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Protein

Sodium/glutamate symporter

Gene

gltS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the sodium-dependent, binding-protein-independent transport of glutamate.UniRule annotation3 Publications

Enzyme regulationi

Inhibited by the uncoupler carbonylcyanide m-chlorophenylhydrazone (CCCP) and the ionophore monensin.1 Publication

Kineticsi

    1. Vmax=5.2 nmol/min/mg enzyme1 Publication

    GO - Molecular functioni

    • glutamate:sodium symporter activity Source: EcoCyc
    • symporter activity Source: EcoCyc
    Complete GO annotation...

    Keywords - Biological processi

    Amino-acid transport, Ion transport, Sodium transport, Symport, Transport

    Keywords - Ligandi

    Sodium

    Enzyme and pathway databases

    BioCyciEcoCyc:GLTS-MONOMER.
    ECOL316407:JW3628-MONOMER.
    MetaCyc:GLTS-MONOMER.

    Protein family/group databases

    TCDBi2.A.27.1.1. the glutamate:na(+) symporter (ess) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/glutamate symporterUniRule annotationCurated
    Alternative name(s):
    Glutamate permease1 Publication
    Gene namesi
    Name:gltSUniRule annotation
    Synonyms:gltC
    Ordered Locus Names:b3653, JW3628
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10406. gltS.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 6Periplasmic2 Publications6
    Transmembranei7 – 24HelicalSequence analysisAdd BLAST18
    Topological domaini25 – 32Cytoplasmic2 Publications8
    Transmembranei33 – 52HelicalSequence analysisAdd BLAST20
    Topological domaini53 – 69Periplasmic2 PublicationsAdd BLAST17
    Transmembranei70 – 87HelicalSequence analysisAdd BLAST18
    Topological domaini88 – 93Cytoplasmic2 Publications6
    Transmembranei94 – 116HelicalSequence analysisAdd BLAST23
    Topological domaini117 – 156Periplasmic1 PublicationAdd BLAST40
    Transmembranei157 – 179HelicalSequence analysisAdd BLAST23
    Topological domaini180 – 212Cytoplasmic1 PublicationAdd BLAST33
    Transmembranei213 – 235HelicalSequence analysisAdd BLAST23
    Topological domaini236 – 244Periplasmic2 Publications9
    Transmembranei245 – 267HelicalSequence analysisAdd BLAST23
    Topological domaini268 – 276Cytoplasmic2 Publications9
    Transmembranei277 – 292HelicalSequence analysisAdd BLAST16
    Topological domaini293 – 301Periplasmic2 Publications9
    Transmembranei302 – 324HelicalSequence analysisAdd BLAST23
    Topological domaini325 – 367Cytoplasmic2 PublicationsAdd BLAST43
    Transmembranei368 – 390HelicalSequence analysisAdd BLAST23
    Topological domaini391 – 401Periplasmic2 Publications1 PublicationAdd BLAST11

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: EcoCyc
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000523321 – 401Sodium/glutamate symporterAdd BLAST401

    Proteomic databases

    PaxDbiP0AER8.
    PRIDEiP0AER8.

    Interactioni

    Protein-protein interaction databases

    BioGridi4262574. 14 interactors.
    DIPiDIP-48023N.
    IntActiP0AER8. 1 interactor.
    STRINGi511145.b3653.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AER8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamate:Na(+) symporter (ESS) (TC 2.A.27) family. [View classification]UniRule annotationCurated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105D6U. Bacteria.
    COG0786. LUCA.
    HOGENOMiHOG000282013.
    InParanoidiP0AER8.
    KOiK03312.
    OMAiPTAMVNM.
    PhylomeDBiP0AER8.

    Family and domain databases

    HAMAPiMF_02062. GltS. 1 hit.
    InterProiIPR004445. GltS.
    [Graphical view]
    PANTHERiPTHR36178:SF1. PTHR36178:SF1. 1 hit.
    PfamiPF03616. Glt_symporter. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00210. gltS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0AER8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MFHLDTLATL VAATLTLLLG RKLVHSVSFL KKYTIPEPVA GGLLVALALL
    60 70 80 90 100
    VLKKSMGWEV NFDMSLRDPL MLAFFATIGL NANIASLRAG GRVVGIFLIV
    110 120 130 140 150
    VVGLLVMQNA IGIGMASLLG LDPLMGLLAG SITLSGGHGT GAAWSKLFIE
    160 170 180 190 200
    RYGFTNATEV AMACATFGLV LGGLIGGPVA RYLVKHSTTP NGIPDDQEVP
    210 220 230 240 250
    TAFEKPDVGR MITSLVLIET IALIAICLTV GKIVAQLLAG TAFELPTFVC
    260 270 280 290 300
    VLFVGVILSN GLSIMGFYRV FERAVSVLGN VSLSLFLAMA LMGLKLWELA
    310 320 330 340 350
    SLALPMLAIL VVQTIFMALY AIFVTWRMMG KNYDAAVLAA GHCGFGLGAT
    360 370 380 390 400
    PTAIANMQAI TERFGPSHMA FLVVPMVGAF FIDIVNALVI KLYLMLPIFA

    G
    Length:401
    Mass (Da):42,425
    Last modified:December 20, 2005 - v1
    Checksum:i9569AE0AF75634EC
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti378G → S in BAA00517 (PubMed:2254324).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D00626 Genomic DNA. Translation: BAA00517.1.
    X17499 Genomic DNA. Translation: CAA35540.1.
    L10328 Genomic DNA. Translation: AAA62006.1.
    U00096 Genomic DNA. Translation: AAC76677.1.
    AP009048 Genomic DNA. Translation: BAE77640.1.
    M64367 Genomic DNA. Translation: AAA24514.1.
    PIRiG65166. YOECNQ.
    RefSeqiNP_418110.1. NC_000913.3.
    WP_000468833.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76677; AAC76677; b3653.
    BAE77640; BAE77640; BAE77640.
    GeneIDi948166.
    KEGGiecj:JW3628.
    eco:b3653.
    PATRICi32122793. VBIEscCol129921_3773.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D00626 Genomic DNA. Translation: BAA00517.1.
    X17499 Genomic DNA. Translation: CAA35540.1.
    L10328 Genomic DNA. Translation: AAA62006.1.
    U00096 Genomic DNA. Translation: AAC76677.1.
    AP009048 Genomic DNA. Translation: BAE77640.1.
    M64367 Genomic DNA. Translation: AAA24514.1.
    PIRiG65166. YOECNQ.
    RefSeqiNP_418110.1. NC_000913.3.
    WP_000468833.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP0AER8.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262574. 14 interactors.
    DIPiDIP-48023N.
    IntActiP0AER8. 1 interactor.
    STRINGi511145.b3653.

    Protein family/group databases

    TCDBi2.A.27.1.1. the glutamate:na(+) symporter (ess) family.

    Proteomic databases

    PaxDbiP0AER8.
    PRIDEiP0AER8.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC76677; AAC76677; b3653.
    BAE77640; BAE77640; BAE77640.
    GeneIDi948166.
    KEGGiecj:JW3628.
    eco:b3653.
    PATRICi32122793. VBIEscCol129921_3773.

    Organism-specific databases

    EchoBASEiEB0401.
    EcoGeneiEG10406. gltS.

    Phylogenomic databases

    eggNOGiENOG4105D6U. Bacteria.
    COG0786. LUCA.
    HOGENOMiHOG000282013.
    InParanoidiP0AER8.
    KOiK03312.
    OMAiPTAMVNM.
    PhylomeDBiP0AER8.

    Enzyme and pathway databases

    BioCyciEcoCyc:GLTS-MONOMER.
    ECOL316407:JW3628-MONOMER.
    MetaCyc:GLTS-MONOMER.

    Miscellaneous databases

    PROiP0AER8.

    Family and domain databases

    HAMAPiMF_02062. GltS. 1 hit.
    InterProiIPR004445. GltS.
    [Graphical view]
    PANTHERiPTHR36178:SF1. PTHR36178:SF1. 1 hit.
    PfamiPF03616. Glt_symporter. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00210. gltS. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGLTS_ECOLI
    AccessioniPrimary (citable) accession number: P0AER8
    Secondary accession number(s): P19933, Q2M7W6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: November 2, 2016
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.