P0AEJ6 (EUTB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ethanolamine ammonia-lyase heavy chain
EC=4.3.1.7

Alternative name(s):
Ethanolamine ammonia-lyase large subunit

Gene names

Name:	eutB
Ordered Locus Names:	b2441, JW2434

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Ethanolamine = acetaldehyde + NH3.
Cofactor	Adenosylcobalamin.
Pathway	Amine and polyamine degradation; ethanolamine degradation.
Subunit structure	Heterodimer of two non-identical chains.

Ontologies

Keywords
Ligand	Cobalt
Molecular function	Lyase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	ethanolamine ammonia-lyase complex Inferred from direct assay. Source: EcoCyc
Molecular function	ethanolamine ammonia-lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 453

453

Ethanolamine ammonia-lyase heavy chain

PRO_0000087081

Secondary structure

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453

Helix Strand Turn

Details...

Beta strand

4 – 6

3

Beta strand

11 – 13

3

Helix

17 – 23

7

Helix

41 – 53

13

Helix

56 – 60

5

Helix

67 – 69

3

Helix

71 – 78

8

Helix

82 – 88

7

Helix

93 – 101

9

Helix

107 – 113

7

Helix

119 – 127

9

Helix

131 – 135

5

Beta strand

145 – 151

7

Beta strand

158 – 160

3

Helix

170 – 178

9

Helix

199 – 209

11

Turn

213 – 217

5

Helix

228 – 236

9

Beta strand

244 – 247

4

Helix

262 – 270

9

Beta strand

284 – 288

5

Turn

293 – 295

3

Helix

304 – 311

8

Helix

315 – 318

4

Beta strand

321 – 326

6

Helix

338 – 343

6

Helix

347 – 350

4

Helix

373 – 385

13

Turn

400 – 403

4

Helix

409 – 419

11

Helix

425 – 433

9

Helix

449 – 452

4

Sequences

	Sequence		Length	Mass (Da)
	P0AEJ6 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 21C14FC469716060	FASTA	453	49,403
	10 20 30 40 50 60 MKLKTTLFGN VYQFKDVKEV LAKANELRSG DVLAGVAAAS SQERVAAKQV LSEMTVADIR 70 80 90 100 110 120 NNPVIAYEDD CVTRLIQDDV NETAYNQIKN WSISELREYV LSDETSVDDI AFTRKGLTSE 130 140 150 160 170 180 VVAAVAKICS NADLIYGAKK MPVIKKANTT IGIPGTFSAR LQPNDTRDDV QSIAAQIYEG 190 200 210 220 230 240 LSFGVGDAVI GVNPVTDDVE NLSRVLDTIY GVIDKFNIPT QGCVLAHVTT QIEAIRRGAP 250 260 270 280 290 300 GGLIFQSICG SEKGLKEFGV ELAMLDEARA VGAEFNRIAG ENCLYFETGQ GSALSAGANF 310 320 330 340 350 360 GADQVTMEAR NYGLARHYDP FIVNTVVGFI GPEYLYNDRQ IIRAGLEDHF MGKLSGISMG 370 380 390 400 410 420 CDCCYTNHAD ADQNLNENLM ILLATAGCNY IMGMPLGDDI MLNYQTTAFH DTATVRQLLN 430 440 450 LRPSPEFERW LESMGIMANG RLTKRAGDPS LFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Cloning, sequencing, and expression of the genes encoding the adenosylcobalamin-dependent ethanolamine ammonia-lyase of Salmonella typhimurium." Faust L.R.P., Connor J.A., Roof D.M., Hoch J.A., Babior B.M. J. Biol. Chem. 265:12462-12466(1990) [PubMed: 2197274] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-30.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00096 Genomic DNA. Translation: AAC75494.2.
AP009048 Genomic DNA. Translation: BAA16323.1.

PIR

H65018.

RefSeq

NP_416936.4. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ABO	X-ray	2.10	A/C	1-453	[»]
3ABQ	X-ray	2.05	A/C	1-453	[»]
3ABR	X-ray	2.10	A/C	1-453	[»]
3ABS	X-ray	2.25	A/C	1-453	[»]
3ANY	X-ray	2.10	A/C	1-453	[»]
3AO0	X-ray	2.25	A/C	1-453	[»]

ProteinModelPortal

P0AEJ6.

SMR

P0AEJ6. Positions 1-453.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0AEJ6. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000001962; EBESCP00000001962; EBESCG00000001611.
EBESCT00000017009; EBESCP00000016300; EBESCG00000016068.

GeneID

946924.

GenomeReviews

Gene locus JW2434 in contig AP009048_GR.
Gene locus b2441 in contig U00096_GR.

KEGG

ecj:JW2434.
eco:b2441.

PATRIC

32120267. VBIEscCol129921_2536.

Organism-specific databases

EchoBASE

EB4299.

EcoGene

EG50006. eutB.

Phylogenomic databases

eggNOG

COG4303.

GeneTree

EBGT00050000009179.

HOGENOM

HBG289204.

OMA

IPYEEDC.

PhylomeDB

P0AEJ6.

ProtClustDB

PRK15067.

Enzyme and pathway databases

BioCyc

EcoCyc:EUTB-MONOMER.
MetaCyc:EUTB-MONOMER.

Gene expression databases

Genevestigator

P0AEJ6.

Family and domain databases

InterPro

IPR013785. Aldolase_TIM.
IPR010628. EutB.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

KO

K03735.

Pfam

PF06751. EutB. 1 hit.
[Graphical view]

PIRSF

PIRSF018788. EutB. 1 hit.

ProtoNet

Search...

Entry information

Entry name

EUTB_ECOLI

Accession

Primary (citable) accession number: P0AEJ6
Secondary accession number(s): P19635, P78094, P78300

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program