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P0AEC9 (DCUS_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Sensor histidine kinase DcuS
EC=2.7.13.3
Gene names
Name:dcuS
Ordered Locus Names:Z5727, ECs5107
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length543 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Member of the two-component regulatory system DcuR/DcuS. Involved in the C4-dicarboxylate-stimulated regulation of the genes encoding the anaerobic fumarate respiratory system (frdABCD; nuoAN; dcuB; dcuC; sdhCDAB; etc.). Weakly regulates the aerobic C4-dicarboxylate transporter dctA. Activates DcuR by phosphorylation By similarity.

Catalytic activity

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Subunit structure

Homodimer By similarity.

Subcellular location

Cell inner membrane; Multi-pass membrane protein By similarity.

Domain

The periplasmic domain is involved in C4-dicarboxylate binding and sensing. The structural disorder in the cytoplasmic PAS domain has an important role in signal transduction to the kinase domain and may be the decisive structural feature that characterizes the activated kinase By similarity.

Post-translational modification

Autophosphorylated. The phosphoryl group is rapidely transferred to DcuR By similarity.

Sequence similarities

Contains 1 histidine kinase domain.

Contains 1 PAS (PER-ARNT-SIM) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 543543Sensor histidine kinase DcuS
PRO_0000074751

Regions

Topological domain1 – 2020Cytoplasmic Potential
Transmembrane21 – 4121Helical; Potential
Topological domain42 – 181140Periplasmic Potential
Transmembrane182 – 20221Helical; Potential
Topological domain203 – 543341Cytoplasmic Potential
Domain212 – 323112PAS
Domain346 – 538193Histidine kinase
Region140 – 1423C4-dicarboxylate binding By similarity

Sites

Binding site1071C4-dicarboxylate By similarity
Binding site1101C4-dicarboxylate By similarity
Binding site1211C4-dicarboxylate; via amide nitrogen and carbonyl oxygen By similarity
Binding site1471C4-dicarboxylate By similarity

Amino acid modifications

Modified residue3491Phosphohistidine; by autocatalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
P0AEC9 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 653C369344CBA238

FASTA54360,551
        10         20         30         40         50         60 
MRHSLPYRML RKRPMKLSTT VILMVSAVLF SVLLVVHLIY FSQISDMTRD GLANKALAVA 

        70         80         90        100        110        120 
RTLADSPEIR QGLQKKPQES GIQAIAEAVR KRNDLLFIVV TDMQSLRYSH PEAQRIGQPF 

       130        140        150        160        170        180 
KGDDILKALN GEENVAINRG FLAQALRVFT PIYDENHKQI GVVAIGLELS RVTQQINDSR 

       190        200        210        220        230        240 
WSIIWSVLFG MLVGLIGTCI LVKVLKKILF GLEPYEISTL FEQRQAMLQS IKEGVVAVDD 

       250        260        270        280        290        300 
RGEVTLINDA AQELLNYRKS QDDEKLSTLS HSWSQVVDVS EVLRDGTPRR DEEITIKDRL 

       310        320        330        340        350        360 
LLINTVPVRS NGVIIGAIST FRDKTEVRKL MQRLDGLVNY ADALRERSHE FMNKLHVILG 

       370        380        390        400        410        420 
LLHLKSYKQL EDYILKTANN YQEEIGSLLG KIKSPVIAGF LISKINRATD LGHTLILNSE 

       430        440        450        460        470        480 
SQLPDSGSED QVATLITTLG NLIENALEAL GPEPGGEISV TLHYRHGWLH CEVNDDGPGI 

       490        500        510        520        530        540 
APDKIDHIFD KGVSTKGSER GVGLALVKQQ VENLGGSIAV ESEPGIFTQF FVQIPWDGER 


SNR

« Hide

References

[1]"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7."
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. expand/collapse author list , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.
Nature 409:529-533(2001) [PubMed: 11206551] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. expand/collapse author list , Kuhara S., Shiba T., Hattori M., Shinagawa H.
DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005174 Genomic DNA. Translation: AAG59324.1.
BA000007 Genomic DNA. Translation: BAB38530.1.
PIRC91267.
H86107.
RefSeqNP_290758.1. NC_002655.2.
NP_313134.1. NC_002695.1.

3D structure databases

ProteinModelPortalP0AEC9.
SMRP0AEC9. Positions 45-179, 211-540.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000026461; EBESCP00000025354; EBESCG00000025514.
EBESCT00000057843; EBESCP00000055671; EBESCG00000056891.
GeneID914212.
959999.
GenomeReviewsGene locus Z5727 in contig AE005174_GR.
Gene locus ECs5107 in contig BA000007_GR.
KEGGece:Z5727.
ecs:ECs5107.
PATRIC18359825. VBIEscCol44059_5049.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000008665.
HOGENOMHBG537380.
OMATMLQSVK.
ProtClustDBPRK11086.

Enzyme and pathway databases

BioCycECOL83334:ECS5107-MONOMER.

Family and domain databases

InterProIPR003594. ATPase-like_ATP-bd.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR016120. Sig_transdc_His_kin_SpoOB.
IPR005467. Sig_transdc_His_kinase_core.
IPR016121. Spore_sensor_kin_SpoOB_hlx.
[Graphical view]
Gene3DG3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
G3DSA:1.10.287.130. Sensor_kinase_SpoOB-type_hlx. 1 hit.
KOK07701.
PfamPF02518. HATPase_c. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view]
PRINTSPR00344. BCTRLSENSOR.
SMARTSM00387. HATPase_c. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMSSF55874. ATP_bd_ATPase. 1 hit.
SSF55890. Sensor_kinase_SpoOB-type. 1 hit.
PROSITEPS50109. HIS_KIN. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDCUS_ECO57
AccessionPrimary (citable) accession number: P0AEC9
Secondary accession number(s): P39272, P76795
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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