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Protein

Acid stress chaperone HdeA

Gene

hdeA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for optimal acid stress protection. Exhibits a chaperone-like activity only at pH below 3 by suppressing non-specifically the aggregation of denaturated periplasmic proteins. Important for survival of enteric bacteria in the acidic environment of the host stomach. Also promotes the solubilization at neutral pH of proteins that had aggregated in their presence at acidic pHs. May cooperate with other periplasmic chaperones such as DegP and SurA.4 Publications

Miscellaneous

In vitro, HdeA is more efficient than HdeB at pH 2 and HdeB is more efficient than HdeA at pH 3. In vivo, both are required for optimal protection against acid stress at either pH 3 or pH 2 (PubMed:17085547).1 Publication

GO - Molecular functioni

  • chaperone binding Source: EcoCyc
  • identical protein binding Source: IntAct

GO - Biological processi

  • cellular response to acidic pH Source: EcoCyc

Keywordsi

Molecular functionChaperone

Enzyme and pathway databases

BioCyciEcoCyc:EG11398-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Acid stress chaperone HdeAUniRule annotation
Alternative name(s):
10K-S protein
Gene namesi
Name:hdeAUniRule annotation
Synonyms:yhhC, yhiB
Ordered Locus Names:b3510, JW3478
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11398 hdeA

Subcellular locationi

  • Periplasm UniRule annotation1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21UniRule annotation4 PublicationsAdd BLAST21
ChainiPRO_000002140422 – 110Acid stress chaperone HdeAAdd BLAST89

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi39 ↔ 872 Publications

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP0AES9
PRIDEiP0AES9

2D gel databases

SWISS-2DPAGEiP0AES9

Expressioni

Inductioni

Activated by a low pH-induced dimer-to-monomer transition.

Interactioni

Subunit structurei

Homodimer at neutral pH. Dissociates into monomer at pH 4. Changes from a highly ordered form at pH above 3.1 to a highly disordered form at pH below 2.5 that is essential for its chaperone activity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-15826386,EBI-15826386

GO - Molecular functioni

  • chaperone binding Source: EcoCyc
  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4262518, 8 interactors
DIPiDIP-47945N
STRINGi316385.ECDH10B_3686

Structurei

Secondary structure

1110
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi34 – 36Combined sources3
Helixi39 – 43Combined sources5
Helixi47 – 49Combined sources3
Helixi50 – 62Combined sources13
Helixi66 – 68Combined sources3
Helixi73 – 88Combined sources16
Turni89 – 92Combined sources4
Helixi95 – 103Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BG8X-ray2.20A/B/C22-110[»]
1DJ8X-ray2.00A/B/C/D/E/F22-110[»]
ProteinModelPortaliP0AES9
SMRiP0AES9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AES9

Family & Domainsi

Sequence similaritiesi

Belongs to the HdeA family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG41060V2 Bacteria
ENOG41128BT LUCA
HOGENOMiHOG000125826
KOiK19777
OMAiFNEDITM

Family and domain databases

Gene3Di1.10.890.10, 1 hit
HAMAPiMF_00946 HdeA, 1 hit
InterProiView protein in InterPro
IPR024972 HdeA
IPR038303 HdeA/HdeB_sf
IPR036831 HdeA_sf
IPR010486 HNS-dep_expression_A/B
PfamiView protein in Pfam
PF06411 HdeA, 1 hit
PIRSFiPIRSF009564 HNS-dep_expression_A, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD065392 HNS-dep_expression_A, 1 hit
SUPFAMiSSF47752 SSF47752, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AES9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVLGVILG GLLLLPVVSN AADAQKAADN KKPVNSWTCE DFLAVDESFQ
60 70 80 90 100
PTAVGFAEAL NNKDKPEDAV LDVQGIATVT PAIVQACTQD KQANFKDKVK
110
GEWDKIKKDM
Length:110
Mass (Da):11,858
Last modified:December 20, 2005 - v1
Checksum:i063262C4863FA2E9
GO

Mass spectrometryi

Molecular mass is 9742 Da from positions 22 - 110. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11109 Genomic DNA Translation: BAA01883.1
U00039 Genomic DNA Translation: AAB18486.1
U00096 Genomic DNA Translation: AAC76535.1
AP009048 Genomic DNA Translation: BAE77784.1
PIRiS30268
RefSeqiNP_417967.1, NC_000913.3
WP_000756550.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76535; AAC76535; b3510
BAE77784; BAE77784; BAE77784
GeneIDi948025
KEGGiecj:JW3478
eco:b3510
PATRICifig|511145.12.peg.3617

Similar proteinsi

Entry informationi

Entry nameiHDEA_ECOLI
AccessioniPrimary (citable) accession number: P0AES9
Secondary accession number(s): P26604, Q2M7H2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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