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Protein

UTP--glucose-1-phosphate uridylyltransferase

Gene

galU

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in stationary phase survival.By similarity

Catalytic activityi

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Cofactori

Mg2+By similarity

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis, Virulence

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

UniPathwayiUPA00030.

Names & Taxonomyi

Protein namesi
Recommended name:
UTP--glucose-1-phosphate uridylyltransferase (EC:2.7.7.9)
Alternative name(s):
Alpha-D-glucosyl-1-phosphate uridylyltransferase
UDP-glucose pyrophosphorylase
Short name:
UDPGP
Uridine diphosphoglucose pyrophosphorylase
Gene namesi
Name:galU
Ordered Locus Names:SF1236, S1322
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

A transposon mutant in galU (only the first 220 residues could be expressed) allows HeLa cell invasion but no cell spreading. Shows decreased secretion of IscA, a protein required for cell spreading. The lipopolysaccharide produced is of a size consistent with it containing only lipid A and a single core component.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002013632 – 302UTP--glucose-1-phosphate uridylyltransferaseAdd BLAST301

Proteomic databases

PaxDbiP0AEP6.
PRIDEiP0AEP6.

Interactioni

Subunit structurei

Homotetramer or homopentamer.By similarity

Protein-protein interaction databases

STRINGi198214.SF1236.

Structurei

3D structure databases

ProteinModelPortaliP0AEP6.
SMRiP0AEP6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDPGP type 2 family.Curated

Phylogenomic databases

eggNOGiENOG4105C3Y. Bacteria.
COG1210. LUCA.
HOGENOMiHOG000283477.
KOiK00963.
OMAiIGYMQAF.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005771. GalU_uridylyltTrfase_bac/arc.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01099. galU. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEP6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAINTKVKK AVIPVAGLGT RMLPATKAIP KEMLPLVDKP LIQYVVNECI
60 70 80 90 100
AAGITEIVLV THSSKNSIEN HFDTSFELEA MLEKRVKRQL LDEVQSICPP
110 120 130 140 150
HVTIMQVRQG LAKGLGHAVL CAHPVVGDEP VAVILPDVIL DEYESDLSQD
160 170 180 190 200
NLAEMIRRFD ETGHSQIMVE PVADVTAYGV VDCKGVELAP GESVPMVGVV
210 220 230 240 250
EKPKADVAPS NLAIVGRYVL SADIWPLLAK TPPGAGDEIQ LTDAIDMLIE
260 270 280 290 300
KETVEAYHMK GKSHDCGNKL GYMQAFVEYG IRHNTLGTEF KAWLEEEMGI

KK
Length:302
Mass (Da):32,942
Last modified:January 23, 2007 - v2
Checksum:iADEAEB3F6A1194CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32811 Genomic DNA. Translation: AAD15244.1.
AE005674 Genomic DNA. Translation: AAN42849.1.
AE014073 Genomic DNA. Translation: AAP16734.1.
RefSeqiNP_707142.1. NC_004337.2.
WP_000718995.1. NZ_LVJC01000023.1.

Genome annotation databases

EnsemblBacteriaiAAN42849; AAN42849; SF1236.
AAP16734; AAP16734; S1322.
GeneIDi1024213.
KEGGisfl:SF1236.
sft:NCTC1_01306.
sfx:S1322.
PATRICi18703882. VBIShiFle31049_1445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32811 Genomic DNA. Translation: AAD15244.1.
AE005674 Genomic DNA. Translation: AAN42849.1.
AE014073 Genomic DNA. Translation: AAP16734.1.
RefSeqiNP_707142.1. NC_004337.2.
WP_000718995.1. NZ_LVJC01000023.1.

3D structure databases

ProteinModelPortaliP0AEP6.
SMRiP0AEP6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.SF1236.

Proteomic databases

PaxDbiP0AEP6.
PRIDEiP0AEP6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN42849; AAN42849; SF1236.
AAP16734; AAP16734; S1322.
GeneIDi1024213.
KEGGisfl:SF1236.
sft:NCTC1_01306.
sfx:S1322.
PATRICi18703882. VBIShiFle31049_1445.

Phylogenomic databases

eggNOGiENOG4105C3Y. Bacteria.
COG1210. LUCA.
HOGENOMiHOG000283477.
KOiK00963.
OMAiIGYMQAF.

Enzyme and pathway databases

UniPathwayiUPA00030.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005771. GalU_uridylyltTrfase_bac/arc.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01099. galU. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGALU_SHIFL
AccessioniPrimary (citable) accession number: P0AEP6
Secondary accession number(s): P25520
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.