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P0AED9

- DCM_ECOLI

UniProt

P0AED9 - DCM_ECOLI

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Protein
DNA-cytosine methyltransferase
Gene
dcm, mec, b1961, JW1944
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: EcoCyc
  2. DNA binding Source: InterPro
  3. protein binding Source: IntAct

GO - Biological processi

  1. C-5 methylation of cytosine Source: GOC
  2. DNA methylation Source: EcoCyc
  3. DNA restriction-modification system Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG10211-MONOMER.
ECOL316407:JW1944-MONOMER.
MetaCyc:EG10211-MONOMER.

Protein family/group databases

REBASEi2397. M.EcoKDcm.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-cytosine methyltransferase (EC:2.1.1.37)
Alternative name(s):
M.EcoDcm
Gene namesi
Name:dcm
Synonyms:mec
Ordered Locus Names:b1961, JW1944
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10211. dcm.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472DNA-cytosine methyltransferase
PRO_0000087913Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP0AED9.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
aceFP069592EBI-548525,EBI-542707

Protein-protein interaction databases

DIPiDIP-47858N.
IntActiP0AED9. 21 interactions.
MINTiMINT-1237420.
STRINGi511145.b1961.

Structurei

3D structure databases

ProteinModelPortaliP0AED9.
SMRiP0AED9. Positions 15-466.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 457371SAM-dependent MTase C5-type
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0270.
HOGENOMiHOG000225506.
KOiK00558.
OMAiIDGRHFL.
OrthoDBiEOG6PZXGV.
PhylomeDBiP0AED9.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AED9-1 [UniParc]FASTAAdd to Basket

« Hide

MQENISVTDS YSTGNAAQAM LEKLLQIYDV KTLVAQLNGV GENHWSAAIL    50
KRALANDSAW HRLSEKEFAH LQTLLPKPPA HHPHYAFRFI DLFAGIGGIR 100
RGFESIGGQC VFTSEWNKHA VRTYKANHYC DPATHHFNED IRDITLSHKE 150
GVSDEAAAEH IRQHIPEHDV LLAGFPCQPF SLAGVSKKNS LGRAHGFACD 200
TQGTLFFDVV RIIDARRPAM FVLENVKNLK SHDQGKTFRI IMQTLDELGY 250
DVADAEDNGP DDPKIIDGKH FLPQHRERIV LVGFRRDLNL KADFTLRDIS 300
ECFPAQRVTL AQLLDPMVEA KYILTPVLWK YLYRYAKKHQ ARGNGFGYGM 350
VYPNNPQSVT RTLSARYYKD GAEILIDRGW DMATGEKDFD DPLNQQHRPR 400
RLTPRECARL MGFEAPGEAK FRIPVSDTQA YRQFGNSVVV PVFAAVAKLL 450
EPKIKQAVAL RQQEAQHGRR SR 472
Length:472
Mass (Da):53,465
Last modified:December 6, 2005 - v1
Checksum:i3635BFC001CF94B4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X13330 Genomic DNA. Translation: CAA31705.1.
M32307 Unassigned DNA. Translation: AAA03723.1.
U00096 Genomic DNA. Translation: AAC75027.1.
AP009048 Genomic DNA. Translation: BAA15788.1.
PIRiA37754. JS0263.
RefSeqiNP_416470.1. NC_000913.3.
YP_490214.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75027; AAC75027; b1961.
BAA15788; BAA15788; BAA15788.
GeneIDi12931871.
946479.
KEGGiecj:Y75_p1930.
eco:b1961.
PATRICi32119251. VBIEscCol129921_2040.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X13330 Genomic DNA. Translation: CAA31705.1 .
M32307 Unassigned DNA. Translation: AAA03723.1 .
U00096 Genomic DNA. Translation: AAC75027.1 .
AP009048 Genomic DNA. Translation: BAA15788.1 .
PIRi A37754. JS0263.
RefSeqi NP_416470.1. NC_000913.3.
YP_490214.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AED9.
SMRi P0AED9. Positions 15-466.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47858N.
IntActi P0AED9. 21 interactions.
MINTi MINT-1237420.
STRINGi 511145.b1961.

Protein family/group databases

REBASEi 2397. M.EcoKDcm.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75027 ; AAC75027 ; b1961 .
BAA15788 ; BAA15788 ; BAA15788 .
GeneIDi 12931871.
946479.
KEGGi ecj:Y75_p1930.
eco:b1961.
PATRICi 32119251. VBIEscCol129921_2040.

Organism-specific databases

EchoBASEi EB0207.
EcoGenei EG10211. dcm.

Phylogenomic databases

eggNOGi COG0270.
HOGENOMi HOG000225506.
KOi K00558.
OMAi IDGRHFL.
OrthoDBi EOG6PZXGV.
PhylomeDBi P0AED9.

Enzyme and pathway databases

BioCyci EcoCyc:EG10211-MONOMER.
ECOL316407:JW1944-MONOMER.
MetaCyc:EG10211-MONOMER.

Miscellaneous databases

PROi P0AED9.

Gene expression databases

Genevestigatori P0AED9.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the dcm locus of Escherichia coli K12."
    Hanck T., Gerwin N., Fritz H.-J.
    Nucleic Acids Res. 17:5844-5844(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "A gene required for very short patch repair in Escherichia coli is adjacent to the DNA cytosine methylase gene."
    Sohail A., Lieb M., Dar M., Bhagwat A.S.
    J. Bacteriol. 172:4214-4221(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiDCM_ECOLI
AccessioniPrimary (citable) accession number: P0AED9
Secondary accession number(s): P11876
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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