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P0AEP3

- GALU_ECOLI

UniProt

P0AEP3 - GALU_ECOLI

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Protein
UTP--glucose-1-phosphate uridylyltransferase
Gene
galU, ychD, b1236, JW1224
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May play a role in stationary phase survival.

Catalytic activityi

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Cofactori

Magnesium.

GO - Molecular functioni

  1. UTP:glucose-1-phosphate uridylyltransferase activity Source: EcoCyc
  2. magnesium ion binding Source: EcoCyc

GO - Biological processi

  1. UDP-glucose metabolic process Source: EcoCyc
  2. colanic acid biosynthetic process Source: EcoCyc
  3. galactose catabolic process via UDP-galactose Source: EcoCyc
  4. lipopolysaccharide biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciEcoCyc:GLUC1PURIDYLTRANS-MONOMER.
ECOL316407:JW1224-MONOMER.
MetaCyc:GLUC1PURIDYLTRANS-MONOMER.
SABIO-RKP0AEP3.

Names & Taxonomyi

Protein namesi
Recommended name:
UTP--glucose-1-phosphate uridylyltransferase (EC:2.7.7.9)
Alternative name(s):
Alpha-D-glucosyl-1-phosphate uridylyltransferase
UDP-glucose pyrophosphorylase
Short name:
UDPGP
Uridine diphosphoglucose pyrophosphorylase
Gene namesi
Name:galU
Synonyms:ychD
Ordered Locus Names:b1236, JW1224
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11319. galU.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 302301UTP--glucose-1-phosphate uridylyltransferase
PRO_0000201354Add
BLAST

Proteomic databases

PaxDbiP0AEP3.
PRIDEiP0AEP3.

Expressioni

Gene expression databases

GenevestigatoriP0AEP3.

Interactioni

Subunit structurei

Homotetramer or homopentamer.

Binary interactionsi

WithEntry#Exp.IntActNotes
purUP370511EBI-909038,EBI-1122931

Protein-protein interaction databases

DIPiDIP-35950N.
IntActiP0AEP3. 3 interactions.
STRINGi511145.b1236.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 145
Helixi20 – 223
Turni23 – 264
Beta strandi27 – 293
Helixi31 – 333
Helixi41 – 5111
Beta strandi56 – 616
Helixi63 – 653
Helixi66 – 727
Helixi76 – 827
Helixi89 – 968
Beta strandi103 – 1086
Helixi115 – 1217
Helixi123 – 1264
Beta strandi131 – 1344
Beta strandi138 – 1403
Turni147 – 1493
Helixi152 – 16211
Beta strandi165 – 1717
Helixi175 – 1773
Beta strandi178 – 1825
Beta strandi193 – 1964
Beta strandi198 – 2025
Turni205 – 2073
Beta strandi211 – 22010
Helixi224 – 2285
Helixi241 – 25111
Beta strandi254 – 2585
Beta strandi263 – 2653
Helixi269 – 28214
Turni284 – 2863
Helixi287 – 29711

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E3DX-ray1.95A/B/C/D1-302[»]
ProteinModelPortaliP0AEP3.
SMRiP0AEP3. Positions 4-297.

Miscellaneous databases

EvolutionaryTraceiP0AEP3.

Family & Domainsi

Sequence similaritiesi

Belongs to the UDPGP type 2 family.

Phylogenomic databases

eggNOGiCOG1210.
HOGENOMiHOG000283477.
KOiK00963.
OMAiSICPKHV.
OrthoDBiEOG6Z9B3V.
PhylomeDBiP0AEP3.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005771. GalU_uridylyltTrfase_bac/arc.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01099. galU. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEP3-1 [UniParc]FASTAAdd to Basket

« Hide

MAAINTKVKK AVIPVAGLGT RMLPATKAIP KEMLPLVDKP LIQYVVNECI    50
AAGITEIVLV THSSKNSIEN HFDTSFELEA MLEKRVKRQL LDEVQSICPP 100
HVTIMQVRQG LAKGLGHAVL CAHPVVGDEP VAVILPDVIL DEYESDLSQD 150
NLAEMIRRFD ETGHSQIMVE PVADVTAYGV VDCKGVELAP GESVPMVGVV 200
EKPKADVAPS NLAIVGRYVL SADIWPLLAK TPPGAGDEIQ LTDAIDMLIE 250
KETVEAYHMK GKSHDCGNKL GYMQAFVEYG IRHNTLGTEF KAWLEEEMGI 300
KK 302
Length:302
Mass (Da):32,942
Last modified:January 23, 2007 - v2
Checksum:iADEAEB3F6A1194CB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59940 Genomic DNA. Translation: CAA42564.1.
M98830 Unassigned DNA. Translation: AAA20118.1.
U00096 Genomic DNA. Translation: AAC74318.1.
AP009048 Genomic DNA. Translation: BAA36104.1.
PIRiG64870. JC2265.
RefSeqiNP_415752.1. NC_000913.3.
YP_489504.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74318; AAC74318; b1236.
BAA36104; BAA36104; BAA36104.
GeneIDi12931127.
945730.
KEGGiecj:Y75_p1209.
eco:b1236.
PATRICi32117728. VBIEscCol129921_1284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59940 Genomic DNA. Translation: CAA42564.1 .
M98830 Unassigned DNA. Translation: AAA20118.1 .
U00096 Genomic DNA. Translation: AAC74318.1 .
AP009048 Genomic DNA. Translation: BAA36104.1 .
PIRi G64870. JC2265.
RefSeqi NP_415752.1. NC_000913.3.
YP_489504.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2E3D X-ray 1.95 A/B/C/D 1-302 [» ]
ProteinModelPortali P0AEP3.
SMRi P0AEP3. Positions 4-297.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-35950N.
IntActi P0AEP3. 3 interactions.
STRINGi 511145.b1236.

Proteomic databases

PaxDbi P0AEP3.
PRIDEi P0AEP3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74318 ; AAC74318 ; b1236 .
BAA36104 ; BAA36104 ; BAA36104 .
GeneIDi 12931127.
945730.
KEGGi ecj:Y75_p1209.
eco:b1236.
PATRICi 32117728. VBIEscCol129921_1284.

Organism-specific databases

EchoBASEi EB1295.
EcoGenei EG11319. galU.

Phylogenomic databases

eggNOGi COG1210.
HOGENOMi HOG000283477.
KOi K00963.
OMAi SICPKHV.
OrthoDBi EOG6Z9B3V.
PhylomeDBi P0AEP3.

Enzyme and pathway databases

BioCyci EcoCyc:GLUC1PURIDYLTRANS-MONOMER.
ECOL316407:JW1224-MONOMER.
MetaCyc:GLUC1PURIDYLTRANS-MONOMER.
SABIO-RK P0AEP3.

Miscellaneous databases

EvolutionaryTracei P0AEP3.
PROi P0AEP3.

Gene expression databases

Genevestigatori P0AEP3.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR005771. GalU_uridylyltTrfase_bac/arc.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
Pfami PF00483. NTP_transferase. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
TIGRFAMsi TIGR01099. galU. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Multicopy suppression: an approach to understanding intracellular functioning of the protein export system."
    Ueguchi C., Ito K.
    J. Bacteriol. 174:1454-1461(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "UTP: alpha-D-glucose-1-phosphate uridylyltransferase of Escherichia coli: isolation and DNA sequence of the galU gene and purification of the enzyme."
    Weissborn A.C., Liu Q., Rumley M.K., Kennedy E.P.
    J. Bacteriol. 176:2611-2618(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11, CHARACTERIZATION.
    Strain: K12.
  3. "Overproduction and characterization of recombinant UDP-glucose pyrophosphorylase from Escherichia coli K-12."
    Hossain S.A., Tanizawa K., Kazuta Y., Fukui T.
    J. Biochem. 115:965-972(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: K12.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-18.
    Strain: K12 / EMG2.

Entry informationi

Entry nameiGALU_ECOLI
AccessioniPrimary (citable) accession number: P0AEP3
Secondary accession number(s): P25520
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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