Reviewed, UniProtKB/Swiss-Prot P0AED9 (DCM_ECOLI)

Names and origin

Protein names

Recommended name:
    DNA-cytosine methyltransferase
    EC=2.1.1.37
Alternative name(s):
    M.EcoDcm

Gene names

Name:	dcm
Synonyms:	mec
Ordered Locus Names:	b1961, JW1944

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
aceF	P06959	1	EBI-548525,EBI-542707

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	DNA-cytosine methyltransferase		PRO_0000087913
Sites
	Active site	177	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AED9-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: 3635BFC001CF94B4	FASTA	472	53,465
	10 20 30 40 50 60 MQENISVTDS YSTGNAAQAM LEKLLQIYDV KTLVAQLNGV GENHWSAAIL KRALANDSAW 70 80 90 100 110 120 HRLSEKEFAH LQTLLPKPPA HHPHYAFRFI DLFAGIGGIR RGFESIGGQC VFTSEWNKHA 130 140 150 160 170 180 VRTYKANHYC DPATHHFNED IRDITLSHKE GVSDEAAAEH IRQHIPEHDV LLAGFPCQPF 190 200 210 220 230 240 SLAGVSKKNS LGRAHGFACD TQGTLFFDVV RIIDARRPAM FVLENVKNLK SHDQGKTFRI 250 260 270 280 290 300 IMQTLDELGY DVADAEDNGP DDPKIIDGKH FLPQHRERIV LVGFRRDLNL KADFTLRDIS 310 320 330 340 350 360 ECFPAQRVTL AQLLDPMVEA KYILTPVLWK YLYRYAKKHQ ARGNGFGYGM VYPNNPQSVT 370 380 390 400 410 420 RTLSARYYKD GAEILIDRGW DMATGEKDFD DPLNQQHRPR RLTPRECARL MGFEAPGEAK 430 440 450 460 470 FRIPVSDTQA YRQFGNSVVV PVFAAVAKLL EPKIKQAVAL RQQEAQHGRR SR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the dcm locus of Escherichia coli K12." Hanck T., Gerwin N., Fritz H.-J. Nucleic Acids Res. 17:5844-5844(1989) [PubMed: 2527357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A gene required for very short patch repair in Escherichia coli is adjacent to the DNA cytosine methylase gene." Sohail A., Lieb M., Dar M., Bhagwat A.S. J. Bacteriol. 172:4214-4221(1990) [PubMed: 2198248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X13330 Genomic DNA. Translation: CAA31705.1. M32307 Unassigned DNA. Translation: AAA03723.1. U00096 Genomic DNA. Translation: AAC75027.1. AP009048 Genomic DNA. Translation: BAA15788.1.
PIR	JS0263. A37754.
RefSeq	AP_002572.1. NP_416470.1.
3D structure databases
HSSP	HSSP built from PDB template 1FJX based on UniProtKB P05102.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AED9. 20 interactions.
Protein family/group databases
REBASE	2397. M.EcoKDcm.
Genome annotation databases
GeneID	946479.
GenomeReviews	Gene locus JW1944 in contig AP009048_GR. Gene locus b1961 in contig U00096_GR.
KEGG	ecj:JW1944. eco:b1961.
Organism-specific databases
EchoBASE	EB0207.
EcoGene	EG10211. dcm.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AED9.
OMA	P0AED9. NALGRPH.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10211-MON.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCM_ECOLI

Accession

Primary (citable) accession number: P0AED9
Secondary accession number(s): P11876

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)