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P0AEP6 (GALU_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UTP--glucose-1-phosphate uridylyltransferase

EC=2.7.7.9
Alternative name(s):
Alpha-D-glucosyl-1-phosphate uridylyltransferase
UDP-glucose pyrophosphorylase
Short name=UDPGP
Uridine diphosphoglucose pyrophosphorylase
Gene names
Name:galU
Ordered Locus Names:SF1236, S1322
OrganismShigella flexneri [Complete proteome] [HAMAP]
Taxonomic identifier623 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in stationary phase survival By similarity. Ref.1

Catalytic activity

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Cofactor

Magnesium By similarity.

Pathway

Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.

Subunit structure

Homotetramer or homopentamer By similarity.

Disruption phenotype

A transposon mutant in galU (only the first 220 residues could be expressed) allows HeLa cell invasion but no cell spreading. Shows decreased secretion of IscA, a protein required for cell spreading. The lipopolysaccharide produced is of a size consistent with it containing only lipid A and a single core component. Ref.1

Sequence similarities

Belongs to the UDPGP type 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 302301UTP--glucose-1-phosphate uridylyltransferase
PRO_0000201363

Sequences

Sequence LengthMass (Da)Tools
P0AEP6 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: ADEAEB3F6A1194CB

FASTA30232,942
        10         20         30         40         50         60 
MAAINTKVKK AVIPVAGLGT RMLPATKAIP KEMLPLVDKP LIQYVVNECI AAGITEIVLV 

        70         80         90        100        110        120 
THSSKNSIEN HFDTSFELEA MLEKRVKRQL LDEVQSICPP HVTIMQVRQG LAKGLGHAVL 

       130        140        150        160        170        180 
CAHPVVGDEP VAVILPDVIL DEYESDLSQD NLAEMIRRFD ETGHSQIMVE PVADVTAYGV 

       190        200        210        220        230        240 
VDCKGVELAP GESVPMVGVV EKPKADVAPS NLAIVGRYVL SADIWPLLAK TPPGAGDEIQ 

       250        260        270        280        290        300 
LTDAIDMLIE KETVEAYHMK GKSHDCGNKL GYMQAFVEYG IRHNTLGTEF KAWLEEEMGI 


KK 

« Hide

References

« Hide 'large scale' references
[1]"Avirulence of rough mutants of Shigella flexneri: requirement of O antigen for correct unipolar localization of IcsA in the bacterial outer membrane."
Sandlin R.C., Lampel K.A., Keasler S.P., Goldberg M.B., Stolzer A.L., Maurelli A.T.
Infect. Immun. 63:229-237(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION OF ROLE IN LPS SYNTHESIS AND VIRULENCE, DISRUPTION PHENOTYPE.
Strain: ATCC 700930 / 2457T / Serotype 2a.
[2]"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. expand/collapse author list , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 301 / Serotype 2a.
[3]"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T."
Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.
Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L32811 Genomic DNA. Translation: AAD15244.1.
AE005674 Genomic DNA. Translation: AAN42849.1.
AE014073 Genomic DNA. Translation: AAP16734.1.
RefSeqNP_707142.1. NC_004337.2.
NP_836927.1. NC_004741.1.

3D structure databases

ProteinModelPortalP0AEP6.
SMRP0AEP6. Positions 4-297.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198214.SF1236.

Proteomic databases

PaxDbP0AEP6.
PRIDEP0AEP6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN42849; AAN42849; SF1236.
AAP16734; AAP16734; S1322.
GeneID1024213.
1077674.
KEGGsfl:SF1236.
sfx:S1322.
PATRIC18703882. VBIShiFle31049_1445.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1210.
HOGENOMHOG000283477.
KOK00963.
OMASICPKHV.
OrthoDBEOG6Z9B3V.

Enzyme and pathway databases

UniPathwayUPA00030.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR005771. GalU_uridylyltTrfase_bac/arc.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMSSF53448. SSF53448. 1 hit.
TIGRFAMsTIGR01099. galU. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGALU_SHIFL
AccessionPrimary (citable) accession number: P0AEP6
Secondary accession number(s): P25520
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways