P0AER3 (GLTJ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate/aspartate transport system permease protein GltJ

Gene names

Name:	gltJ
Ordered Locus Names:	b0654, JW0649

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.6.
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. HisMQ subfamily. Contains 1 ABC transmembrane type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Glutamate/aspartate transport system permease protein GltJ		PRO_0000060035
Regions
	Transmembrane	33 – 53	21	Helical; Potential
	Transmembrane	74 – 94	21	Helical; Potential
	Transmembrane	104 – 124	21	Helical; Potential
	Transmembrane	179 – 196	18	Helical; Potential
	Transmembrane	212 – 232	21	Helical; Potential
	Domain	29 – 230	202	ABC transmembrane type-1

Sequences

	Sequence		Length	Mass (Da)
	P0AER3 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: E9E5CAF26BD252B8	FASTA	246	27,503
	10 20 30 40 50 60 MSIDWNWGIF LQQAPFGNTT YLGWIWSGFQ VTIALSICAW IIAFLVGSFF GILRTVPNRF 70 80 90 100 110 120 LSGLGTLYVE LFRNVPLIVQ FFTWYLVIPE LLPEKIGMWF KAELDPNIQF FLSSMLCLGL 130 140 150 160 170 180 FTAARVCEQV RAAIQSLPRG QKNAALAMGL TLPQAYRYVL LPNAYRVIVP PMTSEMMNLV 190 200 210 220 230 240 KNSAIASTIG LVDMAAQAGK LLDYSAHAWE SFTAITLAYV LINAFIMLVM TLVERKVRLP GNMGGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and characterisation of three genes of a glutamate-aspartate binding protein-dependent transport system of Escherichia coli K12." Lum D., Wallace B.J. Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / BK9MDG.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10981 Genomic DNA. Translation: AAA60980.1. U82598 Genomic DNA. Translation: AAB40855.1. U00096 Genomic DNA. Translation: AAC73755.1. AP009048 Genomic DNA. Translation: BAA35306.1.
PIR	D64800.
RefSeq	NP_415187.1. NC_000913.2. YP_488945.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AER3.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-35965N.
IntAct	P0AER3. 6 interactions.
MINT	MINT-1264109.
STRING	511145.b0654.
Protein family/group databases
TCDB	3.A.1.3.4. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PRIDE	P0AER3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73755; AAC73755; b0654. BAA35306; BAA35306; BAA35306.
GeneID	12930920. 945443.
KEGG	ecj:Y75_p0644. eco:b0654.
PATRIC	32116491. VBIEscCol129921_0685.
Organism-specific databases
EchoBASE	EB2527.
EcoGene	EG12661. gltJ.
Phylogenomic databases
eggNOG	COG0765.
HOGENOM	HOG000267552.
KO	K10003.
OMA	PGNMGSK.
ProtClustDB	CLSK879719.
Enzyme and pathway databases
BioCyc	EcoCyc:GLTJ-MONOMER. ECOL316407:JW0649-MONOMER.
Gene expression databases
Genevestigator	P0AER3.
Family and domain databases
InterPro	IPR010065. AA_ABC_transptr_permease_3TM. IPR000515. BPD_transp. [Graphical view]
Pfam	PF00528. BPD_transp_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01726. HEQRo_perm_3TM. 1 hit.
PROSITE	PS50928. ABC_TM1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLTJ_ECOLI

Accession

Primary (citable) accession number: P0AER3
Secondary accession number(s): P41074

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program