Reviewed, UniProtKB/Swiss-Prot P0AEG5 (DSBA_ECO57)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbA

Gene names

Name:	dsbA
Ordered Locus Names:	Z5392, ECs4783

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required for disulfide bond formation in some periplasmic proteins such as phoA or ompA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by dsbB. It is required for pilus biogenesis By similarity.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the thioredoxin family. DsbA subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Redox-active center Signal
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity
	Chain	20 – 208	189	Thiol:disulfide interchange protein dsbA		PRO_0000043367
Regions
	Domain	20 – 150	131	Thioredoxin
Amino acid modifications
	Disulfide bond	49 ↔ 52		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AEG5-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 10527E876FCAEE55	FASTA	208	23,105
	10 20 30 40 50 60 MKKIWLALAG LVLAFSASAA QYEDGKQYTT LEKPVAGAPQ VLEFFSFFCP HCYQFEEVLH 70 80 90 100 110 120 ISDNVKKKLP EGVKMTKYHV NFMGGDLGKD LTQAWAVAMA LGVEDKVTVP LFEGVQKTQT 130 140 150 160 170 180 IRSASDIRDV FINAGIKGEE YDAAWNSFVV KSLVAQQEKA AADVQLRGVP AMFVNGKYQL 190 200 NPQGMDTSNM DVFVQQYADT VKYLSEKK

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG59049.1. BA000007 Genomic DNA. Translation: BAB38206.1.
PIR	E86073. G91226.
RefSeq	NP_290485.1. NP_312810.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	915115. 960300.
GenomeReviews	Gene locus Z5392 in contig AE005174_GR. Gene locus ECs4783 in contig BA000007_GR.
KEGG	ece:Z5392. ecs:ECs4783.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0AEG5.
OMA	P0AEG5. EGVHYEV.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS4783-MON.
Family and domain databases
InterPro	IPR001853. OxRdtase_DSBA. IPR017936. Thioredoxin-like. IPR017937. Thioredoxin_CS. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF01323. DSBA. 1 hit. [Graphical view]
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSBA_ECO57

Accession

Primary (citable) accession number: P0AEG5
Secondary accession number(s): P24991, Q46951, Q46952

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)