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P0AEP6

- GALU_SHIFL

UniProt

P0AEP6 - GALU_SHIFL

Protein

UTP--glucose-1-phosphate uridylyltransferase

Gene

galU

Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    May play a role in stationary phase survival.By similarity

    Catalytic activityi

    UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

    Cofactori

    Magnesium.By similarity

    Pathwayi

    GO - Molecular functioni

    1. UTP:glucose-1-phosphate uridylyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
    2. pathogenesis Source: UniProtKB-KW
    3. UDP-glucose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Lipopolysaccharide biosynthesis, Virulence

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    UniPathwayiUPA00030.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UTP--glucose-1-phosphate uridylyltransferase (EC:2.7.7.9)
    Alternative name(s):
    Alpha-D-glucosyl-1-phosphate uridylyltransferase
    UDP-glucose pyrophosphorylase
    Short name:
    UDPGP
    Uridine diphosphoglucose pyrophosphorylase
    Gene namesi
    Name:galU
    Ordered Locus Names:SF1236, S1322
    OrganismiShigella flexneri
    Taxonomic identifieri623 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
    ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

    Pathology & Biotechi

    Disruption phenotypei

    A transposon mutant in galU (only the first 220 residues could be expressed) allows HeLa cell invasion but no cell spreading. Shows decreased secretion of IscA, a protein required for cell spreading. The lipopolysaccharide produced is of a size consistent with it containing only lipid A and a single core component.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 302301UTP--glucose-1-phosphate uridylyltransferasePRO_0000201363Add
    BLAST

    Proteomic databases

    PaxDbiP0AEP6.
    PRIDEiP0AEP6.

    Interactioni

    Subunit structurei

    Homotetramer or homopentamer.By similarity

    Protein-protein interaction databases

    STRINGi198214.SF1236.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AEP6.
    SMRiP0AEP6. Positions 4-297.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UDPGP type 2 family.Curated

    Phylogenomic databases

    eggNOGiCOG1210.
    HOGENOMiHOG000283477.
    KOiK00963.
    OMAiSICPKHV.
    OrthoDBiEOG6Z9B3V.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR005771. GalU_uridylyltTrfase_bac/arc.
    IPR005835. NTP_transferase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF00483. NTP_transferase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.
    TIGRFAMsiTIGR01099. galU. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AEP6-1 [UniParc]FASTAAdd to Basket

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    MAAINTKVKK AVIPVAGLGT RMLPATKAIP KEMLPLVDKP LIQYVVNECI    50
    AAGITEIVLV THSSKNSIEN HFDTSFELEA MLEKRVKRQL LDEVQSICPP 100
    HVTIMQVRQG LAKGLGHAVL CAHPVVGDEP VAVILPDVIL DEYESDLSQD 150
    NLAEMIRRFD ETGHSQIMVE PVADVTAYGV VDCKGVELAP GESVPMVGVV 200
    EKPKADVAPS NLAIVGRYVL SADIWPLLAK TPPGAGDEIQ LTDAIDMLIE 250
    KETVEAYHMK GKSHDCGNKL GYMQAFVEYG IRHNTLGTEF KAWLEEEMGI 300
    KK 302
    Length:302
    Mass (Da):32,942
    Last modified:January 23, 2007 - v2
    Checksum:iADEAEB3F6A1194CB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L32811 Genomic DNA. Translation: AAD15244.1.
    AE005674 Genomic DNA. Translation: AAN42849.1.
    AE014073 Genomic DNA. Translation: AAP16734.1.
    RefSeqiNP_707142.1. NC_004337.2.
    NP_836927.1. NC_004741.1.

    Genome annotation databases

    EnsemblBacteriaiAAN42849; AAN42849; SF1236.
    AAP16734; AAP16734; S1322.
    GeneIDi1024213.
    1077674.
    KEGGisfl:SF1236.
    sfx:S1322.
    PATRICi18703882. VBIShiFle31049_1445.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L32811 Genomic DNA. Translation: AAD15244.1 .
    AE005674 Genomic DNA. Translation: AAN42849.1 .
    AE014073 Genomic DNA. Translation: AAP16734.1 .
    RefSeqi NP_707142.1. NC_004337.2.
    NP_836927.1. NC_004741.1.

    3D structure databases

    ProteinModelPortali P0AEP6.
    SMRi P0AEP6. Positions 4-297.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198214.SF1236.

    Proteomic databases

    PaxDbi P0AEP6.
    PRIDEi P0AEP6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN42849 ; AAN42849 ; SF1236 .
    AAP16734 ; AAP16734 ; S1322 .
    GeneIDi 1024213.
    1077674.
    KEGGi sfl:SF1236.
    sfx:S1322.
    PATRICi 18703882. VBIShiFle31049_1445.

    Phylogenomic databases

    eggNOGi COG1210.
    HOGENOMi HOG000283477.
    KOi K00963.
    OMAi SICPKHV.
    OrthoDBi EOG6Z9B3V.

    Enzyme and pathway databases

    UniPathwayi UPA00030 .

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR005771. GalU_uridylyltTrfase_bac/arc.
    IPR005835. NTP_transferase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF00483. NTP_transferase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    TIGRFAMsi TIGR01099. galU. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Avirulence of rough mutants of Shigella flexneri: requirement of O antigen for correct unipolar localization of IcsA in the bacterial outer membrane."
      Sandlin R.C., Lampel K.A., Keasler S.P., Goldberg M.B., Stolzer A.L., Maurelli A.T.
      Infect. Immun. 63:229-237(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION OF ROLE IN LPS SYNTHESIS AND VIRULENCE, DISRUPTION PHENOTYPE.
      Strain: ATCC 700930 / 2457T / Serotype 2a.
    2. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
      Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
      , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
      Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 301 / Serotype 2a.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700930 / 2457T / Serotype 2a.

    Entry informationi

    Entry nameiGALU_SHIFL
    AccessioniPrimary (citable) accession number: P0AEP6
    Secondary accession number(s): P25520
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 65 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3