P0AEP9 (GLCD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycolate oxidase subunit GlcD

Gene names

Name:	glcD
Synonyms:	gox, yghM
Ordered Locus Names:	b2979, JW2946

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor	FAD Potential.
Sequence similarities	Belongs to the FAD-binding oxidoreductase/transferase type 4 family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolate catabolic process Inferred from mutant phenotype Ref.1. Source: EcoCyc response to DNA damage stimulus Inferred from expression pattern PubMed 11967071. Source: EcoliWiki
Cellular_component	glycolate oxidase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro glycolate dehydrogenase activity Inferred from mutant phenotype Ref.1. Source: EcoCyc glycolate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Glycolate oxidase subunit GlcD		PRO_0000128181
Regions
	Domain	52 – 230	179	FAD-binding PCMH-type

Sequences

	Sequence		Length	Mass (Da)
	P0AEP9 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 2D850E109DD1BA55	FASTA	499	53,812
	10 20 30 40 50 60 MSILYEERLD GALPDVDRTS VLMALREHVP GLEILHTDEE IIPYECDGLS AYRTRPLLVV 70 80 90 100 110 120 LPKQMEQVTA ILAVCHRLRV PVVTRGAGTG LSGGALPLEK GVLLVMARFK EILDINPVGR 130 140 150 160 170 180 RARVQPGVRN LAISQAVAPH NLYYAPDPSS QIACSIGGNV AENAGGVHCL KYGLTVHNLL 190 200 210 220 230 240 KIEVQTLDGE ALTLGSDALD SPGFDLLALF TGSEGMLGVT TEVTVKLLPK PPVARVLLAS 250 260 270 280 290 300 FDSVEKAGLA VGDIIANGII PGGLEMMDNL SIRAAEDFIH AGYPVDAEAI LLCELDGVES 310 320 330 340 350 360 DVQEDCERVN DILLKAGATD VRLAQDEAER VRFWAGRKNA FPAVGRISPD YYCMDGTIPR 370 380 390 400 410 420 RALPGVLEGI ARLSQQYDLR VANVFHAGDG NMHPLILFDA NEPGEFARAE ELGGKILELC 430 440 450 460 470 480 VEVGGSISGE HGIGREKINQ MCAQFNSDEI TTFHAVKAAF DPDGLLNPGK NIPTLHRCAE 490 FGAMHVHHGH LPFPELERF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"glc locus of Escherichia coli: characterization of genes encoding the subunits of glycolate oxidase and the glc regulator protein." Pellicer M.T., Badia J., Aguilar J.T., Baldoma L. J. Bacteriol. 178:2051-2059(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43490 Genomic DNA. Translation: AAB02530.1. U28377 Genomic DNA. Translation: AAA69146.1. U00096 Genomic DNA. Translation: AAC76015.1. AP009048 Genomic DNA. Translation: BAE77040.1.
PIR	A65084.
RefSeq	NP_417453.1. NC_000913.2. YP_491176.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AEP9.
SMR	P0AEP9. Positions 40-470.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b2979.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76015; AAC76015; b2979. BAE77040; BAE77040; BAE77040.
GeneID	12933374. 947353.
KEGG	ecj:Y75_p2908. eco:b2979.
PATRIC	32121374. VBIEscCol129921_3074.
Organism-specific databases
EchoBASE	EB2820.
EcoGene	EG12997. glcD.
Phylogenomic databases
eggNOG	COG0277.
HOGENOM	HOG000230998.
KO	K00104.
OMA	CVKVGGS.
ProtClustDB	PRK11230.
Enzyme and pathway databases
BioCyc	EcoCyc:G7545-MONOMER. ECOL316407:JW2946-MONOMER. MetaCyc:G7545-MONOMER.
Gene expression databases
Genevestigator	P0AEP9.
Family and domain databases
Gene3D	1.10.45.10. 1 hit. 3.30.43.10. 1 hit. 3.30.465.10. 1 hit.
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR016164. FAD-linked_Oxase-like_C. IPR004113. FAD-linked_oxidase_C. IPR004490. GlcD. IPR006094. Oxid_FAD_bind_N. IPR016171. Vanillyl_alc_oxidase_C-sub2. [Graphical view]
Pfam	PF02913. FAD-oxidase_C. 1 hit. PF01565. FAD_binding_4. 1 hit. [Graphical view]
SUPFAM	SSF55103. FAD-binding_2. 1 hit. SSF56176. FAD-binding_2. 1 hit.
TIGRFAMs	TIGR00387. glcD. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLCD_ECOLI

Accession

Primary (citable) accession number: P0AEP9
Secondary accession number(s): P52075, Q2M9L6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program