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Protein

D-galactose-binding periplasmic protein

Gene

mglB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein is involved in the active transport of galactose and glucose. It plays a role in the chemotaxis towards the two sugars by interacting with the trg chemoreceptor.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei97Interacts with membrane-bound trg signal transducer1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi157 – 165First part of site9
Calcium bindingi227 – 228Second part of site2

GO - Molecular functioni

GO - Biological processi

  • chemotaxis Source: EcoCyc
  • galactose transmembrane transport Source: EcoCyc
  • methylgalactoside transport Source: EcoCyc

Keywordsi

Biological processChemotaxis, Sugar transport, Transport
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:MGLB-MONOMER
MetaCyc:MGLB-MONOMER

Protein family/group databases

TCDBi3.A.1.2.3 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
D-galactose-binding periplasmic protein
Short name:
GBP
Alternative name(s):
D-galactose/ D-glucose-binding protein
Short name:
GGBP
Gene namesi
Name:mglB
Ordered Locus Names:b2150, JW2137
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10593 mglB

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi97G → D: Improductive interaction with trg. 1

Chemistry databases

DrugBankiDB02379 Beta-D-Glucose

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 232 PublicationsAdd BLAST23
ChainiPRO_000003172224 – 332D-galactose-binding periplasmic proteinAdd BLAST309

Proteomic databases

EPDiP0AEE5
PaxDbiP0AEE5
PRIDEiP0AEE5

2D gel databases

SWISS-2DPAGEiP0AEE5

Interactioni

Protein-protein interaction databases

BioGridi4259172, 16 interactors
DIPiDIP-35953N
IntActiP0AEE5, 8 interactors
STRINGi316385.ECDH10B_2307

Structurei

Secondary structure

1332
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 33Combined sources8
Helixi38 – 52Combined sources15
Beta strandi57 – 63Combined sources7
Helixi68 – 80Combined sources13
Beta strandi84 – 88Combined sources5
Helixi93 – 95Combined sources3
Helixi96 – 104Combined sources9
Turni105 – 107Combined sources3
Beta strandi110 – 115Combined sources6
Helixi119 – 123Combined sources5
Beta strandi128 – 132Combined sources5
Helixi135 – 152Combined sources18
Helixi154 – 156Combined sources3
Beta strandi161 – 170Combined sources10
Helixi176 – 191Combined sources16
Beta strandi196 – 203Combined sources8
Helixi208 – 219Combined sources12
Helixi224 – 226Combined sources3
Beta strandi229 – 234Combined sources6
Helixi235 – 247Combined sources13
Helixi262 – 269Combined sources8
Beta strandi271 – 273Combined sources3
Beta strandi275 – 278Combined sources4
Helixi281 – 296Combined sources16
Turni301 – 304Combined sources4
Beta strandi313 – 315Combined sources3
Beta strandi319 – 321Combined sources3
Turni323 – 325Combined sources3
Helixi326 – 328Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GLGX-ray2.00A24-332[»]
2FVYX-ray0.92A24-332[»]
2FW0X-ray1.55A24-332[»]
2GBPX-ray1.90A24-332[»]
2HPHX-ray1.33A24-332[»]
2IPLX-ray1.20A24-332[»]
2IPMX-ray1.12A24-332[»]
2IPNX-ray1.15A24-332[»]
2QW1X-ray1.70A24-332[»]
ProteinModelPortaliP0AEE5
SMRiP0AEE5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEE5

Family & Domainsi

Domaini

The calcium-binding site is structurally similar to that of EF-hand proteins, but is in two parts, with the last calcium ligand provided by Glu-228.

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105EMA Bacteria
COG1879 LUCA
HOGENOMiHOG000212345
InParanoidiP0AEE5
KOiK10540
OMAiKVYYVGA
PhylomeDBiP0AEE5

Family and domain databases

InterProiView protein in InterPro
IPR028082 Peripla_BP_I
IPR025997 SBP_2_dom
PfamiView protein in Pfam
PF13407 Peripla_BP_4, 1 hit
SUPFAMiSSF53822 SSF53822, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKVLTLSA VMASMLFGAA AHAADTRIGV TIYKYDDNFM SVVRKAIEQD
60 70 80 90 100
AKAAPDVQLL MNDSQNDQSK QNDQIDVLLA KGVKALAINL VDPAAAGTVI
110 120 130 140 150
EKARGQNVPV VFFNKEPSRK ALDSYDKAYY VGTDSKESGI IQGDLIAKHW
160 170 180 190 200
AANQGWDLNK DGQIQFVLLK GEPGHPDAEA RTTYVIKELN DKGIKTEQLQ
210 220 230 240 250
LDTAMWDTAQ AKDKMDAWLS GPNANKIEVV IANNDAMAMG AVEALKAHNK
260 270 280 290 300
SSIPVFGVDA LPEALALVKS GALAGTVLND ANNQAKATFD LAKNLADGKG
310 320 330
AADGTNWKID NKVVRVPYVG VDKDNLAEFS KK
Length:332
Mass (Da):35,713
Last modified:August 13, 1987 - v1
Checksum:i9A57E86C423AA6D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59444 Genomic DNA Translation: AAA24169.1
U00007 Genomic DNA Translation: AAA60523.1
X05646 Genomic DNA Translation: CAA29132.1
U00096 Genomic DNA Translation: AAC75211.1
AP009048 Genomic DNA Translation: BAE76627.1
K00419 Genomic DNA Translation: AAA24172.1
PIRiA37277 JGECG
RefSeqiNP_416655.1, NC_000913.3
WP_001036964.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75211; AAC75211; b2150
BAE76627; BAE76627; BAE76627
GeneIDi949041
KEGGiecj:JW2137
eco:b2150
PATRICifig|1411691.4.peg.91

Similar proteinsi

Entry informationi

Entry nameiDGAL_ECOLI
AccessioniPrimary (citable) accession number: P0AEE5
Secondary accession number(s): P02927, P17775, Q2MAS9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: April 25, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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