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Protein

DNA-cytosine methyltransferase

Gene

dcm

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771PROSITE-ProRule annotation

GO - Molecular functioni

  • DNA (cytosine-5-)-methyltransferase activity Source: EcoCyc
  • DNA binding Source: InterPro

GO - Biological processi

  • C-5 methylation of cytosine Source: GOC
  • DNA methylation Source: EcoCyc
  • DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG10211-MONOMER.
ECOL316407:JW1944-MONOMER.
MetaCyc:EG10211-MONOMER.

Protein family/group databases

REBASEi2397. M.EcoKDcm.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-cytosine methyltransferase (EC:2.1.1.37)
Alternative name(s):
M.EcoDcm
Gene namesi
Name:dcm
Synonyms:mec
Ordered Locus Names:b1961, JW1944
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10211. dcm.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472DNA-cytosine methyltransferasePRO_0000087913Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
aceFP069592EBI-548525,EBI-542707

Protein-protein interaction databases

DIPiDIP-47858N.
IntActiP0AED9. 21 interactions.
MINTiMINT-1237420.
STRINGi511145.b1961.

Structurei

3D structure databases

ProteinModelPortaliP0AED9.
SMRiP0AED9. Positions 15-466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 457371SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0270.
HOGENOMiHOG000225506.
InParanoidiP0AED9.
KOiK00558.
OMAiTLWKYLY.
OrthoDBiEOG6PZXGV.
PhylomeDBiP0AED9.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AED9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQENISVTDS YSTGNAAQAM LEKLLQIYDV KTLVAQLNGV GENHWSAAIL
60 70 80 90 100
KRALANDSAW HRLSEKEFAH LQTLLPKPPA HHPHYAFRFI DLFAGIGGIR
110 120 130 140 150
RGFESIGGQC VFTSEWNKHA VRTYKANHYC DPATHHFNED IRDITLSHKE
160 170 180 190 200
GVSDEAAAEH IRQHIPEHDV LLAGFPCQPF SLAGVSKKNS LGRAHGFACD
210 220 230 240 250
TQGTLFFDVV RIIDARRPAM FVLENVKNLK SHDQGKTFRI IMQTLDELGY
260 270 280 290 300
DVADAEDNGP DDPKIIDGKH FLPQHRERIV LVGFRRDLNL KADFTLRDIS
310 320 330 340 350
ECFPAQRVTL AQLLDPMVEA KYILTPVLWK YLYRYAKKHQ ARGNGFGYGM
360 370 380 390 400
VYPNNPQSVT RTLSARYYKD GAEILIDRGW DMATGEKDFD DPLNQQHRPR
410 420 430 440 450
RLTPRECARL MGFEAPGEAK FRIPVSDTQA YRQFGNSVVV PVFAAVAKLL
460 470
EPKIKQAVAL RQQEAQHGRR SR
Length:472
Mass (Da):53,465
Last modified:December 6, 2005 - v1
Checksum:i3635BFC001CF94B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13330 Genomic DNA. Translation: CAA31705.1.
M32307 Unassigned DNA. Translation: AAA03723.1.
U00096 Genomic DNA. Translation: AAC75027.1.
AP009048 Genomic DNA. Translation: BAA15788.1.
PIRiA37754. JS0263.
RefSeqiNP_416470.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC75027; AAC75027; b1961.
BAA15788; BAA15788; BAA15788.
GeneIDi946479.
KEGGiecj:Y75_p1930.
eco:b1961.
PATRICi32119251. VBIEscCol129921_2040.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13330 Genomic DNA. Translation: CAA31705.1.
M32307 Unassigned DNA. Translation: AAA03723.1.
U00096 Genomic DNA. Translation: AAC75027.1.
AP009048 Genomic DNA. Translation: BAA15788.1.
PIRiA37754. JS0263.
RefSeqiNP_416470.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP0AED9.
SMRiP0AED9. Positions 15-466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47858N.
IntActiP0AED9. 21 interactions.
MINTiMINT-1237420.
STRINGi511145.b1961.

Protein family/group databases

REBASEi2397. M.EcoKDcm.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75027; AAC75027; b1961.
BAA15788; BAA15788; BAA15788.
GeneIDi946479.
KEGGiecj:Y75_p1930.
eco:b1961.
PATRICi32119251. VBIEscCol129921_2040.

Organism-specific databases

EchoBASEiEB0207.
EcoGeneiEG10211. dcm.

Phylogenomic databases

eggNOGiCOG0270.
HOGENOMiHOG000225506.
InParanoidiP0AED9.
KOiK00558.
OMAiTLWKYLY.
OrthoDBiEOG6PZXGV.
PhylomeDBiP0AED9.

Enzyme and pathway databases

BioCyciEcoCyc:EG10211-MONOMER.
ECOL316407:JW1944-MONOMER.
MetaCyc:EG10211-MONOMER.

Miscellaneous databases

PROiP0AED9.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the dcm locus of Escherichia coli K12."
    Hanck T., Gerwin N., Fritz H.-J.
    Nucleic Acids Res. 17:5844-5844(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "A gene required for very short patch repair in Escherichia coli is adjacent to the DNA cytosine methylase gene."
    Sohail A., Lieb M., Dar M., Bhagwat A.S.
    J. Bacteriol. 172:4214-4221(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiDCM_ECOLI
AccessioniPrimary (citable) accession number: P0AED9
Secondary accession number(s): P11876
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: June 24, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.