Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P0AED9 (DCM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-cytosine methyltransferase

EC=2.1.1.37
Alternative name(s):
M.EcoDcm
Gene names
Name:dcm
Synonyms:mec
Ordered Locus Names:b1961, JW1944
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

aceFP069592EBI-548525,EBI-542707

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 472472DNA-cytosine methyltransferase
PRO_0000087913

Regions

Domain87 – 457371SAM-dependent MTase C5-type

Sites

Active site1771 By similarity

Sequences

Sequence LengthMass (Da)Tools
P0AED9 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 3635BFC001CF94B4

FASTA47253,465
        10         20         30         40         50         60 
MQENISVTDS YSTGNAAQAM LEKLLQIYDV KTLVAQLNGV GENHWSAAIL KRALANDSAW 

        70         80         90        100        110        120 
HRLSEKEFAH LQTLLPKPPA HHPHYAFRFI DLFAGIGGIR RGFESIGGQC VFTSEWNKHA 

       130        140        150        160        170        180 
VRTYKANHYC DPATHHFNED IRDITLSHKE GVSDEAAAEH IRQHIPEHDV LLAGFPCQPF 

       190        200        210        220        230        240 
SLAGVSKKNS LGRAHGFACD TQGTLFFDVV RIIDARRPAM FVLENVKNLK SHDQGKTFRI 

       250        260        270        280        290        300 
IMQTLDELGY DVADAEDNGP DDPKIIDGKH FLPQHRERIV LVGFRRDLNL KADFTLRDIS 

       310        320        330        340        350        360 
ECFPAQRVTL AQLLDPMVEA KYILTPVLWK YLYRYAKKHQ ARGNGFGYGM VYPNNPQSVT 

       370        380        390        400        410        420 
RTLSARYYKD GAEILIDRGW DMATGEKDFD DPLNQQHRPR RLTPRECARL MGFEAPGEAK 

       430        440        450        460        470 
FRIPVSDTQA YRQFGNSVVV PVFAAVAKLL EPKIKQAVAL RQQEAQHGRR SR 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the dcm locus of Escherichia coli K12."
Hanck T., Gerwin N., Fritz H.-J.
Nucleic Acids Res. 17:5844-5844(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A gene required for very short patch repair in Escherichia coli is adjacent to the DNA cytosine methylase gene."
Sohail A., Lieb M., Dar M., Bhagwat A.S.
J. Bacteriol. 172:4214-4221(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X13330 Genomic DNA. Translation: CAA31705.1.
M32307 Unassigned DNA. Translation: AAA03723.1.
U00096 Genomic DNA. Translation: AAC75027.1.
AP009048 Genomic DNA. Translation: BAA15788.1.
PIRJS0263. A37754.
RefSeqNP_416470.1. NC_000913.3.
YP_490214.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0AED9.
SMRP0AED9. Positions 15-466.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-47858N.
IntActP0AED9. 21 interactions.
MINTMINT-1237420.
STRING511145.b1961.

Protein family/group databases

REBASE2397. M.EcoKDcm.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75027; AAC75027; b1961.
BAA15788; BAA15788; BAA15788.
GeneID12931871.
946479.
KEGGecj:Y75_p1930.
eco:b1961.
PATRIC32119251. VBIEscCol129921_2040.

Organism-specific databases

EchoBASEEB0207.
EcoGeneEG10211. dcm.

Phylogenomic databases

eggNOGCOG0270.
HOGENOMHOG000225506.
KOK00558.
OMANWYCDPQ.
OrthoDBEOG6PZXGV.
PhylomeDBP0AED9.
ProtClustDBPRK10458.

Enzyme and pathway databases

BioCycEcoCyc:EG10211-MONOMER.
ECOL316407:JW1944-MONOMER.
MetaCyc:EG10211-MONOMER.

Gene expression databases

GenevestigatorP0AED9.

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0AED9.

Entry information

Entry nameDCM_ECOLI
AccessionPrimary (citable) accession number: P0AED9
Secondary accession number(s): P11876
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene