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P0AED9

- DCM_ECOLI

UniProt

P0AED9 - DCM_ECOLI

Protein

DNA-cytosine methyltransferase

Gene

dcm

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei177 – 1771PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: EcoCyc
    2. DNA binding Source: InterPro
    3. protein binding Source: IntAct

    GO - Biological processi

    1. C-5 methylation of cytosine Source: GOC
    2. DNA methylation Source: EcoCyc
    3. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10211-MONOMER.
    ECOL316407:JW1944-MONOMER.
    MetaCyc:EG10211-MONOMER.

    Protein family/group databases

    REBASEi2397. M.EcoKDcm.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-cytosine methyltransferase (EC:2.1.1.37)
    Alternative name(s):
    M.EcoDcm
    Gene namesi
    Name:dcm
    Synonyms:mec
    Ordered Locus Names:b1961, JW1944
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10211. dcm.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 472472DNA-cytosine methyltransferasePRO_0000087913Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP0AED9.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    aceFP069592EBI-548525,EBI-542707

    Protein-protein interaction databases

    DIPiDIP-47858N.
    IntActiP0AED9. 21 interactions.
    MINTiMINT-1237420.
    STRINGi511145.b1961.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AED9.
    SMRiP0AED9. Positions 15-466.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini87 – 457371SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0270.
    HOGENOMiHOG000225506.
    KOiK00558.
    OMAiIDGRHFL.
    OrthoDBiEOG6PZXGV.
    PhylomeDBiP0AED9.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AED9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQENISVTDS YSTGNAAQAM LEKLLQIYDV KTLVAQLNGV GENHWSAAIL    50
    KRALANDSAW HRLSEKEFAH LQTLLPKPPA HHPHYAFRFI DLFAGIGGIR 100
    RGFESIGGQC VFTSEWNKHA VRTYKANHYC DPATHHFNED IRDITLSHKE 150
    GVSDEAAAEH IRQHIPEHDV LLAGFPCQPF SLAGVSKKNS LGRAHGFACD 200
    TQGTLFFDVV RIIDARRPAM FVLENVKNLK SHDQGKTFRI IMQTLDELGY 250
    DVADAEDNGP DDPKIIDGKH FLPQHRERIV LVGFRRDLNL KADFTLRDIS 300
    ECFPAQRVTL AQLLDPMVEA KYILTPVLWK YLYRYAKKHQ ARGNGFGYGM 350
    VYPNNPQSVT RTLSARYYKD GAEILIDRGW DMATGEKDFD DPLNQQHRPR 400
    RLTPRECARL MGFEAPGEAK FRIPVSDTQA YRQFGNSVVV PVFAAVAKLL 450
    EPKIKQAVAL RQQEAQHGRR SR 472
    Length:472
    Mass (Da):53,465
    Last modified:December 6, 2005 - v1
    Checksum:i3635BFC001CF94B4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13330 Genomic DNA. Translation: CAA31705.1.
    M32307 Unassigned DNA. Translation: AAA03723.1.
    U00096 Genomic DNA. Translation: AAC75027.1.
    AP009048 Genomic DNA. Translation: BAA15788.1.
    PIRiA37754. JS0263.
    RefSeqiNP_416470.1. NC_000913.3.
    YP_490214.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75027; AAC75027; b1961.
    BAA15788; BAA15788; BAA15788.
    GeneIDi12931871.
    946479.
    KEGGiecj:Y75_p1930.
    eco:b1961.
    PATRICi32119251. VBIEscCol129921_2040.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13330 Genomic DNA. Translation: CAA31705.1 .
    M32307 Unassigned DNA. Translation: AAA03723.1 .
    U00096 Genomic DNA. Translation: AAC75027.1 .
    AP009048 Genomic DNA. Translation: BAA15788.1 .
    PIRi A37754. JS0263.
    RefSeqi NP_416470.1. NC_000913.3.
    YP_490214.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AED9.
    SMRi P0AED9. Positions 15-466.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47858N.
    IntActi P0AED9. 21 interactions.
    MINTi MINT-1237420.
    STRINGi 511145.b1961.

    Protein family/group databases

    REBASEi 2397. M.EcoKDcm.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75027 ; AAC75027 ; b1961 .
    BAA15788 ; BAA15788 ; BAA15788 .
    GeneIDi 12931871.
    946479.
    KEGGi ecj:Y75_p1930.
    eco:b1961.
    PATRICi 32119251. VBIEscCol129921_2040.

    Organism-specific databases

    EchoBASEi EB0207.
    EcoGenei EG10211. dcm.

    Phylogenomic databases

    eggNOGi COG0270.
    HOGENOMi HOG000225506.
    KOi K00558.
    OMAi IDGRHFL.
    OrthoDBi EOG6PZXGV.
    PhylomeDBi P0AED9.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10211-MONOMER.
    ECOL316407:JW1944-MONOMER.
    MetaCyc:EG10211-MONOMER.

    Miscellaneous databases

    PROi P0AED9.

    Gene expression databases

    Genevestigatori P0AED9.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the dcm locus of Escherichia coli K12."
      Hanck T., Gerwin N., Fritz H.-J.
      Nucleic Acids Res. 17:5844-5844(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "A gene required for very short patch repair in Escherichia coli is adjacent to the DNA cytosine methylase gene."
      Sohail A., Lieb M., Dar M., Bhagwat A.S.
      J. Bacteriol. 172:4214-4221(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiDCM_ECOLI
    AccessioniPrimary (citable) accession number: P0AED9
    Secondary accession number(s): P11876
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3