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Protein

Ribosomal protein S12 methylthiotransferase RimO

Gene

rimO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the methylthiolation of the residue Asp-89 of ribosomal protein S12.2 Publications

Catalytic activityi

L-aspartate-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthio-L-aspartate-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.1 Publication

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi17Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi53Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi82Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi150Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi154Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi157Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • aspartic acid methylthiotransferase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • protein methylthiotransferase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G6435-MONOMER
MetaCyc:G6435-MONOMER
BRENDAi2.8.4.4 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S12 methylthiotransferase RimO (EC:2.8.4.41 Publication)
Short name:
S12 MTTase
Short name:
S12 methylthiotransferase
Alternative name(s):
Ribosomal protein S12 (aspartate(89)-C(3))-methylthiotransferase
Ribosome maturation factor RimO
Gene namesi
Name:rimO
Synonyms:yliG
Ordered Locus Names:b0835, JW0819
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13478 rimO

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutant shows a lack of methylthiolation of protein S12 and a decrease in transcription of a subset of genes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001417381 – 441Ribosomal protein S12 methylthiotransferase RimOAdd BLAST441

Proteomic databases

PaxDbiP0AEI4
PRIDEiP0AEI4

Interactioni

Protein-protein interaction databases

BioGridi4261845, 65 interactors
DIPiDIP-48218N
IntActiP0AEI4, 20 interactors
STRINGi316385.ECDH10B_0904

Structurei

3D structure databases

ProteinModelPortaliP0AEI4
SMRiP0AEI4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 118MTTase N-terminalAdd BLAST111
Domaini376 – 441TRAMAdd BLAST66

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CBM Bacteria
COG0621 LUCA
HOGENOMiHOG000224766
InParanoidiP0AEI4
KOiK14441
OMAiCLSERYK
PhylomeDBiP0AEI4

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01865 MTTase_RimO, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR005840 Ribosomal_S12_MeSTrfase_RimO
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PANTHERiPTHR43837 PTHR43837, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00274 ribosomal_protein_S12_methylth, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
TIGR01125 TIGR01125, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

P0AEI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVTPQPKI GFVSLGCPKN LVDSERILTE LRTEGYDVVP SYDDADMVIV
60 70 80 90 100
NTCGFIDSAV QESLEAIGEA LNENGKVIVT GCLGAKEDQI REVHPKVLEI
110 120 130 140 150
TGPHSYEQVL EHVHHYVPKP KHNPFLSLVP EQGVKLTPRH YAYLKISEGC
160 170 180 190 200
NHRCTFCIIP SMRGDLVSRP IGEVLSEAKR LVDAGVKEIL VISQDTSAYG
210 220 230 240 250
VDVKHRTGFH NGEPVKTSMV SLCEQLSKLG IWTRLHYVYP YPHVDDVIPL
260 270 280 290 300
MAEGKILPYL DIPLQHASPR ILKLMKRPGS VDRQLARIKQ WREICPELTL
310 320 330 340 350
RSTFIVGFPG ETEEDFQMLL DFLKEARLDR VGCFKYSPVE GADANALPDQ
360 370 380 390 400
VPEEVKEERW NRFMQLQQQI SAERLQEKVG REILVIIDEV DEEGAIGRSM
410 420 430 440
ADAPEIDGAV YLNGETNVKP GDILRVKVEH ADEYDLWGSR V
Length:441
Mass (Da):49,582
Last modified:December 6, 2005 - v1
Checksum:iE1D4605388AFB889
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC73922.1
AP009048 Genomic DNA Translation: BAA35530.1
PIRiC64821
RefSeqiNP_415356.1, NC_000913.3
WP_000049367.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73922; AAC73922; b0835
BAA35530; BAA35530; BAA35530
GeneIDi945465
KEGGiecj:JW0819
eco:b0835
PATRICifig|1411691.4.peg.1443

Similar proteinsi

Entry informationi

Entry nameiRIMO_ECOLI
AccessioniPrimary (citable) accession number: P0AEI4
Secondary accession number(s): P75802
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health