Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0AEE8

- DMA_ECOLI

UniProt

P0AEE8 - DMA_ECOLI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DNA adenine methylase

Gene

dam

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101S-adenosyl-L-methionineBy similarity
Binding sitei14 – 141S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei54 – 541S-adenosyl-L-methionineBy similarity
Binding sitei181 – 1811S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

  1. DNA binding Source: EcoCyc
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: EcoliWiki

GO - Biological processi

  1. DNA-dependent DNA replication Source: EcoliWiki
  2. DNA methylation Source: EcoCyc
  3. mismatch repair Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG10204-MONOMER.
ECOL316407:JW3350-MONOMER.

Protein family/group databases

REBASEi2396. M.EcoKDam.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.EcoDam
Gene namesi
Name:dam
Ordered Locus Names:b3387, JW3350
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10204. dam.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278DNA adenine methylasePRO_0000087991Add
BLAST

Proteomic databases

PaxDbiP0AEE8.
PRIDEiP0AEE8.

Expressioni

Gene expression databases

GenevestigatoriP0AEE8.

Interactioni

Protein-protein interaction databases

DIPiDIP-47948N.
IntActiP0AEE8. 24 interactions.
MINTiMINT-1230150.
STRINGi511145.b3387.

Structurei

Secondary structure

1
278
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni11 – 133
Helixi15 – 173
Helixi18 – 247
Beta strandi29 – 335
Helixi40 – 434
Beta strandi48 – 558
Helixi57 – 6812
Helixi70 – 789
Helixi79 – 813
Helixi83 – 853
Helixi88 – 10013
Helixi104 – 11714
Helixi119 – 1213
Helixi145 – 15410
Helixi155 – 1573
Beta strandi158 – 1625
Helixi165 – 1695
Beta strandi176 – 1805
Helixi203 – 21816
Beta strandi223 – 2286
Helixi231 – 2366
Turni237 – 2393
Beta strandi240 – 2456
Beta strandi263 – 2686

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2G1PX-ray1.89A/B1-278[»]
2OREX-ray2.99D/E/F1-278[»]
4GBEX-ray2.66D/E/F1-278[»]
4GOLX-ray2.57D/E/F1-278[»]
4GOMX-ray2.45D/E/F1-278[»]
4GONX-ray2.72D/E/F1-278[»]
4GOOX-ray2.70D/E/F1-278[»]
ProteinModelPortaliP0AEE8.
SMRiP0AEE8. Positions 3-270.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEE8.

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0338.
HOGENOMiHOG000281348.
InParanoidiP0AEE8.
KOiK06223.
OMAiKFTREIY.
OrthoDBiEOG6CS08V.
PhylomeDBiP0AEE8.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEE8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY
60 70 80 90 100
ILADINSDLI SLYNIVKMRT DEYVQAAREL FVPETNCAEV YYQFREEFNK
110 120 130 140 150
SQDPFRRAVL FLYLNRYGYN GLCRYNLRGE FNVPFGRYKK PYFPEAELYH
160 170 180 190 200
FAEKAQNAFF YCESYADSMA RADDASVVYC DPPYAPLSAT ANFTAYHTNS
210 220 230 240 250
FTLEQQAHLA EIAEGLVERH IPVLISNHDT MLTREWYQRA KLHVVKVRRS
260 270
ISSNGGTRKK VDELLALYKP GVVSPAKK
Length:278
Mass (Da):32,100
Last modified:July 21, 1986 - v1
Checksum:iB2FA5D1FDC863CB9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01600 Genomic DNA. Translation: AAA23664.1.
V00272 Genomic DNA. Translation: CAA23530.1.
Z19601 Genomic DNA. Translation: CAA79668.1.
U18997 Genomic DNA. Translation: AAA58184.1.
U00096 Genomic DNA. Translation: AAC76412.1.
AP009048 Genomic DNA. Translation: BAE77904.1.
X15162 Genomic DNA. Translation: CAA33254.1.
PIRiA00555. XYECDA.
RefSeqiNP_417846.1. NC_000913.3.
YP_492045.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76412; AAC76412; b3387.
BAE77904; BAE77904; BAE77904.
GeneIDi12932866.
947893.
KEGGiecj:Y75_p3789.
eco:b3387.
PATRICi32122206. VBIEscCol129921_3480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01600 Genomic DNA. Translation: AAA23664.1 .
V00272 Genomic DNA. Translation: CAA23530.1 .
Z19601 Genomic DNA. Translation: CAA79668.1 .
U18997 Genomic DNA. Translation: AAA58184.1 .
U00096 Genomic DNA. Translation: AAC76412.1 .
AP009048 Genomic DNA. Translation: BAE77904.1 .
X15162 Genomic DNA. Translation: CAA33254.1 .
PIRi A00555. XYECDA.
RefSeqi NP_417846.1. NC_000913.3.
YP_492045.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2G1P X-ray 1.89 A/B 1-278 [» ]
2ORE X-ray 2.99 D/E/F 1-278 [» ]
4GBE X-ray 2.66 D/E/F 1-278 [» ]
4GOL X-ray 2.57 D/E/F 1-278 [» ]
4GOM X-ray 2.45 D/E/F 1-278 [» ]
4GON X-ray 2.72 D/E/F 1-278 [» ]
4GOO X-ray 2.70 D/E/F 1-278 [» ]
ProteinModelPortali P0AEE8.
SMRi P0AEE8. Positions 3-270.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47948N.
IntActi P0AEE8. 24 interactions.
MINTi MINT-1230150.
STRINGi 511145.b3387.

Chemistry

ChEMBLi CHEMBL1075075.

Protein family/group databases

REBASEi 2396. M.EcoKDam.

Proteomic databases

PaxDbi P0AEE8.
PRIDEi P0AEE8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76412 ; AAC76412 ; b3387 .
BAE77904 ; BAE77904 ; BAE77904 .
GeneIDi 12932866.
947893.
KEGGi ecj:Y75_p3789.
eco:b3387.
PATRICi 32122206. VBIEscCol129921_3480.

Organism-specific databases

EchoBASEi EB0200.
EcoGenei EG10204. dam.

Phylogenomic databases

eggNOGi COG0338.
HOGENOMi HOG000281348.
InParanoidi P0AEE8.
KOi K06223.
OMAi KFTREIY.
OrthoDBi EOG6CS08V.
PhylomeDBi P0AEE8.

Enzyme and pathway databases

BioCyci EcoCyc:EG10204-MONOMER.
ECOL316407:JW3350-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0AEE8.
PROi P0AEE8.

Gene expression databases

Genevestigatori P0AEE8.

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProi IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 1 hit.
[Graphical view ]
PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene."
    Brooks J.E., Blumenthal R.M., Gingeras T.R.
    Nucleic Acids Res. 11:837-851(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization of three genes in the dam-containing operon of Escherichia coli."
    Lyngstadaas A., Lobner-Olesen A., Boye E.
    Mol. Gen. Genet. 247:546-554(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The Escherichia coli dam gene is expressed as a distal gene of a new operon."
    Jonczyk P., Hines R., Smith D.W.
    Mol. Gen. Genet. 217:85-96(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-18.
    Strain: K12.
  6. "The role of the preserved sequences of Dam methylase."
    Guyot J.-B., Grassi J., Hahn U., Guschlbauer W.
    Nucleic Acids Res. 21:3183-3190(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.

Entry informationi

Entry nameiDMA_ECOLI
AccessioniPrimary (citable) accession number: P0AEE8
Secondary accession number(s): P00475, Q2M752
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3