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Protein

Sodium/glutamate symport carrier protein

Gene

gltS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the sodium-dependent uptake of extracellular glutamate.

GO - Molecular functioni

  • glutamate:sodium symporter activity Source: EcoCyc
  • symporter activity Source: EcoCyc

GO - Biological processi

  • L-glutamate transmembrane transport Source: GOC
  • transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

BioCyciEcoCyc:GLTS-MONOMER.
ECOL316407:JW3628-MONOMER.
MetaCyc:GLTS-MONOMER.

Protein family/group databases

TCDBi2.A.27.1.1. the glutamate:na(+) symporter (ess) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/glutamate symport carrier protein
Alternative name(s):
Glutamate permease
Gene namesi
Name:gltS
Synonyms:gltC
Ordered Locus Names:b3653, JW3628
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10406. gltS.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 22PeriplasmicSequence analysis
Transmembranei3 – 2119HelicalSequence analysisAdd
BLAST
Topological domaini22 – 3514CytoplasmicSequence analysisAdd
BLAST
Transmembranei36 – 5419HelicalSequence analysisAdd
BLAST
Topological domaini55 – 6814PeriplasmicSequence analysisAdd
BLAST
Transmembranei69 – 8719HelicalSequence analysisAdd
BLAST
Topological domaini88 – 903CytoplasmicSequence analysis
Transmembranei91 – 10919HelicalSequence analysisAdd
BLAST
Topological domaini110 – 1112PeriplasmicSequence analysis
Transmembranei112 – 13221HelicalSequence analysisAdd
BLAST
Topological domaini133 – 15927CytoplasmicSequence analysisAdd
BLAST
Transmembranei160 – 17819HelicalSequence analysisAdd
BLAST
Topological domaini179 – 21335PeriplasmicSequence analysisAdd
BLAST
Transmembranei214 – 24027HelicalSequence analysisAdd
BLAST
Topological domaini241 – 2455CytoplasmicSequence analysis
Transmembranei246 – 26722HelicalSequence analysisAdd
BLAST
Topological domaini268 – 2758PeriplasmicSequence analysis
Transmembranei276 – 29419HelicalSequence analysisAdd
BLAST
Topological domaini295 – 3006CytoplasmicSequence analysis
Transmembranei301 – 32525HelicalSequence analysisAdd
BLAST
Topological domaini326 – 33510PeriplasmicSequence analysis
Transmembranei336 – 35722HelicalSequence analysisAdd
BLAST
Topological domaini358 – 37013CytoplasmicSequence analysisAdd
BLAST
Transmembranei371 – 39828HelicalSequence analysisAdd
BLAST
Topological domaini399 – 4013PeriplasmicSequence analysis

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 401401Sodium/glutamate symport carrier proteinPRO_0000052332Add
BLAST

Proteomic databases

PaxDbiP0AER8.

Interactioni

Protein-protein interaction databases

BioGridi4262574. 14 interactions.
DIPiDIP-48023N.
IntActiP0AER8. 1 interaction.
STRINGi511145.b3653.

Structurei

3D structure databases

ProteinModelPortaliP0AER8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GltS symporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D6U. Bacteria.
COG0786. LUCA.
HOGENOMiHOG000282013.
InParanoidiP0AER8.
KOiK03312.
OMAiPTAMVNM.
OrthoDBiEOG6FRCSX.
PhylomeDBiP0AER8.

Family and domain databases

InterProiIPR004445. GltS.
[Graphical view]
PANTHERiPTHR36178:SF1. PTHR36178:SF1. 1 hit.
PfamiPF03616. Glt_symporter. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00210. gltS. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AER8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFHLDTLATL VAATLTLLLG RKLVHSVSFL KKYTIPEPVA GGLLVALALL
60 70 80 90 100
VLKKSMGWEV NFDMSLRDPL MLAFFATIGL NANIASLRAG GRVVGIFLIV
110 120 130 140 150
VVGLLVMQNA IGIGMASLLG LDPLMGLLAG SITLSGGHGT GAAWSKLFIE
160 170 180 190 200
RYGFTNATEV AMACATFGLV LGGLIGGPVA RYLVKHSTTP NGIPDDQEVP
210 220 230 240 250
TAFEKPDVGR MITSLVLIET IALIAICLTV GKIVAQLLAG TAFELPTFVC
260 270 280 290 300
VLFVGVILSN GLSIMGFYRV FERAVSVLGN VSLSLFLAMA LMGLKLWELA
310 320 330 340 350
SLALPMLAIL VVQTIFMALY AIFVTWRMMG KNYDAAVLAA GHCGFGLGAT
360 370 380 390 400
PTAIANMQAI TERFGPSHMA FLVVPMVGAF FIDIVNALVI KLYLMLPIFA

G
Length:401
Mass (Da):42,425
Last modified:December 20, 2005 - v1
Checksum:i9569AE0AF75634EC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti378 – 3781G → S in BAA00517 (PubMed:2254324).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00626 Genomic DNA. Translation: BAA00517.1.
X17499 Genomic DNA. Translation: CAA35540.1.
L10328 Genomic DNA. Translation: AAA62006.1.
U00096 Genomic DNA. Translation: AAC76677.1.
AP009048 Genomic DNA. Translation: BAE77640.1.
M64367 Genomic DNA. Translation: AAA24514.1.
PIRiG65166. YOECNQ.
RefSeqiNP_418110.1. NC_000913.3.
WP_000468833.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76677; AAC76677; b3653.
BAE77640; BAE77640; BAE77640.
GeneIDi948166.
KEGGiecj:JW3628.
eco:b3653.
PATRICi32122793. VBIEscCol129921_3773.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00626 Genomic DNA. Translation: BAA00517.1.
X17499 Genomic DNA. Translation: CAA35540.1.
L10328 Genomic DNA. Translation: AAA62006.1.
U00096 Genomic DNA. Translation: AAC76677.1.
AP009048 Genomic DNA. Translation: BAE77640.1.
M64367 Genomic DNA. Translation: AAA24514.1.
PIRiG65166. YOECNQ.
RefSeqiNP_418110.1. NC_000913.3.
WP_000468833.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AER8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262574. 14 interactions.
DIPiDIP-48023N.
IntActiP0AER8. 1 interaction.
STRINGi511145.b3653.

Protein family/group databases

TCDBi2.A.27.1.1. the glutamate:na(+) symporter (ess) family.

Proteomic databases

PaxDbiP0AER8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76677; AAC76677; b3653.
BAE77640; BAE77640; BAE77640.
GeneIDi948166.
KEGGiecj:JW3628.
eco:b3653.
PATRICi32122793. VBIEscCol129921_3773.

Organism-specific databases

EchoBASEiEB0401.
EcoGeneiEG10406. gltS.

Phylogenomic databases

eggNOGiENOG4105D6U. Bacteria.
COG0786. LUCA.
HOGENOMiHOG000282013.
InParanoidiP0AER8.
KOiK03312.
OMAiPTAMVNM.
OrthoDBiEOG6FRCSX.
PhylomeDBiP0AER8.

Enzyme and pathway databases

BioCyciEcoCyc:GLTS-MONOMER.
ECOL316407:JW3628-MONOMER.
MetaCyc:GLTS-MONOMER.

Miscellaneous databases

PROiP0AER8.

Family and domain databases

InterProiIPR004445. GltS.
[Graphical view]
PANTHERiPTHR36178:SF1. PTHR36178:SF1. 1 hit.
PfamiPF03616. Glt_symporter. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00210. gltS. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of gltS, the Na+/glutamate symport carrier gene of Escherichia coli B."
    Deguchi Y., Yamato I., Anraku Y.
    J. Biol. Chem. 265:21704-21708(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: B.
  2. "Characterization of the Escherichia coli K12 gltS glutamate permease gene."
    Kalman M., Gentry D., Cashel M.
    Mol. Gen. Genet. 225:379-386(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
    Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
    Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "The nucleotide sequence of recG, the distal spo operon gene in Escherichia coli K-12."
    Kalman M., Murphy H., Cashel M.
    Gene 110:95-99(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 379-401.
    Strain: K12.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiGLTS_ECOLI
AccessioniPrimary (citable) accession number: P0AER8
Secondary accession number(s): P19933, Q2M7W6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 11, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.