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Protein

Signal transduction histidine-protein kinase BarA

Gene

barA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histidine phosphotransfer kinase activity Source: EcoliWiki
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • cellular response to organic substance Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoliWiki
  • protein phosphorylation Source: EcoliWiki
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to hydrogen peroxide Source: EcoliWiki
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionKinase, Transferase
Biological processTranscription, Transcription regulation, Two-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:BARA-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transduction histidine-protein kinase BarA (EC:2.7.13.3)
Gene namesi
Name:barA
Ordered Locus Names:b2786, JW2757
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11367. barA.

Subcellular locationi

P0AEC5:

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 31HelicalSequence analysisAdd BLAST22
Topological domaini32 – 176PeriplasmicSequence analysisAdd BLAST145
Transmembranei177 – 196HelicalSequence analysisAdd BLAST20
Topological domaini197 – 918CytoplasmicSequence analysisAdd BLAST722

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000746971 – 918Signal transduction histidine-protein kinase BarAAdd BLAST918

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei302Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1
Modified residuei7184-aspartylphosphatePROSITE-ProRule annotation1
Modified residuei861PhosphohistidinePROSITE-ProRule annotation1

Post-translational modificationi

Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AEC5.
PRIDEiP0AEC5.

PTM databases

iPTMnetiP0AEC5.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
clpAP0ABH93EBI-546740,EBI-546140

Protein-protein interaction databases

BioGridi4262286. 16 interactors.
DIPiDIP-47932N.
IntActiP0AEC5. 4 interactors.
MINTiMINT-1307958.
STRINGi316385.ECDH10B_2954.

Structurei

3D structure databases

ProteinModelPortaliP0AEC5.
SMRiP0AEC5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini200 – 252HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini299 – 520Histidine kinasePROSITE-ProRule annotationAdd BLAST222
Domaini669 – 785Response regulatoryPROSITE-ProRule annotationAdd BLAST117
Domaini822 – 918HPtPROSITE-ProRule annotationAdd BLAST97

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG0784. LUCA.
COG2198. LUCA.
COG4999. LUCA.
HOGENOMiHOG000272329.
InParanoidiP0AEC5.
KOiK07678.
PhylomeDBiP0AEC5.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
cd00075. HATPase_c. 1 hit.
cd00082. HisKA. 1 hit.
cd00088. HPT. 1 hit.
cd00156. REC. 1 hit.
Gene3Di1.20.120.160. 1 hit.
3.30.565.10. 1 hit.
InterProiView protein in InterPro
IPR011006. CheY-like_superfamily.
IPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR036890. HATPase_C_sf.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR036097. HisK_dim/P_sf.
IPR019247. Histidine_kinase_BarA_N.
IPR036641. HPT_dom_sf.
IPR004358. Sig_transdc_His_kin-like_C.
IPR008207. Sig_transdc_His_kin_Hpt_dom.
IPR001789. Sig_transdc_resp-reg_receiver.
PfamiView protein in Pfam
PF09984. DUF2222. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF01627. Hpt. 1 hit.
PF00072. Response_reg. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiView protein in SMART
SM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00073. HPT. 1 hit.
SM00448. REC. 1 hit.
SUPFAMiSSF47226. SSF47226. 2 hits.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiView protein in PROSITE
PS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50894. HPT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AEC5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNYSLRARM MILILAPTVL IGLLLSIFFV VHRYNDLQRQ LEDAGASIIE
60 70 80 90 100
PLAVSTEYGM SLQNRESIGQ LISVLHRRHS DIVRAISVYD ENNRLFVTSN
110 120 130 140 150
FHLDPSSMQL GSNVPFPRQL TVTRDGDIMI LRTPIISESY SPDESPSSDA
160 170 180 190 200
KNSQNMLGYI ALELDLKSVR LQQYKEIFIS SVMMLFCIGI ALIFGWRLMR
210 220 230 240 250
DVTGPIRNMV NTVDRIRRGQ LDSRVEGFML GELDMLKNGI NSMAMSLAAY
260 270 280 290 300
HEEMQHNIDQ ATSDLRETLE QMEIQNVELD LAKKRAQEAA RIKSEFLANM
310 320 330 340 350
SHELRTPLNG VIGFTRLTLK TELTPTQRDH LNTIERSANN LLAIINDVLD
360 370 380 390 400
FSKLEAGKLI LESIPFPLRS TLDEVVTLLA HSSHDKGLEL TLNIKSDVPD
410 420 430 440 450
NVIGDPLRLQ QIITNLVGNA IKFTENGNID ILVEKRALSN TKVQIEVQIR
460 470 480 490 500
DTGIGIPERD QSRLFQAFRQ ADASISRRHG GTGLGLVITQ KLVNEMGGDI
510 520 530 540 550
SFHSQPNRGS TFWFHINLDL NPNIIIEGPS TQCLAGKRLA YVEPNSAAAQ
560 570 580 590 600
CTLDILSETP LEVVYSPTFS ALPPAHYDMM LLGIAVTFRE PLTMQHERLA
610 620 630 640 650
KAVSMTDFLM LALPCHAQVN AEKLKQDGIG ACLLKPLTPT RLLPALTEFC
660 670 680 690 700
HHKQNTLLPV TDESKLAMTV MAVDDNPANL KLIGALLEDM VQHVELCDSG
710 720 730 740 750
HQAVERAKQM PFDLILMDIQ MPDMDGIRAC ELIHQLPHQQ QTPVIAVTAH
760 770 780 790 800
AMAGQKEKLL GAGMSDYLAK PIEEERLHNL LLRYKPGSGI SSRVVTPEVN
810 820 830 840 850
EIVVNPNATL DWQLALRQAA GKTDLARDML QMLLDFLPEV RNKVEEQLVG
860 870 880 890 900
ENPEGLVDLI HKLHGSCGYS GVPRMKNLCQ LIEQQLRSGT KEEDLEPELL
910
ELLDEMDNVA REASKILG
Length:918
Mass (Da):102,453
Last modified:December 6, 2005 - v1
Checksum:iEA31D6D732F023CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10888 Genomic DNA. Translation: BAA01710.1.
U29580 Genomic DNA. Translation: AAA69296.1.
U00096 Genomic DNA. Translation: AAC75828.1.
AP009048 Genomic DNA. Translation: BAA16571.2.
PIRiS20550.
RefSeqiNP_417266.1. NC_000913.3.
WP_000186450.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75828; AAC75828; b2786.
BAA16571; BAA16571; BAA16571.
GeneIDi947255.
KEGGiecj:JW2757.
eco:b2786.
PATRICifig|1411691.4.peg.3949.

Similar proteinsi

Entry informationi

Entry nameiBARA_ECOLI
AccessioniPrimary (citable) accession number: P0AEC5
Secondary accession number(s): P26607, P77032
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: October 25, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene