Reviewed, UniProtKB/Swiss-Prot P0AEJ6 (EUTB_ECOLI)

Names and origin

Protein names

Recommended name:
    Ethanolamine ammonia-lyase heavy chain
    EC=4.3.1.7
Alternative name(s):
    Ethanolamine ammonia-lyase large subunit

Gene names

Name:	eutB
Ordered Locus Names:	b2441, JW2434

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Ethanolamine = acetaldehyde + NH3.
Cofactor	Adenosylcobalamin.
Pathway	Amine and polyamine degradation; ethanolamine degradation.
Subunit structure	Heterodimer of two non-identical chains.

Ontologies

Keywords
Ligand	Cobalt
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW ethanolamine ammonia-lyase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	1	EBI-1128853,EBI-543750

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	Ethanolamine ammonia-lyase heavy chain		PRO_0000087081

Sequences

	Sequence		Length	Mass (Da)
	P0AEJ6-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 21C14FC469716060	FASTA	453	49,403
	10 20 30 40 50 60 MKLKTTLFGN VYQFKDVKEV LAKANELRSG DVLAGVAAAS SQERVAAKQV LSEMTVADIR 70 80 90 100 110 120 NNPVIAYEDD CVTRLIQDDV NETAYNQIKN WSISELREYV LSDETSVDDI AFTRKGLTSE 130 140 150 160 170 180 VVAAVAKICS NADLIYGAKK MPVIKKANTT IGIPGTFSAR LQPNDTRDDV QSIAAQIYEG 190 200 210 220 230 240 LSFGVGDAVI GVNPVTDDVE NLSRVLDTIY GVIDKFNIPT QGCVLAHVTT QIEAIRRGAP 250 260 270 280 290 300 GGLIFQSICG SEKGLKEFGV ELAMLDEARA VGAEFNRIAG ENCLYFETGQ GSALSAGANF 310 320 330 340 350 360 GADQVTMEAR NYGLARHYDP FIVNTVVGFI GPEYLYNDRQ IIRAGLEDHF MGKLSGISMG 370 380 390 400 410 420 CDCCYTNHAD ADQNLNENLM ILLATAGCNY IMGMPLGDDI MLNYQTTAFH DTATVRQLLN 430 440 450 LRPSPEFERW LESMGIMANG RLTKRAGDPS LFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Cloning, sequencing, and expression of the genes encoding the adenosylcobalamin-dependent ethanolamine ammonia-lyase of Salmonella typhimurium." Faust L.R.P., Connor J.A., Roof D.M., Hoch J.A., Babior B.M. J. Biol. Chem. 265:12462-12466(1990) [PubMed: 2197274] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-30.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00096 Genomic DNA. Translation: AAC75494.1. Different initiation. AP009048 Genomic DNA. Translation: BAA16323.1.
PIR	H65018.
RefSeq	AP_003035.1. NP_416936.4.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AEJ6. 1 interaction.
Genome annotation databases
GeneID	946924.
GenomeReviews	Gene locus JW2434 in contig AP009048_GR. Gene locus b2441 in contig U00096_GR.
KEGG	ecj:JW2434. eco:b2441.
Organism-specific databases
EchoBASE	EB4299.
EcoGene	EG50006. eutB.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AEJ6.
OMA	P0AEJ6. EARCYGL.
Enzyme and pathway databases
BioCyc	EcoCyc:EUTB-MON. MetaCyc:EUTB-MON.
Family and domain databases
InterPro	IPR010628. EutB. [Graphical view]
Pfam	PF06751. EutB. 1 hit. [Graphical view]
PIRSF	PIRSF018788. EutB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

EUTB_ECOLI

Accession

Primary (citable) accession number: P0AEJ6
Secondary accession number(s): P19635, P78094, P78300

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)