P0AEL6 (FEPB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferrienterobactin-binding periplasmic protein

Gene names

Name:	fepB
Ordered Locus Names:	b0592, JW0584

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds ferrienterobactin; part of the binding-protein-dependent transport system for uptake of ferrienterobactin.
Subcellular location	Periplasm.
Induction	Controlled in part by the amount of available iron.
Sequence similarities	Belongs to the bacterial solute-binding protein 8 family. Contains 1 Fe/B12 periplasmic-binding domain.

Ontologies

Keywords
Biological process	Ion transport Iron transport Transport
Cellular component	Periplasm
Domain	Signal
Ligand	Iron
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ion transport Inferred from electronic annotation. Source: UniProtKB-KW iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW response to DNA damage stimulus Inferred from expression pattern PubMed 11967071. Source: EcoliWiki
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

26

Potential

Chain

27 – 318

292

Ferrienterobactin-binding periplasmic protein

PRO_0000031821

Regions

Domain

48 – 318

271

Fe/B12 periplasmic-binding

Secondary structure

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318

Helix Strand Turn

Details...

Beta strand

30 – 35

6

Beta strand

38 – 42

5

Beta strand

47 – 52

6

Helix

54 – 62

9

Beta strand

67 – 72

6

Helix

87 – 89

3

Helix

90 – 95

6

Beta strand

99 – 105

7

Helix

108 – 112

5

Beta strand

117 – 125

9

Helix

130 – 132

3

Helix

133 – 137

5

Beta strand

142 – 145

4

Beta strand

148 – 150

3

Helix

152 – 163

12

Helix

166 – 186

21

Beta strand

191 – 200

10

Turn

201 – 204

4

Beta strand

205 – 209

5

Helix

214 – 221

8

Beta strand

243 – 248

6

Helix

252 – 256

5

Beta strand

259 – 264

6

Helix

269 – 277

9

Helix

279 – 281

3

Helix

285 – 288

4

Beta strand

292 – 294

3

Helix

297 – 299

3

Helix

304 – 318

15

Sequences

	Sequence		Length	Mass (Da)
	P0AEL6 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 807676D03A508079	FASTA	318	34,283
	10 20 30 40 50 60 MRLAPLYRNA LLLTGLLLSG IAAVQAADWP RQITDSRGTH TLESQPQRIV STSVTLTGSL 70 80 90 100 110 120 LAIDAPVIAS GATTPNNRVA DDQGFLRQWS KVAKERKLQR LYIGEPSAEA VAAQMPDLIL 130 140 150 160 170 180 ISATGGDSAL ALYDQLSTIA PTLIINYDDK SWQSLLTQLG EITGHEKQAA ERIAQFDKQL 190 200 210 220 230 240 AAAKEQIKLP PQPVTAIVYT AAAHSANLWT PESAQGQMLE QLGFTLAKLP AGLNASQSQG 250 260 270 280 290 300 KRHDIIQLGG ENLAAGLNGE SLFLFAGDQK DADAIYANPL LAHLPAVQNK QVYALGTETF 310 RLDYYSAMQV LDRLKALF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and regulation of the Escherichia coli gene for ferrienterobactin transport protein FepB." Elkins M.F., Earhart C.F. J. Bacteriol. 171:5443-5451(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Regulation of divergent transcription from the iron-responsive fepB-entC promoter-operator regions in Escherichia coli." Brickman T.J., Ozenberger B.A., McIntosh M.A. J. Mol. Biol. 212:669-682(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-57.
[6]	"Escherichia coli periplasmic protein FepB binds ferrienterobactin." Stephens D.L., Choe M.D., Earhart C.F. Microbiology 141:1647-1654(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M29730 Genomic DNA. Translation: AAA83853.1.
U82598 Genomic DNA. Translation: AAB40791.1.
U00096 Genomic DNA. Translation: AAC73693.1.
AP009048 Genomic DNA. Translation: BAE76347.1.
X53274 Genomic DNA. Translation: CAA37370.1.

PIR

JV0045.

RefSeq

NP_415124.1. NC_000913.2.
YP_488881.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3TLK	X-ray	1.85	A/B/C	1-318	[»]

ProteinModelPortal

P0AEL6.

SMR

P0AEL6. Positions 29-318.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0AEL6. 14 interactions.

STRING

511145.b0592.

Proteomic databases

PaxDb

P0AEL6.

PRIDE

P0AEL6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73693; AAC73693; b0592.
BAE76347; BAE76347; BAE76347.

GeneID

12931986.
947538.

KEGG

ecj:Y75_p0581.
eco:b0592.

PATRIC

32116360. VBIEscCol129921_0620.

Organism-specific databases

EchoBASE

EB0290.

EcoGene

EG10294. fepB.

Phylogenomic databases

eggNOG

COG4592.

HOGENOM

HOG000117867.

KO

K02016.

OMA

KSWQELA.

ProtClustDB

PRK10957.

Enzyme and pathway databases

BioCyc

EcoCyc:FEPB-MONOMER.
ECOL316407:JW0584-MONOMER.

Gene expression databases

Genevestigator

P0AEL6.

Family and domain databases

InterPro

IPR002491. ABC_transptr_periplasmic_BD.
[Graphical view]

Pfam

PF01497. Peripla_BP_2. 1 hit.
[Graphical view]

PROSITE

PS50983. FE_B12_PBP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FEPB_ECOLI

Accession

Primary (citable) accession number: P0AEL6
Secondary accession number(s): P14609, Q2MBK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program