Reviewed, UniProtKB/Swiss-Prot P0AEG7 (DSBC_ECO57)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbC

Gene names

Name:	dsbC
Ordered Locus Names:	Z4231, ECs3765

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbC is reoxidized by a yet uncharacterized protein. Also acts as a disulfide isomerase By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the thioredoxin family. DsbC subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Redox-active center Signal
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	By similarity
	Chain	21 – 236	216	Thiol:disulfide interchange protein dsbC		PRO_0000043368
Regions
	Domain	36 – 231	196	Thioredoxin
Amino acid modifications
	Disulfide bond	118 ↔ 121		Redox-active By similarity
	Disulfide bond	161 ↔ 183		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AEG7-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 69A834A666BAA6D6	FASTA	236	25,622
	10 20 30 40 50 60 MKKGFMLFTL LAAFSGFAQA DDAAIQQTLA KMGIKSSDIQ PAPVAGMKTV LTNSGVLYIT 70 80 90 100 110 120 DDGKHIIQGP MYDVSGTAPV NVTNKMLLKQ LNALEKEMIV YKAPQEKHVI TVFTDITCGY 130 140 150 160 170 180 CHKLHEQMAD YNALGITVRY LAFPRQGLDS DAEKEMKAIW CAKDKNKAFD DVMAGKSVAP 190 200 210 220 230 ASCDVDIADH YALGVQLGVS GTPAVVLSNG TLVPGYQPPK EMKEFLDEHQ KMTSGK

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG58021.1. BA000007 Genomic DNA. Translation: BAB37188.1.
PIR	E91099.
RefSeq	NP_289462.1. NP_311792.1.
3D structure databases
SMR	P0AEG7. Positions 21-236.
ModBase	Search...
Genome annotation databases
GeneID	916411. 958374.
GenomeReviews	Gene locus Z4231 in contig AE005174_GR. Gene locus ECs3765 in contig BA000007_GR.
KEGG	ece:Z4231. ecs:ECs3765.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0AEG7.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS3765-MON.
Family and domain databases
InterPro	IPR018950. dS-bond_isomerase_DsbC/G_N. IPR009094. dS-bond_isomerase_DsbC_N. IPR017936. Thioredoxin-like. IPR017937. Thioredoxin_CS. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.10.450.70. Disulf_isomers_N. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF10411. DsbC_N. 1 hit. [Graphical view]
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSBC_ECO57

Accession

Primary (citable) accession number: P0AEG7
Secondary accession number(s): P21892

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)