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P0AEP3

- GALU_ECOLI

UniProt

P0AEP3 - GALU_ECOLI

Protein

UTP--glucose-1-phosphate uridylyltransferase

Gene

galU

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    May play a role in stationary phase survival.

    Catalytic activityi

    UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. magnesium ion binding Source: EcoCyc
    2. UTP:glucose-1-phosphate uridylyltransferase activity Source: EcoCyc

    GO - Biological processi

    1. colanic acid biosynthetic process Source: EcoCyc
    2. galactose catabolic process via UDP-galactose Source: EcoCyc
    3. lipopolysaccharide biosynthetic process Source: EcoCyc
    4. UDP-glucose metabolic process Source: EcoCyc

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciEcoCyc:GLUC1PURIDYLTRANS-MONOMER.
    ECOL316407:JW1224-MONOMER.
    MetaCyc:GLUC1PURIDYLTRANS-MONOMER.
    SABIO-RKP0AEP3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UTP--glucose-1-phosphate uridylyltransferase (EC:2.7.7.9)
    Alternative name(s):
    Alpha-D-glucosyl-1-phosphate uridylyltransferase
    UDP-glucose pyrophosphorylase
    Short name:
    UDPGP
    Uridine diphosphoglucose pyrophosphorylase
    Gene namesi
    Name:galU
    Synonyms:ychD
    Ordered Locus Names:b1236, JW1224
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11319. galU.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 302301UTP--glucose-1-phosphate uridylyltransferasePRO_0000201354Add
    BLAST

    Proteomic databases

    PaxDbiP0AEP3.
    PRIDEiP0AEP3.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AEP3.

    Interactioni

    Subunit structurei

    Homotetramer or homopentamer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    purUP370511EBI-909038,EBI-1122931

    Protein-protein interaction databases

    DIPiDIP-35950N.
    IntActiP0AEP3. 3 interactions.
    STRINGi511145.b1236.

    Structurei

    Secondary structure

    1
    302
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi10 – 145
    Helixi20 – 223
    Turni23 – 264
    Beta strandi27 – 293
    Helixi31 – 333
    Helixi41 – 5111
    Beta strandi56 – 616
    Helixi63 – 653
    Helixi66 – 727
    Helixi76 – 827
    Helixi89 – 968
    Beta strandi103 – 1086
    Helixi115 – 1217
    Helixi123 – 1264
    Beta strandi131 – 1344
    Beta strandi138 – 1403
    Turni147 – 1493
    Helixi152 – 16211
    Beta strandi165 – 1717
    Helixi175 – 1773
    Beta strandi178 – 1825
    Beta strandi193 – 1964
    Beta strandi198 – 2025
    Turni205 – 2073
    Beta strandi211 – 22010
    Helixi224 – 2285
    Helixi241 – 25111
    Beta strandi254 – 2585
    Beta strandi263 – 2653
    Helixi269 – 28214
    Turni284 – 2863
    Helixi287 – 29711

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2E3DX-ray1.95A/B/C/D1-302[»]
    ProteinModelPortaliP0AEP3.
    SMRiP0AEP3. Positions 4-297.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AEP3.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UDPGP type 2 family.Curated

    Phylogenomic databases

    eggNOGiCOG1210.
    HOGENOMiHOG000283477.
    KOiK00963.
    OMAiSICPKHV.
    OrthoDBiEOG6Z9B3V.
    PhylomeDBiP0AEP3.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR005771. GalU_uridylyltTrfase_bac/arc.
    IPR005835. NTP_transferase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF00483. NTP_transferase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.
    TIGRFAMsiTIGR01099. galU. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AEP3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAINTKVKK AVIPVAGLGT RMLPATKAIP KEMLPLVDKP LIQYVVNECI    50
    AAGITEIVLV THSSKNSIEN HFDTSFELEA MLEKRVKRQL LDEVQSICPP 100
    HVTIMQVRQG LAKGLGHAVL CAHPVVGDEP VAVILPDVIL DEYESDLSQD 150
    NLAEMIRRFD ETGHSQIMVE PVADVTAYGV VDCKGVELAP GESVPMVGVV 200
    EKPKADVAPS NLAIVGRYVL SADIWPLLAK TPPGAGDEIQ LTDAIDMLIE 250
    KETVEAYHMK GKSHDCGNKL GYMQAFVEYG IRHNTLGTEF KAWLEEEMGI 300
    KK 302
    Length:302
    Mass (Da):32,942
    Last modified:January 23, 2007 - v2
    Checksum:iADEAEB3F6A1194CB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59940 Genomic DNA. Translation: CAA42564.1.
    M98830 Unassigned DNA. Translation: AAA20118.1.
    U00096 Genomic DNA. Translation: AAC74318.1.
    AP009048 Genomic DNA. Translation: BAA36104.1.
    PIRiG64870. JC2265.
    RefSeqiNP_415752.1. NC_000913.3.
    YP_489504.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74318; AAC74318; b1236.
    BAA36104; BAA36104; BAA36104.
    GeneIDi12931127.
    945730.
    KEGGiecj:Y75_p1209.
    eco:b1236.
    PATRICi32117728. VBIEscCol129921_1284.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59940 Genomic DNA. Translation: CAA42564.1 .
    M98830 Unassigned DNA. Translation: AAA20118.1 .
    U00096 Genomic DNA. Translation: AAC74318.1 .
    AP009048 Genomic DNA. Translation: BAA36104.1 .
    PIRi G64870. JC2265.
    RefSeqi NP_415752.1. NC_000913.3.
    YP_489504.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2E3D X-ray 1.95 A/B/C/D 1-302 [» ]
    ProteinModelPortali P0AEP3.
    SMRi P0AEP3. Positions 4-297.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35950N.
    IntActi P0AEP3. 3 interactions.
    STRINGi 511145.b1236.

    Proteomic databases

    PaxDbi P0AEP3.
    PRIDEi P0AEP3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74318 ; AAC74318 ; b1236 .
    BAA36104 ; BAA36104 ; BAA36104 .
    GeneIDi 12931127.
    945730.
    KEGGi ecj:Y75_p1209.
    eco:b1236.
    PATRICi 32117728. VBIEscCol129921_1284.

    Organism-specific databases

    EchoBASEi EB1295.
    EcoGenei EG11319. galU.

    Phylogenomic databases

    eggNOGi COG1210.
    HOGENOMi HOG000283477.
    KOi K00963.
    OMAi SICPKHV.
    OrthoDBi EOG6Z9B3V.
    PhylomeDBi P0AEP3.

    Enzyme and pathway databases

    BioCyci EcoCyc:GLUC1PURIDYLTRANS-MONOMER.
    ECOL316407:JW1224-MONOMER.
    MetaCyc:GLUC1PURIDYLTRANS-MONOMER.
    SABIO-RK P0AEP3.

    Miscellaneous databases

    EvolutionaryTracei P0AEP3.
    PROi P0AEP3.

    Gene expression databases

    Genevestigatori P0AEP3.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR005771. GalU_uridylyltTrfase_bac/arc.
    IPR005835. NTP_transferase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF00483. NTP_transferase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    TIGRFAMsi TIGR01099. galU. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Multicopy suppression: an approach to understanding intracellular functioning of the protein export system."
      Ueguchi C., Ito K.
      J. Bacteriol. 174:1454-1461(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "UTP: alpha-D-glucose-1-phosphate uridylyltransferase of Escherichia coli: isolation and DNA sequence of the galU gene and purification of the enzyme."
      Weissborn A.C., Liu Q., Rumley M.K., Kennedy E.P.
      J. Bacteriol. 176:2611-2618(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11, CHARACTERIZATION.
      Strain: K12.
    3. "Overproduction and characterization of recombinant UDP-glucose pyrophosphorylase from Escherichia coli K-12."
      Hossain S.A., Tanizawa K., Kazuta Y., Fukui T.
      J. Biochem. 115:965-972(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: K12.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    7. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
      Link A.J., Robison K., Church G.M.
      Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-18.
      Strain: K12 / EMG2.

    Entry informationi

    Entry nameiGALU_ECOLI
    AccessioniPrimary (citable) accession number: P0AEP3
    Secondary accession number(s): P25520
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3