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Protein

DNA adenine methylase

Gene

dam

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.

Catalytic activityi

S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10S-adenosyl-L-methionineBy similarity1
Binding sitei14S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei54S-adenosyl-L-methionineBy similarity1
Binding sitei181S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

  • S-adenosyl-L-methionine binding Source: EcoCyc
  • sequence-specific DNA binding Source: EcoCyc
  • site-specific DNA-methyltransferase (adenine-specific) activity Source: EcoCyc

GO - Biological processi

  • bacterial-type DNA replication initiation Source: EcoCyc
  • DNA-dependent DNA replication Source: EcoliWiki
  • DNA methylation on adenine Source: EcoCyc
  • mismatch repair Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG10204-MONOMER.
ECOL316407:JW3350-MONOMER.
MetaCyc:EG10204-MONOMER.
BRENDAi2.1.1.72. 2026.

Protein family/group databases

REBASEi2396. M.EcoKDam.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.EcoDam
Gene namesi
Name:dam
Ordered Locus Names:b3387, JW3350
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10204. dam.

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075075.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879911 – 278DNA adenine methylaseAdd BLAST278

Proteomic databases

PaxDbiP0AEE8.
PRIDEiP0AEE8.

Interactioni

Protein-protein interaction databases

BioGridi4261860. 125 interactors.
DIPiDIP-47948N.
IntActiP0AEE8. 24 interactors.
MINTiMINT-1230150.
STRINGi511145.b3387.

Structurei

Secondary structure

1278
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni11 – 13Combined sources3
Helixi15 – 17Combined sources3
Helixi18 – 24Combined sources7
Beta strandi29 – 33Combined sources5
Helixi40 – 43Combined sources4
Beta strandi48 – 55Combined sources8
Helixi57 – 68Combined sources12
Helixi70 – 78Combined sources9
Helixi79 – 81Combined sources3
Helixi83 – 85Combined sources3
Helixi88 – 100Combined sources13
Helixi104 – 117Combined sources14
Helixi119 – 121Combined sources3
Helixi145 – 154Combined sources10
Helixi155 – 157Combined sources3
Beta strandi158 – 162Combined sources5
Helixi165 – 169Combined sources5
Beta strandi176 – 180Combined sources5
Helixi203 – 218Combined sources16
Beta strandi223 – 228Combined sources6
Helixi231 – 236Combined sources6
Turni237 – 239Combined sources3
Beta strandi240 – 245Combined sources6
Beta strandi263 – 268Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G1PX-ray1.89A/B1-278[»]
2OREX-ray2.99D/E/F1-278[»]
4GBEX-ray2.66D/E/F1-278[»]
4GOLX-ray2.57D/E/F1-278[»]
4GOMX-ray2.45D/E/F1-278[»]
4GONX-ray2.72D/E/F1-278[»]
4GOOX-ray2.70D/E/F1-278[»]
4RTJX-ray1.99A1-278[»]
4RTKX-ray1.96A1-278[»]
4RTLX-ray2.19A1-278[»]
4RTMX-ray2.50A1-278[»]
4RTNX-ray2.59A1-278[»]
4RTOX-ray2.69A1-278[»]
4RTPX-ray2.39A1-278[»]
4RTQX-ray2.00A1-278[»]
4RTRX-ray2.39A1-278[»]
4RTSX-ray2.49A1-278[»]
ProteinModelPortaliP0AEE8.
SMRiP0AEE8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEE8.

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiENOG4105DFE. Bacteria.
COG0338. LUCA.
HOGENOMiHOG000281348.
InParanoidiP0AEE8.
KOiK06223.
OMAiMNRHGFN.
PhylomeDBiP0AEE8.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY
60 70 80 90 100
ILADINSDLI SLYNIVKMRT DEYVQAAREL FVPETNCAEV YYQFREEFNK
110 120 130 140 150
SQDPFRRAVL FLYLNRYGYN GLCRYNLRGE FNVPFGRYKK PYFPEAELYH
160 170 180 190 200
FAEKAQNAFF YCESYADSMA RADDASVVYC DPPYAPLSAT ANFTAYHTNS
210 220 230 240 250
FTLEQQAHLA EIAEGLVERH IPVLISNHDT MLTREWYQRA KLHVVKVRRS
260 270
ISSNGGTRKK VDELLALYKP GVVSPAKK
Length:278
Mass (Da):32,100
Last modified:July 21, 1986 - v1
Checksum:iB2FA5D1FDC863CB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01600 Genomic DNA. Translation: AAA23664.1.
V00272 Genomic DNA. Translation: CAA23530.1.
Z19601 Genomic DNA. Translation: CAA79668.1.
U18997 Genomic DNA. Translation: AAA58184.1.
U00096 Genomic DNA. Translation: AAC76412.1.
AP009048 Genomic DNA. Translation: BAE77904.1.
X15162 Genomic DNA. Translation: CAA33254.1.
PIRiA00555. XYECDA.
RefSeqiNP_417846.1. NC_000913.3.
WP_000742143.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76412; AAC76412; b3387.
BAE77904; BAE77904; BAE77904.
GeneIDi947893.
KEGGiecj:JW3350.
eco:b3387.
PATRICi32122206. VBIEscCol129921_3480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01600 Genomic DNA. Translation: AAA23664.1.
V00272 Genomic DNA. Translation: CAA23530.1.
Z19601 Genomic DNA. Translation: CAA79668.1.
U18997 Genomic DNA. Translation: AAA58184.1.
U00096 Genomic DNA. Translation: AAC76412.1.
AP009048 Genomic DNA. Translation: BAE77904.1.
X15162 Genomic DNA. Translation: CAA33254.1.
PIRiA00555. XYECDA.
RefSeqiNP_417846.1. NC_000913.3.
WP_000742143.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G1PX-ray1.89A/B1-278[»]
2OREX-ray2.99D/E/F1-278[»]
4GBEX-ray2.66D/E/F1-278[»]
4GOLX-ray2.57D/E/F1-278[»]
4GOMX-ray2.45D/E/F1-278[»]
4GONX-ray2.72D/E/F1-278[»]
4GOOX-ray2.70D/E/F1-278[»]
4RTJX-ray1.99A1-278[»]
4RTKX-ray1.96A1-278[»]
4RTLX-ray2.19A1-278[»]
4RTMX-ray2.50A1-278[»]
4RTNX-ray2.59A1-278[»]
4RTOX-ray2.69A1-278[»]
4RTPX-ray2.39A1-278[»]
4RTQX-ray2.00A1-278[»]
4RTRX-ray2.39A1-278[»]
4RTSX-ray2.49A1-278[»]
ProteinModelPortaliP0AEE8.
SMRiP0AEE8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261860. 125 interactors.
DIPiDIP-47948N.
IntActiP0AEE8. 24 interactors.
MINTiMINT-1230150.
STRINGi511145.b3387.

Chemistry databases

ChEMBLiCHEMBL1075075.

Protein family/group databases

REBASEi2396. M.EcoKDam.

Proteomic databases

PaxDbiP0AEE8.
PRIDEiP0AEE8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76412; AAC76412; b3387.
BAE77904; BAE77904; BAE77904.
GeneIDi947893.
KEGGiecj:JW3350.
eco:b3387.
PATRICi32122206. VBIEscCol129921_3480.

Organism-specific databases

EchoBASEiEB0200.
EcoGeneiEG10204. dam.

Phylogenomic databases

eggNOGiENOG4105DFE. Bacteria.
COG0338. LUCA.
HOGENOMiHOG000281348.
InParanoidiP0AEE8.
KOiK06223.
OMAiMNRHGFN.
PhylomeDBiP0AEE8.

Enzyme and pathway databases

BioCyciEcoCyc:EG10204-MONOMER.
ECOL316407:JW3350-MONOMER.
MetaCyc:EG10204-MONOMER.
BRENDAi2.1.1.72. 2026.

Miscellaneous databases

EvolutionaryTraceiP0AEE8.
PROiP0AEE8.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDMA_ECOLI
AccessioniPrimary (citable) accession number: P0AEE8
Secondary accession number(s): P00475, Q2M752
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.