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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation1 Publication

Catalytic activityi

N6-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N6-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi83Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi157Iron-sulfur (4Fe-4S-S-AdoMet)Curated1
Metal bindingi161Iron-sulfur (4Fe-4S-S-AdoMet)Curated1
Metal bindingi164Iron-sulfur (4Fe-4S-S-AdoMet)Curated1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • N6-isopentenyladenosine methylthiotransferase activity Source: EcoCyc

GO - Biological processi

  • tRNA methylation Source: EcoCyc

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G6364-MONOMER
MetaCyc:G6364-MONOMER
BRENDAi2.8.4.3 2026

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Synonyms:yleA
Ordered Locus Names:b0661, JW0658
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13657 miaB

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi157C → A: Loss of activity. 1 Publication1
Mutagenesisi161C → A: Loss of activity. 1 Publication1
Mutagenesisi164C → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001417341 – 474tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST474

Proteomic databases

PaxDbiP0AEI1
PRIDEiP0AEI1

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4261232, 46 interactors
DIPiDIP-48018N
IntActiP0AEI1, 15 interactors
STRINGi316385.ECDH10B_0730

Structurei

3D structure databases

ProteinModelPortaliP0AEI1
SMRiP0AEI1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 120MTTase N-terminalUniRule annotationAdd BLAST118
Domaini378 – 441TRAMUniRule annotationAdd BLAST64

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW Bacteria
COG0621 LUCA
HOGENOMiHOG000224767
InParanoidiP0AEI1
KOiK06168
OMAiKVCEHFH
PhylomeDBiP0AEI1

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01864 tRNA_metthiotr_MiaB, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR006463 MiaB_methiolase
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF01938 TRAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00273 (dimethylallyl)adenosine_tRNA_, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

P0AEI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKLHIKTW GCQMNEYDSS KMADLLDATH GYQLTDVAEE ADVLLLNTCS
60 70 80 90 100
IREKAQEKVF HQLGRWKLLK EKNPDLIIGV GGCVASQEGE HIRQRAHYVD
110 120 130 140 150
IIFGPQTLHR LPEMINSVRG DRSPVVDISF PEIEKFDRLP EPRAEGPTAF
160 170 180 190 200
VSIMEGCNKY CTYCVVPYTR GEEVSRPSDD ILFEIAQLAA QGVREVNLLG
210 220 230 240 250
QNVNAWRGEN YDGTTGSFAD LLRLVAAIDG IDRIRFTTSH PIEFTDDIIE
260 270 280 290 300
VYRDTPELVS FLHLPVQSGS DRILNLMGRT HTALEYKAII RKLRAARPDI
310 320 330 340 350
QISSDFIVGF PGETTEDFEK TMKLIADVNF DMSYSFIFSA RPGTPAADMV
360 370 380 390 400
DDVPEEEKKQ RLYILQERIN QQAMAWSRRM LGTTQRILVE GTSRKSIMEL
410 420 430 440 450
SGRTENNRVV NFEGTPDMIG KFVDVEITDV YPNSLRGKVV RTEDEMGLRV
460 470
AETPESVIAR TRKENDLGVG YYQP
Length:474
Mass (Da):53,663
Last modified:December 6, 2005 - v1
Checksum:i49CBCB93888F7F9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA Translation: AAB40863.1
U00096 Genomic DNA Translation: AAC73762.1
AP009048 Genomic DNA Translation: BAE76357.1
PIRiC64801
RefSeqiNP_415194.1, NC_000913.3
WP_000162740.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73762; AAC73762; b0661
BAE76357; BAE76357; BAE76357
GeneIDi945260
KEGGiecj:JW0658
eco:b0661
PATRICifig|1411691.4.peg.1607

Similar proteinsi

Entry informationi

Entry nameiMIAB_ECOLI
AccessioniPrimary (citable) accession number: P0AEI1
Secondary accession number(s): P77645, Q2MBJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

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