Isochorismate synthase entC - Escherichia coli (strain K12)

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P0AEJ2 (ENTC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 55. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Isochorismate synthase entC
EC=5.4.4.2

Alternative name(s):
Isochorismate mutase

Gene names

Name:	entC
Ordered Locus Names:	b0593, JW0585

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Chorismate = isochorismate.
Pathway	Siderophore biosynthesis; enterobactin biosynthesis.
Subunit structure	Monomer.
Induction	By iron starvation.
Sequence similarities	Belongs to the isochorismate synthase family.
Sequence caution	The sequence AAB40793.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Enterobactin biosynthesis
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	enterobactin biosynthetic process Inferred from mutant phenotype Ref.2. Source: EcoCyc
Molecular function	isochorismate synthase activity Inferred from direct assay Ref.7. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 391

391

Isochorismate synthase entC

PRO_0000154144

Experimental info

Sequence conflict

305 – 306

SG → TA in AAA18491. Ref.1

Secondary structure

391

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0AEJ2 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 62882569DFC41AC4
Show »

FASTA

391

42,932

        10         20         30         40         50         60 
MDTSLAEEVQ QTMATLAPNR FFFMSPYRSF TTSGCFARFD EPAVNGDSPD SPFQQKLAAL 

        70         80         90        100        110        120 
FADAKAQGIK NPVMVGAIPF DPRQPSSLYI PESWQSFSRQ EKQASARRFT RSQSLNVVER 

       130        140        150        160        170        180 
QAIPEQTTFE QMVARAAALT ATPQVDKVVL SRLIDITTDA AIDSGVLLER LIAQNPVSYN 

       190        200        210        220        230        240 
FHVPLADGGV LLGASPELLL RKDGERFSSI PLAGSARRQP DEVLDREAGN RLLASEKDRH 

       250        260        270        280        290        300 
EHELVTQAMK EVLRERSSEL HVPSSPQLIT TPTLWHLATP FEGKANSQEN ALTLACLLHP 

       310        320        330        340        350        360 
TPALSGFPHQ AATQVIAELE PFDRELFGGI VGWCDSEGNG EWVVTIRCAK LRENQVRLFA 

       370        380        390 
GAGIVPASSP LGEWRETGVK LSTMLNVFGL H

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Opacity factor from group A streptococci is an apoproteinase." Elkins M.F., Earhart C.F. FEMS Microbiol. Lett. 56:35-39(1990) [PubMed: 2110093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Nucleotide sequence of Escherichia coli isochorismate synthetase gene entC and evolutionary relationship of isochorismate synthetase and other chorismate-utilizing enzymes." Ozenberger B.A., Brickman T.J., McIntosh M.A. J. Bacteriol. 171:775-783(1989) [PubMed: 2536681] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Regulation of divergent transcription from the iron-responsive fepB-entC promoter-operator regions in Escherichia coli." Brickman T.J., Ozenberger B.A., McIntosh M.A. J. Mol. Biol. 212:669-682(1990) [PubMed: 2139473] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-33.
[7]	"Overexpression, purification, and characterization of isochorismate synthase (EntC), the first enzyme involved in the biosynthesis of enterobactin from chorismate." Liu J., Quinn N., Berchtold G.A., Walsh C.T. Biochemistry 29:1417-1425(1990) [PubMed: 2139795] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M36700 Genomic DNA. Translation: AAA18491.1.
M24142 Unassigned DNA. Translation: AAA16100.1.
U82598 Genomic DNA. Translation: AAB40793.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73694.1.
AP009048 Genomic DNA. Translation: BAE76348.1.
X53274 Genomic DNA. Translation: CAA37371.1.

PIR

SYECIK. JT0497.

RefSeq

NP_415125.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3HWO	X-ray	2.30	A/B	1-391	[»]

ProteinModelPortal

P0AEJ2.

SMR

P0AEJ2. Positions 13-391.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-47970N.

IntAct

P0AEJ2. 9 interactions.

MINT

MINT-1300552.

2D gel databases

2DBase-Ecoli

P0AEJ2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000004676; EBESCP00000004676; EBESCG00000003818.
EBESCT00000014653; EBESCP00000013944; EBESCG00000013714.

GeneID

945511.

GenomeReviews

Gene locus JW0585 in contig AP009048_GR.
Gene locus b0593 in contig U00096_GR.

KEGG

ecj:JW0585.
eco:b0593.

PATRIC

32116362. VBIEscCol129921_0621.

Organism-specific databases

EchoBASE

EB0257.

EcoGene

EG10261. entC.

Phylogenomic databases

eggNOG

COG1169.

GeneTree

EBGT00050000009519.

HOGENOM

HBG405459.

OMA

VTIRCAR.

PhylomeDB

P0AEJ2.

ProtClustDB

PRK15016.

Enzyme and pathway databases

BioCyc

EcoCyc:ENTC-MONOMER.
MetaCyc:ENTC-MONOMER.

Gene expression databases

Genevestigator

P0AEJ2.

Family and domain databases

InterPro

IPR005801. ADC_synthase.
IPR015890. Chorismate-bd_C.
IPR004561. IsoChor_synthase.
[Graphical view]

Gene3D

G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.

K02361.

PANTHER

PTHR11236:SF3. Isochor_synth. 1 hit.

Pfam

PF00425. Chorismate_bind. 1 hit.
[Graphical view]

SUPFAM

SSF56322. TRPE_1_chor_bd. 1 hit.

TIGRFAMs

TIGR00543. Isochor_syn. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ENTC_ECOLI

Accession

Primary (citable) accession number: P0AEJ2
Secondary accession number(s): P10377, P77099, Q2MBK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents