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P0AEF0

- DNAC_ECOLI

UniProt

P0AEF0 - DNAC_ECOLI

Protein

DNA replication protein DnaC

Gene

dnaC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    This protein is required for chromosomal replication. It forms, in concert with DnaB protein and other prepriming proteins DnaT, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N'.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei69 – 691Probably involved in the interaction with the DnaB protein

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. nucleoside-triphosphatase activity Source: InterPro
    3. protein binding Source: IntAct

    GO - Biological processi

    1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
    2. DNA strand elongation involved in DNA replication Source: EcoCyc

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10237-MONOMER.
    ECOL316407:JW4325-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA replication protein DnaC
    Gene namesi
    Name:dnaC
    Synonyms:dnaD
    Ordered Locus Names:b4361, JW4325
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10237. dnaC.

    Subcellular locationi

    GO - Cellular componenti

    1. primosome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Primosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 245245DNA replication protein DnaCPRO_0000079954Add
    BLAST

    Proteomic databases

    PaxDbiP0AEF0.
    PRIDEiP0AEF0.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AEF0.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    dnaBP0ACB09EBI-549012,EBI-548978

    Protein-protein interaction databases

    DIPiDIP-9457N.
    IntActiP0AEF0. 13 interactions.
    MINTiMINT-1276712.
    STRINGi511145.b4361.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AEF0.
    SMRiP0AEF0. Positions 53-232.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DnaC family.Curated

    Phylogenomic databases

    eggNOGiCOG1484.
    HOGENOMiHOG000120230.
    KOiK02315.
    OMAiVIFNWES.
    OrthoDBiEOG6TR0CP.
    PhylomeDBiP0AEF0.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR028350. DNAC/ATP-binding.
    IPR002611. IstB_ATP-bd.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF01695. IstB_IS21. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003073. DNAC_TnpB_IstB. 1 hit.
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0AEF0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNVGDLMQR LQKMMPAHIK PAFKTGEELL AWQKEQGAIR SAALERENRA    50
    MKMQRTFNRS GIRPLHQNCS FENYRVECEG QMNALSKARQ YVEEFDGNIA 100
    SFIFSGKPGT GKNHLAAAIC NELLLRGKSV LIITVADIMS AMKDTFRNSG 150
    TSEEQLLNDL SNVDLLVIDE IGVQTESKYE KVIINQIVDR RSSSKRPTGM 200
    LTNSNMEEMT KLLGERVMDR MRLGNSLWVI FNWDSYRSRV TGKEY 245
    Length:245
    Mass (Da):27,935
    Last modified:December 6, 2005 - v1
    Checksum:iC5CC7232E21F11F1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61D → A(PubMed:3301836)Curated
    Sequence conflicti6 – 61D → A(PubMed:3006041)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04030 Genomic DNA. Translation: AAA23700.1.
    U14003 Genomic DNA. Translation: AAA97260.1.
    U00096 Genomic DNA. Translation: AAC77317.1.
    AP009048 Genomic DNA. Translation: BAE78351.1.
    PIRiA28484. XMECNC.
    RefSeqiNP_418781.1. NC_000913.3.
    YP_492492.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77317; AAC77317; b4361.
    BAE78351; BAE78351; BAE78351.
    GeneIDi12933761.
    948864.
    KEGGiecj:Y75_p4246.
    eco:b4361.
    PATRICi32124330. VBIEscCol129921_4508.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04030 Genomic DNA. Translation: AAA23700.1 .
    U14003 Genomic DNA. Translation: AAA97260.1 .
    U00096 Genomic DNA. Translation: AAC77317.1 .
    AP009048 Genomic DNA. Translation: BAE78351.1 .
    PIRi A28484. XMECNC.
    RefSeqi NP_418781.1. NC_000913.3.
    YP_492492.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AEF0.
    SMRi P0AEF0. Positions 53-232.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9457N.
    IntActi P0AEF0. 13 interactions.
    MINTi MINT-1276712.
    STRINGi 511145.b4361.

    Proteomic databases

    PaxDbi P0AEF0.
    PRIDEi P0AEF0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC77317 ; AAC77317 ; b4361 .
    BAE78351 ; BAE78351 ; BAE78351 .
    GeneIDi 12933761.
    948864.
    KEGGi ecj:Y75_p4246.
    eco:b4361.
    PATRICi 32124330. VBIEscCol129921_4508.

    Organism-specific databases

    EchoBASEi EB0233.
    EcoGenei EG10237. dnaC.

    Phylogenomic databases

    eggNOGi COG1484.
    HOGENOMi HOG000120230.
    KOi K02315.
    OMAi VIFNWES.
    OrthoDBi EOG6TR0CP.
    PhylomeDBi P0AEF0.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10237-MONOMER.
    ECOL316407:JW4325-MONOMER.

    Miscellaneous databases

    PROi P0AEF0.

    Gene expression databases

    Genevestigatori P0AEF0.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR028350. DNAC/ATP-binding.
    IPR002611. IstB_ATP-bd.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF01695. IstB_IS21. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003073. DNAC_TnpB_IstB. 1 hit.
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structure of Escherichia coli dnaC. Identification of a cysteine residue possibly involved in association with dnaB protein."
      Nakayama N., Bond M.W., Miyajima A., Kobori J., Arai K.
      J. Biol. Chem. 262:10475-10480(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Operon structure of dnaT and dnaC genes essential for normal and stable DNA replication of Escherichia coli chromosome."
      Masai H., Arai K.
      J. Biol. Chem. 263:15083-15093(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
      Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Cloning of the Escherichia coli gene for primosomal protein I: the relationship to dnaT, essential for chromosomal DNA replication."
      Masai H., Bond M.W., Arai K.
      Proc. Natl. Acad. Sci. U.S.A. 83:1256-1260(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
      Strain: K12.
    7. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.

    Entry informationi

    Entry nameiDNAC_ECOLI
    AccessioniPrimary (citable) accession number: P0AEF0
    Secondary accession number(s): P07905, Q2M5V5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3