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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis (By similarity).By similarity

Miscellaneous

The antibiotic diazaborine interferes with the activity by binding to the protein.By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13NAD; via carbonyl oxygenBy similarity1
Binding sitei40NADBy similarity1
Binding sitei92NAD; via carbonyl oxygenBy similarity1
Binding sitei95Substrate; via amide nitrogen and carbonyl oxygenBy similarity1
Active sitei146Proton acceptorBy similarity1
Active sitei156Proton acceptorBy similarity1
Binding sitei163NADBy similarity1
Sitei201Involved in acyl-ACP bindingBy similarity1
Sitei204Involved in acyl-ACP bindingBy similarity1
Sitei205Involved in acyl-ACP bindingBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 20NADBy similarity2
Nucleotide bindingi64 – 65NADBy similarity2
Nucleotide bindingi192 – 196NADBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAntibiotic resistance, Biotin biosynthesis, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00078
UPA00094

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:Z2512, ECs1861
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000549002 – 262Enoyl-[acyl-carrier-protein] reductase [NADH] FabIAdd BLAST261

Proteomic databases

PRIDEiP0AEK5

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi155864.Z2512

Structurei

3D structure databases

ProteinModelPortaliP0AEK5
SMRiP0AEK5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CSJ Bacteria
COG0623 LUCA
KOiK00208
OMAiGILDMIH

Family and domain databases

InterProiView protein in InterPro
IPR014358 Enoyl-ACP_Rdtase_NADH
IPR036291 NAD(P)-bd_dom_sf
IPR002347 SDR_fam
PANTHERiPTHR43159:SF2 PTHR43159:SF2, 1 hit
PIRSFiPIRSF000094 Enoyl-ACP_rdct, 1 hit
PRINTSiPR00081 GDHRDH
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFLSGKRIL VTGVASKLSI AYGIAQAMHR EGAELAFTYQ NDKLKGRVEE
60 70 80 90 100
FAAQLGSDIV LQCDVAEDAS IDTMFAELGK VWPKFDGFVH SIGFAPGDQL
110 120 130 140 150
DGDYVNAVTR EGFKIAHDIS SYSFVAMAKA CRSMLNPGSA LLTLSYLGAE
160 170 180 190 200
RAIPNYNVMG LAKASLEANV RYMANAMGPE GVRVNAISAG PIRTLAASGI
210 220 230 240 250
KDFRKMLAHC EAVTPIRRTV TIEDVGNSAA FLCSDLSAGI SGEVVHVDGG
260
FSIAAMNELE LK
Length:262
Mass (Da):27,864
Last modified:January 23, 2007 - v2
Checksum:i436A89AF349D1866
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG56524.1
BA000007 Genomic DNA Translation: BAB35284.1
PIRiE90861
H85757
RefSeqiNP_309888.1, NC_002695.1
WP_000506490.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG56524; AAG56524; Z2512
BAB35284; BAB35284; BAB35284
GeneIDi912787
KEGGiece:Z2512
ecs:ECs1861
PATRICifig|386585.9.peg.1962

Similar proteinsi

Entry informationi

Entry nameiFABI_ECO57
AccessioniPrimary (citable) accession number: P0AEK5
Secondary accession number(s): P29132
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health