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P0AEK3

- FABG_SHIFL

UniProt

P0AEK3 - FABG_SHIFL

Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

    Catalytic activityi

    (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei37 – 371NADPBy similarity
    Metal bindingi50 – 501Calcium 1; via carbonyl oxygen; shared with dimeric partnerBy similarity
    Metal bindingi53 – 531Calcium 1; via carbonyl oxygen; shared with dimeric partnerBy similarity
    Binding sitei86 – 861NADP; via carbonyl oxygenBy similarity
    Binding sitei138 – 1381SubstrateBy similarity
    Metal bindingi145 – 1451Calcium 2By similarity
    Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation
    Binding sitei184 – 1841NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Metal bindingi233 – 2331Calcium 3; shared with dimeric partnerBy similarity
    Metal bindingi234 – 2341Calcium 3; via carbonyl oxygen; shared with dimeric partnerBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 154NADPBy similarity
    Nucleotide bindingi59 – 602NADPBy similarity
    Nucleotide bindingi151 – 1555NADPBy similarity

    GO - Molecular functioni

    1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW
    3. NAD binding Source: InterPro
    4. NADP binding Source: UniProtKB

    GO - Biological processi

    1. fatty acid elongation Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
    Alternative name(s):
    3-ketoacyl-acyl carrier protein reductase
    Beta-Ketoacyl-acyl carrier protein reductase
    Beta-ketoacyl-ACP reductase
    Gene namesi
    Name:fabG
    Ordered Locus Names:SF1097, S1177
    OrganismiShigella flexneri
    Taxonomic identifieri623 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
    ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2442443-oxoacyl-[acyl-carrier-protein] reductase FabGPRO_0000054682Add
    BLAST

    Proteomic databases

    PaxDbiP0AEK3.
    PRIDEiP0AEK3.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi198214.SF1097.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AEK3.
    SMRiP0AEK3. Positions 2-244.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    KOiK00059.
    OMAiDEFGAID.
    OrthoDBiEOG6N3CR8.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR011284. 3oxo_ACP_reduc.
    IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AEK3-1 [UniParc]FASTAAdd to Basket

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    MNFEGKIALV TGASRGIGRA IAETLAARGA KVIGTATSEN GAQAISDYLG    50
    ANGKGLMLNV TDPASIESVL EKIRAEFGEV DILVNNAGIT RDNLLMRMKD 100
    EEWNDIIETN LSSVFRLSKA VMRAMMKKRH GRIITIGSVV GTMGNGGQAN 150
    YAAAKAGLIG FSKSLAREVA SRGITVNVVA PGFIETDMTR ALSDDQRAGI 200
    LAQVPAGRLG GAQEIANAVA FLASDEAAYI TGETLHVNGG MYMV 244
    Length:244
    Mass (Da):25,560
    Last modified:December 20, 2005 - v1
    Checksum:i48EC1F2A7F7EEFD9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005674 Genomic DNA. Translation: AAN42716.1.
    AE014073 Genomic DNA. Translation: AAP16604.1.
    RefSeqiNP_707009.1. NC_004337.2.
    NP_836798.1. NC_004741.1.

    Genome annotation databases

    EnsemblBacteriaiAAN42716; AAN42716; SF1097.
    AAP16604; AAP16604; S1177.
    GeneIDi1026267.
    1077578.
    KEGGisfl:SF1097.
    sfx:S1177.
    PATRICi18703556. VBIShiFle31049_1283.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005674 Genomic DNA. Translation: AAN42716.1 .
    AE014073 Genomic DNA. Translation: AAP16604.1 .
    RefSeqi NP_707009.1. NC_004337.2.
    NP_836798.1. NC_004741.1.

    3D structure databases

    ProteinModelPortali P0AEK3.
    SMRi P0AEK3. Positions 2-244.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198214.SF1097.

    Proteomic databases

    PaxDbi P0AEK3.
    PRIDEi P0AEK3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN42716 ; AAN42716 ; SF1097 .
    AAP16604 ; AAP16604 ; S1177 .
    GeneIDi 1026267.
    1077578.
    KEGGi sfl:SF1097.
    sfx:S1177.
    PATRICi 18703556. VBIShiFle31049_1283.

    Phylogenomic databases

    eggNOGi COG1028.
    KOi K00059.
    OMAi DEFGAID.
    OrthoDBi EOG6N3CR8.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR011284. 3oxo_ACP_reduc.
    IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    TIGRFAMsi TIGR01830. 3oxo_ACP_reduc. 1 hit.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
      Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
      , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
      Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 301 / Serotype 2a.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700930 / 2457T / Serotype 2a.

    Entry informationi

    Entry nameiFABG_SHIFL
    AccessioniPrimary (citable) accession number: P0AEK3
    Secondary accession number(s): P25716, P78221, Q47202
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Calcium ions stabilize the structure, and may inhibit FabG activity by obstructing access to the active site.By similarity

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3