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Protein

Signal transduction histidine-protein kinase BarA

Gene

barA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histidine phosphotransfer kinase activity Source: EcoliWiki
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • cellular response to organic substance Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoliWiki
  • protein phosphorylation Source: EcoliWiki
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to hydrogen peroxide Source: EcoliWiki
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:BARA-MONOMER.
ECOL316407:JW2757-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transduction histidine-protein kinase BarA (EC:2.7.13.3)
Gene namesi
Name:barA
Ordered Locus Names:b2786, JW2757
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11367. barA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence analysis
Transmembranei10 – 3122HelicalSequence analysisAdd
BLAST
Topological domaini32 – 176145PeriplasmicSequence analysisAdd
BLAST
Transmembranei177 – 19620HelicalSequence analysisAdd
BLAST
Topological domaini197 – 918722CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 918918Signal transduction histidine-protein kinase BarAPRO_0000074697Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei302 – 3021Phosphohistidine; by autocatalysisPROSITE-ProRule annotation
Modified residuei718 – 71814-aspartylphosphatePROSITE-ProRule annotation
Modified residuei861 – 8611PhosphohistidinePROSITE-ProRule annotation

Post-translational modificationi

Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AEC5.

Interactioni

Protein-protein interaction databases

BioGridi4262286. 16 interactions.
DIPiDIP-47932N.
IntActiP0AEC5. 4 interactions.
MINTiMINT-1307958.
STRINGi511145.b2786.

Structurei

3D structure databases

ProteinModelPortaliP0AEC5.
SMRiP0AEC5. Positions 261-521, 668-918.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini200 – 25253HAMPPROSITE-ProRule annotationAdd
BLAST
Domaini299 – 520222Histidine kinasePROSITE-ProRule annotationAdd
BLAST
Domaini669 – 785117Response regulatoryPROSITE-ProRule annotationAdd
BLAST
Domaini822 – 91897HPtPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 HPt domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG0784. LUCA.
COG2198. LUCA.
COG4999. LUCA.
HOGENOMiHOG000272329.
InParanoidiP0AEC5.
KOiK07678.
OMAiISHMVSV.
OrthoDBiEOG6G4VQG.
PhylomeDBiP0AEC5.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR019247. Histidine_kinase_BarA_N.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF09984. DUF2222. 1 hit.
PF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 2 hits.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AEC5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNYSLRARM MILILAPTVL IGLLLSIFFV VHRYNDLQRQ LEDAGASIIE
60 70 80 90 100
PLAVSTEYGM SLQNRESIGQ LISVLHRRHS DIVRAISVYD ENNRLFVTSN
110 120 130 140 150
FHLDPSSMQL GSNVPFPRQL TVTRDGDIMI LRTPIISESY SPDESPSSDA
160 170 180 190 200
KNSQNMLGYI ALELDLKSVR LQQYKEIFIS SVMMLFCIGI ALIFGWRLMR
210 220 230 240 250
DVTGPIRNMV NTVDRIRRGQ LDSRVEGFML GELDMLKNGI NSMAMSLAAY
260 270 280 290 300
HEEMQHNIDQ ATSDLRETLE QMEIQNVELD LAKKRAQEAA RIKSEFLANM
310 320 330 340 350
SHELRTPLNG VIGFTRLTLK TELTPTQRDH LNTIERSANN LLAIINDVLD
360 370 380 390 400
FSKLEAGKLI LESIPFPLRS TLDEVVTLLA HSSHDKGLEL TLNIKSDVPD
410 420 430 440 450
NVIGDPLRLQ QIITNLVGNA IKFTENGNID ILVEKRALSN TKVQIEVQIR
460 470 480 490 500
DTGIGIPERD QSRLFQAFRQ ADASISRRHG GTGLGLVITQ KLVNEMGGDI
510 520 530 540 550
SFHSQPNRGS TFWFHINLDL NPNIIIEGPS TQCLAGKRLA YVEPNSAAAQ
560 570 580 590 600
CTLDILSETP LEVVYSPTFS ALPPAHYDMM LLGIAVTFRE PLTMQHERLA
610 620 630 640 650
KAVSMTDFLM LALPCHAQVN AEKLKQDGIG ACLLKPLTPT RLLPALTEFC
660 670 680 690 700
HHKQNTLLPV TDESKLAMTV MAVDDNPANL KLIGALLEDM VQHVELCDSG
710 720 730 740 750
HQAVERAKQM PFDLILMDIQ MPDMDGIRAC ELIHQLPHQQ QTPVIAVTAH
760 770 780 790 800
AMAGQKEKLL GAGMSDYLAK PIEEERLHNL LLRYKPGSGI SSRVVTPEVN
810 820 830 840 850
EIVVNPNATL DWQLALRQAA GKTDLARDML QMLLDFLPEV RNKVEEQLVG
860 870 880 890 900
ENPEGLVDLI HKLHGSCGYS GVPRMKNLCQ LIEQQLRSGT KEEDLEPELL
910
ELLDEMDNVA REASKILG
Length:918
Mass (Da):102,453
Last modified:December 6, 2005 - v1
Checksum:iEA31D6D732F023CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10888 Genomic DNA. Translation: BAA01710.1.
U29580 Genomic DNA. Translation: AAA69296.1.
U00096 Genomic DNA. Translation: AAC75828.1.
AP009048 Genomic DNA. Translation: BAA16571.2.
PIRiS20550.
RefSeqiNP_417266.1. NC_000913.3.
WP_000186450.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75828; AAC75828; b2786.
BAA16571; BAA16571; BAA16571.
GeneIDi947255.
KEGGiecj:JW2757.
eco:b2786.
PATRICi32120988. VBIEscCol129921_2886.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10888 Genomic DNA. Translation: BAA01710.1.
U29580 Genomic DNA. Translation: AAA69296.1.
U00096 Genomic DNA. Translation: AAC75828.1.
AP009048 Genomic DNA. Translation: BAA16571.2.
PIRiS20550.
RefSeqiNP_417266.1. NC_000913.3.
WP_000186450.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AEC5.
SMRiP0AEC5. Positions 261-521, 668-918.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262286. 16 interactions.
DIPiDIP-47932N.
IntActiP0AEC5. 4 interactions.
MINTiMINT-1307958.
STRINGi511145.b2786.

Proteomic databases

PaxDbiP0AEC5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75828; AAC75828; b2786.
BAA16571; BAA16571; BAA16571.
GeneIDi947255.
KEGGiecj:JW2757.
eco:b2786.
PATRICi32120988. VBIEscCol129921_2886.

Organism-specific databases

EchoBASEiEB1341.
EcoGeneiEG11367. barA.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG0784. LUCA.
COG2198. LUCA.
COG4999. LUCA.
HOGENOMiHOG000272329.
InParanoidiP0AEC5.
KOiK07678.
OMAiISHMVSV.
OrthoDBiEOG6G4VQG.
PhylomeDBiP0AEC5.

Enzyme and pathway databases

BioCyciEcoCyc:BARA-MONOMER.
ECOL316407:JW2757-MONOMER.

Miscellaneous databases

PROiP0AEC5.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR019247. Histidine_kinase_BarA_N.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF09984. DUF2222. 1 hit.
PF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47226. SSF47226. 2 hits.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel sensor-regulator protein that belongs to the homologous family of signal-transduction proteins involved in adaptive responses in Escherichia coli."
    Nagasawa S., Tokishita S., Aiba H., Mizuno T.
    Mol. Microbiol. 6:799-807(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 106-918.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Identification of UvrY as the cognate response regulator for the BarA sensor kinase in Escherichia coli."
    Pernestig A.-K., Melefors O., Georgellis D.
    J. Biol. Chem. 276:225-231(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  6. "Regulatory circuitry of the CsrA/CsrB and BarA/UvrY systems of Escherichia coli."
    Suzuki K., Wang X., Weilbacher T., Pernestig A.-K., Melefors O., Georgellis D., Babitzke P., Romeo T.
    J. Bacteriol. 184:5130-5140(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  7. "The Escherichia coli BarA-UvrY two-component system is needed for efficient switching between glycolytic and gluconeogenic carbon sources."
    Pernestig A.-K., Georgellis D., Romeo T., Suzuki K., Tomenius H., Normark S., Melefors O.
    J. Bacteriol. 185:843-853(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiBARA_ECOLI
AccessioniPrimary (citable) accession number: P0AEC5
Secondary accession number(s): P26607, P77032
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: July 6, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.