P0AEM0 (FKBX_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
FKBP-type 16 kDa peptidyl-prolyl cis-trans isomerase
Short name=PPIase
EC=5.2.1.8

Alternative name(s):
Rotamase

Gene names

Name:	fkpB
Synonyms:	slpA, yaaD
Ordered Locus Names:	b0028, JW0026

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	PPIases accelerate the folding of proteins. Substrate specificity investigated with 'Suc-Ala-Xaa-Pro-Phe-4-nitroanilide' where Xaa is the amino acid tested, was found to be Phe > Leu >> Ile > Lys = Ala > Trp > His >> Gln.
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0).
Sequence similarities	Belongs to the FKBP-type PPIase family. Contains 1 PPIase FKBP-type domain.
Mass spectrometry	Molecular mass is 15946 Da from positions 2 - 149. Determined by ESI. Ref.5

Ontologies

Keywords
Molecular function	Isomerase Rotamase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from sequence or structural similarity. Source: RefGenome
Molecular function	FK506 binding Inferred from sequence or structural similarity. Source: RefGenome peptidyl-prolyl cis-trans isomerase activity Inferred from sequence or structural similarity. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 149	148	FKBP-type 16 kDa peptidyl-prolyl cis-trans isomerase		PRO_0000075369
Regions
	Domain	2 – 72	71	PPIase FKBP-type

Sequences

	Sequence		Length	Mass (Da)
	P0AEM0 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 485CB0472D571A6F	FASTA	149	16,081
	10 20 30 40 50 60 MSESVQSNSA VLVHFTLKLD DGTTAESTRN NGKPALFRLG DASLSEGLEQ HLLGLKVGDK 70 80 90 100 110 120 TTFSLEPDAA FGVPSPDLIQ YFSRREFMDA GEPEIGAIML FTAMDGSEMP GVIREINGDS 130 140 ITVDFNHPLA GQTVHFDIEV LEIDPALEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the lsp-dapB interval in Escherichia coli." Bouvier J., Stragier P. Nucleic Acids Res. 19:180-180(1991) [PubMed: 2011499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The Escherichia coli SlyD is a metal ion-regulated peptidyl-prolyl cis/trans-isomerase." Hottenrott S., Schumann T., Plueckthun A., Fischer G., Rahfeld J.-U. J. Biol. Chem. 272:15697-15701(1997) [PubMed: 9188461] [Abstract] Cited for: CHARACTERIZATION, MASS SPECTROMETRY. Strain: BL21.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54945 Genomic DNA. Translation: CAA38706.1. U00096 Genomic DNA. Translation: AAC73139.1. AP009048 Genomic DNA. Translation: BAB96597.1.
PIR	JE0402.
RefSeq	NP_414569.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AEM0.
SMR	P0AEM0. Positions 4-149.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-35792N.
IntAct	P0AEM0. 30 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000045; EBESCP00000000045; EBESCG00000000037. EBESCT00000000046; EBESCP00000000046; EBESCG00000000037. EBESCT00000000047; EBESCP00000000047; EBESCG00000000037. EBESCT00000000048; EBESCP00000000048; EBESCG00000000037. EBESCT00000016209; EBESCP00000015500; EBESCG00000015269.
GeneID	944807.
GenomeReviews	Gene locus JW0026 in contig AP009048_GR. Gene locus b0028 in contig U00096_GR.
KEGG	ecj:JW0026. eco:b0028.
PATRIC	32115147. VBIEscCol129921_0025.
Organism-specific databases
EchoBASE	EB1072.
EcoGene	EG11080. fkpB.
Phylogenomic databases
eggNOG	COG1047.
GeneTree	EBGT00050000009174.
HOGENOM	HBG731200.
OMA	TFKVGDG.
PhylomeDB	P0AEM0.
ProtClustDB	PRK15095.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11080-MONOMER. MetaCyc:EG11080-MONOMER.
Gene expression databases
Genevestigator	P0AEM0.
Family and domain databases
InterPro	IPR023566. PPIase_FKBP. IPR001179. PPIase_FKBP_dom. [Graphical view]
KO	K03774.
PANTHER	PTHR10516. PPIase_FKBP. 1 hit.
Pfam	PF00254. FKBP_C. 1 hit. [Graphical view]
PROSITE	PS50059. FKBP_PPIASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FKBX_ECOLI

Accession

Primary (citable) accession number: P0AEM0
Secondary accession number(s): P22563

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program