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Protein

Universal stress protein A

Gene

uspA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for resistance to DNA-damaging agents.

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG11390-MONOMER.
ECOL316407:JW3462-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Universal stress protein A
Gene namesi
Name:uspA
Ordered Locus Names:b3495, JW3462
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11390. uspA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved4 Publications
Chaini2 – 144143Universal stress protein APRO_0000147396Add
BLAST

Post-translational modificationi

Phosphorylated on serine or threonine in response to stasis.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP0AED0.
PaxDbiP0AED0.
PRIDEiP0AED0.

2D gel databases

SWISS-2DPAGEP0AED0.

Expressioni

Inductioni

During growth inhibition caused by the exhaustion of any of a variety of nutrients (carbon, nitrogen, phosphate, sulfate, required amino acid) or by the presence of a variety of toxic agents. Positively regulated by guanosine 3',5'-bisphosphate (ppGpp) and by a RecA/FtsK-dependent regulatory pathway. Negatively regulated by FadR. Also regulated by CspC and CspE.

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-552843,EBI-552843

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4262517. 2 interactions.
DIPiDIP-36230N.
IntActiP0AED0. 9 interactions.
MINTiMINT-1291607.
STRINGi511145.b3495.

Structurei

3D structure databases

ProteinModelPortaliP0AED0.
SMRiP0AED0. Positions 3-141.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the universal stress protein A family.Curated

Phylogenomic databases

eggNOGiCOG0589. LUCA.
HOGENOMiHOG000238899.
InParanoidiP0AED0.
KOiK06149.
OMAiSARQLMN.
PhylomeDBiP0AED0.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR006015. Universal_stress_UspA.
IPR006016. UspA.
[Graphical view]
PfamiPF00582. Usp. 1 hit.
[Graphical view]
PIRSFiPIRSF006276. UspA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AED0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYKHILIAV DLSPESKVLV EKAVSMARPY NAKVSLIHVD VNYSDLYTGL
60 70 80 90 100
IDVNLGDMQK RISEETHHAL TELSTNAGYP ITETLSGSGD LGQVLVDAIK
110 120 130 140
KYDMDLVVCG HHQDFWSKLM SSARQLINTV HVDMLIVPLR DEEE
Length:144
Mass (Da):16,066
Last modified:January 23, 2007 - v2
Checksum:iEE3BF2141E44D826
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti140 – 1401R → A in CAA47884 (PubMed:1453957).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67639 Genomic DNA. Translation: CAA47884.1.
U00039 Genomic DNA. Translation: AAB18471.1.
U00096 Genomic DNA. Translation: AAC76520.1.
AP009048 Genomic DNA. Translation: BAE77799.1.
PIRiS47715.
RefSeqiNP_417952.1. NC_000913.3.
WP_000323571.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76520; AAC76520; b3495.
BAE77799; BAE77799; BAE77799.
GeneIDi948007.
KEGGiecj:JW3462.
eco:b3495.
PATRICi32122440. VBIEscCol129921_3597.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67639 Genomic DNA. Translation: CAA47884.1.
U00039 Genomic DNA. Translation: AAB18471.1.
U00096 Genomic DNA. Translation: AAC76520.1.
AP009048 Genomic DNA. Translation: BAE77799.1.
PIRiS47715.
RefSeqiNP_417952.1. NC_000913.3.
WP_000323571.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AED0.
SMRiP0AED0. Positions 3-141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262517. 2 interactions.
DIPiDIP-36230N.
IntActiP0AED0. 9 interactions.
MINTiMINT-1291607.
STRINGi511145.b3495.

2D gel databases

SWISS-2DPAGEP0AED0.

Proteomic databases

EPDiP0AED0.
PaxDbiP0AED0.
PRIDEiP0AED0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76520; AAC76520; b3495.
BAE77799; BAE77799; BAE77799.
GeneIDi948007.
KEGGiecj:JW3462.
eco:b3495.
PATRICi32122440. VBIEscCol129921_3597.

Organism-specific databases

EchoBASEiEB1363.
EcoGeneiEG11390. uspA.

Phylogenomic databases

eggNOGiCOG0589. LUCA.
HOGENOMiHOG000238899.
InParanoidiP0AED0.
KOiK06149.
OMAiSARQLMN.
PhylomeDBiP0AED0.

Enzyme and pathway databases

BioCyciEcoCyc:EG11390-MONOMER.
ECOL316407:JW3462-MONOMER.

Miscellaneous databases

PROiP0AED0.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR006015. Universal_stress_UspA.
IPR006016. UspA.
[Graphical view]
PfamiPF00582. Usp. 1 hit.
[Graphical view]
PIRSFiPIRSF006276. UspA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUSPA_ECOLI
AccessioniPrimary (citable) accession number: P0AED0
Secondary accession number(s): P28242, Q2M7F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.