P0AEF2 (DNAC_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA replication protein dnaC

Gene names

Name:	dnaC
Ordered Locus Names:	Z5961, ECs5321

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein is required for chromosomal replication. It forms, in concert with dnaB protein and other prepriming proteins dnaT, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N' By similarity.
Sequence similarities	Belongs to the dnaC family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Primosome
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	alpha DNA polymerase:primase complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	DNA replication protein dnaC		PRO_0000079955
Sites
	Site	69	1	Probably involved in the interaction with the dnaB protein By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AEF2 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: C5CC7232E21F11F1	FASTA	245	27,935
	10 20 30 40 50 60 MKNVGDLMQR LQKMMPAHIK PAFKTGEELL AWQKEQGAIR SAALERENRA MKMQRTFNRS 70 80 90 100 110 120 GIRPLHQNCS FENYRVECEG QMNALSKARQ YVEEFDGNIA SFIFSGKPGT GKNHLAAAIC 130 140 150 160 170 180 NELLLRGKSV LIITVADIMS AMKDTFRNSG TSEEQLLNDL SNVDLLVIDE IGVQTESKYE 190 200 210 220 230 240 KVIINQIVDR RSSSKRPTGM LTNSNMEEMT KLLGERVMDR MRLGNSLWVI FNWDSYRSRV TGKEY

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG59544.1. BA000007 Genomic DNA. Translation: BAB38744.1.
PIR	A91294. D86135.
RefSeq	NP_290977.1. NC_002655.2. NP_313348.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P0AEF2.
SMR	P0AEF2. Positions 64-237.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000026324; EBESCP00000025217; EBESCG00000025377. EBESCT00000059910; EBESCP00000057738; EBESCG00000058957.
GeneID	913577. 959692.
GenomeReviews	Gene locus Z5961 in contig AE005174_GR. Gene locus ECs5321 in contig BA000007_GR.
KEGG	ece:Z5961. ecs:ECs5321.
PATRIC	18360296. VBIEscCol44059_5279.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000009124.
HOGENOM	HBG575938.
OMA	VIFNWES.
ProtClustDB	PRK07952.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS5321-MONOMER.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR002611. IstB_ATP-bd. [Graphical view]
KO	K02315.
Pfam	PF01695. IstB_IS21. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAC_ECO57

Accession

Primary (citable) accession number: P0AEF2
Secondary accession number(s): P07905

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	December 6, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program