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P0AEP7 (GCL_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glyoxylate carboligase
EC=4.1.1.47
Alternative name(s):
Tartronate-semialdehyde synthase
Gene names
Name:gcl
Ordered Locus Names:b0507, JW0495
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length593 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde).

Catalytic activity

2 glyoxylate = tartronate semialdehyde + CO2.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Organic acid metabolism; glycolate degradation; 3-phospho-D-glycerate from glycolate: step 2/4.

Subunit structure

Homotetramer.

Induction

By glyoxylate.

Sequence similarities

Belongs to the TPP enzyme family.

Sequence caution

The sequence AAB40260.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 593592Glyoxylate carboligase
PRO_0000090832

Secondary structure

1 ................................................................................................................... 593
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AEP7 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: EF35ECC43B7C62E7

FASTA59364,732
        10         20         30         40         50         60 
MAKMRAVDAA MYVLEKEGIT TAFGVPGAAI NPFYSAMRKH GGIRHILARH VEGASHMAEG 

        70         80         90        100        110        120 
YTRATAGNIG VCLGTSGPAG TDMITALYSA SADSIPILCI TGQAPRARLH KEDFQAVDIE 

       130        140        150        160        170        180 
AIAKPVSKMA VTVREAALVP RVLQQAFHLM RSGRPGPVLV DLPFDVQVAE IEFDPDMYEP 

       190        200        210        220        230        240 
LPVYKPAASR MQIEKAVEML IQAERPVIVA GGGVINADAA ALLQQFAELT SVPVIPTLMG 

       250        260        270        280        290        300 
WGCIPDDHEL MAGMVGLQTA HRYGNATLLA SDMVFGIGNR FANRHTGSVE KYTEGRKIVH 

       310        320        330        340        350        360 
IDIEPTQIGR VLCPDLGIVS DAKAALTLLV EVAQEMQKAG RLPCRKEWVA DCQQRKRTLL 

       370        380        390        400        410        420 
RKTHFDNVPV KPQRVYEEMN KAFGRDVCYV TTIGLSQIAA AQMLHVFKDR HWINCGQAGP 

       430        440        450        460        470        480 
LGWTIPAALG VCAADPKRNV VAISGDFDFQ FLIEELAVGA QFNIPYIHVL VNNAYLGLIR 

       490        500        510        520        530        540 
QSQRAFDMDY CVQLAFENIN SSEVNGYGVD HVKVAEGLGC KAIRVFKPED IAPAFEQAKA 

       550        560        570        580        590 
LMAQYRVPVV VEVILERVTN ISMGSELDNV MEFEDIADNA ADAPTETCFM HYE

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning, DNA sequencing, and biochemical analyses of Escherichia coli glyoxylate carboligase. An enzyme of the acetohydroxy acid synthase-pyruvate oxidase family."
Chang Y.-Y., Wang A.-Y., Cronan J.E. Jr.
J. Biol. Chem. 268:3911-3919(1993) [PubMed: 8440684] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-31.
Strain: K12.
[2]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L03845 Genomic DNA. Translation: AAA23864.1.
U82664 Genomic DNA. Translation: AAB40260.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73609.1.
AP009048 Genomic DNA. Translation: BAE76285.1.
PIRJT0742.
RefSeqNP_415040.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2PANX-ray2.70A/B/C/D/E/F1-593[»]
ProteinModelPortalP0AEP7.
SMRP0AEP7. Positions 2-593.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-48112N.
IntActP0AEP7. 6 interactions.
MINTMINT-1302810.

Proteomic databases

PRIDEP0AEP7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000000808; EBESCP00000000808; EBESCG00000000674.
EBESCT00000016397; EBESCP00000015688; EBESCG00000015457.
GeneID945394.
GenomeReviewsGene locus JW0495 in contig AP009048_GR.
Gene locus b0507 in contig U00096_GR.
KEGGecj:JW0495.
eco:b0507.
PATRIC32116173. VBIEscCol129921_0528.

Organism-specific databases

EchoBASEEB1542.
EcoGeneEG11583. gcl.

Phylogenomic databases

eggNOGCOG3960.
GeneTreeEBGT00050000008285.
HOGENOMHBG323037.
OMAEGYTRAK.
PhylomeDBP0AEP7.
ProtClustDBPRK11269.

Enzyme and pathway databases

BioCycEcoCyc:GLYOCARBOLIG-MONOMER.
MetaCyc:GLYOCARBOLIG-MONOMER.

Gene expression databases

GenevestigatorP0AEP7.

Family and domain databases

InterProIPR006397. Glyox_carbo_lig.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
KOK01608.
PANTHERPTHR18968:SF14. PTHR18968:SF14. 1 hit.
PfamPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01504. Glyox_carbo_lig. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGCL_ECOLI
AccessionPrimary (citable) accession number: P0AEP7
Secondary accession number(s): P30146, P77126, Q2MBS1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: January 25, 2012
This is version 52 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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