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Protein

Negative regulator of flagellin synthesis

Gene

flgM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the coupling of flagellin expression to flagellar assembly by preventing expression of the flagellin genes when a component of the middle class of proteins is defective. It negatively regulates flagellar genes by inhibiting the activity of FliA by directly binding to FliA.

GO - Molecular functioni

  • sigma factor antagonist activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Bacterial flagellum biogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G369-MONOMER.
ECOL316407:JW1058-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Negative regulator of flagellin synthesis
Alternative name(s):
Anti-sigma-28 factor
Gene namesi
Name:flgM
Ordered Locus Names:b1071, JW1058
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12664. flgM.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9797Negative regulator of flagellin synthesisPRO_0000087283Add
BLAST

Proteomic databases

EPDiP0AEM4.
PaxDbiP0AEM4.
PRIDEiP0AEM4.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
fliAP0AEM64EBI-1121952,EBI-549807

Protein-protein interaction databases

IntActiP0AEM4. 13 interactions.
STRINGi511145.b1071.

Structurei

3D structure databases

ProteinModelPortaliP0AEM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FlgM family.Curated

Phylogenomic databases

eggNOGiENOG4105M9M. Bacteria.
COG2747. LUCA.
HOGENOMiHOG000265658.
KOiK02398.
OMAiTQDIDMN.
OrthoDBiEOG68DD3J.

Family and domain databases

InterProiIPR007412. FlgM.
IPR031316. FlgM_C.
[Graphical view]
PfamiPF04316. FlgM. 1 hit.
[Graphical view]
SUPFAMiSSF101498. SSF101498. 1 hit.
TIGRFAMsiTIGR03824. FlgM_jcvi. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AEM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIDRTSPLK PVSTVQPRET TDAPVTNSRA AKTTASTSTS VTLSDAQAKL
60 70 80 90
MQPGSSDINL ERVEALKLAI RNGELKMDTG KIADALINEA QQDLQSN
Length:97
Mass (Da):10,341
Last modified:December 20, 2005 - v1
Checksum:i367D6F63324BF75D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19773 Genomic DNA. Translation: AAA61762.1.
U00096 Genomic DNA. Translation: AAC74155.1.
AP009048 Genomic DNA. Translation: BAA35879.1.
PIRiD64850.
RefSeqiNP_415589.1. NC_000913.3.
WP_000020880.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74155; AAC74155; b1071.
BAA35879; BAA35879; BAA35879.
GeneIDi946684.
KEGGiecj:JW1058.
eco:b1071.
PATRICi32117381. VBIEscCol129921_1113.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19773 Genomic DNA. Translation: AAA61762.1.
U00096 Genomic DNA. Translation: AAC74155.1.
AP009048 Genomic DNA. Translation: BAA35879.1.
PIRiD64850.
RefSeqiNP_415589.1. NC_000913.3.
WP_000020880.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AEM4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0AEM4. 13 interactions.
STRINGi511145.b1071.

Proteomic databases

EPDiP0AEM4.
PaxDbiP0AEM4.
PRIDEiP0AEM4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74155; AAC74155; b1071.
BAA35879; BAA35879; BAA35879.
GeneIDi946684.
KEGGiecj:JW1058.
eco:b1071.
PATRICi32117381. VBIEscCol129921_1113.

Organism-specific databases

EchoBASEiEB2530.
EcoGeneiEG12664. flgM.

Phylogenomic databases

eggNOGiENOG4105M9M. Bacteria.
COG2747. LUCA.
HOGENOMiHOG000265658.
KOiK02398.
OMAiTQDIDMN.
OrthoDBiEOG68DD3J.

Enzyme and pathway databases

BioCyciEcoCyc:G369-MONOMER.
ECOL316407:JW1058-MONOMER.

Miscellaneous databases

PROiP0AEM4.

Family and domain databases

InterProiIPR007412. FlgM.
IPR031316. FlgM_C.
[Graphical view]
PfamiPF04316. FlgM. 1 hit.
[Graphical view]
SUPFAMiSSF101498. SSF101498. 1 hit.
TIGRFAMsiTIGR03824. FlgM_jcvi. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of class II motility genes in Escherichia coli."
    Mytelka D.S., Chamberlin M.J.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The C-terminal half of the anti-sigma factor, FlgM, becomes structured when bound to its target, sigma 28."
    Daughdrill G.W., Chadsey M.S., Karlinsey J.E., Hughes K.T., Dahlquist F.W.
    Nat. Struct. Biol. 4:285-291(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiFLGM_ECOLI
AccessioniPrimary (citable) accession number: P0AEM4
Secondary accession number(s): P43532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 16, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.