Reviewed, UniProtKB/Swiss-Prot P0AEK5 (FABI_ECO57)

Names and originHide

Protein names

Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH]
EC=1.3.1.9

Alternative name(s):
NADH-dependent enoyl-ACP reductase

Gene names

Name:	fabI
Ordered Locus Names:	Z2512, ECs1861

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributesHide

Sequence length	262 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)Hide

Catalytic activity	Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Miscellaneous	The antibiotic diazaborine interferes with the activity by binding to the protein By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

OntologiesHide

Keywords
Biological process	Antibiotic resistance Fatty acid biosynthesis Lipid synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 262	261	Enoyl-[acyl-carrier-protein] reductase [NADH]		PRO_0000054900
Regions
	Nucleotide binding	10 – 36	27	NAD By similarity
Sites
	Active site	156	1	Proton acceptor By similarity

SequencesHide

	Sequence		Length	Mass (Da)
	P0AEK5-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 436A89AF349D1866	FASTA	262	27,864
	10 20 30 40 50 60 MGFLSGKRIL VTGVASKLSI AYGIAQAMHR EGAELAFTYQ NDKLKGRVEE FAAQLGSDIV 70 80 90 100 110 120 LQCDVAEDAS IDTMFAELGK VWPKFDGFVH SIGFAPGDQL DGDYVNAVTR EGFKIAHDIS 130 140 150 160 170 180 SYSFVAMAKA CRSMLNPGSA LLTLSYLGAE RAIPNYNVMG LAKASLEANV RYMANAMGPE 190 200 210 220 230 240 GVRVNAISAG PIRTLAASGI KDFRKMLAHC EAVTPIRRTV TIEDVGNSAA FLCSDLSAGI 250 260 SGEVVHVDGG FSIAAMNELE LK

ReferencesHide

[1]	"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]	"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
+	Additional computationally mapped references.

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG56524.1. BA000007 Genomic DNA. Translation: BAB35284.1.
PIR	E90861. H85757.
RefSeq	NP_287908.1. NP_309888.1.
3D structure databases
SMR	P0AEK5. Positions 2-260.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1251192.
Genome annotation databases
EnsemblBacteria	EBESCT00000025202; EBESCP00000024095; EBESCG00000024256. EBESCT00000056775; EBESCP00000054603; EBESCG00000055823.
GeneID	912787. 961367.
GenomeReviews	Gene locus Z2512 in contig AE005174_GR. Gene locus ECs1861 in contig BA000007_GR.
KEGG	ece:Z2512. ecs:ECs1861.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750976.
OMA	DCDVGSD.
ProtClustDB	PRK07984.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS1861-MONOMER.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR014358. Enoyl-ACP_Rdtase_NADH. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit. PTHR19410:SF12. Enoyl-ACP_rdct. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
ProtoNet	Search...
Other Resources
BindingDB	P0AEK5.

Entry informationHide

Entry name

FABI_ECO57

Accession

Primary (citable) accession number: P0AEK5
Secondary accession number(s): P29132

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	January 23, 2007
Last modified:	July 13, 2010
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)