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P0AEP3 (GALU_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UTP--glucose-1-phosphate uridylyltransferase

EC=2.7.7.9
Alternative name(s):
Alpha-D-glucosyl-1-phosphate uridylyltransferase
UDP-glucose pyrophosphorylase
Short name=UDPGP
Uridine diphosphoglucose pyrophosphorylase
Gene names
Name:galU
Synonyms:ychD
Ordered Locus Names:b1236, JW1224
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in stationary phase survival.

Catalytic activity

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Cofactor

Magnesium.

Subunit structure

Homotetramer or homopentamer.

Sequence similarities

Belongs to the UDPGP type 2 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

purUP370511EBI-909038,EBI-1122931

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2 Ref.7
Chain2 – 302301UTP--glucose-1-phosphate uridylyltransferase
PRO_0000201354

Secondary structure

............................................................ 302
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AEP3 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: ADEAEB3F6A1194CB

FASTA30232,942
        10         20         30         40         50         60 
MAAINTKVKK AVIPVAGLGT RMLPATKAIP KEMLPLVDKP LIQYVVNECI AAGITEIVLV 

        70         80         90        100        110        120 
THSSKNSIEN HFDTSFELEA MLEKRVKRQL LDEVQSICPP HVTIMQVRQG LAKGLGHAVL 

       130        140        150        160        170        180 
CAHPVVGDEP VAVILPDVIL DEYESDLSQD NLAEMIRRFD ETGHSQIMVE PVADVTAYGV 

       190        200        210        220        230        240 
VDCKGVELAP GESVPMVGVV EKPKADVAPS NLAIVGRYVL SADIWPLLAK TPPGAGDEIQ 

       250        260        270        280        290        300 
LTDAIDMLIE KETVEAYHMK GKSHDCGNKL GYMQAFVEYG IRHNTLGTEF KAWLEEEMGI 


KK 

« Hide

References

« Hide 'large scale' references
[1]"Multicopy suppression: an approach to understanding intracellular functioning of the protein export system."
Ueguchi C., Ito K.
J. Bacteriol. 174:1454-1461(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"UTP: alpha-D-glucose-1-phosphate uridylyltransferase of Escherichia coli: isolation and DNA sequence of the galU gene and purification of the enzyme."
Weissborn A.C., Liu Q., Rumley M.K., Kennedy E.P.
J. Bacteriol. 176:2611-2618(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11, CHARACTERIZATION.
Strain: K12.
[3]"Overproduction and characterization of recombinant UDP-glucose pyrophosphorylase from Escherichia coli K-12."
Hossain S.A., Tanizawa K., Kazuta Y., Fukui T.
J. Biochem. 115:965-972(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: K12.
[4]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[6]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-18.
Strain: K12 / EMG2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59940 Genomic DNA. Translation: CAA42564.1.
M98830 Unassigned DNA. Translation: AAA20118.1.
U00096 Genomic DNA. Translation: AAC74318.1.
AP009048 Genomic DNA. Translation: BAA36104.1.
PIRJC2265. G64870.
RefSeqNP_415752.1. NC_000913.3.
YP_489504.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2E3DX-ray1.95A/B/C/D1-302[»]
ProteinModelPortalP0AEP3.
SMRP0AEP3. Positions 4-297.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-35950N.
IntActP0AEP3. 3 interactions.
STRING511145.b1236.

Proteomic databases

PaxDbP0AEP3.
PRIDEP0AEP3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74318; AAC74318; b1236.
BAA36104; BAA36104; BAA36104.
GeneID12931127.
945730.
KEGGecj:Y75_p1209.
eco:b1236.
PATRIC32117728. VBIEscCol129921_1284.

Organism-specific databases

EchoBASEEB1295.
EcoGeneEG11319. galU.

Phylogenomic databases

eggNOGCOG1210.
HOGENOMHOG000283477.
KOK00963.
OMADIHFELE.
OrthoDBEOG6Z9B3V.
PhylomeDBP0AEP3.
ProtClustDBPRK13389.

Enzyme and pathway databases

BioCycEcoCyc:GLUC1PURIDYLTRANS-MONOMER.
ECOL316407:JW1224-MONOMER.
MetaCyc:GLUC1PURIDYLTRANS-MONOMER.
SABIO-RKP0AEP3.

Gene expression databases

GenevestigatorP0AEP3.

Family and domain databases

InterProIPR005771. GalU_uridylyltTrfase_bac/arc.
IPR005835. NTP_transferase.
[Graphical view]
PfamPF00483. NTP_transferase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01099. galU. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0AEP3.
PROP0AEP3.

Entry information

Entry nameGALU_ECOLI
AccessionPrimary (citable) accession number: P0AEP3
Secondary accession number(s): P25520
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene