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Protein

Multidrug export protein EmrB

Gene

emrB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the tripartite efflux system EmrAB-TolC, which confers resistance to antibiotics such as CCCP, FCCP, 2,4-dinitrophenol and nalidixic acid.2 Publications

GO - Molecular functioni

GO - Biological processi

  • response to antibiotic Source: UniProtKB-KW
  • response to toxic substance Source: EcoCyc

Keywordsi

Biological processAntibiotic resistance, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EMRB-MONOMER

Protein family/group databases

TCDBi2.A.1.3.2 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug export protein EmrB
Gene namesi
Name:emrB
Ordered Locus Names:b2686, JW2661
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11439 emrB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 38HelicalSequence analysisAdd BLAST26
Topological domaini39 – 51ExtracellularSequence analysisAdd BLAST13
Transmembranei52 – 72HelicalSequence analysisAdd BLAST21
Topological domaini73 – 81CytoplasmicSequence analysis9
Transmembranei82 – 100HelicalSequence analysisAdd BLAST19
Topological domaini101 – 109ExtracellularSequence analysis9
Transmembranei110 – 128HelicalSequence analysisAdd BLAST19
Topological domaini129 – 136CytoplasmicSequence analysis8
Transmembranei137 – 159HelicalSequence analysisAdd BLAST23
Topological domaini160 – 164ExtracellularSequence analysis5
Transmembranei165 – 189HelicalSequence analysisAdd BLAST25
Topological domaini190 – 202CytoplasmicSequence analysisAdd BLAST13
Transmembranei203 – 223HelicalSequence analysisAdd BLAST21
Topological domaini224 – 233ExtracellularSequence analysis10
Transmembranei234 – 249HelicalSequence analysisAdd BLAST16
Topological domaini250 – 271CytoplasmicSequence analysisAdd BLAST22
Transmembranei272 – 295HelicalSequence analysisAdd BLAST24
Topological domaini296 – 305ExtracellularSequence analysis10
Transmembranei306 – 329HelicalSequence analysisAdd BLAST24
Topological domaini330 – 335CytoplasmicSequence analysis6
Transmembranei336 – 355HelicalSequence analysisAdd BLAST20
Topological domaini356 – 363ExtracellularSequence analysis8
Transmembranei364 – 387HelicalSequence analysisAdd BLAST24
Topological domaini388 – 407CytoplasmicSequence analysisAdd BLAST20
Transmembranei408 – 428HelicalSequence analysisAdd BLAST21
Topological domaini429 – 481ExtracellularSequence analysisAdd BLAST53
Transmembranei482 – 504HelicalSequence analysisAdd BLAST23
Topological domaini505 – 512CytoplasmicSequence analysis8

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001733221 – 512Multidrug export protein EmrBAdd BLAST512

Proteomic databases

PaxDbiP0AEJ0
PRIDEiP0AEJ0

Interactioni

Subunit structurei

Part of the tripartite efflux system EmrAB-TolC, which is composed of an inner membrane transporter, EmrB, a periplasmic membrane fusion protein, EmrA, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes. Interacts with EmrA. EmrAB complex forms a dimer in vitro.2 Publications

Protein-protein interaction databases

BioGridi4263179, 251 interactors
STRINGi316385.ECDH10B_2853

Structurei

3D structure databases

ProteinModelPortaliP0AEJ0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi505 – 510Poly-Gly6

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C0R Bacteria
ENOG410XNN3 LUCA
HOGENOMiHOG000112190
InParanoidiP0AEJ0
KOiK03446
OMAiSWLCGIS
PhylomeDBiP0AEJ0

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR004638 Drug-R_transpt_efflux_EmrB
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 3 hits
TIGRFAMsiTIGR00711 efflux_EmrB, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit

Sequencei

Sequence statusi: Complete.

P0AEJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQQKPLEGA QLVIMTIALS LATFMQVLDS TIANVAIPTI AGNLGSSLSQ
60 70 80 90 100
GTWVITSFGV ANAISIPLTG WLAKRVGEVK LFLWSTIAFA IASWACGVSS
110 120 130 140 150
SLNMLIFFRV IQGIVAGPLI PLSQSLLLNN YPPAKRSIAL ALWSMTVIVA
160 170 180 190 200
PICGPILGGY ISDNYHWGWI FFINVPIGVA VVLMTLQTLR GRETRTERRR
210 220 230 240 250
IDAVGLALLV IGIGSLQIML DRGKELDWFS SQEIIILTVV AVVAICFLIV
260 270 280 290 300
WELTDDNPIV DLSLFKSRNF TIGCLCISLA YMLYFGAIVL LPQLLQEVYG
310 320 330 340 350
YTATWAGLAS APVGIIPVIL SPIIGRFAHK LDMRRLVTFS FIMYAVCFYW
360 370 380 390 400
RAYTFEPGMD FGASAWPQFI QGFAVACFFM PLTTITLSGL PPERLAAASS
410 420 430 440 450
LSNFTRTLAG SIGTSITTTM WTNRESMHHA QLTESVNPFN PNAQAMYSQL
460 470 480 490 500
EGLGMTQQQA SGWIAQQITN QGLIISANEI FWMSAGIFLV LLGLVWFAKP
510
PFGAGGGGGG AH
Length:512
Mass (Da):55,624
Last modified:December 20, 2005 - v1
Checksum:i070334E68B303EFF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti325G → A in AAA23725 (PubMed:1409590).Curated1
Sequence conflicti501P → A in AAA23725 (PubMed:1409590).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86657 Genomic DNA Translation: AAA23725.1
U00096 Genomic DNA Translation: AAC75733.1
AP009048 Genomic DNA Translation: BAA16548.1
PIRiG65048
RefSeqiNP_417171.1, NC_000913.3
WP_001295176.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75733; AAC75733; b2686
BAA16548; BAA16548; BAA16548
GeneIDi947167
KEGGiecj:JW2661
eco:b2686
PATRICifig|1411691.4.peg.4053

Entry informationi

Entry nameiEMRB_ECOLI
AccessioniPrimary (citable) accession number: P0AEJ0
Secondary accession number(s): P27304, P77725
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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