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Protein

DNA adenine methylase

Gene

dam

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101S-adenosyl-L-methionineBy similarity
Binding sitei14 – 141S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei54 – 541S-adenosyl-L-methionineBy similarity
Binding sitei181 – 1811S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

  1. DNA binding Source: EcoCyc
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: EcoliWiki

GO - Biological processi

  1. DNA-dependent DNA replication Source: EcoliWiki
  2. DNA methylation Source: EcoCyc
  3. mismatch repair Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG10204-MONOMER.
ECOL316407:JW3350-MONOMER.

Protein family/group databases

REBASEi2396. M.EcoKDam.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.EcoDam
Gene namesi
Name:dam
Ordered Locus Names:b3387, JW3350
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10204. dam.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278DNA adenine methylasePRO_0000087991Add
BLAST

Proteomic databases

PaxDbiP0AEE8.
PRIDEiP0AEE8.

Expressioni

Gene expression databases

GenevestigatoriP0AEE8.

Interactioni

Protein-protein interaction databases

DIPiDIP-47948N.
IntActiP0AEE8. 24 interactions.
MINTiMINT-1230150.
STRINGi511145.b3387.

Structurei

Secondary structure

1
278
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni11 – 133Combined sources
Helixi15 – 173Combined sources
Helixi18 – 247Combined sources
Beta strandi29 – 335Combined sources
Helixi40 – 434Combined sources
Beta strandi48 – 558Combined sources
Helixi57 – 6812Combined sources
Helixi70 – 789Combined sources
Helixi79 – 813Combined sources
Helixi83 – 853Combined sources
Helixi88 – 10013Combined sources
Helixi104 – 11714Combined sources
Helixi119 – 1213Combined sources
Helixi145 – 15410Combined sources
Helixi155 – 1573Combined sources
Beta strandi158 – 1625Combined sources
Helixi165 – 1695Combined sources
Beta strandi176 – 1805Combined sources
Helixi203 – 21816Combined sources
Beta strandi223 – 2286Combined sources
Helixi231 – 2366Combined sources
Turni237 – 2393Combined sources
Beta strandi240 – 2456Combined sources
Beta strandi263 – 2686Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2G1PX-ray1.89A/B1-278[»]
2OREX-ray2.99D/E/F1-278[»]
4GBEX-ray2.66D/E/F1-278[»]
4GOLX-ray2.57D/E/F1-278[»]
4GOMX-ray2.45D/E/F1-278[»]
4GONX-ray2.72D/E/F1-278[»]
4GOOX-ray2.70D/E/F1-278[»]
ProteinModelPortaliP0AEE8.
SMRiP0AEE8. Positions 3-270.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEE8.

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0338.
HOGENOMiHOG000281348.
InParanoidiP0AEE8.
KOiK06223.
OMAiMNRHGFN.
OrthoDBiEOG6CS08V.
PhylomeDBiP0AEE8.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY
60 70 80 90 100
ILADINSDLI SLYNIVKMRT DEYVQAAREL FVPETNCAEV YYQFREEFNK
110 120 130 140 150
SQDPFRRAVL FLYLNRYGYN GLCRYNLRGE FNVPFGRYKK PYFPEAELYH
160 170 180 190 200
FAEKAQNAFF YCESYADSMA RADDASVVYC DPPYAPLSAT ANFTAYHTNS
210 220 230 240 250
FTLEQQAHLA EIAEGLVERH IPVLISNHDT MLTREWYQRA KLHVVKVRRS
260 270
ISSNGGTRKK VDELLALYKP GVVSPAKK
Length:278
Mass (Da):32,100
Last modified:July 21, 1986 - v1
Checksum:iB2FA5D1FDC863CB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01600 Genomic DNA. Translation: AAA23664.1.
V00272 Genomic DNA. Translation: CAA23530.1.
Z19601 Genomic DNA. Translation: CAA79668.1.
U18997 Genomic DNA. Translation: AAA58184.1.
U00096 Genomic DNA. Translation: AAC76412.1.
AP009048 Genomic DNA. Translation: BAE77904.1.
X15162 Genomic DNA. Translation: CAA33254.1.
PIRiA00555. XYECDA.
RefSeqiNP_417846.1. NC_000913.3.
YP_492045.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76412; AAC76412; b3387.
BAE77904; BAE77904; BAE77904.
GeneIDi12932866.
947893.
KEGGiecj:Y75_p3789.
eco:b3387.
PATRICi32122206. VBIEscCol129921_3480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01600 Genomic DNA. Translation: AAA23664.1.
V00272 Genomic DNA. Translation: CAA23530.1.
Z19601 Genomic DNA. Translation: CAA79668.1.
U18997 Genomic DNA. Translation: AAA58184.1.
U00096 Genomic DNA. Translation: AAC76412.1.
AP009048 Genomic DNA. Translation: BAE77904.1.
X15162 Genomic DNA. Translation: CAA33254.1.
PIRiA00555. XYECDA.
RefSeqiNP_417846.1. NC_000913.3.
YP_492045.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2G1PX-ray1.89A/B1-278[»]
2OREX-ray2.99D/E/F1-278[»]
4GBEX-ray2.66D/E/F1-278[»]
4GOLX-ray2.57D/E/F1-278[»]
4GOMX-ray2.45D/E/F1-278[»]
4GONX-ray2.72D/E/F1-278[»]
4GOOX-ray2.70D/E/F1-278[»]
ProteinModelPortaliP0AEE8.
SMRiP0AEE8. Positions 3-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47948N.
IntActiP0AEE8. 24 interactions.
MINTiMINT-1230150.
STRINGi511145.b3387.

Chemistry

ChEMBLiCHEMBL1075075.

Protein family/group databases

REBASEi2396. M.EcoKDam.

Proteomic databases

PaxDbiP0AEE8.
PRIDEiP0AEE8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76412; AAC76412; b3387.
BAE77904; BAE77904; BAE77904.
GeneIDi12932866.
947893.
KEGGiecj:Y75_p3789.
eco:b3387.
PATRICi32122206. VBIEscCol129921_3480.

Organism-specific databases

EchoBASEiEB0200.
EcoGeneiEG10204. dam.

Phylogenomic databases

eggNOGiCOG0338.
HOGENOMiHOG000281348.
InParanoidiP0AEE8.
KOiK06223.
OMAiMNRHGFN.
OrthoDBiEOG6CS08V.
PhylomeDBiP0AEE8.

Enzyme and pathway databases

BioCyciEcoCyc:EG10204-MONOMER.
ECOL316407:JW3350-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AEE8.
PROiP0AEE8.

Gene expression databases

GenevestigatoriP0AEE8.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene."
    Brooks J.E., Blumenthal R.M., Gingeras T.R.
    Nucleic Acids Res. 11:837-851(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization of three genes in the dam-containing operon of Escherichia coli."
    Lyngstadaas A., Lobner-Olesen A., Boye E.
    Mol. Gen. Genet. 247:546-554(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The Escherichia coli dam gene is expressed as a distal gene of a new operon."
    Jonczyk P., Hines R., Smith D.W.
    Mol. Gen. Genet. 217:85-96(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-18.
    Strain: K12.
  6. "The role of the preserved sequences of Dam methylase."
    Guyot J.-B., Grassi J., Hahn U., Guschlbauer W.
    Nucleic Acids Res. 21:3183-3190(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.

Entry informationi

Entry nameiDMA_ECOLI
AccessioniPrimary (citable) accession number: P0AEE8
Secondary accession number(s): P00475, Q2M752
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: January 7, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.