##gff-version 3
P0AES1	UniProtKB	Initiator methionine	1	1	.	.	.	Note=Removed;Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Chain	2	619	.	.	.	ID=PRO_0000070444;Note=Bifunctional glutathionylspermidine synthetase/amidase	
P0AES1	UniProtKB	Domain	34	176	.	.	.	Note=Peptidase C51;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00048	
P0AES1	UniProtKB	Region	2	195	.	.	.	Note=Gsp amidase	
P0AES1	UniProtKB	Region	196	205	.	.	.	Note=Linker	
P0AES1	UniProtKB	Region	206	619	.	.	.	Note=Gsp synthetase	
P0AES1	UniProtKB	Active site	59	59	.	.	.	Note=S-(gamma-glutamyl-cysteinyl-glycyl)-cysteine intermediate;Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	58	58	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	64	64	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	78	81	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	149	149	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	316	318	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	316	316	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	318	318	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	330	330	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	330	330	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	332	332	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	335	335	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	391	391	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	392	392	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	446	446	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	498	498	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	533	533	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	539	540	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	568	571	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	582	582	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	603	605	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Binding site	610	610	.	.	.	Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Site	131	131	.	.	.	Note=Increases nucleophilicity of active site Cys%3B for amidase activity;Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Site	316	316	.	.	.	Note=Transition state stabilizer%3B for synthetase activity;Ontology_term=ECO:0000250;evidence=ECO:0000250	
P0AES1	UniProtKB	Modified residue	59	59	.	.	.	Note=Cysteine sulfenic acid (-SOH)%3B transient;Ontology_term=ECO:0000250;evidence=ECO:0000250