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Protein

DNA adenine methylase

Gene

dam

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.

Catalytic activityi

S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10S-adenosyl-L-methionineBy similarity1
Binding sitei14S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei54S-adenosyl-L-methionineBy similarity1
Binding sitei181S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

  • S-adenosyl-L-methionine binding Source: EcoCyc
  • sequence-specific DNA binding Source: EcoCyc
  • site-specific DNA-methyltransferase (adenine-specific) activity Source: EcoCyc

GO - Biological processi

  • bacterial-type DNA replication initiation Source: EcoCyc
  • DNA-dependent DNA replication Source: EcoliWiki
  • DNA methylation on adenine Source: EcoCyc
  • mismatch repair Source: EcoliWiki
  • response to UV Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processDNA replication
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG10204-MONOMER
MetaCyc:EG10204-MONOMER
BRENDAi2.1.1.72 2026

Protein family/group databases

REBASEi2396 M.EcoKDam

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.EcoDam
Gene namesi
Name:dam
Ordered Locus Names:b3387, JW3350
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10204 dam

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075075

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879911 – 278DNA adenine methylaseAdd BLAST278

Proteomic databases

PaxDbiP0AEE8
PRIDEiP0AEE8

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
glpDP130353EBI-548491,EBI-548509

Protein-protein interaction databases

BioGridi4261860, 125 interactors
DIPiDIP-47948N
IntActiP0AEE8, 24 interactors
STRINGi316385.ECDH10B_3562

Structurei

Secondary structure

1278
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni11 – 13Combined sources3
Helixi15 – 17Combined sources3
Helixi18 – 24Combined sources7
Beta strandi29 – 33Combined sources5
Helixi40 – 43Combined sources4
Beta strandi48 – 55Combined sources8
Helixi57 – 68Combined sources12
Helixi70 – 78Combined sources9
Helixi79 – 81Combined sources3
Helixi83 – 85Combined sources3
Helixi88 – 100Combined sources13
Helixi104 – 117Combined sources14
Helixi119 – 121Combined sources3
Helixi145 – 154Combined sources10
Helixi155 – 157Combined sources3
Beta strandi158 – 162Combined sources5
Helixi165 – 169Combined sources5
Beta strandi176 – 180Combined sources5
Helixi203 – 218Combined sources16
Beta strandi223 – 228Combined sources6
Helixi231 – 236Combined sources6
Turni237 – 239Combined sources3
Beta strandi240 – 245Combined sources6
Beta strandi263 – 268Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G1PX-ray1.89A/B1-278[»]
2OREX-ray2.99D/E/F1-278[»]
4GBEX-ray2.66D/E/F1-278[»]
4GOLX-ray2.57D/E/F1-278[»]
4GOMX-ray2.45D/E/F1-278[»]
4GONX-ray2.72D/E/F1-278[»]
4GOOX-ray2.70D/E/F1-278[»]
4RTJX-ray1.99A1-278[»]
4RTKX-ray1.96A1-278[»]
4RTLX-ray2.19A1-278[»]
4RTMX-ray2.50A1-278[»]
4RTNX-ray2.59A1-278[»]
4RTOX-ray2.69A1-278[»]
4RTPX-ray2.39A1-278[»]
4RTQX-ray2.00A1-278[»]
4RTRX-ray2.39A1-278[»]
4RTSX-ray2.49A1-278[»]
ProteinModelPortaliP0AEE8
SMRiP0AEE8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEE8

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiENOG4105DFE Bacteria
COG0338 LUCA
HOGENOMiHOG000281348
InParanoidiP0AEE8
KOiK06223
OMAiMNRHGFN
PhylomeDBiP0AEE8

Family and domain databases

Gene3Di1.10.1020.10, 1 hit
InterProiView protein in InterPro
IPR023095 Ade_MeTrfase_dom_2
IPR002052 DNA_methylase_N6_adenine_CS
IPR012263 M_m6A_EcoRV
IPR012327 MeTrfase_D12
IPR029063 SAM-dependent_MTases
PANTHERiPTHR30481 PTHR30481, 1 hit
PfamiView protein in Pfam
PF02086 MethyltransfD12, 1 hit
PIRSFiPIRSF000398 M_m6A_EcoRV, 1 hit
PRINTSiPR00505 D12N6MTFRASE
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00571 dam, 1 hit
PROSITEiView protein in PROSITE
PS00092 N6_MTASE, 1 hit

Sequencei

Sequence statusi: Complete.

P0AEE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY
60 70 80 90 100
ILADINSDLI SLYNIVKMRT DEYVQAAREL FVPETNCAEV YYQFREEFNK
110 120 130 140 150
SQDPFRRAVL FLYLNRYGYN GLCRYNLRGE FNVPFGRYKK PYFPEAELYH
160 170 180 190 200
FAEKAQNAFF YCESYADSMA RADDASVVYC DPPYAPLSAT ANFTAYHTNS
210 220 230 240 250
FTLEQQAHLA EIAEGLVERH IPVLISNHDT MLTREWYQRA KLHVVKVRRS
260 270
ISSNGGTRKK VDELLALYKP GVVSPAKK
Length:278
Mass (Da):32,100
Last modified:July 21, 1986 - v1
Checksum:iB2FA5D1FDC863CB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01600 Genomic DNA Translation: AAA23664.1
V00272 Genomic DNA Translation: CAA23530.1
Z19601 Genomic DNA Translation: CAA79668.1
U18997 Genomic DNA Translation: AAA58184.1
U00096 Genomic DNA Translation: AAC76412.1
AP009048 Genomic DNA Translation: BAE77904.1
X15162 Genomic DNA Translation: CAA33254.1
PIRiA00555 XYECDA
RefSeqiNP_417846.1, NC_000913.3
WP_000742143.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76412; AAC76412; b3387
BAE77904; BAE77904; BAE77904
GeneIDi947893
KEGGiecj:JW3350
eco:b3387
PATRICifig|1411691.4.peg.3343

Similar proteinsi

Entry informationi

Entry nameiDMA_ECOLI
AccessioniPrimary (citable) accession number: P0AEE8
Secondary accession number(s): P00475, Q2M752
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: March 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health