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P0AEE9

- DMA_ECO57

UniProt

P0AEE9 - DMA_ECO57

Protein

DNA adenine methylase

Gene

dam

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication By similarity.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101S-adenosyl-L-methionineBy similarity
    Binding sitei14 – 141S-adenosyl-L-methionine; via amide nitrogenBy similarity
    Binding sitei54 – 541S-adenosyl-L-methionineBy similarity
    Binding sitei181 – 1811S-adenosyl-L-methionineBy similarity

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-4199-MONOMER.
    ECOO157:DAM-MONOMER.

    Protein family/group databases

    REBASEi5592. M.EcoKO157DamP.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA adenine methylase (EC:2.1.1.72)
    Alternative name(s):
    DNA adenine methyltransferase
    Deoxyadenosyl-methyltransferase
    Gene namesi
    Name:dam
    Ordered Locus Names:Z4740, ECs4229
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 278278DNA adenine methylasePRO_0000087992Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-1230135.
    STRINGi155864.Z4740.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AEE9.
    SMRiP0AEE9. Positions 3-270.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0338.
    HOGENOMiHOG000281348.
    KOiK06223.
    OMAiKFTREIY.
    OrthoDBiEOG6CS08V.

    Family and domain databases

    Gene3Di1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProiIPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00571. dam. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AEE9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY    50
    ILADINSDLI SLYNIVKMRT DEYVQAAREL FVPETNCAEV YYQFREEFNK 100
    SQDPFRRAVL FLYLNRYGYN GLCRYNLRGE FNVPFGRYKK PYFPEAELYH 150
    FAEKAQNAFF YCESYADSMA RADDASVVYC DPPYAPLSAT ANFTAYHTNS 200
    FTLEQQAHLA EIAEGLVERH IPVLISNHDT MLTREWYQRA KLHVVKVRRS 250
    ISSNGGTRKK VDELLALYKP GVVSPAKK 278
    Length:278
    Mass (Da):32,100
    Last modified:July 21, 1986 - v1
    Checksum:iB2FA5D1FDC863CB9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG58487.1.
    BA000007 Genomic DNA. Translation: BAB37652.1.
    PIRiC86003.
    E91157.
    RefSeqiNP_289926.1. NC_002655.2.
    NP_312256.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG58487; AAG58487; Z4740.
    BAB37652; BAB37652; BAB37652.
    GeneIDi915915.
    958843.
    KEGGiece:Z4740.
    ecs:ECs4229.
    PATRICi18358011. VBIEscCol44059_4172.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG58487.1 .
    BA000007 Genomic DNA. Translation: BAB37652.1 .
    PIRi C86003.
    E91157.
    RefSeqi NP_289926.1. NC_002655.2.
    NP_312256.1. NC_002695.1.

    3D structure databases

    ProteinModelPortali P0AEE9.
    SMRi P0AEE9. Positions 3-270.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1230135.
    STRINGi 155864.Z4740.

    Protein family/group databases

    REBASEi 5592. M.EcoKO157DamP.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG58487 ; AAG58487 ; Z4740 .
    BAB37652 ; BAB37652 ; BAB37652 .
    GeneIDi 915915.
    958843.
    KEGGi ece:Z4740.
    ecs:ECs4229.
    PATRICi 18358011. VBIEscCol44059_4172.

    Phylogenomic databases

    eggNOGi COG0338.
    HOGENOMi HOG000281348.
    KOi K06223.
    OMAi KFTREIY.
    OrthoDBi EOG6CS08V.

    Enzyme and pathway databases

    BioCyci ECOL386585:GJFA-4199-MONOMER.
    ECOO157:DAM-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProi IPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00571. dam. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiDMA_ECO57
    AccessioniPrimary (citable) accession number: P0AEE9
    Secondary accession number(s): P00475
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3