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Protein

L-fucose mutarotase

Gene

fucU

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the anomeric conversion of L-fucose. It also exhibits a pyranase activity for D-ribose.1 Publication

Catalytic activityi

Alpha-L-fucose = beta-L-fucose.

Pathwayi: L-fucose metabolism

This protein is involved in the pathway L-fucose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway L-fucose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei22Proton donorBy similarity1
Binding sitei30SubstrateBy similarity1
Binding sitei107SubstrateBy similarity1

GO - Molecular functioni

  • fucose binding Source: MGI
  • racemase and epimerase activity, acting on carbohydrates and derivatives Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism, Fucose metabolism

Enzyme and pathway databases

BioCyciEcoCyc:EG10355-MONOMER.
ECOL316407:JW2775-MONOMER.
MetaCyc:EG10355-MONOMER.
BRENDAi5.1.3.29. 2026.
UniPathwayiUPA00956.

Names & Taxonomyi

Protein namesi
Recommended name:
L-fucose mutarotase (EC:5.1.3.29)
Alternative name(s):
Fucose 1-epimerase
Type-2 mutarotase
Gene namesi
Name:fucU
Ordered Locus Names:b2804, JW2775
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10355. fucU.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000873801 – 140L-fucose mutarotaseAdd BLAST140

Proteomic databases

EPDiP0AEN8.
PaxDbiP0AEN8.
PRIDEiP0AEN8.

Interactioni

Subunit structurei

Homodecamer.1 Publication

Protein-protein interaction databases

BioGridi4262305. 16 interactors.
DIPiDIP-47990N.
IntActiP0AEN8. 5 interactors.
MINTiMINT-1243432.
STRINGi511145.b2804.

Structurei

Secondary structure

1140
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 19Combined sources9
Beta strandi25 – 29Combined sources5
Helixi35 – 38Combined sources4
Beta strandi39 – 45Combined sources7
Helixi50 – 57Combined sources8
Turni58 – 60Combined sources3
Beta strandi65 – 67Combined sources3
Beta strandi70 – 73Combined sources4
Helixi83 – 92Combined sources10
Beta strandi103 – 105Combined sources3
Helixi107 – 114Combined sources8
Beta strandi117 – 123Combined sources7
Beta strandi132 – 136Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WCVX-ray1.90A/B/C/D/E/F/G/H/I/J1-140[»]
ProteinModelPortaliP0AEN8.
SMRiP0AEN8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEN8.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 131Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105NWF. Bacteria.
COG4154. LUCA.
HOGENOMiHOG000219041.
InParanoidiP0AEN8.
KOiK02431.
OMAiADLNFPA.
PhylomeDBiP0AEN8.

Family and domain databases

Gene3Di3.40.1650.10. 1 hit.
HAMAPiMF_01662. L_fucose_rotase. 1 hit.
InterProiIPR023751. L-fucose_mutarotase.
IPR023750. RbsD-like.
IPR007721. RbsD_FucU.
[Graphical view]
PfamiPF05025. RbsD_FucU. 1 hit.
[Graphical view]
SUPFAMiSSF102546. SSF102546. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AEN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKTISPLIS PELLKVLAEM GHGDEIIFSD AHFPAHSMGP QVIRADGLLV
60 70 80 90 100
SDLLQAIIPL FELDSYAPPL VMMAAVEGDT LDPEVERRYR NALSLQAPCP
110 120 130 140
DIIRINRFAF YERAQKAFAI VITGERAKYG NILLKKGVTP
Length:140
Mass (Da):15,473
Last modified:December 20, 2005 - v1
Checksum:i55D304F7BF17B8F4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106 – 140NRFAF…KGVTP → IVLRFMNGRKKPLRSLSQAN ERSTGIFF in CAA33129 (PubMed:2664711).CuratedAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15025 Genomic DNA. Translation: CAA33129.1.
U29581 Genomic DNA. Translation: AAB40454.1.
U00096 Genomic DNA. Translation: AAC75846.1.
AP009048 Genomic DNA. Translation: BAE76876.1.
PIRiH65062. Q4ECKR.
RefSeqiNP_417284.1. NC_000913.3.
WP_000920840.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75846; AAC75846; b2804.
BAE76876; BAE76876; BAE76876.
GeneIDi945842.
KEGGiecj:JW2775.
eco:b2804.
PATRICi32121024. VBIEscCol129921_2904.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15025 Genomic DNA. Translation: CAA33129.1.
U29581 Genomic DNA. Translation: AAB40454.1.
U00096 Genomic DNA. Translation: AAC75846.1.
AP009048 Genomic DNA. Translation: BAE76876.1.
PIRiH65062. Q4ECKR.
RefSeqiNP_417284.1. NC_000913.3.
WP_000920840.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WCVX-ray1.90A/B/C/D/E/F/G/H/I/J1-140[»]
ProteinModelPortaliP0AEN8.
SMRiP0AEN8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262305. 16 interactors.
DIPiDIP-47990N.
IntActiP0AEN8. 5 interactors.
MINTiMINT-1243432.
STRINGi511145.b2804.

Proteomic databases

EPDiP0AEN8.
PaxDbiP0AEN8.
PRIDEiP0AEN8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75846; AAC75846; b2804.
BAE76876; BAE76876; BAE76876.
GeneIDi945842.
KEGGiecj:JW2775.
eco:b2804.
PATRICi32121024. VBIEscCol129921_2904.

Organism-specific databases

EchoBASEiEB0350.
EcoGeneiEG10355. fucU.

Phylogenomic databases

eggNOGiENOG4105NWF. Bacteria.
COG4154. LUCA.
HOGENOMiHOG000219041.
InParanoidiP0AEN8.
KOiK02431.
OMAiADLNFPA.
PhylomeDBiP0AEN8.

Enzyme and pathway databases

UniPathwayiUPA00956.
BioCyciEcoCyc:EG10355-MONOMER.
ECOL316407:JW2775-MONOMER.
MetaCyc:EG10355-MONOMER.
BRENDAi5.1.3.29. 2026.

Miscellaneous databases

EvolutionaryTraceiP0AEN8.
PROiP0AEN8.

Family and domain databases

Gene3Di3.40.1650.10. 1 hit.
HAMAPiMF_01662. L_fucose_rotase. 1 hit.
InterProiIPR023751. L-fucose_mutarotase.
IPR023750. RbsD-like.
IPR007721. RbsD_FucU.
[Graphical view]
PfamiPF05025. RbsD_FucU. 1 hit.
[Graphical view]
SUPFAMiSSF102546. SSF102546. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFUCM_ECOLI
AccessioniPrimary (citable) accession number: P0AEN8
Secondary accession number(s): P11555, Q2MA30, Q46923
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.