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Protein

Transcriptional regulatory protein DpiA

Gene

dpiA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox conditions. Regulates the transcription of citCDEFXGT, dpiAB, mdh and exuT. Binds specifically to the dpiB-citC intergenic region.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi180 – 19920H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • nucleic acid binding transcription factor activity Source: InterPro
  • phosphorelay response regulator activity Source: EcoCyc

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • regulation of transcription, DNA-templated Source: EcoCyc
  • response to external stimulus Source: InterPro
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6346-MONOMER.
ECOL316407:JW0612-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein DpiA
Alternative name(s):
Destabilizer of plasmid inheritance
Gene namesi
Name:dpiA
Synonyms:citB, criR, mpdA
Ordered Locus Names:b0620, JW0612
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13544. dpiA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226Transcriptional regulatory protein DpiAPRO_0000081068Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei57 – 5714-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated and activated by DpiB.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AEF4.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
paaCP760794EBI-1119284,EBI-1131666
yjjWP394092EBI-1119284,EBI-9132384

Protein-protein interaction databases

BioGridi4263364. 6 interactions.
DIPiDIP-47831N.
IntActiP0AEF4. 15 interactions.
MINTiMINT-1286196.
STRINGi511145.b0620.

Structurei

3D structure databases

ProteinModelPortaliP0AEF4.
SMRiP0AEF4. Positions 7-132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 122117Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4106YYS. Bacteria.
COG4565. LUCA.
HOGENOMiHOG000034816.
InParanoidiP0AEF4.
KOiK07702.
OMAiLNHFTMN.
OrthoDBiEOG6T1WWP.
PhylomeDBiP0AEF4.

Family and domain databases

InterProiIPR011006. CheY-like_superfamily.
IPR024187. Sig_transdc_resp-reg_cit/mal.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR028141. Transcriptional_reg_dom.
[Graphical view]
PfamiPF12431. CitT. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFiPIRSF006171. RR_citrat_malat. 1 hit.
SMARTiSM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPLTLLIV EDETPLAEMH AEYIRHIPGF SQILLAGNLA QARMMIERFK
60 70 80 90 100
PGLILLDNYL PDGRGINLLH ELVQAHYPGD VVFTTAASDM ETVSEAVRCG
110 120 130 140 150
VFDYLIKPIA YERLGQTLTR FRQRKHMLES IDSASQKQID EMFNAYARGE
160 170 180 190 200
PKDELPTGID PLTLNAVRKL FKEPGVQHTA ETVAQALTIS RTTARRYLEY
210 220
CASRHLIIAE IVHGKVGRPQ RIYHSG
Length:226
Mass (Da):25,453
Last modified:December 6, 2005 - v1
Checksum:i2A47279EED145743
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46667 Genomic DNA. Translation: AAC28952.1.
U82598 Genomic DNA. Translation: AAB40820.1.
U00096 Genomic DNA. Translation: AAC73721.1.
AP009048 Genomic DNA. Translation: BAA35256.1.
PIRiB64796.
RefSeqiNP_415153.1. NC_000913.3.
WP_000126500.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73721; AAC73721; b0620.
BAA35256; BAA35256; BAA35256.
GeneIDi947008.
KEGGiecj:JW0612.
eco:b0620.
PATRICi32116420. VBIEscCol129921_0650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46667 Genomic DNA. Translation: AAC28952.1.
U82598 Genomic DNA. Translation: AAB40820.1.
U00096 Genomic DNA. Translation: AAC73721.1.
AP009048 Genomic DNA. Translation: BAA35256.1.
PIRiB64796.
RefSeqiNP_415153.1. NC_000913.3.
WP_000126500.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AEF4.
SMRiP0AEF4. Positions 7-132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263364. 6 interactions.
DIPiDIP-47831N.
IntActiP0AEF4. 15 interactions.
MINTiMINT-1286196.
STRINGi511145.b0620.

Proteomic databases

PaxDbiP0AEF4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73721; AAC73721; b0620.
BAA35256; BAA35256; BAA35256.
GeneIDi947008.
KEGGiecj:JW0612.
eco:b0620.
PATRICi32116420. VBIEscCol129921_0650.

Organism-specific databases

EchoBASEiEB3314.
EcoGeneiEG13544. dpiA.

Phylogenomic databases

eggNOGiENOG4106YYS. Bacteria.
COG4565. LUCA.
HOGENOMiHOG000034816.
InParanoidiP0AEF4.
KOiK07702.
OMAiLNHFTMN.
OrthoDBiEOG6T1WWP.
PhylomeDBiP0AEF4.

Enzyme and pathway databases

BioCyciEcoCyc:G6346-MONOMER.
ECOL316407:JW0612-MONOMER.

Miscellaneous databases

PROiP0AEF4.

Family and domain databases

InterProiIPR011006. CheY-like_superfamily.
IPR024187. Sig_transdc_resp-reg_cit/mal.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR028141. Transcriptional_reg_dom.
[Graphical view]
PfamiPF12431. CitT. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFiPIRSF006171. RR_citrat_malat. 1 hit.
SMARTiSM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Destabilized inheritance of pSC101 and other Escherichia coli plasmids by DpiA, a novel two-component system regulator."
    Ingmer H., Miller C.A., Cohen S.N.
    Mol. Microbiol. 29:49-59(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Anaerobic regulation of citrate fermentation by CitAB in Escherichia coli."
    Yamamoto K., Matsumoto F., Oshima T., Fujita N., Ogasawara N., Ishihama A.
    Biosci. Biotechnol. Biochem. 72:3011-3014(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Characterization of CitA-CitB signal transduction activating genes involved in anaerobic citrate catabolism in Escherichia coli."
    Yamamoto K., Matsumoto F., Minagawa S., Oshima T., Fujita N., Ogasawara N., Ishihama A.
    Biosci. Biotechnol. Biochem. 73:346-350(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, DNA-BINDING.

Entry informationi

Entry nameiDPIA_ECOLI
AccessioniPrimary (citable) accession number: P0AEF4
Secondary accession number(s): Q54149
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: January 20, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.