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P0AEE0 (DCM_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-cytosine methyltransferase

EC=2.1.1.37
Gene names
Name:dcm
Ordered Locus Names:Z3054, ECs2699
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands By similarity.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 472472DNA-cytosine methyltransferase
PRO_0000087914

Regions

Domain87 – 457371SAM-dependent MTase C5-type

Sites

Active site1771 By similarity

Sequences

Sequence LengthMass (Da)Tools
P0AEE0 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 3635BFC001CF94B4

FASTA47253,465
        10         20         30         40         50         60 
MQENISVTDS YSTGNAAQAM LEKLLQIYDV KTLVAQLNGV GENHWSAAIL KRALANDSAW 

        70         80         90        100        110        120 
HRLSEKEFAH LQTLLPKPPA HHPHYAFRFI DLFAGIGGIR RGFESIGGQC VFTSEWNKHA 

       130        140        150        160        170        180 
VRTYKANHYC DPATHHFNED IRDITLSHKE GVSDEAAAEH IRQHIPEHDV LLAGFPCQPF 

       190        200        210        220        230        240 
SLAGVSKKNS LGRAHGFACD TQGTLFFDVV RIIDARRPAM FVLENVKNLK SHDQGKTFRI 

       250        260        270        280        290        300 
IMQTLDELGY DVADAEDNGP DDPKIIDGKH FLPQHRERIV LVGFRRDLNL KADFTLRDIS 

       310        320        330        340        350        360 
ECFPAQRVTL AQLLDPMVEA KYILTPVLWK YLYRYAKKHQ ARGNGFGYGM VYPNNPQSVT 

       370        380        390        400        410        420 
RTLSARYYKD GAEILIDRGW DMATGEKDFD DPLNQQHRPR RLTPRECARL MGFEAPGEAK 

       430        440        450        460        470 
FRIPVSDTQA YRQFGNSVVV PVFAAVAKLL EPKIKQAVAL RQQEAQHGRR SR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005174 Genomic DNA. Translation: AAG56975.1.
BA000007 Genomic DNA. Translation: BAB36122.1.
PIRC85814.
C90966.
RefSeqNP_288421.1. NC_002655.2.
NP_310726.1. NC_002695.1.

3D structure databases

ProteinModelPortalP0AEE0.
SMRP0AEE0. Positions 15-466.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1237409.
STRING155864.Z3054.

Protein family/group databases

REBASE5589. M.EcoKO157DcmP.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG56975; AAG56975; Z3054.
BAB36122; BAB36122; BAB36122.
GeneID913056.
961961.
KEGGece:Z3054.
ecs:ECs2699.
PATRIC18354786. VBIEscCol44059_2601.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0270.
HOGENOMHOG000225506.
KOK00558.
OMAIDGRHFL.
OrthoDBEOG6PZXGV.

Enzyme and pathway databases

BioCycECOL386585:GJFA-2663-MONOMER.
ECOO157:DCM-MONOMER.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDCM_ECO57
AccessionPrimary (citable) accession number: P0AEE0
Secondary accession number(s): P11876
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries