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P0AEK6

- FABI_SHIFL

UniProt

P0AEK6 - FABI_SHIFL

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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene
fabI, SF1293, S1375
Organism
Shigella flexneri
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis By similarity.

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131NAD; via carbonyl oxygen By similarity
Binding sitei40 – 401NAD By similarity
Binding sitei92 – 921NAD; via carbonyl oxygen By similarity
Binding sitei95 – 951Substrate; via amide nitrogen and carbonyl oxygen By similarity
Active sitei146 – 1461Proton acceptor By similarity
Active sitei156 – 1561Proton acceptor By similarity
Binding sitei163 – 1631NAD By similarity
Sitei201 – 2011Involved in acyl-ACP binding By similarity
Sitei204 – 2041Involved in acyl-ACP binding By similarity
Sitei205 – 2051Involved in acyl-ACP binding By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 202NAD By similarity
Nucleotide bindingi64 – 652NAD By similarity
Nucleotide bindingi192 – 1965NAD By similarity

GO - Molecular functioni

  1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB
  2. fatty acid elongation Source: UniProtKB
  3. protein homotetramerization Source: UniProtKB
  4. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic resistance, Biotin biosynthesis, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00078.
UPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:SF1293, S1375
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 262261Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000054911Add
BLAST

Proteomic databases

PaxDbiP0AEK6.
PRIDEiP0AEK6.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi198214.SF1293.

Structurei

3D structure databases

ProteinModelPortaliP0AEK6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiKLSIAWA.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEK6-1 [UniParc]FASTAAdd to Basket

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MGFLSGKRIL VTGVASKLSI AYGIAQAMHR EGAELAFTYQ NDKLKGRVEE    50
FAAQLGSDIV LQCDVAEDAS IDTMFAELGK VWPKFDGFVH SIGFAPGDQL 100
DGDYVNAVTR EGFKIAHDIS SYSFVAMAKA CRSMLNPGSA LLTLSYLGAE 150
RAIPNYNVMG LAKASLEANV RYMANAMGPE GVRVNAISAG PIRTLAASGI 200
KDFRKMLAHC EAVTPIRRTV TIEDVGNSAA FLCSDLSAGI SGEVVHVDGG 250
FSIAAMNELE LK 262
Length:262
Mass (Da):27,864
Last modified:January 23, 2007 - v2
Checksum:i436A89AF349D1866
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005674 Genomic DNA. Translation: AAN42904.1.
AE014073 Genomic DNA. Translation: AAP16787.1.
RefSeqiNP_707197.1. NC_004337.2.
NP_836980.1. NC_004741.1.

Genome annotation databases

EnsemblBacteriaiAAN42904; AAN42904; SF1293.
AAP16787; AAP16787; S1375.
GeneIDi1024254.
1077753.
KEGGisfl:SF1293.
sfx:S1375.
PATRICi18704006. VBIShiFle31049_1507.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005674 Genomic DNA. Translation: AAN42904.1 .
AE014073 Genomic DNA. Translation: AAP16787.1 .
RefSeqi NP_707197.1. NC_004337.2.
NP_836980.1. NC_004741.1.

3D structure databases

ProteinModelPortali P0AEK6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198214.SF1293.

Chemistry

BindingDBi P0AEK6.

Proteomic databases

PaxDbi P0AEK6.
PRIDEi P0AEK6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN42904 ; AAN42904 ; SF1293 .
AAP16787 ; AAP16787 ; S1375 .
GeneIDi 1024254.
1077753.
KEGGi sfl:SF1293.
sfx:S1375.
PATRICi 18704006. VBIShiFle31049_1507.

Phylogenomic databases

eggNOGi COG0623.
KOi K00208.
OMAi KLSIAWA.
OrthoDBi EOG6HF644.

Enzyme and pathway databases

UniPathwayi UPA00078 .
UPA00094 .

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSi PR00081. GDHRDH.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiFABI_SHIFL
AccessioniPrimary (citable) accession number: P0AEK6
Secondary accession number(s): P29132
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The antibiotic diazaborine interferes with the activity by binding to the protein By similarity.

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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