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Protein

Transcriptional regulatory protein DpiA

Gene

dpiA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox conditions. Regulates the transcription of citCDEFXGT, dpiAB, mdh and exuT. Binds specifically to the dpiB-citC intergenic region.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi180 – 199H-T-H motifBy similarityAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • DNA binding transcription factor activity Source: InterPro
  • phosphorelay response regulator activity Source: EcoCyc

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • regulation of transcription, DNA-templated Source: EcoCyc
  • response to external stimulus Source: InterPro
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation, Two-component regulatory system

Enzyme and pathway databases

BioCyciEcoCyc:G6346-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein DpiA
Alternative name(s):
Destabilizer of plasmid inheritance
Gene namesi
Name:dpiA
Synonyms:citB, criR, mpdA
Ordered Locus Names:b0620, JW0612
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13544 dpiA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000810681 – 226Transcriptional regulatory protein DpiAAdd BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei574-aspartylphosphatePROSITE-ProRule annotation1

Post-translational modificationi

Phosphorylated and activated by DpiB.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AEF4
PRIDEiP0AEF4

PTM databases

iPTMnetiP0AEF4

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4263364, 6 interactors
DIPiDIP-47831N
IntActiP0AEF4, 15 interactors
STRINGi316385.ECDH10B_0688

Structurei

3D structure databases

ProteinModelPortaliP0AEF4
SMRiP0AEF4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 122Response regulatoryPROSITE-ProRule annotationAdd BLAST117

Phylogenomic databases

eggNOGiENOG4106YYS Bacteria
COG4565 LUCA
HOGENOMiHOG000034816
InParanoidiP0AEF4
KOiK07702
OMAiALIDNFF
PhylomeDBiP0AEF4

Family and domain databases

CDDicd00156 REC, 1 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR024187 Sig_transdc_resp-reg_cit/mal
IPR001789 Sig_transdc_resp-reg_receiver
IPR028141 Transcriptional_reg_dom
PANTHERiPTHR45526 PTHR45526, 1 hit
PfamiView protein in Pfam
PF12431 CitT, 1 hit
PF00072 Response_reg, 1 hit
PIRSFiPIRSF006171 RR_citrat_malat, 1 hit
SMARTiView protein in SMART
SM00448 REC, 1 hit
SUPFAMiSSF52172 SSF52172, 1 hit
PROSITEiView protein in PROSITE
PS50110 RESPONSE_REGULATORY, 1 hit

Sequencei

Sequence statusi: Complete.

P0AEF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPLTLLIV EDETPLAEMH AEYIRHIPGF SQILLAGNLA QARMMIERFK
60 70 80 90 100
PGLILLDNYL PDGRGINLLH ELVQAHYPGD VVFTTAASDM ETVSEAVRCG
110 120 130 140 150
VFDYLIKPIA YERLGQTLTR FRQRKHMLES IDSASQKQID EMFNAYARGE
160 170 180 190 200
PKDELPTGID PLTLNAVRKL FKEPGVQHTA ETVAQALTIS RTTARRYLEY
210 220
CASRHLIIAE IVHGKVGRPQ RIYHSG
Length:226
Mass (Da):25,453
Last modified:December 6, 2005 - v1
Checksum:i2A47279EED145743
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46667 Genomic DNA Translation: AAC28952.1
U82598 Genomic DNA Translation: AAB40820.1
U00096 Genomic DNA Translation: AAC73721.1
AP009048 Genomic DNA Translation: BAA35256.1
PIRiB64796
RefSeqiNP_415153.1, NC_000913.3
WP_000126500.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73721; AAC73721; b0620
BAA35256; BAA35256; BAA35256
GeneIDi947008
KEGGiecj:JW0612
eco:b0620
PATRICifig|511145.12.peg.650

Similar proteinsi

Entry informationi

Entry nameiDPIA_ECOLI
AccessioniPrimary (citable) accession number: P0AEF4
Secondary accession number(s): Q54149
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health