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P0AEK3

- FABG_SHIFL

UniProt

P0AEK3 - FABG_SHIFL

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Protein
3-oxoacyl-[acyl-carrier-protein] reductase FabG
Gene
fabG, SF1097, S1177
Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis By similarity.

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371NADP By similarity
Metal bindingi50 – 501Calcium 1; via carbonyl oxygen; shared with dimeric partner By similarity
Metal bindingi53 – 531Calcium 1; via carbonyl oxygen; shared with dimeric partner By similarity
Binding sitei86 – 861NADP; via carbonyl oxygen By similarity
Binding sitei138 – 1381Substrate By similarity
Metal bindingi145 – 1451Calcium 2 By similarity
Active sitei151 – 1511Proton acceptor By similarity
Binding sitei184 – 1841NADP; via amide nitrogen and carbonyl oxygen By similarity
Metal bindingi233 – 2331Calcium 3; shared with dimeric partner By similarity
Metal bindingi234 – 2341Calcium 3; via carbonyl oxygen; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 154NADP By similarity
Nucleotide bindingi59 – 602NADP By similarity
Nucleotide bindingi151 – 1555NADP By similarity

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB
  2. NAD binding Source: InterPro
  3. NADP binding Source: UniProtKB
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fatty acid elongation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase
Gene namesi
Name:fabG
Ordered Locus Names:SF1097, S1177
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2442443-oxoacyl-[acyl-carrier-protein] reductase FabG
PRO_0000054682Add
BLAST

Proteomic databases

PaxDbiP0AEK3.
PRIDEiP0AEK3.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi198214.SF1097.

Structurei

3D structure databases

ProteinModelPortaliP0AEK3.
SMRiP0AEK3. Positions 2-244.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
KOiK00059.
OMAiDEFGAID.
OrthoDBiEOG6N3CR8.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEK3-1 [UniParc]FASTAAdd to Basket

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MNFEGKIALV TGASRGIGRA IAETLAARGA KVIGTATSEN GAQAISDYLG    50
ANGKGLMLNV TDPASIESVL EKIRAEFGEV DILVNNAGIT RDNLLMRMKD 100
EEWNDIIETN LSSVFRLSKA VMRAMMKKRH GRIITIGSVV GTMGNGGQAN 150
YAAAKAGLIG FSKSLAREVA SRGITVNVVA PGFIETDMTR ALSDDQRAGI 200
LAQVPAGRLG GAQEIANAVA FLASDEAAYI TGETLHVNGG MYMV 244
Length:244
Mass (Da):25,560
Last modified:December 20, 2005 - v1
Checksum:i48EC1F2A7F7EEFD9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005674 Genomic DNA. Translation: AAN42716.1.
AE014073 Genomic DNA. Translation: AAP16604.1.
RefSeqiNP_707009.1. NC_004337.2.
NP_836798.1. NC_004741.1.

Genome annotation databases

EnsemblBacteriaiAAN42716; AAN42716; SF1097.
AAP16604; AAP16604; S1177.
GeneIDi1026267.
1077578.
KEGGisfl:SF1097.
sfx:S1177.
PATRICi18703556. VBIShiFle31049_1283.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005674 Genomic DNA. Translation: AAN42716.1 .
AE014073 Genomic DNA. Translation: AAP16604.1 .
RefSeqi NP_707009.1. NC_004337.2.
NP_836798.1. NC_004741.1.

3D structure databases

ProteinModelPortali P0AEK3.
SMRi P0AEK3. Positions 2-244.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198214.SF1097.

Proteomic databases

PaxDbi P0AEK3.
PRIDEi P0AEK3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN42716 ; AAN42716 ; SF1097 .
AAP16604 ; AAP16604 ; S1177 .
GeneIDi 1026267.
1077578.
KEGGi sfl:SF1097.
sfx:S1177.
PATRICi 18703556. VBIShiFle31049_1283.

Phylogenomic databases

eggNOGi COG1028.
KOi K00059.
OMAi DEFGAID.
OrthoDBi EOG6N3CR8.

Enzyme and pathway databases

UniPathwayi UPA00094 .

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsi TIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiFABG_SHIFL
AccessioniPrimary (citable) accession number: P0AEK3
Secondary accession number(s): P25716, P78221, Q47202
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Calcium ions stabilize the structure, and may inhibit FabG activity by obstructing access to the active site By similarity.

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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