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Protein

Phosphatase NudJ

Gene

nudJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of 4-amino-2-methyl-5-hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2-methyl-5-hydroxymethylpyrimidine phosphate (HMP-P), and hydrolysis of thiamine pyrophosphate (TPP) to thiamine monophosphate (TMP). Can hydrolyze other substrates such as MeO-HMP-PP, CF(3)-HMP-PP and MeO-TPP. Is also a non-specific nucleoside tri- and diphosphatase that releases inorganic orthophosphate.2 Publications

Cofactori

Mg2+1 Publication

Kineticsi

  1. KM=0.64 mM for GDP1 Publication
  1. Vmax=12 µmol/min/mg enzyme with GDP as substrate1 Publication

pH dependencei

Optimum pH is about 8.5.1 Publication

GO - Molecular functioni

  • nucleoside-diphosphatase activity Source: EcoCyc
  • nucleoside-triphosphatase activity Source: EcoCyc
  • thiamine-pyrophosphatase activity Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciEcoCyc:G6580-MONOMER.
ECOL316407:JW1120-MONOMER.
MetaCyc:G6580-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatase NudJ (EC:3.6.1.-)
Gene namesi
Name:nudJ
Synonyms:ymfB
Ordered Locus Names:b1134, JW1120
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13446. nudJ.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 153153Phosphatase NudJPRO_0000057069Add
BLAST

Proteomic databases

PaxDbiP0AEI6.
PRIDEiP0AEI6.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4263128. 44 interactions.
DIPiDIP-35908N.
IntActiP0AEI6. 5 interactions.
STRINGi511145.b1134.

Structurei

3D structure databases

ProteinModelPortaliP0AEI6.
SMRiP0AEI6. Positions 1-153.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 131129Nudix hydrolasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi36 – 5722Nudix boxAdd
BLAST

Sequence similaritiesi

Belongs to the Nudix hydrolase family. NudJ subfamily.Curated
Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105KB7. Bacteria.
COG0494. LUCA.
HOGENOMiHOG000256353.
InParanoidiP0AEI6.
KOiK12152.
OMAiRWKPNVT.
PhylomeDBiP0AEI6.

Family and domain databases

CDDicd03675. Nudix_Hydrolase_2. 1 hit.
Gene3Di3.90.79.10. 1 hit.
InterProiIPR020476. Nudix_hydrolase.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
IPR033713. NudJ.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
PRINTSiPR00502. NUDIXFAMILY.
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKPHVTVAC VVHAEGKFLV VEETINGKAL WNQPAGHLEA DETLVEAAAR
60 70 80 90 100
ELWEETGISA QPQHFIRMHQ WIAPDKTPFL RFLFAIELEQ ICPTQPHDSD
110 120 130 140 150
IDCCRWVSAE EILQASNLRS PLVAESIRCY QSGQRYPLEM IGDFNWPFTK

GVI
Length:153
Mass (Da):17,433
Last modified:December 6, 2005 - v1
Checksum:iBDB7423E97D6735A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74218.1.
AP009048 Genomic DNA. Translation: BAA35956.1.
PIRiC64858.
RefSeqiNP_415652.1. NC_000913.3.
WP_000476093.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74218; AAC74218; b1134.
BAA35956; BAA35956; BAA35956.
GeneIDi945689.
KEGGiecj:JW1120.
eco:b1134.
PATRICi32117517. VBIEscCol129921_1181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74218.1.
AP009048 Genomic DNA. Translation: BAA35956.1.
PIRiC64858.
RefSeqiNP_415652.1. NC_000913.3.
WP_000476093.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AEI6.
SMRiP0AEI6. Positions 1-153.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263128. 44 interactions.
DIPiDIP-35908N.
IntActiP0AEI6. 5 interactions.
STRINGi511145.b1134.

Proteomic databases

PaxDbiP0AEI6.
PRIDEiP0AEI6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74218; AAC74218; b1134.
BAA35956; BAA35956; BAA35956.
GeneIDi945689.
KEGGiecj:JW1120.
eco:b1134.
PATRICi32117517. VBIEscCol129921_1181.

Organism-specific databases

EchoBASEiEB3220.
EcoGeneiEG13446. nudJ.

Phylogenomic databases

eggNOGiENOG4105KB7. Bacteria.
COG0494. LUCA.
HOGENOMiHOG000256353.
InParanoidiP0AEI6.
KOiK12152.
OMAiRWKPNVT.
PhylomeDBiP0AEI6.

Enzyme and pathway databases

BioCyciEcoCyc:G6580-MONOMER.
ECOL316407:JW1120-MONOMER.
MetaCyc:G6580-MONOMER.

Miscellaneous databases

PROiP0AEI6.

Family and domain databases

CDDicd03675. Nudix_Hydrolase_2. 1 hit.
Gene3Di3.90.79.10. 1 hit.
InterProiIPR020476. Nudix_hydrolase.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
IPR033713. NudJ.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
PRINTSiPR00502. NUDIXFAMILY.
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUDJ_ECOLI
AccessioniPrimary (citable) accession number: P0AEI6
Secondary accession number(s): P75965
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: September 7, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.