Reviewed, UniProtKB/Swiss-Prot P0AEF0 (DNAC_ECOLI)

Names and origin

Protein names

Recommended name:
    DNA replication protein dnaC

Gene names

Name:	dnaC
Synonyms:	dnaD
Ordered Locus Names:	b4361, JW4325

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein is required for chromosomal replication. It forms, in concert with dnaB protein and other prepriming proteins dnaT, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N'.
Sequence similarities	Belongs to the dnaC family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Primosome
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	alpha DNA polymerase:primase complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
tsf	P0A6P1	1	EBI-549012,EBI-301164

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	DNA replication protein dnaC		PRO_0000079954
Sites
	Site	69	1	Probably involved in the interaction with the dnaB protein
Experimental info
	Sequence conflict	6	1	D → A Ref.1
	Sequence conflict	6	1	D → A Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P0AEF0-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: C5CC7232E21F11F1	FASTA	245	27,935
	10 20 30 40 50 60 MKNVGDLMQR LQKMMPAHIK PAFKTGEELL AWQKEQGAIR SAALERENRA MKMQRTFNRS 70 80 90 100 110 120 GIRPLHQNCS FENYRVECEG QMNALSKARQ YVEEFDGNIA SFIFSGKPGT GKNHLAAAIC 130 140 150 160 170 180 NELLLRGKSV LIITVADIMS AMKDTFRNSG TSEEQLLNDL SNVDLLVIDE IGVQTESKYE 190 200 210 220 230 240 KVIINQIVDR RSSSKRPTGM LTNSNMEEMT KLLGERVMDR MRLGNSLWVI FNWDSYRSRV TGKEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of Escherichia coli dnaC. Identification of a cysteine residue possibly involved in association with dnaB protein." Nakayama N., Bond M.W., Miyajima A., Kobori J., Arai K. J. Biol. Chem. 262:10475-10480(1987) [PubMed: 3301836] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Operon structure of dnaT and dnaC genes essential for normal and stable DNA replication of Escherichia coli chromosome." Masai H., Arai K. J. Biol. Chem. 263:15083-15093(1988) [PubMed: 2844800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Cloning of the Escherichia coli gene for primosomal protein I: the relationship to dnaT, essential for chromosomal DNA replication." Masai H., Bond M.W., Arai K. Proc. Natl. Acad. Sci. U.S.A. 83:1256-1260(1986) [PubMed: 3006041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14. Strain: K12.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04030 Genomic DNA. Translation: AAA23700.1. U14003 Genomic DNA. Translation: AAA97260.1. U00096 Genomic DNA. Translation: AAC77317.1. AP009048 Genomic DNA. Translation: BAE78351.1.
PIR	XMECNC. A28484.
RefSeq	AP_004850.1. NP_418781.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AEF0. 12 interactions.
STRING	P0AEF0.
2-D gel databases
ECO2DBASE	I030.3. 6TH EDITION.
Genome annotation databases
GeneID	948864.
GenomeReviews	Gene locus JW4325 in contig AP009048_GR. Gene locus b4361 in contig U00096_GR.
KEGG	ecj:JW4325. eco:b4361.
Organism-specific databases
EchoBASE	EB0233.
EcoGene	EG10237. dnaC.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AEF0.
OMA	VIFNWES
Enzyme and pathway databases
BioCyc	EcoCyc:EG10237-MON. ECOL168927:B4361-MON.
Gene expression databases
Genevestigator	P0AEF0.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAC_ECOLI

Accession

Primary (citable) accession number: P0AEF0
Secondary accession number(s): P07905, Q2M5V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	December 6, 2005
Last modified:	November 24, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)