined sources5
Beta strandi200 – 205Combined sources6
Turni210 – 212Combined sources3
Beta strandi215 – 217Combined sources3
Helixi226 – 241Combined sources16
Helixi244 – 250Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HSLX-ray1.89A/B28-260[»]
ProteinModelPortaliP0AEU0.
SMRiP0AEU0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEU0.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105E89. Bacteria.
COG0834. LUCA.
HOGENOMiHOG000031896.
InParanoidiP0AEU0.
KOiK10014.
OMAiIPYQNQD.
PhylomeDBiP0AEU0.

Family and domain databases

InterProiIPR005768. Lys_Arg_Orn-bd.
IPR018313. SBP_3_CS.
IPR001638. Solute-binding_3/MltF_N.
[Graphical view]
PfamiPF00497. SBP_bac_3. 1 hit.
[Graphical view]
SMARTiSM00062. PBPb. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01096. 3A0103s03R. 1 hit.
PROSITEiPS01039. SBP_BACTERIAL_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLVLSLSL VLAFSSATAA FAAIPQNIRI GTDPTYAPFE SKNSQGELVG
60 70 80 90 100
FDIDLAKELC KRINTQCTFV ENPLDALIPS LKAKKIDAIM SSLSITEKRQ
110 120 130 140 150
QEIAFTDKLY AADSRLVVAK NSDIQPTVES LKGKRVGVLQ GTTQETFGNE
160 170 180 190 200
HWAPKGIEIV SYQGQDNIYS DLTAGRIDAA FQDEVAASEG FLKQPVGKDY
210 220 230 240 250
KFGGPSVKDE KLFGVGTGMG LRKEDNELRE ALNKAFAEMR ADGTYEKLAK
260
KYFDFDVYGG
Length:260
Mass (Da):28,483
Last modified:December 20, 2005 - v1
Checksum:i28BFFD0C67ABF716
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21F → L in AAA85769 (Ref. 1) Curated1
Sequence conflicti188S → G in AAA85769 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47027 Genomic DNA. Translation: AAA85769.1.
U00096 Genomic DNA. Translation: AAC75369.1.
AP009048 Genomic DNA. Translation: BAA16155.1.
PIRiC65003.
RefSeqiNP_416812.1. NC_000913.3.
WP_000737621.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75369; AAC75369; b2309.
BAA16155; BAA16155; BAA16155.
GeneIDi945309.
KEGGiecj:JW2306.
eco:b2309.
PATRICi32119989. VBIEscCol129921_2404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47027 Genomic DNA. Translation: AAA85769.1.
U00096 Genomic DNA. Translation: AAC75369.1.
AP009048 Genomic DNA. Translation: BAA16155.1.
PIRiC65003.
RefSeqiNP_416812.1. NC_000913.3.
WP_000737621.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HSLX-ray1.89A/B28-260[»]
ProteinModelPortaliP0AEU0.
SMRiP0AEU0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260524. 10 interactors.
DIPiDIP-9908N.
IntActiP0AEU0. 7 interactors.
STRINGi511145.b2309.

Protein family/group databases

TCDBi3.A.1.3.29. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP0AEU0.

Proteomic databases

EPDiP0AEU0.
PaxDbiP0AEU0.
PRIDEiP0AEU0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75369; AAC75369; b2309.
BAA16155; BAA16155; BAA16155.
GeneIDi945309.
KEGGiecj:JW2306.
eco:b2309.
PATRICi32119989. VBIEscCol129921_2404.

Organism-specific databases

EchoBASEiEB2045.
EcoGeneiEG12124. hisJ.

Phylogenomic databases

eggNOGiENOG4105E89. Bacteria.
COG0834. LUCA.
HOGENOMiHOG000031896.
InParanoidiP0AEU0.
KOiK10014.
OMAiIPYQNQD.
PhylomeDBiP0AEU0.

Enzyme and pathway databases

BioCyciEcoCyc:HISJ-MONOMER.
ECOL316407:JW2306-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AEU0.
PROiP0AEU0.

Family and domain databases

InterProiIPR005768. Lys_Arg_Orn-bd.
IPR018313. SBP_3_CS.
IPR001638. Solute-binding_3/MltF_N.
[Graphical view]
PfamiPF00497. SBP_bac_3. 1 hit.
[Graphical view]
SMARTiSM00062. PBPb. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01096. 3A0103s03R. 1 hit.
PROSITEiPS01039. SBP_BACTERIAL_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHISJ_ECOLI
AccessioniPrimary (citable) accession number: P0AEU0
Secondary accession number(s): P39182, P77763
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.