P0AEP5 (GALU_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UTP--glucose-1-phosphate uridylyltransferase
EC=2.7.7.9

Alternative name(s):
Alpha-D-glucosyl-1-phosphate uridylyltransferase
UDP-glucose pyrophosphorylase
Short name=UDPGP
Uridine diphosphoglucose pyrophosphorylase

Gene names

Name:	galU
Ordered Locus Names:	Z2012, ECs1738

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May play a role in stationary phase survival By similarity.
Catalytic activity	UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.
Cofactor	Magnesium By similarity.
Subunit structure	Homotetramer or homopentamer By similarity.
Sequence similarities	Belongs to the UDPGP type 2 family.

Ontologies

Keywords
Ligand	Magnesium
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-glucose metabolic process Inferred from electronic annotation. Source: InterPro biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	UTP:glucose-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 302	301	UTP--glucose-1-phosphate uridylyltransferase		PRO_0000201355

Sequences

	Sequence		Length	Mass (Da)
	P0AEP5 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: ADEAEB3F6A1194CB	FASTA	302	32,942
	10 20 30 40 50 60 MAAINTKVKK AVIPVAGLGT RMLPATKAIP KEMLPLVDKP LIQYVVNECI AAGITEIVLV 70 80 90 100 110 120 THSSKNSIEN HFDTSFELEA MLEKRVKRQL LDEVQSICPP HVTIMQVRQG LAKGLGHAVL 130 140 150 160 170 180 CAHPVVGDEP VAVILPDVIL DEYESDLSQD NLAEMIRRFD ETGHSQIMVE PVADVTAYGV 190 200 210 220 230 240 VDCKGVELAP GESVPMVGVV EKPKADVAPS NLAIVGRYVL SADIWPLLAK TPPGAGDEIQ 250 260 270 280 290 300 LTDAIDMLIE KETVEAYHMK GKSHDCGNKL GYMQAFVEYG IRHNTLGTEF KAWLEEEMGI KK

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG56093.1. BA000007 Genomic DNA. Translation: BAB35161.1.
PIR	A85704. B90846.
RefSeq	NP_287481.1. NC_002655.2. NP_309765.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P0AEP5.
SMR	P0AEP5. Positions 4-297.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000024959; EBESCP00000023852; EBESCG00000024013. EBESCT00000058884; EBESCP00000056712; EBESCG00000057932.
GeneID	913114. 960488.
GenomeReviews	Gene locus Z2012 in contig AE005174_GR. Gene locus ECs1738 in contig BA000007_GR.
KEGG	ece:Z2012. ecs:ECs1738.
PATRIC	18352738. VBIEscCol44059_1592.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000009639.
HOGENOM	HBG688195.
OMA	TILGVQT.
ProtClustDB	PRK13389.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS1738-MONOMER.
Family and domain databases
InterPro	IPR005771. GalU_uridylyltTrfase_bac/arc. IPR005835. NTP_transferase. [Graphical view]
KO	K00963.
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01099. GalU. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GALU_ECO57

Accession

Primary (citable) accession number: P0AEP5
Secondary accession number(s): P25520

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program