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Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

Miscellaneous

Calcium ions stabilize the structure, and may inhibit FabG activity by obstructing access to the active site.By similarity

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37NADPBy similarity1
Metal bindingi50Calcium 1; via carbonyl oxygen; shared with dimeric partnerBy similarity1
Metal bindingi53Calcium 1; via carbonyl oxygen; shared with dimeric partnerBy similarity1
Binding sitei86NADP; via carbonyl oxygenBy similarity1
Binding sitei138SubstrateBy similarity1
Metal bindingi145Calcium 2By similarity1
Active sitei151Proton acceptorPROSITE-ProRule annotation1
Binding sitei184NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Metal bindingi233Calcium 3; shared with dimeric partnerBy similarity1
Metal bindingi234Calcium 3; via carbonyl oxygen; shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 15NADPBy similarity4
Nucleotide bindingi59 – 60NADPBy similarity2
Nucleotide bindingi151 – 155NADPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandCalcium, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase
Gene namesi
Name:fabG
Ordered Locus Names:SF1097, S1177
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000546821 – 2443-oxoacyl-[acyl-carrier-protein] reductase FabGAdd BLAST244

Proteomic databases

PaxDbiP0AEK3.
PRIDEiP0AEK3.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0AEK3.
SMRiP0AEK3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CHR. Bacteria.
ENOG410XNW1. LUCA.
KOiK00059.
OMAiGQMGNPG.

Family and domain databases

InterProiView protein in InterPro
IPR011284. 3oxo_ACP_reduc.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiView protein in PROSITE
PS00061. ADH_SHORT. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AEK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFEGKIALV TGASRGIGRA IAETLAARGA KVIGTATSEN GAQAISDYLG
60 70 80 90 100
ANGKGLMLNV TDPASIESVL EKIRAEFGEV DILVNNAGIT RDNLLMRMKD
110 120 130 140 150
EEWNDIIETN LSSVFRLSKA VMRAMMKKRH GRIITIGSVV GTMGNGGQAN
160 170 180 190 200
YAAAKAGLIG FSKSLAREVA SRGITVNVVA PGFIETDMTR ALSDDQRAGI
210 220 230 240
LAQVPAGRLG GAQEIANAVA FLASDEAAYI TGETLHVNGG MYMV
Length:244
Mass (Da):25,560
Last modified:December 20, 2005 - v1
Checksum:i48EC1F2A7F7EEFD9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN42716.1.
AE014073 Genomic DNA. Translation: AAP16604.1.
RefSeqiNP_707009.1. NC_004337.2.
WP_001008535.1. NZ_MSKG02000174.1.

Genome annotation databases

EnsemblBacteriaiAAN42716; AAN42716; SF1097.
AAP16604; AAP16604; S1177.
GeneIDi1026267.
KEGGisfl:SF1097.
sfx:S1177.
PATRICifig|198214.7.peg.1285.

Similar proteinsi

Entry informationi

Entry nameiFABG_SHIFL
AccessioniPrimary (citable) accession number: P0AEK3
Secondary accession number(s): P25716, P78221, Q47202
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 27, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families