Sensor protein dcuS - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0AEC8 (DCUS_ECOLI)

Last modified June 16, 2009. Version 41. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Sensor protein dcuS
EC=2.7.13.3

Gene names

Name:	dcuS
Synonyms:	yjdH
Ordered Locus Names:	b4125, JW4086

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Member of the two-component regulatory system dcuR/dcuS. Involved in the C4-dicarboxylate-stimulated regulation of the genes encoding the anaerobic fumarate respiratory system (frdABCD; nuoAN; dcuB; dcuC; sdhCDAB; etc.). Weakly regulates the aerobic C4-dicarboxylate transporter dctA. Activates dcuR by phosphorylation.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Enzyme regulation	Autophosphorylation is stimulated by the presence of fumarate or succinate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Post-translational modification	Autophosphorylated. The phosphoryl group is rapidely transferred to dcuR.
Miscellaneous	The region encompassing approximately residues 42 to 181 has been shown to be periplasmic, however exactly which residues are periplasmic is not clear.
Sequence similarities	Contains 1 histidine kinase domain. Contains 1 PAS (PER-ARNT-SIM) domain.

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Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
sdhA	P0AC41	1	EBI-1134683,EBI-371263

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 543

543

Sensor protein dcuS

PRO_0000074750

Regions

Topological domain

1 – 20

Cytoplasmic Potential

Transmembrane

21 – 41

Potential

Topological domain

42 – 181

140

Periplasmic Potential

Transmembrane

182 – 202

Potential

Topological domain

203 – 543

341

Cytoplasmic Potential

Domain

220 – 291

PAS

Domain

346 – 538

193

Histidine kinase

Amino acid modifications

Modified residue

349

Phosphohistidine; by autocatalysis By similarity

Secondary structure

543

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P0AEC8-1 [UniParc].

Last modified December 6, 2005. Version 1.
Checksum: 653C369344CBA238
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FASTA

543

60,551

        10         20         30         40         50         60 
MRHSLPYRML RKRPMKLSTT VILMVSAVLF SVLLVVHLIY FSQISDMTRD GLANKALAVA 

        70         80         90        100        110        120 
RTLADSPEIR QGLQKKPQES GIQAIAEAVR KRNDLLFIVV TDMQSLRYSH PEAQRIGQPF 

       130        140        150        160        170        180 
KGDDILKALN GEENVAINRG FLAQALRVFT PIYDENHKQI GVVAIGLELS RVTQQINDSR 

       190        200        210        220        230        240 
WSIIWSVLFG MLVGLIGTCI LVKVLKKILF GLEPYEISTL FEQRQAMLQS IKEGVVAVDD 

       250        260        270        280        290        300 
RGEVTLINDA AQELLNYRKS QDDEKLSTLS HSWSQVVDVS EVLRDGTPRR DEEITIKDRL 

       310        320        330        340        350        360 
LLINTVPVRS NGVIIGAIST FRDKTEVRKL MQRLDGLVNY ADALRERSHE FMNKLHVILG 

       370        380        390        400        410        420 
LLHLKSYKQL EDYILKTANN YQEEIGSLLG KIKSPVIAGF LISKINRATD LGHTLILNSE 

       430        440        450        460        470        480 
SQLPDSGSED QVATLITTLG NLIENALEAL GPEPGGEISV TLHYRHGWLH CEVNDDGPGI 

       490        500        510        520        530        540 
APDKIDHIFD KGVSTKGSER GVGLALVKQQ VENLGGSIAV ESEPGIFTQF FVQIPWDGER 


SNR

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system." Zientz E., Bongaerts J., Unden G. J. Bacteriol. 180:5421-5425(1998) [PubMed: 9765574] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[5]	"Identification and characterization of a two-component sensor-kinase and response-regulator system (DcuS-DcuR) controlling gene expression in response to C4-dicarboxylates in Escherichia coli." Golby P., Davies S., Kelly D.J., Guest J.R., Andrews S.C. J. Bacteriol. 181:1238-1248(1999) [PubMed: 9973351] [Abstract] Cited for: CHARACTERIZATION, TOPOLOGY. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[6]	"Function of DcuS from Escherichia coli as a fumarate-stimulated histidine protein kinase in vitro." Janausch I.G., Garcia-Moreno I., Unden G. J. Biol. Chem. 277:39809-39814(2002) [PubMed: 12167640] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / AN387.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U14003 Genomic DNA. Translation: AAA97025.1.
U00096 Genomic DNA. Translation: AAC77086.1.
AP009048 Genomic DNA. Translation: BAE78127.1.

PIR

D65222.

RefSeq

AP_004626.1.
NP_418549.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OJG	NMR	-	A	45-180	[»]
2W0N	NMR	-	A	211-325	[»]
3BY8	X-ray	1.45	A	42-181	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0AEC8. 1 interaction.

Genome annotation databases

GeneID

948639.

GenomeReviews

Gene locus JW4086 in contig AP009048_GR.
Gene locus b4125 in contig U00096_GR.

KEGG

ecj:JW4086.
eco:b4125.

Organism-specific databases

EchoBASE

EB2358.

EcoGene

EG12465. dcuS.

CMR

Search...

Phylogenomic databases

HOGENOM

P0AEC8.

OMA

P0AEC8. RNTMLQS.

Enzyme and pathway databases

BioCyc

EcoCyc:DCUS-MON.

Family and domain databases

InterPro

IPR003594. ATP_bd_ATPase.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR005467. Sig_transdc_His_kinase_core.
[Graphical view]

Gene3D

G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.

Pfam

PF02518. HATPase_c. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view]

PRINTS

PR00344. BCTRLSENSOR.

SMART

SM00387. HATPase_c. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00229. sensory_box. 1 hit.

PROSITE

PS50109. HIS_KIN. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

DCUS_ECOLI

Accession

Primary (citable) accession number: P0AEC8
Secondary accession number(s): P39272, P76795, Q2M6H9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents