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Protein

DNA-cytosine methyltransferase

Gene

dcm

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands.By similarity

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciECOL386585:GJFA-2663-MONOMER.
ECOO157:DCM-MONOMER.

Protein family/group databases

REBASEi5589. M.EcoKO157DcmP.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-cytosine methyltransferase (EC:2.1.1.37)
Gene namesi
Name:dcm
Ordered Locus Names:Z3054, ECs2699
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472DNA-cytosine methyltransferasePRO_0000087914Add
BLAST

Interactioni

Protein-protein interaction databases

MINTiMINT-1237409.
STRINGi155864.Z3054.

Structurei

3D structure databases

ProteinModelPortaliP0AEE0.
SMRiP0AEE0. Positions 15-466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 457371SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0270.
HOGENOMiHOG000225506.
KOiK00558.
OMAiTLWKYLY.
OrthoDBiEOG6PZXGV.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQENISVTDS YSTGNAAQAM LEKLLQIYDV KTLVAQLNGV GENHWSAAIL
60 70 80 90 100
KRALANDSAW HRLSEKEFAH LQTLLPKPPA HHPHYAFRFI DLFAGIGGIR
110 120 130 140 150
RGFESIGGQC VFTSEWNKHA VRTYKANHYC DPATHHFNED IRDITLSHKE
160 170 180 190 200
GVSDEAAAEH IRQHIPEHDV LLAGFPCQPF SLAGVSKKNS LGRAHGFACD
210 220 230 240 250
TQGTLFFDVV RIIDARRPAM FVLENVKNLK SHDQGKTFRI IMQTLDELGY
260 270 280 290 300
DVADAEDNGP DDPKIIDGKH FLPQHRERIV LVGFRRDLNL KADFTLRDIS
310 320 330 340 350
ECFPAQRVTL AQLLDPMVEA KYILTPVLWK YLYRYAKKHQ ARGNGFGYGM
360 370 380 390 400
VYPNNPQSVT RTLSARYYKD GAEILIDRGW DMATGEKDFD DPLNQQHRPR
410 420 430 440 450
RLTPRECARL MGFEAPGEAK FRIPVSDTQA YRQFGNSVVV PVFAAVAKLL
460 470
EPKIKQAVAL RQQEAQHGRR SR
Length:472
Mass (Da):53,465
Last modified:December 6, 2005 - v1
Checksum:i3635BFC001CF94B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56975.1.
BA000007 Genomic DNA. Translation: BAB36122.1.
PIRiC85814.
C90966.
RefSeqiNP_310726.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG56975; AAG56975; Z3054.
BAB36122; BAB36122; BAB36122.
GeneIDi913056.
KEGGiece:Z3054.
ecs:ECs2699.
PATRICi18354786. VBIEscCol44059_2601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56975.1.
BA000007 Genomic DNA. Translation: BAB36122.1.
PIRiC85814.
C90966.
RefSeqiNP_310726.1. NC_002695.1.

3D structure databases

ProteinModelPortaliP0AEE0.
SMRiP0AEE0. Positions 15-466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1237409.
STRINGi155864.Z3054.

Protein family/group databases

REBASEi5589. M.EcoKO157DcmP.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG56975; AAG56975; Z3054.
BAB36122; BAB36122; BAB36122.
GeneIDi913056.
KEGGiece:Z3054.
ecs:ECs2699.
PATRICi18354786. VBIEscCol44059_2601.

Phylogenomic databases

eggNOGiCOG0270.
HOGENOMiHOG000225506.
KOiK00558.
OMAiTLWKYLY.
OrthoDBiEOG6PZXGV.

Enzyme and pathway databases

BioCyciECOL386585:GJFA-2663-MONOMER.
ECOO157:DCM-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiDCM_ECO57
AccessioniPrimary (citable) accession number: P0AEE0
Secondary accession number(s): P11876
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: June 24, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.