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Protein

DNA adenine methylase

Gene

dam

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.By similarity

Catalytic activityi

S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10S-adenosyl-L-methionineBy similarity1
Binding sitei14S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei54S-adenosyl-L-methionineBy similarity1
Binding sitei181S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processDNA replication
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.72 2026

Protein family/group databases

REBASEi5592 M.EcoKO157DamP

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
Gene namesi
Name:dam
Ordered Locus Names:Z4740, ECs4229
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879921 – 278DNA adenine methylaseAdd BLAST278

Interactioni

Protein-protein interaction databases

STRINGi155864.Z4740

Structurei

3D structure databases

ProteinModelPortaliP0AEE9
SMRiP0AEE9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiENOG4105DFE Bacteria
COG0338 LUCA
HOGENOMiHOG000281348
KOiK06223
OMAiMNRHGFN

Family and domain databases

Gene3Di1.10.1020.10, 1 hit
InterProiView protein in InterPro
IPR023095 Ade_MeTrfase_dom_2
IPR002052 DNA_methylase_N6_adenine_CS
IPR012263 M_m6A_EcoRV
IPR012327 MeTrfase_D12
IPR029063 SAM-dependent_MTases
PANTHERiPTHR30481 PTHR30481, 1 hit
PfamiView protein in Pfam
PF02086 MethyltransfD12, 1 hit
PIRSFiPIRSF000398 M_m6A_EcoRV, 1 hit
PRINTSiPR00505 D12N6MTFRASE
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00571 dam, 1 hit
PROSITEiView protein in PROSITE
PS00092 N6_MTASE, 1 hit

Sequencei

Sequence statusi: Complete.

P0AEE9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY
60 70 80 90 100
ILADINSDLI SLYNIVKMRT DEYVQAAREL FVPETNCAEV YYQFREEFNK
110 120 130 140 150
SQDPFRRAVL FLYLNRYGYN GLCRYNLRGE FNVPFGRYKK PYFPEAELYH
160 170 180 190 200
FAEKAQNAFF YCESYADSMA RADDASVVYC DPPYAPLSAT ANFTAYHTNS
210 220 230 240 250
FTLEQQAHLA EIAEGLVERH IPVLISNHDT MLTREWYQRA KLHVVKVRRS
260 270
ISSNGGTRKK VDELLALYKP GVVSPAKK
Length:278
Mass (Da):32,100
Last modified:July 21, 1986 - v1
Checksum:iB2FA5D1FDC863CB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG58487.1
BA000007 Genomic DNA Translation: BAB37652.1
PIRiC86003
E91157
RefSeqiNP_312256.1, NC_002695.1
WP_000742143.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG58487; AAG58487; Z4740
BAB37652; BAB37652; BAB37652
GeneIDi915915
KEGGiece:Z4740
ecs:ECs4229
PATRICifig|386585.9.peg.4415

Entry informationi

Entry nameiDMA_ECO57
AccessioniPrimary (citable) accession number: P0AEE9
Secondary accession number(s): P00475
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 25, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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