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Protein

Ribosomal protein S12 methylthiotransferase RimO

Gene

rimO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methylthiolation of the residue Asp-89 of ribosomal protein S12.2 Publications

Catalytic activityi

L-aspartate-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthio-L-aspartate-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.1 Publication

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi17 – 171Iron-sulfur (4Fe-4S)By similarity
Metal bindingi53 – 531Iron-sulfur (4Fe-4S)By similarity
Metal bindingi82 – 821Iron-sulfur (4Fe-4S)By similarity
Metal bindingi150 – 1501Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi154 – 1541Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi157 – 1571Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • aspartic acid methylthiotransferase activity Source: EcoCyc
  • iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G6435-MONOMER.
ECOL316407:JW0819-MONOMER.
MetaCyc:G6435-MONOMER.
BRENDAi2.8.4.4. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S12 methylthiotransferase RimO (EC:2.8.4.41 Publication)
Short name:
S12 MTTase
Short name:
S12 methylthiotransferase
Alternative name(s):
Ribosomal protein S12 (aspartate(89)-C(3))-methylthiotransferase
Ribosome maturation factor RimO
Gene namesi
Name:rimO
Synonyms:yliG
Ordered Locus Names:b0835, JW0819
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13478. rimO.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutant shows a lack of methylthiolation of protein S12 and a decrease in transcription of a subset of genes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 441441Ribosomal protein S12 methylthiotransferase RimOPRO_0000141738Add
BLAST

Proteomic databases

PaxDbiP0AEI4.
PRIDEiP0AEI4.

Interactioni

Protein-protein interaction databases

BioGridi4261845. 6 interactions.
DIPiDIP-48218N.
IntActiP0AEI4. 20 interactions.
STRINGi511145.b0835.

Structurei

3D structure databases

ProteinModelPortaliP0AEI4.
SMRiP0AEI4. Positions 10-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 118111MTTase N-terminalAdd
BLAST
Domaini376 – 44166TRAMAdd
BLAST

Sequence similaritiesi

Contains 1 MTTase N-terminal domain.Curated
Contains 1 TRAM domain.Curated

Phylogenomic databases

eggNOGiENOG4105CBM. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224766.
InParanoidiP0AEI4.
KOiK14441.
OMAiHYAYPTG.
PhylomeDBiP0AEI4.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.80.30.20. 1 hit.
HAMAPiMF_01865. MTTase_RimO. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR012340. NA-bd_OB-fold.
IPR005840. Ribosomal_S12_MeSTrfase_RimO.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
TIGR01125. TIGR01125. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVTPQPKI GFVSLGCPKN LVDSERILTE LRTEGYDVVP SYDDADMVIV
60 70 80 90 100
NTCGFIDSAV QESLEAIGEA LNENGKVIVT GCLGAKEDQI REVHPKVLEI
110 120 130 140 150
TGPHSYEQVL EHVHHYVPKP KHNPFLSLVP EQGVKLTPRH YAYLKISEGC
160 170 180 190 200
NHRCTFCIIP SMRGDLVSRP IGEVLSEAKR LVDAGVKEIL VISQDTSAYG
210 220 230 240 250
VDVKHRTGFH NGEPVKTSMV SLCEQLSKLG IWTRLHYVYP YPHVDDVIPL
260 270 280 290 300
MAEGKILPYL DIPLQHASPR ILKLMKRPGS VDRQLARIKQ WREICPELTL
310 320 330 340 350
RSTFIVGFPG ETEEDFQMLL DFLKEARLDR VGCFKYSPVE GADANALPDQ
360 370 380 390 400
VPEEVKEERW NRFMQLQQQI SAERLQEKVG REILVIIDEV DEEGAIGRSM
410 420 430 440
ADAPEIDGAV YLNGETNVKP GDILRVKVEH ADEYDLWGSR V
Length:441
Mass (Da):49,582
Last modified:December 6, 2005 - v1
Checksum:iE1D4605388AFB889
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73922.1.
AP009048 Genomic DNA. Translation: BAA35530.1.
PIRiC64821.
RefSeqiNP_415356.1. NC_000913.3.
WP_000049367.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73922; AAC73922; b0835.
BAA35530; BAA35530; BAA35530.
GeneIDi945465.
KEGGiecj:JW0819.
eco:b0835.
PATRICi32116873. VBIEscCol129921_0862.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73922.1.
AP009048 Genomic DNA. Translation: BAA35530.1.
PIRiC64821.
RefSeqiNP_415356.1. NC_000913.3.
WP_000049367.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AEI4.
SMRiP0AEI4. Positions 10-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261845. 6 interactions.
DIPiDIP-48218N.
IntActiP0AEI4. 20 interactions.
STRINGi511145.b0835.

Proteomic databases

PaxDbiP0AEI4.
PRIDEiP0AEI4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73922; AAC73922; b0835.
BAA35530; BAA35530; BAA35530.
GeneIDi945465.
KEGGiecj:JW0819.
eco:b0835.
PATRICi32116873. VBIEscCol129921_0862.

Organism-specific databases

EchoBASEiEB3251.
EcoGeneiEG13478. rimO.

Phylogenomic databases

eggNOGiENOG4105CBM. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224766.
InParanoidiP0AEI4.
KOiK14441.
OMAiHYAYPTG.
PhylomeDBiP0AEI4.

Enzyme and pathway databases

BioCyciEcoCyc:G6435-MONOMER.
ECOL316407:JW0819-MONOMER.
MetaCyc:G6435-MONOMER.
BRENDAi2.8.4.4. 2026.

Miscellaneous databases

PROiP0AEI4.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.80.30.20. 1 hit.
HAMAPiMF_01865. MTTase_RimO. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR012340. NA-bd_OB-fold.
IPR005840. Ribosomal_S12_MeSTrfase_RimO.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
TIGR01125. TIGR01125. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIMO_ECOLI
AccessioniPrimary (citable) accession number: P0AEI4
Secondary accession number(s): P75802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.