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Protein

Sensor histidine kinase DcuS

Gene

dcuS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system DcuR/DcuS. Involved in the C4-dicarboxylate-stimulated regulation of the genes encoding the anaerobic fumarate respiratory system (frdABCD; nuoAN; dcuB; dcuC; sdhCDAB; etc.). Weakly regulates the aerobic C4-dicarboxylate transporter dctA. Activates DcuR by phosphorylation.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Enzyme regulationi

Autophosphorylation is stimulated by the presence of C4-dicarboxylates such as fumarate, succinate, malate, and tartrate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107C4-dicarboxylate1
Binding sitei110C4-dicarboxylate1
Binding sitei121C4-dicarboxylate; via amide nitrogen and carbonyl oxygen1
Binding sitei147C4-dicarboxylate1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • phosphorelay sensor kinase activity Source: EcoliWiki
  • protein histidine kinase activity Source: EcoliWiki
  • signal transducer activity Source: EcoliWiki

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoliWiki
  • protein homooligomerization Source: EcoCyc
  • protein phosphorylation Source: EcoliWiki
  • regulation of transcription, DNA-templated Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:DCUS-MONOMER.
ECOL316407:JW4086-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase DcuS (EC:2.7.13.3)
Alternative name(s):
Fumarate sensor
Gene namesi
Name:dcuS
Synonyms:yjdH
Ordered Locus Names:b4125, JW4086
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12465. dcuS.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20CytoplasmicSequence analysisAdd BLAST20
Transmembranei21 – 41HelicalSequence analysisAdd BLAST21
Topological domaini42 – 181PeriplasmicSequence analysisAdd BLAST140
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
Topological domaini203 – 543CytoplasmicSequence analysisAdd BLAST341

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • integral component of plasma membrane Source: EcoCyc
  • intracellular Source: GOC
  • plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi107R → A: Abolishes the stimulation by fumarate to the same extent as complete deletion of the dcuS gene. 1 Publication1
Mutagenesisi110H → A: Abolishes the stimulation by fumarate to the same extent as complete deletion of the dcuS gene. 1 Publication1
Mutagenesisi147R → A: Abolishes the stimulation by fumarate to the same extent as complete deletion of the dcuS gene. 1 Publication1
Mutagenesisi248N → A, D or G: Causes constitutive active state of the kinase, leading to constitutive expression of a target gene, without addition of C4-dicarboxylates. 1 Publication1
Mutagenesisi304N → D: Causes constitutive active state of the kinase, leading to constitutive expression of a target gene, without addition of C4-dicarboxylates. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000747501 – 543Sensor histidine kinase DcuSAdd BLAST543

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei349Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated. The phosphoryl group is rapidly transferred to DcuR.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AEC8.
PRIDEiP0AEC8.

Interactioni

Subunit structurei

Homodimer.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself8EBI-1134683,EBI-1134683

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4261981. 12 interactors.
DIPiDIP-9414N.
IntActiP0AEC8. 3 interactors.
STRINGi511145.b4125.

Structurei

Secondary structure

1543
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi47 – 64Combined sources18
Helixi67 – 72Combined sources6
Helixi77 – 79Combined sources3
Helixi82 – 92Combined sources11
Beta strandi96 – 102Combined sources7
Beta strandi106 – 108Combined sources3
Helixi113 – 115Combined sources3
Helixi123 – 125Combined sources3
Helixi127 – 130Combined sources4
Beta strandi134 – 138Combined sources5
Beta strandi140 – 143Combined sources4
Beta strandi145 – 153Combined sources9
Beta strandi155 – 157Combined sources3
Beta strandi159 – 168Combined sources10
Helixi169 – 177Combined sources9
Helixi214 – 217Combined sources4
Helixi221 – 229Combined sources9
Beta strandi236 – 239Combined sources4
Turni240 – 242Combined sources3
Helixi249 – 255Combined sources7
Turni259 – 263Combined sources5
Helixi277 – 284Combined sources8
Beta strandi294 – 298Combined sources5
Beta strandi317 – 319Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OJGNMR-A45-180[»]
2W0NNMR-A211-325[»]
3BY8X-ray1.45A42-181[»]
ProteinModelPortaliP0AEC8.
SMRiP0AEC8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEC8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini212 – 323PASPROSITE-ProRule annotationAdd BLAST112
Domaini346 – 538Histidine kinasePROSITE-ProRule annotationAdd BLAST193

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni140 – 142C4-dicarboxylate binding3

Domaini

The periplasmic domain is involved in C4-dicarboxylate binding and sensing. The structural disorder in the cytoplasmic PAS domain has an important role in signal transduction to the kinase domain and may be the decisive structural feature that characterizes the activated kinase.3 Publications

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CEQ. Bacteria.
COG3290. LUCA.
HOGENOMiHOG000241936.
InParanoidiP0AEC8.
KOiK07701.
OMAiHYQNGWL.
PhylomeDBiP0AEC8.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR033463. sCache_3.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR016120. Sig_transdc_His_kin_SpoOB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00989. PAS. 1 hit.
PF17203. sCache_3_2. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF55890. SSF55890. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEC8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRHSLPYRML RKRPMKLSTT VILMVSAVLF SVLLVVHLIY FSQISDMTRD
60 70 80 90 100
GLANKALAVA RTLADSPEIR QGLQKKPQES GIQAIAEAVR KRNDLLFIVV
110 120 130 140 150
TDMQSLRYSH PEAQRIGQPF KGDDILKALN GEENVAINRG FLAQALRVFT
160 170 180 190 200
PIYDENHKQI GVVAIGLELS RVTQQINDSR WSIIWSVLFG MLVGLIGTCI
210 220 230 240 250
LVKVLKKILF GLEPYEISTL FEQRQAMLQS IKEGVVAVDD RGEVTLINDA
260 270 280 290 300
AQELLNYRKS QDDEKLSTLS HSWSQVVDVS EVLRDGTPRR DEEITIKDRL
310 320 330 340 350
LLINTVPVRS NGVIIGAIST FRDKTEVRKL MQRLDGLVNY ADALRERSHE
360 370 380 390 400
FMNKLHVILG LLHLKSYKQL EDYILKTANN YQEEIGSLLG KIKSPVIAGF
410 420 430 440 450
LISKINRATD LGHTLILNSE SQLPDSGSED QVATLITTLG NLIENALEAL
460 470 480 490 500
GPEPGGEISV TLHYRHGWLH CEVNDDGPGI APDKIDHIFD KGVSTKGSER
510 520 530 540
GVGLALVKQQ VENLGGSIAV ESEPGIFTQF FVQIPWDGER SNR
Length:543
Mass (Da):60,551
Last modified:December 6, 2005 - v1
Checksum:i653C369344CBA238
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97025.1.
U00096 Genomic DNA. Translation: AAC77086.1.
AP009048 Genomic DNA. Translation: BAE78127.1.
PIRiD65222.
RefSeqiNP_418549.1. NC_000913.3.
WP_001216477.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77086; AAC77086; b4125.
BAE78127; BAE78127; BAE78127.
GeneIDi948639.
KEGGiecj:JW4086.
eco:b4125.
PATRICi32123815. VBIEscCol129921_4256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97025.1.
U00096 Genomic DNA. Translation: AAC77086.1.
AP009048 Genomic DNA. Translation: BAE78127.1.
PIRiD65222.
RefSeqiNP_418549.1. NC_000913.3.
WP_001216477.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OJGNMR-A45-180[»]
2W0NNMR-A211-325[»]
3BY8X-ray1.45A42-181[»]
ProteinModelPortaliP0AEC8.
SMRiP0AEC8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261981. 12 interactors.
DIPiDIP-9414N.
IntActiP0AEC8. 3 interactors.
STRINGi511145.b4125.

Proteomic databases

PaxDbiP0AEC8.
PRIDEiP0AEC8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77086; AAC77086; b4125.
BAE78127; BAE78127; BAE78127.
GeneIDi948639.
KEGGiecj:JW4086.
eco:b4125.
PATRICi32123815. VBIEscCol129921_4256.

Organism-specific databases

EchoBASEiEB2358.
EcoGeneiEG12465. dcuS.

Phylogenomic databases

eggNOGiENOG4105CEQ. Bacteria.
COG3290. LUCA.
HOGENOMiHOG000241936.
InParanoidiP0AEC8.
KOiK07701.
OMAiHYQNGWL.
PhylomeDBiP0AEC8.

Enzyme and pathway databases

BioCyciEcoCyc:DCUS-MONOMER.
ECOL316407:JW4086-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

EvolutionaryTraceiP0AEC8.
PROiP0AEC8.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR033463. sCache_3.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR016120. Sig_transdc_His_kin_SpoOB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00989. PAS. 1 hit.
PF17203. sCache_3_2. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF55890. SSF55890. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCUS_ECOLI
AccessioniPrimary (citable) accession number: P0AEC8
Secondary accession number(s): P39272, P76795, Q2M6H9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The region encompassing approximately residues 42 to 181 has been shown to be periplasmic, however exactly which residues are periplasmic is not clear.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.