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Protein

L-cystine-binding protein FliY

Gene

fliY

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex FliY-YecC-YecS involved in L-cystine transport. The system can probably also transport L-cysteine, and it mediates accumulation of the toxic compounds L-selenaproline (SCA) and L-selenocystine (SeCys). Binds cystine (PubMed:8450713).2 Publications

Enzyme regulationi

The FliY-YecC-YecS system is inhibited by L-cystine, L-cysteine, DL-2,6-diaminopimelic acid and L-cystathionine, and is stimulated by D-cysteine.1 Publication

GO - Molecular functioni

  • amino acid binding Source: EcoCyc
  • ionotropic glutamate receptor activity Source: InterPro
  • L-cystine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • L-cystine transport Source: EcoCyc
  • sulfur utilization Source: EcoCyc

Keywordsi

Biological processAmino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7039-MONOMER
MetaCyc:G7039-MONOMER

Protein family/group databases

TCDBi3.A.1.3.10 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
L-cystine-binding protein FliYCurated
Short name:
CBP
Alternative name(s):
Protein FliY
Sulfate starvation-induced protein 7
Short name:
SSI7
Gene namesi
Name:fliY
Synonyms:yzzR
Ordered Locus Names:b1920, JW1905
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12680 fliY

Subcellular locationi

GO - Cellular componenti

  • membrane Source: InterPro
  • outer membrane-bounded periplasmic space Source: EcoCyc

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

The fliY-ydjN double mutant is completely resistant to both L-selenaproline and L-selenocystine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 293 PublicationsAdd BLAST29
ChainiPRO_000003175530 – 266L-cystine-binding protein FliYAdd BLAST237

Proteomic databases

PaxDbiP0AEM9
PRIDEiP0AEM9

2D gel databases

SWISS-2DPAGEiP0AEM9

Expressioni

Inductioni

Repressed by sulfate or cysteine.1 Publication

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (YecC), two transmembrane proteins (YecS) and a solute-binding protein (FliY).Curated

Protein-protein interaction databases

BioGridi4261251, 71 interactors
DIPiDIP-47980N
IntActiP0AEM9, 9 interactors
STRINGi316385.ECDH10B_2061

Structurei

3D structure databases

ProteinModelPortaliP0AEM9
SMRiP0AEM9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105EAB Bacteria
COG0834 LUCA
HOGENOMiHOG000031895
InParanoidiP0AEM9
KOiK02424
OMAiFDMVANQ
PhylomeDBiP0AEM9

Family and domain databases

InterProiView protein in InterPro
IPR001320 Iontro_rcpt
IPR018313 SBP_3_CS
IPR001638 Solute-binding_3/MltF_N
PfamiView protein in Pfam
PF00497 SBP_bac_3, 1 hit
SMARTiView protein in SMART
SM00062 PBPb, 1 hit
SM00079 PBPe, 1 hit
PROSITEiView protein in PROSITE
PS01039 SBP_BACTERIAL_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLAHLGRQA LMGVMAVALV AGMSVKSFAD EGLLNKVKER GTLLVGLEGT
60 70 80 90 100
YPPFSFQGDD GKLTGFEVEF AQQLAKHLGV EASLKPTKWD GMLASLDSKR
110 120 130 140 150
IDVVINQVTI SDERKKKYDF STPYTISGIQ ALVKKGNEGT IKTADDLKGK
160 170 180 190 200
KVGVGLGTNY EEWLRQNVQG VDVRTYDDDP TKYQDLRVGR IDAILVDRLA
210 220 230 240 250
ALDLVKKTND TLAVTGEAFS RQESGVALRK GNEDLLKAVN DAIAEMQKDG
260
TLQALSEKWF GADVTK
Length:266
Mass (Da):29,039
Last modified:December 20, 2005 - v1
Checksum:i1B088DF3F34E5D47
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18539 Genomic DNA Translation: AAC43545.1
U00096 Genomic DNA Translation: AAC74987.1
AP009048 Genomic DNA Translation: BAA15740.1
PIRiE64955
RefSeqiNP_416430.1, NC_000913.3
WP_001296168.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74987; AAC74987; b1920
BAA15740; BAA15740; BAA15740
GeneIDi948833
KEGGiecj:JW1905
eco:b1920
PATRICifig|511145.12.peg.2002

Similar proteinsi

Entry informationi

Entry nameiFLIY_ECOLI
AccessioniPrimary (citable) accession number: P0AEM9
Secondary accession number(s): P39174
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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