P0AEP6 (GALU_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UTP--glucose-1-phosphate uridylyltransferase
EC=2.7.7.9

Alternative name(s):
Alpha-D-glucosyl-1-phosphate uridylyltransferase
UDP-glucose pyrophosphorylase
Short name=UDPGP
Uridine diphosphoglucose pyrophosphorylase

Gene names

Name:	galU
Ordered Locus Names:	SF1236, S1322

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in stationary phase survival By similarity. Ref.1
Catalytic activity	UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.
Cofactor	Magnesium By similarity.
Pathway	Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Subunit structure	Homotetramer or homopentamer By similarity.
Disruption phenotype	A transposon mutant in galU (only the first 220 residues could be expressed) allows HeLa cell invasion but no cell spreading. Shows decreased secretion of IscA, a protein required for cell spreading. The lipopolysaccharide produced is of a size consistent with it containing only lipid A and a single core component. Ref.1
Sequence similarities	Belongs to the UDPGP type 2 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis Virulence
Ligand	Magnesium
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	UDP-glucose metabolic process Inferred from electronic annotation. Source: InterPro lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	UTP:glucose-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 302	301	UTP--glucose-1-phosphate uridylyltransferase		PRO_0000201363

Sequences

	Sequence		Length	Mass (Da)
	P0AEP6 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: ADEAEB3F6A1194CB	FASTA	302	32,942
	10 20 30 40 50 60 MAAINTKVKK AVIPVAGLGT RMLPATKAIP KEMLPLVDKP LIQYVVNECI AAGITEIVLV 70 80 90 100 110 120 THSSKNSIEN HFDTSFELEA MLEKRVKRQL LDEVQSICPP HVTIMQVRQG LAKGLGHAVL 130 140 150 160 170 180 CAHPVVGDEP VAVILPDVIL DEYESDLSQD NLAEMIRRFD ETGHSQIMVE PVADVTAYGV 190 200 210 220 230 240 VDCKGVELAP GESVPMVGVV EKPKADVAPS NLAIVGRYVL SADIWPLLAK TPPGAGDEIQ 250 260 270 280 290 300 LTDAIDMLIE KETVEAYHMK GKSHDCGNKL GYMQAFVEYG IRHNTLGTEF KAWLEEEMGI KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Avirulence of rough mutants of Shigella flexneri: requirement of O antigen for correct unipolar localization of IcsA in the bacterial outer membrane." Sandlin R.C., Lampel K.A., Keasler S.P., Goldberg M.B., Stolzer A.L., Maurelli A.T. Infect. Immun. 63:229-237(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION OF ROLE IN LPS SYNTHESIS AND VIRULENCE, DISRUPTION PHENOTYPE. Strain: ATCC 700930 / 2457T / Serotype 2a.
[2]	"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.
[3]	"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L32811 Genomic DNA. Translation: AAD15244.1. AE005674 Genomic DNA. Translation: AAN42849.1. AE014073 Genomic DNA. Translation: AAP16734.1.
RefSeq	NP_707142.1. NC_004337.2. NP_836927.1. NC_004741.1.
3D structure databases
ProteinModelPortal	P0AEP6.
SMR	P0AEP6. Positions 4-297.
ModBase	Search...
Protein-protein interaction databases
STRING	198214.SF1236.
Proteomic databases
PaxDb	P0AEP6.
PRIDE	P0AEP6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN42849; AAN42849; SF1236. AAP16734; AAP16734; S1322.
GeneID	1024213. 1077674.
KEGG	sfl:SF1236. sfx:S1322.
PATRIC	18703882. VBIShiFle31049_1445.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1210.
HOGENOM	HOG000283477.
KO	K00963.
OMA	HETHAST.
ProtClustDB	PRK13389.
Enzyme and pathway databases
UniPathway	UPA00030.
Family and domain databases
InterPro	IPR005771. GalU_uridylyltTrfase_bac/arc. IPR005835. NTP_transferase. [Graphical view]
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01099. galU. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GALU_SHIFL

Accession

Primary (citable) accession number: P0AEP6
Secondary accession number(s): P25520

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program