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Reviewed, UniProtKB/Swiss-Prot P0AEI1 (MIAB_ECOLI)

Last modified November 24, 2009. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    (Dimethylallyl)adenosine tRNA methylthiotransferase miaB
    EC=2.-.-.-
Alternative name(s):
    tRNA-i(6)A37 methylthiotransferase
Gene names
Name: miaB
Synonyms: yleA
Ordered Locus Names: b0661, JW0658
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine. Ref.4

Cofactor

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine Probable.

Subunit structure

Monomer. Ref.5

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Contains 1 MTTase N-terminal domain.

Contains 1 TRAM domain.

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Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtRNA modification

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: HAMAP

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

transferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 474474(Dimethylallyl)adenosine tRNA methylthiotransferase miaB HAMAP MF_01864
PRO_0000141734

Regions

Domain3 – 120118MTTase N-terminal
Domain378 – 44164TRAM

Sites

Metal binding121Iron-sulfur (4Fe-4S) By similarity
Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity
Metal binding1571Iron-sulfur (4Fe-4S-S-AdoMet) Probable
Metal binding1611Iron-sulfur (4Fe-4S-S-AdoMet) Probable
Metal binding1641Iron-sulfur (4Fe-4S-S-AdoMet) Probable

Experimental info

Mutagenesis1571C → A: Loss of activity. Ref.5
Mutagenesis1611C → A: Loss of activity. Ref.5
Mutagenesis1641C → A: Loss of activity. Ref.5

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Sequences

Sequence LengthMass (Da)Tools
P0AEI1-1 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 49CBCB93888F7F9D

FASTA47453,663
        10         20         30         40         50         60 
MTKKLHIKTW GCQMNEYDSS KMADLLDATH GYQLTDVAEE ADVLLLNTCS IREKAQEKVF 

        70         80         90        100        110        120 
HQLGRWKLLK EKNPDLIIGV GGCVASQEGE HIRQRAHYVD IIFGPQTLHR LPEMINSVRG 

       130        140        150        160        170        180 
DRSPVVDISF PEIEKFDRLP EPRAEGPTAF VSIMEGCNKY CTYCVVPYTR GEEVSRPSDD 

       190        200        210        220        230        240 
ILFEIAQLAA QGVREVNLLG QNVNAWRGEN YDGTTGSFAD LLRLVAAIDG IDRIRFTTSH 

       250        260        270        280        290        300 
PIEFTDDIIE VYRDTPELVS FLHLPVQSGS DRILNLMGRT HTALEYKAII RKLRAARPDI 

       310        320        330        340        350        360 
QISSDFIVGF PGETTEDFEK TMKLIADVNF DMSYSFIFSA RPGTPAADMV DDVPEEEKKQ 

       370        380        390        400        410        420 
RLYILQERIN QQAMAWSRRM LGTTQRILVE GTSRKSIMEL SGRTENNRVV NFEGTPDMIG 

       430        440        450        460        470 
KFVDVEITDV YPNSLRGKVV RTEDEMGLRV AETPESVIAR TRKENDLGVG YYQP

« Hide

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References

« Hide 'large scale' references
[1]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Identification of the miaB gene, involved in methylthiolation of isopentenylated A37 derivatives in the tRNA of Salmonella typhimurium and Escherichia coli."
Esberg B., Leung H.-C.E., Tsui H.-C.T., Bjoerk G.R., Winkler M.E.
J. Bacteriol. 181:7256-7265(1999) [PubMed: 10572129] [Abstract]
Cited for: FUNCTION.
[5]"Enzymatic modification of tRNAs: MiaB is an iron-sulfur protein."
Pierrel F., Bjoerk G.R., Fontecave M., Atta M.
J. Biol. Chem. 277:13367-13370(2002) [PubMed: 11882645] [Abstract]
Cited for: COFACTOR, SUBUNIT, MUTAGENESIS OF CYS-157; CYS-161 AND CYS-164.

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Cross-references

Sequence databases

U82598 Genomic DNA. Translation: AAB40863.1.
U00096 Genomic DNA. Translation: AAC73762.1.
AP009048 Genomic DNA. Translation: BAE76357.1.
PIRC64801.
RefSeqAP_001310.1.
NP_415194.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGP0AEI1.

Proteomic databases

PRIDEP0AEI1.

Genome annotation databases

GeneID945260.
GenomeReviewsGene locus JW0658 in contig AP009048_GR.
Gene locus b0661 in contig U00096_GR.
KEGGecj:JW0658.
eco:b0661.

Organism-specific databases

EchoBASEEB3421.
EcoGeneEG13657. miaB.
CMRSearch...

Phylogenomic databases

HOGENOMP0AEI1.
OMAAANLHDD

Enzyme and pathway databases

BioCycEcoCyc:G6364-MON.
ECOL168927:B0661-MON.
MetaCyc:G6364-MON.

Gene expression databases

GenevestigatorP0AEI1.

Family and domain databases

HAMAPMF_01864.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. Radical_SAM.
IPR002792. TRAM.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHERPTHR11918. UPF0004. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR01574. miaB-methiolase. 1 hit.
TIGR00089. UPF0004. 1 hit.
PROSITEPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMIAB_ECOLI
AccessionPrimary (citable) accession number: P0AEI1
Secondary accession number(s): P77645, Q2MBJ9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 24, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents