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Reviewed, UniProtKB/Swiss-Prot P0AED9 (DCM_ECOLI)

Last modified June 16, 2009. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    DNA-cytosine methyltransferase
    EC=2.1.1.37
Alternative name(s):
    M.EcoDcm
Gene names
Name: dcm
Synonyms: mec
Ordered Locus Names: b1961, JW1944
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the C5-methyltransferase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

aceFP069591EBI-548525,EBI-542707

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 472472DNA-cytosine methyltransferase
PRO_0000087913

Sites

Active site1771 By similarity

Sequences

Sequence LengthMass (Da)Tools
P0AED9-1 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 3635BFC001CF94B4

FASTA47253,465
        10         20         30         40         50         60 
MQENISVTDS YSTGNAAQAM LEKLLQIYDV KTLVAQLNGV GENHWSAAIL KRALANDSAW 

        70         80         90        100        110        120 
HRLSEKEFAH LQTLLPKPPA HHPHYAFRFI DLFAGIGGIR RGFESIGGQC VFTSEWNKHA 

       130        140        150        160        170        180 
VRTYKANHYC DPATHHFNED IRDITLSHKE GVSDEAAAEH IRQHIPEHDV LLAGFPCQPF 

       190        200        210        220        230        240 
SLAGVSKKNS LGRAHGFACD TQGTLFFDVV RIIDARRPAM FVLENVKNLK SHDQGKTFRI 

       250        260        270        280        290        300 
IMQTLDELGY DVADAEDNGP DDPKIIDGKH FLPQHRERIV LVGFRRDLNL KADFTLRDIS 

       310        320        330        340        350        360 
ECFPAQRVTL AQLLDPMVEA KYILTPVLWK YLYRYAKKHQ ARGNGFGYGM VYPNNPQSVT 

       370        380        390        400        410        420 
RTLSARYYKD GAEILIDRGW DMATGEKDFD DPLNQQHRPR RLTPRECARL MGFEAPGEAK 

       430        440        450        460        470 
FRIPVSDTQA YRQFGNSVVV PVFAAVAKLL EPKIKQAVAL RQQEAQHGRR SR 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the dcm locus of Escherichia coli K12."
Hanck T., Gerwin N., Fritz H.-J.
Nucleic Acids Res. 17:5844-5844(1989) [PubMed: 2527357] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A gene required for very short patch repair in Escherichia coli is adjacent to the DNA cytosine methylase gene."
Sohail A., Lieb M., Dar M., Bhagwat A.S.
J. Bacteriol. 172:4214-4221(1990) [PubMed: 2198248] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

X13330 Genomic DNA. Translation: CAA31705.1.
M32307 Unassigned DNA. Translation: AAA03723.1.
U00096 Genomic DNA. Translation: AAC75027.1.
AP009048 Genomic DNA. Translation: BAA15788.1.
PIRJS0263. A37754.
RefSeqAP_002572.1.
NP_416470.1.

3D structure databases

HSSPHSSP built from PDB template 1FJX based on UniProtKB P05102.
ModBaseSearch...

Protein-protein interaction databases

IntActP0AED9. 20 interactions.

Protein family/group databases

REBASE2397. M.EcoKDcm.

Genome annotation databases

GeneID946479.
GenomeReviewsGene locus JW1944 in contig AP009048_GR.
Gene locus b1961 in contig U00096_GR.
KEGGecj:JW1944.
eco:b1961.

Organism-specific databases

EchoBASEEB0207.
EcoGeneEG10211. dcm.
CMRSearch...

Phylogenomic databases

HOGENOMP0AED9.
OMAP0AED9. NALGRPH.

Enzyme and pathway databases

BioCycEcoCyc:EG10211-MON.

Family and domain databases

InterProIPR001525. C5_DNA_meth.
IPR018117. C5_DNA_meth_AS.
[Graphical view]
PANTHERPTHR10629. C5_DNA_meth. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDCM_ECOLI
AccessionPrimary (citable) accession number: P0AED9
Secondary accession number(s): P11876
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents