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Protein

DNA replication protein DnaC

Gene

dnaC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is required for chromosomal replication. It forms, in concert with DnaB protein and other prepriming proteins DnaT, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N'.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei69 – 691Probably involved in the interaction with the DnaB protein

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: GO_Central
  • DNA replication, synthesis of RNA primer Source: UniProtKB-KW
  • DNA strand elongation involved in DNA replication Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10237-MONOMER.
ECOL316407:JW4325-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication protein DnaC
Gene namesi
Name:dnaC
Synonyms:dnaD
Ordered Locus Names:b4361, JW4325
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10237. dnaC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245DNA replication protein DnaCPRO_0000079954Add
BLAST

Proteomic databases

PaxDbiP0AEF0.
PRIDEiP0AEF0.

Expressioni

Inductioni

Induced by hydroxyurea.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaBP0ACB09EBI-549012,EBI-548978

Protein-protein interaction databases

DIPiDIP-9457N.
IntActiP0AEF0. 13 interactions.
MINTiMINT-1276712.
STRINGi511145.b4361.

Structurei

3D structure databases

ProteinModelPortaliP0AEF0.
SMRiP0AEF0. Positions 53-232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DnaC family.Curated

Phylogenomic databases

eggNOGiCOG1484.
HOGENOMiHOG000120230.
InParanoidiP0AEF0.
KOiK02315.
OMAiVGDLMKR.
OrthoDBiEOG6TR0CP.
PhylomeDBiP0AEF0.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR028350. DNAC/ATP-binding.
IPR002611. IstB_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01695. IstB_IS21. 1 hit.
[Graphical view]
PIRSFiPIRSF003073. DNAC_TnpB_IstB. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AEF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNVGDLMQR LQKMMPAHIK PAFKTGEELL AWQKEQGAIR SAALERENRA
60 70 80 90 100
MKMQRTFNRS GIRPLHQNCS FENYRVECEG QMNALSKARQ YVEEFDGNIA
110 120 130 140 150
SFIFSGKPGT GKNHLAAAIC NELLLRGKSV LIITVADIMS AMKDTFRNSG
160 170 180 190 200
TSEEQLLNDL SNVDLLVIDE IGVQTESKYE KVIINQIVDR RSSSKRPTGM
210 220 230 240
LTNSNMEEMT KLLGERVMDR MRLGNSLWVI FNWDSYRSRV TGKEY
Length:245
Mass (Da):27,935
Last modified:December 6, 2005 - v1
Checksum:iC5CC7232E21F11F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04030 Genomic DNA. Translation: AAA23700.1.
U14003 Genomic DNA. Translation: AAA97260.1.
U00096 Genomic DNA. Translation: AAC77317.1.
AP009048 Genomic DNA. Translation: BAE78351.1.
PIRiA28484. XMECNC.
RefSeqiNP_418781.1. NC_000913.3.
WP_000799911.1. NZ_CP010445.1.

Genome annotation databases

EnsemblBacteriaiAAC77317; AAC77317; b4361.
BAE78351; BAE78351; BAE78351.
GeneIDi948864.
KEGGieco:b4361.
PATRICi32124330. VBIEscCol129921_4508.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04030 Genomic DNA. Translation: AAA23700.1.
U14003 Genomic DNA. Translation: AAA97260.1.
U00096 Genomic DNA. Translation: AAC77317.1.
AP009048 Genomic DNA. Translation: BAE78351.1.
PIRiA28484. XMECNC.
RefSeqiNP_418781.1. NC_000913.3.
WP_000799911.1. NZ_CP010445.1.

3D structure databases

ProteinModelPortaliP0AEF0.
SMRiP0AEF0. Positions 53-232.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-9457N.
IntActiP0AEF0. 13 interactions.
MINTiMINT-1276712.
STRINGi511145.b4361.

Proteomic databases

PaxDbiP0AEF0.
PRIDEiP0AEF0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77317; AAC77317; b4361.
BAE78351; BAE78351; BAE78351.
GeneIDi948864.
KEGGieco:b4361.
PATRICi32124330. VBIEscCol129921_4508.

Organism-specific databases

EchoBASEiEB0233.
EcoGeneiEG10237. dnaC.

Phylogenomic databases

eggNOGiCOG1484.
HOGENOMiHOG000120230.
InParanoidiP0AEF0.
KOiK02315.
OMAiVGDLMKR.
OrthoDBiEOG6TR0CP.
PhylomeDBiP0AEF0.

Enzyme and pathway databases

BioCyciEcoCyc:EG10237-MONOMER.
ECOL316407:JW4325-MONOMER.

Miscellaneous databases

PROiP0AEF0.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR028350. DNAC/ATP-binding.
IPR002611. IstB_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01695. IstB_IS21. 1 hit.
[Graphical view]
PIRSFiPIRSF003073. DNAC_TnpB_IstB. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of Escherichia coli dnaC. Identification of a cysteine residue possibly involved in association with dnaB protein."
    Nakayama N., Bond M.W., Miyajima A., Kobori J., Arai K.
    J. Biol. Chem. 262:10475-10480(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Operon structure of dnaT and dnaC genes essential for normal and stable DNA replication of Escherichia coli chromosome."
    Masai H., Arai K.
    J. Biol. Chem. 263:15083-15093(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Cloning of the Escherichia coli gene for primosomal protein I: the relationship to dnaT, essential for chromosomal DNA replication."
    Masai H., Bond M.W., Arai K.
    Proc. Natl. Acad. Sci. U.S.A. 83:1256-1260(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
    Strain: K12.
  7. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  8. "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia coli."
    Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J., Walker G.C.
    Mol. Cell 36:845-860(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY HYDROXYUREA.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Entry informationi

Entry nameiDNAC_ECOLI
AccessioniPrimary (citable) accession number: P0AEF0
Secondary accession number(s): P07905, Q2M5V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: December 6, 2005
Last modified: July 22, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.