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P0AEK5

- FABI_ECO57

UniProt

P0AEK5 - FABI_ECO57

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Protein
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
Gene
fabI, Z2512, ECs1861
Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis By similarity.

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131NAD; via carbonyl oxygen By similarity
Binding sitei40 – 401NAD By similarity
Binding sitei92 – 921NAD; via carbonyl oxygen By similarity
Binding sitei95 – 951Substrate; via amide nitrogen and carbonyl oxygen By similarity
Active sitei146 – 1461Proton acceptor By similarity
Active sitei156 – 1561Proton acceptor By similarity
Binding sitei163 – 1631NAD By similarity
Sitei201 – 2011Involved in acyl-ACP binding By similarity
Sitei204 – 2041Involved in acyl-ACP binding By similarity
Sitei205 – 2051Involved in acyl-ACP binding By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 202NAD By similarity
Nucleotide bindingi64 – 652NAD By similarity
Nucleotide bindingi192 – 1965NAD By similarity

GO - Molecular functioni

  1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB
  2. fatty acid elongation Source: UniProtKB
  3. protein homotetramerization Source: UniProtKB
  4. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic resistance, Biotin biosynthesis, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciECOL386585:GJFA-1837-MONOMER.
ECOO157:FABI-MONOMER.
UniPathwayiUPA00078.
UPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:Z2512, ECs1861
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 262261Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
PRO_0000054900Add
BLAST

Proteomic databases

PRIDEiP0AEK5.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

MINTiMINT-1251192.
STRINGi155864.Z2512.

Structurei

3D structure databases

ProteinModelPortaliP0AEK5.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiKLSIAWA.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEK5-1 [UniParc]FASTAAdd to Basket

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MGFLSGKRIL VTGVASKLSI AYGIAQAMHR EGAELAFTYQ NDKLKGRVEE    50
FAAQLGSDIV LQCDVAEDAS IDTMFAELGK VWPKFDGFVH SIGFAPGDQL 100
DGDYVNAVTR EGFKIAHDIS SYSFVAMAKA CRSMLNPGSA LLTLSYLGAE 150
RAIPNYNVMG LAKASLEANV RYMANAMGPE GVRVNAISAG PIRTLAASGI 200
KDFRKMLAHC EAVTPIRRTV TIEDVGNSAA FLCSDLSAGI SGEVVHVDGG 250
FSIAAMNELE LK 262
Length:262
Mass (Da):27,864
Last modified:January 23, 2007 - v2
Checksum:i436A89AF349D1866
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG56524.1.
BA000007 Genomic DNA. Translation: BAB35284.1.
PIRiE90861.
H85757.
RefSeqiNP_287908.1. NC_002655.2.
NP_309888.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG56524; AAG56524; Z2512.
BAB35284; BAB35284; BAB35284.
GeneIDi912787.
961367.
KEGGiece:Z2512.
ecs:ECs1861.
PATRICi18353671. VBIEscCol44059_2052.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG56524.1 .
BA000007 Genomic DNA. Translation: BAB35284.1 .
PIRi E90861.
H85757.
RefSeqi NP_287908.1. NC_002655.2.
NP_309888.1. NC_002695.1.

3D structure databases

ProteinModelPortali P0AEK5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1251192.
STRINGi 155864.Z2512.

Chemistry

BindingDBi P0AEK5.

Proteomic databases

PRIDEi P0AEK5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG56524 ; AAG56524 ; Z2512 .
BAB35284 ; BAB35284 ; BAB35284 .
GeneIDi 912787.
961367.
KEGGi ece:Z2512.
ecs:ECs1861.
PATRICi 18353671. VBIEscCol44059_2052.

Phylogenomic databases

eggNOGi COG0623.
KOi K00208.
OMAi KLSIAWA.
OrthoDBi EOG6HF644.

Enzyme and pathway databases

UniPathwayi UPA00078 .
UPA00094 .
BioCyci ECOL386585:GJFA-1837-MONOMER.
ECOO157:FABI-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSi PR00081. GDHRDH.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiFABI_ECO57
AccessioniPrimary (citable) accession number: P0AEK5
Secondary accession number(s): P29132
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The antibiotic diazaborine interferes with the activity by binding to the protein By similarity.

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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