UTP--glucose-1-phosphate uridylyltransferase - Escherichia coli (strain K12)

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P0AEP3 (GALU_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 58. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
UTP--glucose-1-phosphate uridylyltransferase
EC=2.7.7.9

Alternative name(s):
Alpha-D-glucosyl-1-phosphate uridylyltransferase
UDP-glucose pyrophosphorylase
Short name=UDPGP
Uridine diphosphoglucose pyrophosphorylase

Gene names

Name:	galU
Synonyms:	ychD
Ordered Locus Names:	b1236, JW1224

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in stationary phase survival.
Catalytic activity	UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.
Cofactor	Magnesium.
Subunit structure	Homotetramer or homopentamer.
Sequence similarities	Belongs to the UDPGP type 2 family.

Ontologies

Keywords
Ligand	Magnesium
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	UDP-glucose metabolic process Inferred from electronic annotation. Source: InterPro biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB
Molecular function	UTP:glucose-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
purU	P37051	1	EBI-909038,EBI-1122931

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.2 Ref.7

Chain

2 – 302

301

UTP--glucose-1-phosphate uridylyltransferase

PRO_0000201354

Secondary structure

302

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0AEP3 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: ADEAEB3F6A1194CB
Show »

FASTA

302

32,942

        10         20         30         40         50         60 
MAAINTKVKK AVIPVAGLGT RMLPATKAIP KEMLPLVDKP LIQYVVNECI AAGITEIVLV 

        70         80         90        100        110        120 
THSSKNSIEN HFDTSFELEA MLEKRVKRQL LDEVQSICPP HVTIMQVRQG LAKGLGHAVL 

       130        140        150        160        170        180 
CAHPVVGDEP VAVILPDVIL DEYESDLSQD NLAEMIRRFD ETGHSQIMVE PVADVTAYGV 

       190        200        210        220        230        240 
VDCKGVELAP GESVPMVGVV EKPKADVAPS NLAIVGRYVL SADIWPLLAK TPPGAGDEIQ 

       250        260        270        280        290        300 
LTDAIDMLIE KETVEAYHMK GKSHDCGNKL GYMQAFVEYG IRHNTLGTEF KAWLEEEMGI 


KK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multicopy suppression: an approach to understanding intracellular functioning of the protein export system." Ueguchi C., Ito K. J. Bacteriol. 174:1454-1461(1992) [PubMed: 1537791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"UTP: alpha-D-glucose-1-phosphate uridylyltransferase of Escherichia coli: isolation and DNA sequence of the galU gene and purification of the enzyme." Weissborn A.C., Liu Q., Rumley M.K., Kennedy E.P. J. Bacteriol. 176:2611-2618(1994) [PubMed: 8169209] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11, CHARACTERIZATION. Strain: K12.
[3]	"Overproduction and characterization of recombinant UDP-glucose pyrophosphorylase from Escherichia coli K-12." Hossain S.A., Tanizawa K., Kazuta Y., Fukui T. J. Biochem. 115:965-972(1994) [PubMed: 7961613] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: K12.
[4]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-18. Strain: K12 / EMG2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X59940 Genomic DNA. Translation: CAA42564.1.
M98830 Unassigned DNA. Translation: AAA20118.1.
U00096 Genomic DNA. Translation: AAC74318.1.
AP009048 Genomic DNA. Translation: BAA36104.1.

PIR

JC2265. G64870.

RefSeq

NP_415752.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2E3D	X-ray	1.95	A/B/C/D	1-302	[»]

ProteinModelPortal

P0AEP3.

SMR

P0AEP3. Positions 4-297.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35950N.

IntAct

P0AEP3. 5 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002632; EBESCP00000002632; EBESCG00000002147.
EBESCT00000016774; EBESCP00000016065; EBESCG00000015833.

GeneID

945730.

GenomeReviews

Gene locus JW1224 in contig AP009048_GR.
Gene locus b1236 in contig U00096_GR.

KEGG

ecj:JW1224.
eco:b1236.

PATRIC

32117728. VBIEscCol129921_1284.

Organism-specific databases

EchoBASE

EB1295.

EcoGene

EG11319. galU.

Phylogenomic databases

eggNOG

COG1210.

GeneTree

EBGT00050000009639.

HOGENOM

HBG688195.

OMA

TILGVQT.

PhylomeDB

P0AEP3.

ProtClustDB

PRK13389.

Enzyme and pathway databases

BioCyc

EcoCyc:GLUC1PURIDYLTRANS-MONOMER.
MetaCyc:GLUC1PURIDYLTRANS-MONOMER.

Gene expression databases

Genevestigator

P0AEP3.

Family and domain databases

InterPro

IPR005771. GalU_uridylyltTrfase_bac/arc.
IPR005835. NTP_transferase.
[Graphical view]

K00963.

Pfam

PF00483. NTP_transferase. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01099. GalU. 1 hit.

ProtoNet

Search...

Entry information

Entry name

GALU_ECOLI

Accession

Primary (citable) accession number: P0AEP3
Secondary accession number(s): P25520

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents