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P0AEE8

- DMA_ECOLI

UniProt

P0AEE8 - DMA_ECOLI

Protein

DNA adenine methylase

Gene

dam

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101S-adenosyl-L-methionineBy similarity
    Binding sitei14 – 141S-adenosyl-L-methionine; via amide nitrogenBy similarity
    Binding sitei54 – 541S-adenosyl-L-methionineBy similarity
    Binding sitei181 – 1811S-adenosyl-L-methionineBy similarity

    GO - Molecular functioni

    1. DNA binding Source: EcoCyc
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: EcoliWiki

    GO - Biological processi

    1. DNA-dependent DNA replication Source: EcoliWiki
    2. DNA methylation Source: EcoCyc
    3. mismatch repair Source: EcoliWiki

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10204-MONOMER.
    ECOL316407:JW3350-MONOMER.

    Protein family/group databases

    REBASEi2396. M.EcoKDam.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA adenine methylase (EC:2.1.1.72)
    Alternative name(s):
    DNA adenine methyltransferase
    Deoxyadenosyl-methyltransferase
    M.EcoDam
    Gene namesi
    Name:dam
    Ordered Locus Names:b3387, JW3350
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10204. dam.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 278278DNA adenine methylasePRO_0000087991Add
    BLAST

    Proteomic databases

    PaxDbiP0AEE8.
    PRIDEiP0AEE8.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AEE8.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-47948N.
    IntActiP0AEE8. 24 interactions.
    MINTiMINT-1230150.
    STRINGi511145.b3387.

    Structurei

    Secondary structure

    1
    278
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni11 – 133
    Helixi15 – 173
    Helixi18 – 247
    Beta strandi29 – 335
    Helixi40 – 434
    Beta strandi48 – 558
    Helixi57 – 6812
    Helixi70 – 789
    Helixi79 – 813
    Helixi83 – 853
    Helixi88 – 10013
    Helixi104 – 11714
    Helixi119 – 1213
    Helixi145 – 15410
    Helixi155 – 1573
    Beta strandi158 – 1625
    Helixi165 – 1695
    Beta strandi176 – 1805
    Helixi203 – 21816
    Beta strandi223 – 2286
    Helixi231 – 2366
    Turni237 – 2393
    Beta strandi240 – 2456
    Beta strandi263 – 2686

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2G1PX-ray1.89A/B1-278[»]
    2OREX-ray2.99D/E/F1-278[»]
    4GBEX-ray2.66D/E/F1-278[»]
    4GOLX-ray2.57D/E/F1-278[»]
    4GOMX-ray2.45D/E/F1-278[»]
    4GONX-ray2.72D/E/F1-278[»]
    4GOOX-ray2.70D/E/F1-278[»]
    ProteinModelPortaliP0AEE8.
    SMRiP0AEE8. Positions 3-270.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AEE8.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0338.
    HOGENOMiHOG000281348.
    KOiK06223.
    OMAiKFTREIY.
    OrthoDBiEOG6CS08V.
    PhylomeDBiP0AEE8.

    Family and domain databases

    Gene3Di1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProiIPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00571. dam. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AEE8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY    50
    ILADINSDLI SLYNIVKMRT DEYVQAAREL FVPETNCAEV YYQFREEFNK 100
    SQDPFRRAVL FLYLNRYGYN GLCRYNLRGE FNVPFGRYKK PYFPEAELYH 150
    FAEKAQNAFF YCESYADSMA RADDASVVYC DPPYAPLSAT ANFTAYHTNS 200
    FTLEQQAHLA EIAEGLVERH IPVLISNHDT MLTREWYQRA KLHVVKVRRS 250
    ISSNGGTRKK VDELLALYKP GVVSPAKK 278
    Length:278
    Mass (Da):32,100
    Last modified:July 21, 1986 - v1
    Checksum:iB2FA5D1FDC863CB9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01600 Genomic DNA. Translation: AAA23664.1.
    V00272 Genomic DNA. Translation: CAA23530.1.
    Z19601 Genomic DNA. Translation: CAA79668.1.
    U18997 Genomic DNA. Translation: AAA58184.1.
    U00096 Genomic DNA. Translation: AAC76412.1.
    AP009048 Genomic DNA. Translation: BAE77904.1.
    X15162 Genomic DNA. Translation: CAA33254.1.
    PIRiA00555. XYECDA.
    RefSeqiNP_417846.1. NC_000913.3.
    YP_492045.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76412; AAC76412; b3387.
    BAE77904; BAE77904; BAE77904.
    GeneIDi12932866.
    947893.
    KEGGiecj:Y75_p3789.
    eco:b3387.
    PATRICi32122206. VBIEscCol129921_3480.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01600 Genomic DNA. Translation: AAA23664.1 .
    V00272 Genomic DNA. Translation: CAA23530.1 .
    Z19601 Genomic DNA. Translation: CAA79668.1 .
    U18997 Genomic DNA. Translation: AAA58184.1 .
    U00096 Genomic DNA. Translation: AAC76412.1 .
    AP009048 Genomic DNA. Translation: BAE77904.1 .
    X15162 Genomic DNA. Translation: CAA33254.1 .
    PIRi A00555. XYECDA.
    RefSeqi NP_417846.1. NC_000913.3.
    YP_492045.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2G1P X-ray 1.89 A/B 1-278 [» ]
    2ORE X-ray 2.99 D/E/F 1-278 [» ]
    4GBE X-ray 2.66 D/E/F 1-278 [» ]
    4GOL X-ray 2.57 D/E/F 1-278 [» ]
    4GOM X-ray 2.45 D/E/F 1-278 [» ]
    4GON X-ray 2.72 D/E/F 1-278 [» ]
    4GOO X-ray 2.70 D/E/F 1-278 [» ]
    ProteinModelPortali P0AEE8.
    SMRi P0AEE8. Positions 3-270.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47948N.
    IntActi P0AEE8. 24 interactions.
    MINTi MINT-1230150.
    STRINGi 511145.b3387.

    Chemistry

    ChEMBLi CHEMBL1075075.

    Protein family/group databases

    REBASEi 2396. M.EcoKDam.

    Proteomic databases

    PaxDbi P0AEE8.
    PRIDEi P0AEE8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76412 ; AAC76412 ; b3387 .
    BAE77904 ; BAE77904 ; BAE77904 .
    GeneIDi 12932866.
    947893.
    KEGGi ecj:Y75_p3789.
    eco:b3387.
    PATRICi 32122206. VBIEscCol129921_3480.

    Organism-specific databases

    EchoBASEi EB0200.
    EcoGenei EG10204. dam.

    Phylogenomic databases

    eggNOGi COG0338.
    HOGENOMi HOG000281348.
    KOi K06223.
    OMAi KFTREIY.
    OrthoDBi EOG6CS08V.
    PhylomeDBi P0AEE8.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10204-MONOMER.
    ECOL316407:JW3350-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0AEE8.
    PROi P0AEE8.

    Gene expression databases

    Genevestigatori P0AEE8.

    Family and domain databases

    Gene3Di 1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProi IPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00571. dam. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene."
      Brooks J.E., Blumenthal R.M., Gingeras T.R.
      Nucleic Acids Res. 11:837-851(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Characterization of three genes in the dam-containing operon of Escherichia coli."
      Lyngstadaas A., Lobner-Olesen A., Boye E.
      Mol. Gen. Genet. 247:546-554(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "The Escherichia coli dam gene is expressed as a distal gene of a new operon."
      Jonczyk P., Hines R., Smith D.W.
      Mol. Gen. Genet. 217:85-96(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-18.
      Strain: K12.
    6. "The role of the preserved sequences of Dam methylase."
      Guyot J.-B., Grassi J., Hahn U., Guschlbauer W.
      Nucleic Acids Res. 21:3183-3190(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.

    Entry informationi

    Entry nameiDMA_ECOLI
    AccessioniPrimary (citable) accession number: P0AEE8
    Secondary accession number(s): P00475, Q2M752
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3