Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Histidine-binding periplasmic protein

Gene

hisJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the histidine permease ABC transporter. Binds histidine. Interacts with HisQMP and stimulates ATPase activity of HisP, which results in histidine translocation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:HISJ-MONOMER.
ECOL316407:JW2306-MONOMER.
MetaCyc:HISJ-MONOMER.
RETL1328306-WGS:GSTH-6770-MONOMER.

Protein family/group databases

TCDBi3.A.1.3.29. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine-binding periplasmic protein
Short name:
HBP
Gene namesi
Name:hisJ
Ordered Locus Names:b2309, JW2306
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12124. hisJ.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22222 PublicationsAdd
BLAST
Chaini23 – 260238Histidine-binding periplasmic proteinPRO_0000031762Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi60 ↔ 67By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP0AEU0.
PaxDbiP0AEU0.
PRIDEiP0AEU0.

2D gel databases

SWISS-2DPAGEP0AEU0.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (HisP), two transmembrane proteins (HisM and HisQ) and a solute-binding protein (HisJ).By similarity

Protein-protein interaction databases

BioGridi4260524. 10 interactions.
DIPiDIP-9908N.
IntActiP0AEU0. 7 interactions.
STRINGi511145.b2309.

Structurei

Secondary structure

1
260
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi29 – 324Combined sources
Turni37 – 393Combined sources
Beta strandi40 – 423Combined sources
Beta strandi48 – 503Combined sources
Helixi51 – 6212Combined sources
Beta strandi68 – 714Combined sources
Helixi74 – 829Combined sources
Beta strandi87 – 893Combined sources
Helixi97 – 1004Combined sources
Beta strandi103 – 1053Combined sources
Beta strandi114 – 1218Combined sources
Helixi128 – 1314Combined sources
Beta strandi135 – 1395Combined sources
Helixi143 – 1519Combined sources
Helixi153 – 1553Combined sources
Beta strandi158 – 1647Combined sources
Helixi165 – 1739Combined sources
Beta strandi176 – 1838Combined sources
Helixi184 – 1907Combined sources
Turni191 – 1933Combined sources
Helixi195 – 1995Combined sources
Beta strandi200 – 2056Combined sources
Turni210 – 2123Combined sources
Beta strandi215 – 2173Combined sources
Helixi226 – 24116Combined sources
Helixi244 – 2507Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HSLX-ray1.89A/B28-260[»]
ProteinModelPortaliP0AEU0.
SMRiP0AEU0. Positions 23-260.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEU0.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105E89. Bacteria.
COG0834. LUCA.
HOGENOMiHOG000031896.
InParanoidiP0AEU0.
KOiK10014.
OMAiIPYQNQD.
PhylomeDBiP0AEU0.

Family and domain databases

InterProiIPR005768. Lys_Arg_Orn-bd.
IPR018313. SBP_3_CS.
IPR001638. Solute-binding_3/MltF_N.
[Graphical view]
PfamiPF00497. SBP_bac_3. 1 hit.
[Graphical view]
SMARTiSM00062. PBPb. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01096. 3A0103s03R. 1 hit.
PROSITEiPS01039. SBP_BACTERIAL_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLVLSLSL VLAFSSATAA FAAIPQNIRI GTDPTYAPFE SKNSQGELVG
60 70 80 90 100
FDIDLAKELC KRINTQCTFV ENPLDALIPS LKAKKIDAIM SSLSITEKRQ
110 120 130 140 150
QEIAFTDKLY AADSRLVVAK NSDIQPTVES LKGKRVGVLQ GTTQETFGNE
160 170 180 190 200
HWAPKGIEIV SYQGQDNIYS DLTAGRIDAA FQDEVAASEG FLKQPVGKDY
210 220 230 240 250
KFGGPSVKDE KLFGVGTGMG LRKEDNELRE ALNKAFAEMR ADGTYEKLAK
260
KYFDFDVYGG
Length:260
Mass (Da):28,483
Last modified:December 20, 2005 - v1
Checksum:i28BFFD0C67ABF716
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti21 – 211F → L in AAA85769 (Ref. 1) Curated
Sequence conflicti188 – 1881S → G in AAA85769 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47027 Genomic DNA. Translation: AAA85769.1.
U00096 Genomic DNA. Translation: AAC75369.1.
AP009048 Genomic DNA. Translation: BAA16155.1.
PIRiC65003.
RefSeqiNP_416812.1. NC_000913.3.
WP_000737621.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75369; AAC75369; b2309.
BAA16155; BAA16155; BAA16155.
GeneIDi945309.
KEGGiecj:JW2306.
eco:b2309.
PATRICi32119989. VBIEscCol129921_2404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U47027 Genomic DNA. Translation: AAA85769.1.
U00096 Genomic DNA. Translation: AAC75369.1.
AP009048 Genomic DNA. Translation: BAA16155.1.
PIRiC65003.
RefSeqiNP_416812.1. NC_000913.3.
WP_000737621.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HSLX-ray1.89A/B28-260[»]
ProteinModelPortaliP0AEU0.
SMRiP0AEU0. Positions 23-260.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260524. 10 interactions.
DIPiDIP-9908N.
IntActiP0AEU0. 7 interactions.
STRINGi511145.b2309.

Protein family/group databases

TCDBi3.A.1.3.29. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP0AEU0.

Proteomic databases

EPDiP0AEU0.
PaxDbiP0AEU0.
PRIDEiP0AEU0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75369; AAC75369; b2309.
BAA16155; BAA16155; BAA16155.
GeneIDi945309.
KEGGiecj:JW2306.
eco:b2309.
PATRICi32119989. VBIEscCol129921_2404.

Organism-specific databases

EchoBASEiEB2045.
EcoGeneiEG12124. hisJ.

Phylogenomic databases

eggNOGiENOG4105E89. Bacteria.
COG0834. LUCA.
HOGENOMiHOG000031896.
InParanoidiP0AEU0.
KOiK10014.
OMAiIPYQNQD.
PhylomeDBiP0AEU0.

Enzyme and pathway databases

BioCyciEcoCyc:HISJ-MONOMER.
ECOL316407:JW2306-MONOMER.
MetaCyc:HISJ-MONOMER.
RETL1328306-WGS:GSTH-6770-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AEU0.
PROiP0AEU0.

Family and domain databases

InterProiIPR005768. Lys_Arg_Orn-bd.
IPR018313. SBP_3_CS.
IPR001638. Solute-binding_3/MltF_N.
[Graphical view]
PfamiPF00497. SBP_bac_3. 1 hit.
[Graphical view]
SMARTiSM00062. PBPb. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01096. 3A0103s03R. 1 hit.
PROSITEiPS01039. SBP_BACTERIAL_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHISJ_ECOLI
AccessioniPrimary (citable) accession number: P0AEU0
Secondary accession number(s): P39182, P77763
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.