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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation1 Publication

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi12 – 121Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi157 – 1571Iron-sulfur (4Fe-4S-S-AdoMet)Curated
Metal bindingi161 – 1611Iron-sulfur (4Fe-4S-S-AdoMet)Curated
Metal bindingi164 – 1641Iron-sulfur (4Fe-4S-S-AdoMet)Curated

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • iron ion binding Source: UniProtKB-HAMAP
  • N6-isopentenyladenosine methylthiotransferase activity Source: EcoCyc

GO - Biological processi

  • tRNA methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G6364-MONOMER.
ECOL316407:JW0658-MONOMER.
MetaCyc:G6364-MONOMER.
BRENDAi2.8.4.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Synonyms:yleA
Ordered Locus Names:b0661, JW0658
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13657. miaB.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi157 – 1571C → A: Loss of activity. 1 Publication
Mutagenesisi161 – 1611C → A: Loss of activity. 1 Publication
Mutagenesisi164 – 1641C → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474tRNA-2-methylthio-N(6)-dimethylallyladenosine synthasePRO_0000141734Add
BLAST

Proteomic databases

PaxDbiP0AEI1.
PRIDEiP0AEI1.

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4261232. 10 interactions.
DIPiDIP-48018N.
IntActiP0AEI1. 13 interactions.
MINTiMINT-1233637.
STRINGi511145.b0661.

Structurei

3D structure databases

ProteinModelPortaliP0AEI1.
SMRiP0AEI1. Positions 4-440.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 120118MTTase N-terminalUniRule annotationAdd
BLAST
Domaini378 – 44164TRAMUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
Contains 1 MTTase N-terminal domain.UniRule annotation
Contains 1 TRAM domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224767.
InParanoidiP0AEI1.
KOiK06168.
OMAiIPMDLIL.
PhylomeDBiP0AEI1.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKLHIKTW GCQMNEYDSS KMADLLDATH GYQLTDVAEE ADVLLLNTCS
60 70 80 90 100
IREKAQEKVF HQLGRWKLLK EKNPDLIIGV GGCVASQEGE HIRQRAHYVD
110 120 130 140 150
IIFGPQTLHR LPEMINSVRG DRSPVVDISF PEIEKFDRLP EPRAEGPTAF
160 170 180 190 200
VSIMEGCNKY CTYCVVPYTR GEEVSRPSDD ILFEIAQLAA QGVREVNLLG
210 220 230 240 250
QNVNAWRGEN YDGTTGSFAD LLRLVAAIDG IDRIRFTTSH PIEFTDDIIE
260 270 280 290 300
VYRDTPELVS FLHLPVQSGS DRILNLMGRT HTALEYKAII RKLRAARPDI
310 320 330 340 350
QISSDFIVGF PGETTEDFEK TMKLIADVNF DMSYSFIFSA RPGTPAADMV
360 370 380 390 400
DDVPEEEKKQ RLYILQERIN QQAMAWSRRM LGTTQRILVE GTSRKSIMEL
410 420 430 440 450
SGRTENNRVV NFEGTPDMIG KFVDVEITDV YPNSLRGKVV RTEDEMGLRV
460 470
AETPESVIAR TRKENDLGVG YYQP
Length:474
Mass (Da):53,663
Last modified:December 6, 2005 - v1
Checksum:i49CBCB93888F7F9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40863.1.
U00096 Genomic DNA. Translation: AAC73762.1.
AP009048 Genomic DNA. Translation: BAE76357.1.
PIRiC64801.
RefSeqiNP_415194.1. NC_000913.3.
WP_000162740.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73762; AAC73762; b0661.
BAE76357; BAE76357; BAE76357.
GeneIDi945260.
KEGGiecj:JW0658.
eco:b0661.
PATRICi32116511. VBIEscCol129921_0695.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40863.1.
U00096 Genomic DNA. Translation: AAC73762.1.
AP009048 Genomic DNA. Translation: BAE76357.1.
PIRiC64801.
RefSeqiNP_415194.1. NC_000913.3.
WP_000162740.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AEI1.
SMRiP0AEI1. Positions 4-440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261232. 10 interactions.
DIPiDIP-48018N.
IntActiP0AEI1. 13 interactions.
MINTiMINT-1233637.
STRINGi511145.b0661.

Proteomic databases

PaxDbiP0AEI1.
PRIDEiP0AEI1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73762; AAC73762; b0661.
BAE76357; BAE76357; BAE76357.
GeneIDi945260.
KEGGiecj:JW0658.
eco:b0661.
PATRICi32116511. VBIEscCol129921_0695.

Organism-specific databases

EchoBASEiEB3421.
EcoGeneiEG13657. miaB.

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224767.
InParanoidiP0AEI1.
KOiK06168.
OMAiIPMDLIL.
PhylomeDBiP0AEI1.

Enzyme and pathway databases

BioCyciEcoCyc:G6364-MONOMER.
ECOL316407:JW0658-MONOMER.
MetaCyc:G6364-MONOMER.
BRENDAi2.8.4.3. 2026.

Miscellaneous databases

PROiP0AEI1.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMIAB_ECOLI
AccessioniPrimary (citable) accession number: P0AEI1
Secondary accession number(s): P77645, Q2MBJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.