P0AED9 (DCM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-cytosine methyltransferase
EC=2.1.1.37

Alternative name(s):
M.EcoDcm

Gene names

Name:	dcm
Synonyms:	mec
Ordered Locus Names:	b1961, JW1944

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from direct assay PubMed 770462. Source: EcoCyc
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from direct assay PubMed 3011742. Source: EcoCyc DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
aceF	P06959	2	EBI-548525,EBI-542707

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	DNA-cytosine methyltransferase		PRO_0000087913
Sites
	Active site	177	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AED9 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 3635BFC001CF94B4	FASTA	472	53,465
	10 20 30 40 50 60 MQENISVTDS YSTGNAAQAM LEKLLQIYDV KTLVAQLNGV GENHWSAAIL KRALANDSAW 70 80 90 100 110 120 HRLSEKEFAH LQTLLPKPPA HHPHYAFRFI DLFAGIGGIR RGFESIGGQC VFTSEWNKHA 130 140 150 160 170 180 VRTYKANHYC DPATHHFNED IRDITLSHKE GVSDEAAAEH IRQHIPEHDV LLAGFPCQPF 190 200 210 220 230 240 SLAGVSKKNS LGRAHGFACD TQGTLFFDVV RIIDARRPAM FVLENVKNLK SHDQGKTFRI 250 260 270 280 290 300 IMQTLDELGY DVADAEDNGP DDPKIIDGKH FLPQHRERIV LVGFRRDLNL KADFTLRDIS 310 320 330 340 350 360 ECFPAQRVTL AQLLDPMVEA KYILTPVLWK YLYRYAKKHQ ARGNGFGYGM VYPNNPQSVT 370 380 390 400 410 420 RTLSARYYKD GAEILIDRGW DMATGEKDFD DPLNQQHRPR RLTPRECARL MGFEAPGEAK 430 440 450 460 470 FRIPVSDTQA YRQFGNSVVV PVFAAVAKLL EPKIKQAVAL RQQEAQHGRR SR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the dcm locus of Escherichia coli K12." Hanck T., Gerwin N., Fritz H.-J. Nucleic Acids Res. 17:5844-5844(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A gene required for very short patch repair in Escherichia coli is adjacent to the DNA cytosine methylase gene." Sohail A., Lieb M., Dar M., Bhagwat A.S. J. Bacteriol. 172:4214-4221(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13330 Genomic DNA. Translation: CAA31705.1. M32307 Unassigned DNA. Translation: AAA03723.1. U00096 Genomic DNA. Translation: AAC75027.1. AP009048 Genomic DNA. Translation: BAA15788.1.
PIR	JS0263. A37754.
RefSeq	NP_416470.1. NC_000913.2. YP_490214.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AED9.
SMR	P0AED9. Positions 15-466.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47858N.
IntAct	P0AED9. 20 interactions.
MINT	MINT-1237420.
STRING	511145.b1961.
Protein family/group databases
REBASE	2397. M.EcoKDcm.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75027; AAC75027; b1961. BAA15788; BAA15788; BAA15788.
GeneID	12931871. 946479.
KEGG	ecj:Y75_p1930. eco:b1961.
PATRIC	32119251. VBIEscCol129921_2040.
Organism-specific databases
EchoBASE	EB0207.
EcoGene	EG10211. dcm.
Phylogenomic databases
eggNOG	COG0270.
HOGENOM	HOG000225506.
KO	K00558.
OMA	KHRAAGN.
ProtClustDB	PRK10458.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10211-MONOMER. ECOL316407:JW1944-MONOMER. MetaCyc:EG10211-MONOMER.
Gene expression databases
Genevestigator	P0AED9.
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCM_ECOLI

Accession

Primary (citable) accession number: P0AED9
Secondary accession number(s): P11876

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program