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P0AEE9

- DMA_ECO57

UniProt

P0AEE9 - DMA_ECO57

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Protein

DNA adenine methylase

Gene

dam

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101S-adenosyl-L-methionineBy similarity
Binding sitei14 – 141S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei54 – 541S-adenosyl-L-methionineBy similarity
Binding sitei181 – 1811S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciECOL386585:GJFA-4199-MONOMER.
ECOO157:DAM-MONOMER.

Protein family/group databases

REBASEi5592. M.EcoKO157DamP.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
Gene namesi
Name:dam
Ordered Locus Names:Z4740, ECs4229
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278DNA adenine methylasePRO_0000087992Add
BLAST

Interactioni

Protein-protein interaction databases

MINTiMINT-1230135.
STRINGi155864.Z4740.

Structurei

3D structure databases

ProteinModelPortaliP0AEE9.
SMRiP0AEE9. Positions 3-270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0338.
HOGENOMiHOG000281348.
KOiK06223.
OMAiKFTREIY.
OrthoDBiEOG6CS08V.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEE9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY
60 70 80 90 100
ILADINSDLI SLYNIVKMRT DEYVQAAREL FVPETNCAEV YYQFREEFNK
110 120 130 140 150
SQDPFRRAVL FLYLNRYGYN GLCRYNLRGE FNVPFGRYKK PYFPEAELYH
160 170 180 190 200
FAEKAQNAFF YCESYADSMA RADDASVVYC DPPYAPLSAT ANFTAYHTNS
210 220 230 240 250
FTLEQQAHLA EIAEGLVERH IPVLISNHDT MLTREWYQRA KLHVVKVRRS
260 270
ISSNGGTRKK VDELLALYKP GVVSPAKK
Length:278
Mass (Da):32,100
Last modified:July 21, 1986 - v1
Checksum:iB2FA5D1FDC863CB9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG58487.1.
BA000007 Genomic DNA. Translation: BAB37652.1.
PIRiC86003.
E91157.
RefSeqiNP_289926.1. NC_002655.2.
NP_312256.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG58487; AAG58487; Z4740.
BAB37652; BAB37652; BAB37652.
GeneIDi915915.
958843.
KEGGiece:Z4740.
ecs:ECs4229.
PATRICi18358011. VBIEscCol44059_4172.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG58487.1 .
BA000007 Genomic DNA. Translation: BAB37652.1 .
PIRi C86003.
E91157.
RefSeqi NP_289926.1. NC_002655.2.
NP_312256.1. NC_002695.1.

3D structure databases

ProteinModelPortali P0AEE9.
SMRi P0AEE9. Positions 3-270.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1230135.
STRINGi 155864.Z4740.

Protein family/group databases

REBASEi 5592. M.EcoKO157DamP.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG58487 ; AAG58487 ; Z4740 .
BAB37652 ; BAB37652 ; BAB37652 .
GeneIDi 915915.
958843.
KEGGi ece:Z4740.
ecs:ECs4229.
PATRICi 18358011. VBIEscCol44059_4172.

Phylogenomic databases

eggNOGi COG0338.
HOGENOMi HOG000281348.
KOi K06223.
OMAi KFTREIY.
OrthoDBi EOG6CS08V.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-4199-MONOMER.
ECOO157:DAM-MONOMER.

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProi IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 1 hit.
[Graphical view ]
PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiDMA_ECO57
AccessioniPrimary (citable) accession number: P0AEE9
Secondary accession number(s): P00475
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 1, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3