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P0AES5 (GYRA_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA gyrase subunit A
EC=5.99.1.3
Gene names
Name:gyrA
Ordered Locus Names:SF2311, S2444
OrganismShigella flexneri [Complete proteome] [HAMAP]
Taxonomic identifier623 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length875 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP MF_01897

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897

Subunit structure

Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity.

Subcellular location

Cytoplasm Potential HAMAP MF_01897.

Miscellaneous

When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between the gyrA and DNA. HAMAP MF_01897

Sequence similarities

Belongs to the topoisomerase GyrA/ParC subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 875875DNA gyrase subunit A HAMAP MF_01897
PRO_0000145249

Sites

Active site1221O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Secondary structure

1 ........................................................................ 875
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AES5 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 3FD5BD52A5969069

FASTA87596,964
        10         20         30         40         50         60 
MSDLAREITP VNIEEELKSS YLDYAMSVIV GRALPDVRDG LKPVHRRVLY AMNVLGNDWN 

        70         80         90        100        110        120 
KAYKKSARVV GDVIGKYHPH GDSAVYDTIV RMAQPFSLRY MLVDGQGNFG SIDGDSAAAM 

       130        140        150        160        170        180 
RYTEIRLAKI AHELMADLEK ETVDFVDNYD GTEKIPDVMP TKIPNLLVNG SSGIAVGMAT 

       190        200        210        220        230        240 
NIPPHNLTEV INGCLAYIDD EDISIEGLME HIPGPDFPTA AIINGRRGIE EAYRTGRGKV 

       250        260        270        280        290        300 
YIRARAEVEV DAKTGRETII VHEIPYQVNK ARLIEKIAEL VKEKRVEGIS ALRDESDKDG 

       310        320        330        340        350        360 
MRIVIEVKRD AVGEVVLNNL YSQTQLQVSF GINMVALHHG QPKIMNLKDI IAAFVRHRRE 

       370        380        390        400        410        420 
VVTRRTIFEL RKARDRAHIL EALAVALANI DPIIELIRHA PTPAEAKTAL VANPWQLGNV 

       430        440        450        460        470        480 
AAMLERAGDD AARPEWLEPE FGVRDGLYYL TEQQAQAILD LRLQKLTGLE HEKLLDEYKE 

       490        500        510        520        530        540 
LLDQIAELLR ILGSADRLME VIREELELVR EQFGDKRRTE ITANSADINL EDLITQEDVV 

       550        560        570        580        590        600 
VTLSHQGYVK YQPLSEYEAQ RRGGKGKSAA RIKEEDFIDR LLVANTHDHI LCFSSRGRVY 

       610        620        630        640        650        660 
SMKVYQLPEA TRGARGRPIV NLLPLEQDER ITAILPVTEF EEGVKVFMAT ANGTVKKTVL 

       670        680        690        700        710        720 
TEFNRLRTAG KVAIKLVDGD ELIGVDLTSG EDEVMLFSAE GKVVRFKESS VRAMGCNTTG 

       730        740        750        760        770        780 
VRGIRLGEGD KVVSLIVPRG DGAILTATQN GYGKRTAVAE YPTKSRATKG VISIKVTERN 

       790        800        810        820        830        840 
GLVVGAVQVD DCDQIMMITD AGTLVRTRVS EISIVGRNTQ GVILIRTAED ENVVGLQRVA 

       850        860        870 
EPVDEEDLDT IDGSAAEGDD EIAPEVDVDD EPEEE

« Hide

References

[1]"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. expand/collapse author list , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 301 / Serotype 2a.
[2]"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T."
Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.
Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700930 / 2457T / Serotype 2a.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005674 Genomic DNA. Translation: AAN43827.1.
AE014073 Genomic DNA. Translation: AAP17645.1.
RefSeqNP_708120.1. NC_004337.2.
NP_837835.1. NC_004741.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y3PX-ray2.62A/B2-523[»]
ProteinModelPortalP0AES5.
SMRP0AES5. Positions 13-522, 535-841.
ModBaseSearch...

Proteomic databases

PRIDEP0AES5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000088613; EBESCP00000085435; EBESCG00000087657.
EBESCT00000092467; EBESCP00000089103; EBESCG00000091511.
GeneID1027274.
1078730.
GenomeReviewsGene locus SF2311 in contig AE005674_GR.
Gene locus S2444 in contig AE014073_GR.
KEGGsfl:SF2311.
sfx:S2444.
PATRIC18706469. VBIShiFle31049_2710.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000010885.
HOGENOMHBG635183.
OMATGRGRIY.
ProtClustDBPRK05560.

Enzyme and pathway databases

BioCycSFLE198214:AAN43827.1-MONOMER.

Family and domain databases

HAMAPMF_01897. GyrA.
[Tree]
InterProIPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013760. Topo_IIA_cen.
[Graphical view]
Gene3DG3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit.
G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KOK02469.
PfamPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMSSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMsTIGR01063. GyrA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGYRA_SHIFL
AccessionPrimary (citable) accession number: P0AES5
Secondary accession number(s): P09097
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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