DNA adenine methylase - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0AEE8 (DMA_ECOLI)

Last modified February 9, 2010. Version 44. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    DNA adenine methylase
    EC=2.1.1.72
Alternative name(s):
    M.EcoDam
    Deoxyadenosyl-methyltransferase
    DNA adenine methyltransferase

Gene names

Name:	dam
Ordered Locus Names:	b3387, JW3350

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

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Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from mutant phenotype. Source: UniProtKB mismatch repair Inferred from direct assay. Source: UniProtKB
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 278

278

DNA adenine methylase

PRO_0000087991

Sites

Binding site

S-adenosyl-L-methionine By similarity

Binding site

S-adenosyl-L-methionine; via amide nitrogen By similarity

Binding site

S-adenosyl-L-methionine By similarity

Binding site

181

S-adenosyl-L-methionine By similarity

Secondary structure

278

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P0AEE8-1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: B2FA5D1FDC863CB9
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FASTA

278

32,100

        10         20         30         40         50         60 
MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY ILADINSDLI 

        70         80         90        100        110        120 
SLYNIVKMRT DEYVQAAREL FVPETNCAEV YYQFREEFNK SQDPFRRAVL FLYLNRYGYN 

       130        140        150        160        170        180 
GLCRYNLRGE FNVPFGRYKK PYFPEAELYH FAEKAQNAFF YCESYADSMA RADDASVVYC 

       190        200        210        220        230        240 
DPPYAPLSAT ANFTAYHTNS FTLEQQAHLA EIAEGLVERH IPVLISNHDT MLTREWYQRA 

       250        260        270 
KLHVVKVRRS ISSNGGTRKK VDELLALYKP GVVSPAKK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene." Brooks J.E., Blumenthal R.M., Gingeras T.R. Nucleic Acids Res. 11:837-851(1983) [PubMed: 6300769] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Characterization of three genes in the dam-containing operon of Escherichia coli." Lyngstadaas A., Lobner-Olesen A., Boye E. Mol. Gen. Genet. 247:546-554(1995) [PubMed: 7603433] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The Escherichia coli dam gene is expressed as a distal gene of a new operon." Jonczyk P., Hines R., Smith D.W. Mol. Gen. Genet. 217:85-96(1989) [PubMed: 2549371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-18. Strain: K12.
[6]	"The role of the preserved sequences of Dam methylase." Guyot J.-B., Grassi J., Hahn U., Guschlbauer W. Nucleic Acids Res. 21:3183-3190(1993) [PubMed: 8341592] [Abstract] Cited for: MUTAGENESIS.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J01600 Genomic DNA. Translation: AAA23664.1.
V00272 Genomic DNA. Translation: CAA23530.1.
Z19601 Genomic DNA. Translation: CAA79668.1.
U18997 Genomic DNA. Translation: AAA58184.1.
U00096 Genomic DNA. Translation: AAC76412.1.
AP009048 Genomic DNA. Translation: BAE77904.1.
X15162 Genomic DNA. Translation: CAA33254.1.

PIR

XYECDA. A00555.

RefSeq

AP_004403.1.
NP_417846.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2G1P	X-ray	1.89	A/B	1-278	[»]
2ORE	X-ray	2.99	D/E/F	1-278	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P0AEE8.

Protein family/group databases

REBASE

2396. M.EcoKDam.

Genome annotation databases

GeneID

947893.

GenomeReviews

Gene locus JW3350 in contig AP009048_GR.
Gene locus b3387 in contig U00096_GR.

KEGG

ecj:JW3350.
eco:b3387.

Organism-specific databases

EchoBASE

EB0200.

EcoGene

EG10204. dam.

CMR

Search...

Phylogenomic databases

eggNOG

COG0338.

HOGENOM

HBG586381.

OMA

WAGGKFR.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10204-MONOMER.
ECOL168927:B3387-MONOMER.

Gene expression databases

Genevestigator

P0AEE8.

Family and domain databases

InterPro

IPR002294. D12N6_MeTrfase.
IPR012326. Dam.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
[Graphical view]

Pfam

PF02086. MethyltransfD12. 1 hit.
[Graphical view]

PRINTS

PR00505. D12N6MTFRASE.

TIGRFAMs

TIGR00571. dam. 1 hit.

PROSITE

PS00092. N6_MTASE. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

DMA_ECOLI

Accession

Primary (citable) accession number: P0AEE8
Secondary accession number(s): P00475, Q2M752

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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