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P0AEP6

- GALU_SHIFL

UniProt

P0AEP6 - GALU_SHIFL

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Protein

UTP--glucose-1-phosphate uridylyltransferase

Gene

galU

Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

May play a role in stationary phase survival.By similarity

Catalytic activityi

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Cofactori

Mg2+By similarity

Pathwayi

GO - Molecular functioni

  1. UTP:glucose-1-phosphate uridylyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
  2. pathogenesis Source: UniProtKB-KW
  3. UDP-glucose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis, Virulence

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

UniPathwayiUPA00030.

Names & Taxonomyi

Protein namesi
Recommended name:
UTP--glucose-1-phosphate uridylyltransferase (EC:2.7.7.9)
Alternative name(s):
Alpha-D-glucosyl-1-phosphate uridylyltransferase
UDP-glucose pyrophosphorylase
Short name:
UDPGP
Uridine diphosphoglucose pyrophosphorylase
Gene namesi
Name:galU
Ordered Locus Names:SF1236, S1322
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

Pathology & Biotechi

Disruption phenotypei

A transposon mutant in galU (only the first 220 residues could be expressed) allows HeLa cell invasion but no cell spreading. Shows decreased secretion of IscA, a protein required for cell spreading. The lipopolysaccharide produced is of a size consistent with it containing only lipid A and a single core component.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 302301UTP--glucose-1-phosphate uridylyltransferasePRO_0000201363Add
BLAST

Proteomic databases

PaxDbiP0AEP6.
PRIDEiP0AEP6.

Interactioni

Subunit structurei

Homotetramer or homopentamer.By similarity

Protein-protein interaction databases

STRINGi198214.SF1236.

Structurei

3D structure databases

ProteinModelPortaliP0AEP6.
SMRiP0AEP6. Positions 4-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDPGP type 2 family.Curated

Phylogenomic databases

eggNOGiCOG1210.
HOGENOMiHOG000283477.
KOiK00963.
OMAiSICPKHV.
OrthoDBiEOG6Z9B3V.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005771. GalU_uridylyltTrfase_bac/arc.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01099. galU. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEP6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAINTKVKK AVIPVAGLGT RMLPATKAIP KEMLPLVDKP LIQYVVNECI
60 70 80 90 100
AAGITEIVLV THSSKNSIEN HFDTSFELEA MLEKRVKRQL LDEVQSICPP
110 120 130 140 150
HVTIMQVRQG LAKGLGHAVL CAHPVVGDEP VAVILPDVIL DEYESDLSQD
160 170 180 190 200
NLAEMIRRFD ETGHSQIMVE PVADVTAYGV VDCKGVELAP GESVPMVGVV
210 220 230 240 250
EKPKADVAPS NLAIVGRYVL SADIWPLLAK TPPGAGDEIQ LTDAIDMLIE
260 270 280 290 300
KETVEAYHMK GKSHDCGNKL GYMQAFVEYG IRHNTLGTEF KAWLEEEMGI

KK
Length:302
Mass (Da):32,942
Last modified:January 23, 2007 - v2
Checksum:iADEAEB3F6A1194CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32811 Genomic DNA. Translation: AAD15244.1.
AE005674 Genomic DNA. Translation: AAN42849.1.
AE014073 Genomic DNA. Translation: AAP16734.1.
RefSeqiNP_707142.1. NC_004337.2.
NP_836927.1. NC_004741.1.

Genome annotation databases

EnsemblBacteriaiAAN42849; AAN42849; SF1236.
AAP16734; AAP16734; S1322.
GeneIDi1024213.
1077674.
KEGGisfl:SF1236.
sfx:S1322.
PATRICi18703882. VBIShiFle31049_1445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32811 Genomic DNA. Translation: AAD15244.1 .
AE005674 Genomic DNA. Translation: AAN42849.1 .
AE014073 Genomic DNA. Translation: AAP16734.1 .
RefSeqi NP_707142.1. NC_004337.2.
NP_836927.1. NC_004741.1.

3D structure databases

ProteinModelPortali P0AEP6.
SMRi P0AEP6. Positions 4-297.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198214.SF1236.

Proteomic databases

PaxDbi P0AEP6.
PRIDEi P0AEP6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN42849 ; AAN42849 ; SF1236 .
AAP16734 ; AAP16734 ; S1322 .
GeneIDi 1024213.
1077674.
KEGGi sfl:SF1236.
sfx:S1322.
PATRICi 18703882. VBIShiFle31049_1445.

Phylogenomic databases

eggNOGi COG1210.
HOGENOMi HOG000283477.
KOi K00963.
OMAi SICPKHV.
OrthoDBi EOG6Z9B3V.

Enzyme and pathway databases

UniPathwayi UPA00030 .

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR005771. GalU_uridylyltTrfase_bac/arc.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
Pfami PF00483. NTP_transferase. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
TIGRFAMsi TIGR01099. galU. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Avirulence of rough mutants of Shigella flexneri: requirement of O antigen for correct unipolar localization of IcsA in the bacterial outer membrane."
    Sandlin R.C., Lampel K.A., Keasler S.P., Goldberg M.B., Stolzer A.L., Maurelli A.T.
    Infect. Immun. 63:229-237(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION OF ROLE IN LPS SYNTHESIS AND VIRULENCE, DISRUPTION PHENOTYPE.
    Strain: ATCC 700930 / 2457T / Serotype 2a.
  2. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiGALU_SHIFL
AccessioniPrimary (citable) accession number: P0AEP6
Secondary accession number(s): P25520
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3