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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation1 Publication

Catalytic activityi

N6-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N6-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi83Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi157Iron-sulfur (4Fe-4S-S-AdoMet)Curated1
Metal bindingi161Iron-sulfur (4Fe-4S-S-AdoMet)Curated1
Metal bindingi164Iron-sulfur (4Fe-4S-S-AdoMet)Curated1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • N6-isopentenyladenosine methylthiotransferase activity Source: EcoCyc

GO - Biological processi

  • tRNA methylation Source: EcoCyc

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G6364-MONOMER.
MetaCyc:G6364-MONOMER.
BRENDAi2.8.4.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Synonyms:yleA
Ordered Locus Names:b0661, JW0658
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13657. miaB.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi157C → A: Loss of activity. 1 Publication1
Mutagenesisi161C → A: Loss of activity. 1 Publication1
Mutagenesisi164C → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001417341 – 474tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST474

Proteomic databases

PaxDbiP0AEI1.
PRIDEiP0AEI1.

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4261232. 34 interactors.
DIPiDIP-48018N.
IntActiP0AEI1. 13 interactors.
MINTiMINT-1233637.
STRINGi316385.ECDH10B_0730.

Structurei

3D structure databases

ProteinModelPortaliP0AEI1.
SMRiP0AEI1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 120MTTase N-terminalUniRule annotationAdd BLAST118
Domaini378 – 441TRAMUniRule annotationAdd BLAST64

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224767.
InParanoidiP0AEI1.
KOiK06168.
PhylomeDBiP0AEI1.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiView protein in InterPro
IPR006638. Elp3/MiaB/NifB.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
PfamiView protein in Pfam
PF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
SFLDiSFLDF00273. (dimethylallyl)adenosine_tRNA_. 1 hit.
SFLDG01061. methylthiotransferase. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiView protein in PROSITE
PS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AEI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKLHIKTW GCQMNEYDSS KMADLLDATH GYQLTDVAEE ADVLLLNTCS
60 70 80 90 100
IREKAQEKVF HQLGRWKLLK EKNPDLIIGV GGCVASQEGE HIRQRAHYVD
110 120 130 140 150
IIFGPQTLHR LPEMINSVRG DRSPVVDISF PEIEKFDRLP EPRAEGPTAF
160 170 180 190 200
VSIMEGCNKY CTYCVVPYTR GEEVSRPSDD ILFEIAQLAA QGVREVNLLG
210 220 230 240 250
QNVNAWRGEN YDGTTGSFAD LLRLVAAIDG IDRIRFTTSH PIEFTDDIIE
260 270 280 290 300
VYRDTPELVS FLHLPVQSGS DRILNLMGRT HTALEYKAII RKLRAARPDI
310 320 330 340 350
QISSDFIVGF PGETTEDFEK TMKLIADVNF DMSYSFIFSA RPGTPAADMV
360 370 380 390 400
DDVPEEEKKQ RLYILQERIN QQAMAWSRRM LGTTQRILVE GTSRKSIMEL
410 420 430 440 450
SGRTENNRVV NFEGTPDMIG KFVDVEITDV YPNSLRGKVV RTEDEMGLRV
460 470
AETPESVIAR TRKENDLGVG YYQP
Length:474
Mass (Da):53,663
Last modified:December 6, 2005 - v1
Checksum:i49CBCB93888F7F9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40863.1.
U00096 Genomic DNA. Translation: AAC73762.1.
AP009048 Genomic DNA. Translation: BAE76357.1.
PIRiC64801.
RefSeqiNP_415194.1. NC_000913.3.
WP_000162740.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73762; AAC73762; b0661.
BAE76357; BAE76357; BAE76357.
GeneIDi945260.
KEGGiecj:JW0658.
eco:b0661.
PATRICifig|1411691.4.peg.1607.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMIAB_ECOLI
AccessioniPrimary (citable) accession number: P0AEI1
Secondary accession number(s): P77645, Q2MBJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: July 5, 2017
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries