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Protein

RNA polymerase sigma factor FliA

Gene

fliA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor controls the expression of flagella-related genes.UniRule annotation4 Publications

Miscellaneous

The distinguishing feature of FliA promoters is a long -10 region (GCCGATAA). The upstream GC constitutes an extended -10 motif and is recognized by Arg-91 (PubMed:19400790).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi207 – 226H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Sigma factor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG11355-MONOMER
MetaCyc:EG11355-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma factor FliAUniRule annotation
Alternative name(s):
RNA polymerase sigma factor for flagellar operonUniRule annotation
Sigma FUniRule annotation
Sigma-27
Sigma-28UniRule annotation
Gene namesi
Name:fliAUniRule annotation
Synonyms:flaD, rpoF
Ordered Locus Names:b1922, JW1907
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11355 fliA

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutants have poor biofilms (decrease in biomass, surface coverage and mean thickness).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73Q → A: No change in activity. 1 Publication1
Mutagenesisi74R → A or W: Decrease in activity. 1 Publication1
Mutagenesisi78A → E: Decrease in activity. 1 Publication1
Mutagenesisi81D → A: Loss of activity. 1 Publication1
Mutagenesisi84R → A: Loss of activity. 1 Publication1
Mutagenesisi91R → A: Loss of activity. 1 Publication1
Mutagenesisi92S → A: No change in activity. 1 Publication1
Mutagenesisi94R → A: Decrease in activity. 1 Publication1
Mutagenesisi95R → A: No change in activity. 1 Publication1
Mutagenesisi96N → A: No change in activity. 1 Publication1
Mutagenesisi98R → A: Strong decrease in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000939811 – 239RNA polymerase sigma factor FliAAdd BLAST239

Proteomic databases

PaxDbiP0AEM6
PRIDEiP0AEM6

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
flgMP0AEM44EBI-549807,EBI-1121952

Protein-protein interaction databases

BioGridi4259656, 28 interactors
DIPiDIP-47959N
IntActiP0AEM6, 21 interactors
STRINGi316385.ECDH10B_2063

Structurei

3D structure databases

ProteinModelPortaliP0AEM6
SMRiP0AEM6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni16 – 88Sigma-70 factor domain-2Add BLAST73
Regioni96 – 166Sigma-70 factor domain-3Add BLAST71
Regioni185 – 233Sigma-70 factor domain-4Add BLAST49

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi43 – 46Interaction with polymerase core subunit RpoC4

Domaini

The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC (By similarity).By similarity
The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA (By similarity).By similarity

Sequence similaritiesi

Belongs to the sigma-70 factor family. FliA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DN4 Bacteria
COG1191 LUCA
HOGENOMiHOG000270271
InParanoidiP0AEM6
KOiK02405
OMAiIKFETYA
PhylomeDBiP0AEM6

Family and domain databases

HAMAPiMF_00962 Sigma70_FliA, 1 hit
InterProiView protein in InterPro
IPR014284 RNA_pol_sigma-70_dom
IPR000943 RNA_pol_sigma70
IPR007627 RNA_pol_sigma70_r2
IPR007624 RNA_pol_sigma70_r3
IPR007630 RNA_pol_sigma70_r4
IPR012845 RNA_pol_sigma_FliA_WhiG
IPR013325 RNA_pol_sigma_r2
IPR013324 RNA_pol_sigma_r3/r4-like
IPR028617 Sigma70_FliA
PfamiView protein in Pfam
PF04542 Sigma70_r2, 1 hit
PF04539 Sigma70_r3, 1 hit
PF04545 Sigma70_r4, 1 hit
PIRSFiPIRSF000770 RNA_pol_sigma-SigE/K, 1 hit
PRINTSiPR00046 SIGMA70FCT
SUPFAMiSSF88659 SSF88659, 2 hits
SSF88946 SSF88946, 1 hit
TIGRFAMsiTIGR02479 FliA_WhiG, 1 hit
TIGR02937 sigma70-ECF, 1 hit
PROSITEiView protein in PROSITE
PS00715 SIGMA70_1, 1 hit
PS00716 SIGMA70_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0AEM6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSLYTAEGV MDKHSLWQRY VPLVRHEALR LQVRLPASVE LDDLLQAGGI
60 70 80 90 100
GLLNAVERYD ALQGTAFTTY AVQRIRGAML DELRSRDWVP RSVRRNAREV
110 120 130 140 150
AQAIGQLEQE LGRNATETEV AERLGIDIAD YRQMLLDTNN SQLFSYDEWR
160 170 180 190 200
EEHGDSIELV TDDHQRENPL QQLLDSNLRQ RVMEAIETLP EREKLVLTLY
210 220 230
YQEELNLKEI GAVLEVGESR VSQLHSQAIK RLRTKLGKL
Length:239
Mass (Da):27,521
Last modified:December 20, 2005 - v1
Checksum:iB2930B44E1F24A33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36677 Genomic DNA Translation: AAC37011.1
U18539 Genomic DNA Translation: AAC43543.1
U00096 Genomic DNA Translation: AAC74989.1
AP009048 Genomic DNA Translation: BAA15742.1
PIRiJC4346
RefSeqiNP_416432.3, NC_000913.3
WP_001087467.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74989; AAC74989; b1922
BAA15742; BAA15742; BAA15742
GeneIDi948824
KEGGiecj:JW1907
eco:b1922
PATRICifig|1411691.4.peg.327

Similar proteinsi

Entry informationi

Entry nameiFLIA_ECOLI
AccessioniPrimary (citable) accession number: P0AEM6
Secondary accession number(s): P31804
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: June 20, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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