Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

D-galactose-binding periplasmic protein

Gene

mglB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is involved in the active transport of galactose and glucose. It plays a role in the chemotaxis towards the two sugars by interacting with the trg chemoreceptor.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei97 – 971Interacts with membrane-bound trg signal transducer

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi157 – 1659First part of site
Calcium bindingi227 – 2282Second part of site

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Chemotaxis, Sugar transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:MGLB-MONOMER.
ECOL316407:JW2137-MONOMER.
MetaCyc:MGLB-MONOMER.

Protein family/group databases

TCDBi3.A.1.2.3. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
D-galactose-binding periplasmic protein
Short name:
GBP
Alternative name(s):
D-galactose/ D-glucose-binding protein
Short name:
GGBP
Gene namesi
Name:mglB
Ordered Locus Names:b2150, JW2137
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10593. mglB.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi97 – 971G → D: Improductive interaction with trg.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23232 PublicationsAdd
BLAST
Chaini24 – 332309D-galactose-binding periplasmic proteinPRO_0000031722Add
BLAST

Proteomic databases

EPDiP0AEE5.
PaxDbiP0AEE5.
PRIDEiP0AEE5.

2D gel databases

SWISS-2DPAGEP0AEE5.

Interactioni

Protein-protein interaction databases

BioGridi4259172. 12 interactions.
DIPiDIP-35953N.
IntActiP0AEE5. 8 interactions.
MINTiMINT-1274707.
STRINGi511145.b2150.

Structurei

Secondary structure

1
332
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi26 – 338Combined sources
Helixi38 – 5215Combined sources
Beta strandi57 – 637Combined sources
Helixi68 – 8013Combined sources
Beta strandi84 – 885Combined sources
Helixi93 – 953Combined sources
Helixi96 – 1049Combined sources
Turni105 – 1073Combined sources
Beta strandi110 – 1156Combined sources
Helixi119 – 1235Combined sources
Beta strandi128 – 1325Combined sources
Helixi135 – 15218Combined sources
Helixi154 – 1563Combined sources
Beta strandi161 – 17010Combined sources
Helixi176 – 19116Combined sources
Beta strandi196 – 2038Combined sources
Helixi208 – 21912Combined sources
Helixi224 – 2263Combined sources
Beta strandi229 – 2346Combined sources
Helixi235 – 24713Combined sources
Helixi262 – 2698Combined sources
Beta strandi271 – 2733Combined sources
Beta strandi275 – 2784Combined sources
Helixi281 – 29616Combined sources
Turni301 – 3044Combined sources
Beta strandi313 – 3153Combined sources
Beta strandi319 – 3213Combined sources
Turni323 – 3253Combined sources
Helixi326 – 3283Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GLGX-ray2.00A24-332[»]
2FVYX-ray0.92A24-332[»]
2FW0X-ray1.55A24-332[»]
2GBPX-ray1.90A24-332[»]
2HPHX-ray1.33A24-332[»]
2IPLX-ray1.20A24-332[»]
2IPMX-ray1.12A24-332[»]
2IPNX-ray1.15A24-332[»]
2QW1X-ray1.70A24-332[»]
ProteinModelPortaliP0AEE5.
SMRiP0AEE5. Positions 25-329.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEE5.

Family & Domainsi

Domaini

The calcium-binding site is structurally similar to that of EF-hand proteins, but is in two parts, with the last calcium ligand provided by Glu-228.

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105EMA. Bacteria.
COG1879. LUCA.
HOGENOMiHOG000212345.
InParanoidiP0AEE5.
KOiK10540.
OMAiAMWDTAM.
PhylomeDBiP0AEE5.

Family and domain databases

InterProiIPR028082. Peripla_BP_I.
IPR025997. SBP_2_dom.
[Graphical view]
PfamiPF13407. Peripla_BP_4. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKVLTLSA VMASMLFGAA AHAADTRIGV TIYKYDDNFM SVVRKAIEQD
60 70 80 90 100
AKAAPDVQLL MNDSQNDQSK QNDQIDVLLA KGVKALAINL VDPAAAGTVI
110 120 130 140 150
EKARGQNVPV VFFNKEPSRK ALDSYDKAYY VGTDSKESGI IQGDLIAKHW
160 170 180 190 200
AANQGWDLNK DGQIQFVLLK GEPGHPDAEA RTTYVIKELN DKGIKTEQLQ
210 220 230 240 250
LDTAMWDTAQ AKDKMDAWLS GPNANKIEVV IANNDAMAMG AVEALKAHNK
260 270 280 290 300
SSIPVFGVDA LPEALALVKS GALAGTVLND ANNQAKATFD LAKNLADGKG
310 320 330
AADGTNWKID NKVVRVPYVG VDKDNLAEFS KK
Length:332
Mass (Da):35,713
Last modified:August 13, 1987 - v1
Checksum:i9A57E86C423AA6D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59444 Genomic DNA. Translation: AAA24169.1.
U00007 Genomic DNA. Translation: AAA60523.1.
X05646 Genomic DNA. Translation: CAA29132.1.
U00096 Genomic DNA. Translation: AAC75211.1.
AP009048 Genomic DNA. Translation: BAE76627.1.
K00419 Genomic DNA. Translation: AAA24172.1.
PIRiA37277. JGECG.
RefSeqiNP_416655.1. NC_000913.3.
WP_001036964.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75211; AAC75211; b2150.
BAE76627; BAE76627; BAE76627.
GeneIDi949041.
KEGGiecj:JW2137.
eco:b2150.
PATRICi32119645. VBIEscCol129921_2233.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59444 Genomic DNA. Translation: AAA24169.1.
U00007 Genomic DNA. Translation: AAA60523.1.
X05646 Genomic DNA. Translation: CAA29132.1.
U00096 Genomic DNA. Translation: AAC75211.1.
AP009048 Genomic DNA. Translation: BAE76627.1.
K00419 Genomic DNA. Translation: AAA24172.1.
PIRiA37277. JGECG.
RefSeqiNP_416655.1. NC_000913.3.
WP_001036964.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GLGX-ray2.00A24-332[»]
2FVYX-ray0.92A24-332[»]
2FW0X-ray1.55A24-332[»]
2GBPX-ray1.90A24-332[»]
2HPHX-ray1.33A24-332[»]
2IPLX-ray1.20A24-332[»]
2IPMX-ray1.12A24-332[»]
2IPNX-ray1.15A24-332[»]
2QW1X-ray1.70A24-332[»]
ProteinModelPortaliP0AEE5.
SMRiP0AEE5. Positions 25-329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259172. 12 interactions.
DIPiDIP-35953N.
IntActiP0AEE5. 8 interactions.
MINTiMINT-1274707.
STRINGi511145.b2150.

Protein family/group databases

TCDBi3.A.1.2.3. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP0AEE5.

Proteomic databases

EPDiP0AEE5.
PaxDbiP0AEE5.
PRIDEiP0AEE5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75211; AAC75211; b2150.
BAE76627; BAE76627; BAE76627.
GeneIDi949041.
KEGGiecj:JW2137.
eco:b2150.
PATRICi32119645. VBIEscCol129921_2233.

Organism-specific databases

EchoBASEiEB0588.
EcoGeneiEG10593. mglB.

Phylogenomic databases

eggNOGiENOG4105EMA. Bacteria.
COG1879. LUCA.
HOGENOMiHOG000212345.
InParanoidiP0AEE5.
KOiK10540.
OMAiAMWDTAM.
PhylomeDBiP0AEE5.

Enzyme and pathway databases

BioCyciEcoCyc:MGLB-MONOMER.
ECOL316407:JW2137-MONOMER.
MetaCyc:MGLB-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AEE5.
PROiP0AEE5.

Family and domain databases

InterProiIPR028082. Peripla_BP_I.
IPR025997. SBP_2_dom.
[Graphical view]
PfamiPF13407. Peripla_BP_4. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDGAL_ECOLI
AccessioniPrimary (citable) accession number: P0AEE5
Secondary accession number(s): P02927, P17775, Q2MAS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.