Reviewed, UniProtKB/Swiss-Prot P0AEC5 (BARA_ECOLI)

Names and origin

Protein names

Recommended name:
    Signal transduction histidine-protein kinase barA
    EC=2.7.13.3

Gene names

Name:	barA
Ordered Locus Names:	b2786, JW2757

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	918 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Member of the two-component regulatory system uvrY/barA involved in the regulation of carbon metabolism via the csrA/csrB regulatory system. Phosphorylates uvrY, probably via a four-step phosphorelay.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.8.
Post-translational modification	Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain By similarity.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain. Contains 1 HPt domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW histidine phosphotransfer kinase activity Ref.5 Inferred from direct assay. Source: UniProtKB two-component response regulator activity Inferred from electronic annotation. Source: InterPro two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 918	918	Signal transduction histidine-protein kinase barA		PRO_0000074697
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 31	22	Potential
	Topological domain	32 – 176	145	Periplasmic Potential
	Transmembrane	177 – 196	20	Potential
	Topological domain	197 – 918	722	Cytoplasmic Potential
	Domain	200 – 252	53	HAMP
	Domain	299 – 520	222	Histidine kinase
	Domain	669 – 785	117	Response regulatory
	Domain	822 – 918	97	HPt
Amino acid modifications
	Modified residue	302	1	Phosphohistidine; by autocatalysis By similarity
	Modified residue	718	1	4-aspartylphosphate By similarity
	Modified residue	861	1	Phosphohistidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AEC5-1 [UniParc]. Last modified December 6, 2005. Version 1. Checksum: EA31D6D732F023CD	FASTA	918	102,453
	10 20 30 40 50 60 MTNYSLRARM MILILAPTVL IGLLLSIFFV VHRYNDLQRQ LEDAGASIIE PLAVSTEYGM 70 80 90 100 110 120 SLQNRESIGQ LISVLHRRHS DIVRAISVYD ENNRLFVTSN FHLDPSSMQL GSNVPFPRQL 130 140 150 160 170 180 TVTRDGDIMI LRTPIISESY SPDESPSSDA KNSQNMLGYI ALELDLKSVR LQQYKEIFIS 190 200 210 220 230 240 SVMMLFCIGI ALIFGWRLMR DVTGPIRNMV NTVDRIRRGQ LDSRVEGFML GELDMLKNGI 250 260 270 280 290 300 NSMAMSLAAY HEEMQHNIDQ ATSDLRETLE QMEIQNVELD LAKKRAQEAA RIKSEFLANM 310 320 330 340 350 360 SHELRTPLNG VIGFTRLTLK TELTPTQRDH LNTIERSANN LLAIINDVLD FSKLEAGKLI 370 380 390 400 410 420 LESIPFPLRS TLDEVVTLLA HSSHDKGLEL TLNIKSDVPD NVIGDPLRLQ QIITNLVGNA 430 440 450 460 470 480 IKFTENGNID ILVEKRALSN TKVQIEVQIR DTGIGIPERD QSRLFQAFRQ ADASISRRHG 490 500 510 520 530 540 GTGLGLVITQ KLVNEMGGDI SFHSQPNRGS TFWFHINLDL NPNIIIEGPS TQCLAGKRLA 550 560 570 580 590 600 YVEPNSAAAQ CTLDILSETP LEVVYSPTFS ALPPAHYDMM LLGIAVTFRE PLTMQHERLA 610 620 630 640 650 660 KAVSMTDFLM LALPCHAQVN AEKLKQDGIG ACLLKPLTPT RLLPALTEFC HHKQNTLLPV 670 680 690 700 710 720 TDESKLAMTV MAVDDNPANL KLIGALLEDM VQHVELCDSG HQAVERAKQM PFDLILMDIQ 730 740 750 760 770 780 MPDMDGIRAC ELIHQLPHQQ QTPVIAVTAH AMAGQKEKLL GAGMSDYLAK PIEEERLHNL 790 800 810 820 830 840 LLRYKPGSGI SSRVVTPEVN EIVVNPNATL DWQLALRQAA GKTDLARDML QMLLDFLPEV 850 860 870 880 890 900 RNKVEEQLVG ENPEGLVDLI HKLHGSCGYS GVPRMKNLCQ LIEQQLRSGT KEEDLEPELL 910 ELLDEMDNVA REASKILG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel sensor-regulator protein that belongs to the homologous family of signal-transduction proteins involved in adaptive responses in Escherichia coli." Nagasawa S., Tokishita S., Aiba H., Mizuno T. Mol. Microbiol. 6:799-807(1992) [PubMed: 1574005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 106-918. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Identification of UvrY as the cognate response regulator for the BarA sensor kinase in Escherichia coli." Pernestig A.-K., Melefors O., Georgellis D. J. Biol. Chem. 276:225-231(2001) [PubMed: 11022030] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[6]	"Regulatory circuitry of the CsrA/CsrB and BarA/UvrY systems of Escherichia coli." Suzuki K., Wang X., Weilbacher T., Pernestig A.-K., Melefors O., Georgellis D., Babitzke P., Romeo T. J. Bacteriol. 184:5130-5140(2002) [PubMed: 12193630] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[7]	"The Escherichia coli BarA-UvrY two-component system is needed for efficient switching between glycolytic and gluconeogenic carbon sources." Pernestig A.-K., Georgellis D., Romeo T., Suzuki K., Tomenius H., Normark S., Melefors O. J. Bacteriol. 185:843-853(2003) [PubMed: 12533459] [Abstract] Cited for: CHARACTERIZATION.
[8]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10888 Genomic DNA. Translation: BAA01710.1. U29580 Genomic DNA. Translation: AAA69296.1. U00096 Genomic DNA. Translation: AAC75828.1. AP009048 Genomic DNA. Translation: BAA16571.2.
PIR	S20550.
RefSeq	AP_003352.1. NP_417266.1.
3D structure databases
SMR	P0AEC5. Positions 285-518, 405-547, 527-786.
ModBase	Search...
Protein-protein interaction databases
STRING	P0AEC5.
Genome annotation databases
GeneID	947255.
GenomeReviews	Gene locus JW2757 in contig AP009048_GR. Gene locus b2786 in contig U00096_GR.
KEGG	ecj:JW2757. eco:b2786.
Organism-specific databases
EchoBASE	EB1341.
EcoGene	EG11367. barA.
CMR	Search...
Phylogenomic databases
eggNOG	COG0642.
HOGENOM	HBG298668.
OMA	VESAANQ.
Enzyme and pathway databases
BioCyc	EcoCyc:BARA-MONOMER. ECOL168927:B2786-MONOMER.
Gene expression databases
Genevestigator	P0AEC5.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011006. CheY-like. IPR019247. DUF2222. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR008207. Sig_transdc_His_kin_Hpt_dom. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF09984. DUF2222. 1 hit. PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. PF01627. Hpt. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. SM00073. HPT. 1 hit. SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. PS50894. HPT. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BARA_ECOLI

Accession

Primary (citable) accession number: P0AEC5
Secondary accession number(s): P26607, P77032

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)