P0AEF0 (DNAC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA replication protein DnaC

Gene names

Name:	dnaC
Synonyms:	dnaD
Ordered Locus Names:	b4361, JW4325

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is required for chromosomal replication. It forms, in concert with DnaB protein and other prepriming proteins DnaT, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N'.
Sequence similarities	Belongs to the DnaC family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Primosome
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW DNA strand elongation involved in DNA replication Inferred from genetic interaction PubMed 6277866. Source: EcoCyc
Cellular_component	replisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
dnaB	P0ACB0	2	EBI-549012,EBI-548978

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	DNA replication protein DnaC		PRO_0000079954
Sites
	Site	69	1	Probably involved in the interaction with the DnaB protein
Experimental info
	Sequence conflict	6	1	D → A Ref.1
	Sequence conflict	6	1	D → A Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P0AEF0 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: C5CC7232E21F11F1	FASTA	245	27,935
	10 20 30 40 50 60 MKNVGDLMQR LQKMMPAHIK PAFKTGEELL AWQKEQGAIR SAALERENRA MKMQRTFNRS 70 80 90 100 110 120 GIRPLHQNCS FENYRVECEG QMNALSKARQ YVEEFDGNIA SFIFSGKPGT GKNHLAAAIC 130 140 150 160 170 180 NELLLRGKSV LIITVADIMS AMKDTFRNSG TSEEQLLNDL SNVDLLVIDE IGVQTESKYE 190 200 210 220 230 240 KVIINQIVDR RSSSKRPTGM LTNSNMEEMT KLLGERVMDR MRLGNSLWVI FNWDSYRSRV TGKEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of Escherichia coli dnaC. Identification of a cysteine residue possibly involved in association with dnaB protein." Nakayama N., Bond M.W., Miyajima A., Kobori J., Arai K. J. Biol. Chem. 262:10475-10480(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Operon structure of dnaT and dnaC genes essential for normal and stable DNA replication of Escherichia coli chromosome." Masai H., Arai K. J. Biol. Chem. 263:15083-15093(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Cloning of the Escherichia coli gene for primosomal protein I: the relationship to dnaT, essential for chromosomal DNA replication." Masai H., Bond M.W., Arai K. Proc. Natl. Acad. Sci. U.S.A. 83:1256-1260(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14. Strain: K12.
[7]	"Escherichia coli proteome analysis using the gene-protein database." VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C. Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY 2D-GEL.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04030 Genomic DNA. Translation: AAA23700.1. U14003 Genomic DNA. Translation: AAA97260.1. U00096 Genomic DNA. Translation: AAC77317.1. AP009048 Genomic DNA. Translation: BAE78351.1.
PIR	XMECNC. A28484.
RefSeq	NP_418781.1. NC_000913.2. YP_492492.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AEF0.
SMR	P0AEF0. Positions 79-222.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9457N.
IntAct	P0AEF0. 13 interactions.
MINT	MINT-1276712.
STRING	511145.b4361.
Proteomic databases
PaxDb	P0AEF0.
PRIDE	P0AEF0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77317; AAC77317; b4361. BAE78351; BAE78351; BAE78351.
GeneID	12933761. 948864.
KEGG	ecj:Y75_p4246. eco:b4361.
PATRIC	32124330. VBIEscCol129921_4508.
Organism-specific databases
EchoBASE	EB0233.
EcoGene	EG10237. dnaC.
Phylogenomic databases
eggNOG	COG1484.
HOGENOM	HOG000120230.
KO	K02315.
OMA	VGDLMKR.
ProtClustDB	PRK07952.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10237-MONOMER. ECOL316407:JW4325-MONOMER.
Gene expression databases
Genevestigator	P0AEF0.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR002611. IstB_ATP-bd. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF01695. IstB_IS21. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAC_ECOLI

Accession

Primary (citable) accession number: P0AEF0
Secondary accession number(s): P07905, Q2M5V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	December 6, 2005
Last modified:	May 29, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program